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Citations for VWR :
301 - 350 of 462 citations for Poly rC Binding Protein 4 PCBP4 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Cell supernatants were collected at different time points and centrifuged 30min at 10,000 × g at 4°C to eliminate cell debris (centrifuge CT15RE, VWR), then at 100,000 × g for 3h to eliminate larger vesicles (L7-55 Ultracentrifuge ...
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bioRxiv - Immunology 2020Quote: These assays were done using methods similar to those described (52) and Immulon 4 microtiter plates (VWR, Radnor, PA) coated with 100ng per well of S1 ...
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bioRxiv - Microbiology 2022Quote: ... Brains and lungs were collected at 4-days post inoculation and homogenized in PBS with a benchtop homogenizer (VWR). Homogenates were diluted in a 10-fold dilution series and each dilution was plated on YPD ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were euthanized with euthasol and transcardially perfused with 0.3% glutaraldehyde-4% paraformaldehyde in PBS (0.1 M, pH 7.4; VWR Chemicals, France), and the neurobiotin-filled neurons revealed as previously described (Williams et al. ...
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bioRxiv - Immunology 2023Quote: ... then placed overnight at 4°C in a 25% sucrose solution containing 50% optimal cutting temperature compound (OCT, VWR Clear Frozen Section Compound ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were collected onto copper mesh grids and contrasted for 2min with 4% uranyl acetate solution in methanol (VWR), followed by 2min in lead citrate (Reynold’s solution) ...
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bioRxiv - Cancer Biology 2023Quote: ... at 37 °C for 25 min while stirring at 900 RPM (speed 4/6) on a hotplate stirrer (VWR). The digested suspension was strained through a 70-um strainer ...
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bioRxiv - Immunology 2024Quote: ... in Ce3D using Frame Seal incubation chambers (Bio Rad) and kept at 4°C in the dark after coverslipping (thickness no. 1.5; VWR). Slices were imaged on a Zeiss LSM 880 confocal microscope using a 32 channel Gallium arsenide phosphide (GaAsP ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR) for cohesin trimers and a HiLoad 16/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Protein cell lysates were collected by lysing and scraping cells with RIPA Lysis buffer (VWR, Mississauga, Ontario) mixed with protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... proteins were eluted using 0.1 M glycine at pH 3.5 and concentrated using Vivaspin spin filters (VWR) with appropriate MW cut-offs ...
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bioRxiv - Bioengineering 2020Quote: ... Then we denatured the phage protein coat by adding 7.5 ml of 3 M KOAc (VWR international), pH 5.5 ...
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bioRxiv - Biophysics 2022Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR). Peak fractions were collected ...
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bioRxiv - Neuroscience 2023Quote: ... The dots were normalized on protein concentration (BCA assay) and cell numbers (EVE Automated Cell Counter, VWR). Each drop contained 10ug protein in 5 ul RIPA ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to nitrocellulose overnight in 20% methanol in Tris/Glycine transfer buffer (VWR, #97061-382). Total protein was visualized using the LI-COR REVERT™ Total Protein Stain (Licor ...
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bioRxiv - Biochemistry 2023Quote: ... at which point protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) (VWR, #437145X) at the final concentration of 0.5 mM ...
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bioRxiv - Biophysics 2021Quote: ... Cell culture media growth factors and antibodies were purchased from VWR International PBI (Milan ...
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bioRxiv - Cell Biology 2023Quote: ... IRDye 800CW Goat-anti-Rabbit antibody (VWR, Cat No:102673-330) and IRDye 680RD Goat-anti-Mouse antibody (VWR ...
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bioRxiv - Developmental Biology 2023Quote: ... incubated with the secondary antibody and DAPI (1:1000; AppliChem, VWR) for 1 h at RT and mounted with Prolong™ Gold Antifade reagent (Cell Signaling Technology ...
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bioRxiv - Microbiology 2024Quote: ... Rpoβ (anti-RNA polymerase beta mouse monoclonal antibody [clone: 8RB13]; VWR) was diluted 1:20,000 ...
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bioRxiv - Microbiology 2020Quote: ... concentrated OMVs were diluted 4 times in dH2O and 5 μL of this dilution was added to 150 μL Bradford reagent (VWR). Absorbance was measured at 595 nm and compared to a standard curve to calculate protein concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were centrifuged at 14000 rpm at 4°C and the supernatants clarified with a 0.2 μm membrane syringe filter (VWR International) before proceeding with a two-step purification protocol ...
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bioRxiv - Microbiology 2019Quote: Colony morphology agar was prepared as described above and 4 mL of the mixture were poured into 35 mm×10 mm round Petri dishes (25373-041, VWR)and left to cool and solidify overnight (14 to 24 h ...
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bioRxiv - Microbiology 2019Quote: ... The membranes were washed 3 times in TBST for 15 minutes each and blots were developed with BCIP/NBT (5-bromo-4-chloro-3-indoyl-phosphate/nitro blue tetrazolium) color development substrate (VWR).
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bioRxiv - Biophysics 2019Quote: ... (4) four times BRB40 buffer (40 mM Pipes [Sigma], pH 6.84, with KOH [VWR], 1mM EGTA [Sigma], 4mM MgCl2 [VWR]) to remove excess F-127 in solution and exchange buffers ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were washed three times with PBST and then incubated with 4′,6-diamidino-2-phenylindole (DAPI, 5 µg/mL in PBS) (VWR) and phalloidin (1.5 µM in DMSO ...
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bioRxiv - Biochemistry 2020Quote: Frozen hippocampus brain tissues were homogenized in a 4 volume of the 10% trichloroacetic acid (VWR International, Radnor, PA, USA) solution using a Kontes glass tissue grinder ...
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bioRxiv - Biophysics 2021Quote: ... cells were plated at a density of 4×104 cells/cm2 in a 5-layer cell chamber (VWR 76045-402) to yield enough material for completing the experiment ...
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bioRxiv - Microbiology 2020Quote: ... and 1 volume of phenol (pH 4.3):chloroform (1:1), followed by centrifugation (16000 x g, 4 °C, 10 min) in a phase lock tube (Quantbio, VWR) to separate the aqueous and organic phases ...
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bioRxiv - Neuroscience 2020Quote: ... and then cryoprotected for 3 days at 4 °C in 30% sucrose solution (w/v in 1× PBS; VWR Chemicals). Brains were then embedded into optimal cutting temperature (OCT ...
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bioRxiv - Genomics 2021Quote: Tissue sections were prepared from formalin-fixed paraffin embedded tissue blocks and cut to 4 micrometers serial sections and mounted on Superfrost Plus (VWR). The procedure for multiplex immunohistochemistry (mIHC ...
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bioRxiv - Physiology 2021Quote: ... on a shaker at 4°C in tris-buffered saline with 1% tween 20 (TBS-T; VWR Amresco ProPure M147) and 0.1 g ml-1 skim milk powder (Kroger ...
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bioRxiv - Plant Biology 2021Quote: ... one of tissue piece (50 mg) was ground using a 4-mm steel ball in a 2-ml Eppendorf tube in a bead beater (VWR) for 1 min at 30 revolutions·s−1 ...
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bioRxiv - Biophysics 2020Quote: ... The cell lysate was separated from insoluble material by centrifugation at 40,000 g for 60 minutes at 4 °C and subsequently incubated with a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C on a rotator for affinity chromatography via the 6xHis-tag ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated at 4℃ to maintain their viability and passed through a 40 µm cell strainer (VWR, 76327-098) to dissociate single cells from clumps ...
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bioRxiv - Biochemistry 2019Quote: ... microalgae were harvested via centrifugation at 13G (9000 rpm) for 20 mins 4°C using sterile 250 ml centrifuge tubes (VWR) and concentrated in a 50 ml sterile tubes (VWR ...
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bioRxiv - Cell Biology 2021Quote: ... After solidification of the gelatin block for 6h at 4°C it was embedded in a larger OCT block (Qpath, VWR) and subjected to cryosectioning (10 µm ...
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bioRxiv - Cell Biology 2020Quote: Melanocytes were cultured in 10-cm dishes and fixed in situ with Karnovsky’s fixative [4% paraformaldehyde (VWR Scientific, Radnor, PA), 4 mM calcium chloride ...
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bioRxiv - Cancer Biology 2021Quote: ... ∼1 in wide and 3-4 in long into a 15 cm petri dish (CA25383-103, VWR, Edmonton, AB, Canada) and created spherical depressions ∼2.5-3 mm in diameter to create a mold for the Matrigel ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris precast gels (Fisher Scientfic, WG1402BOX) and then transferred to 0.45μM nitrocellulose membranes (VWR, 10120-006). Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Blood plasma was obtained by centrifuging at 5000 rpm for 10 minutes at 4 °C and stored in cryotube vials (VWR Micro-centrifuge tubes with graduation 2.0 mL ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Jag1-Fc was immobilized by overnight incubation at 4°C in individual wells of non-tissue culture-treated 6 or 12 well plates (VWR) at a final concentration of 2 µg/mL in DPBS containing 10 µg/mL poly-D-lysine (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: The strains were grown overnight in M9 minimal media (4 g/L glucose, 1 g/L casamino acids (cat. no 2240, VWR), and 1 mg/L thiamine (cat ...
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bioRxiv - Physiology 2024Quote: ... were then exposed to cold treatments of -15°C for up to 48 hours (in increments of 4, 8, 12, or 48 hours) in a cooling circulator (VWR Scientific 1167P Heating/Cooling Recirculating Water Bath ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals ...
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bioRxiv - Biophysics 2023Quote: HeLa cells were fixed using 4% paraformaldehyde and permeabilized using Triton-X100 (VWR, Radnor, PA, USA, Cat. No. M143-1L) 12 hours after TTLL6-YFP transfection ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
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bioRxiv - Neuroscience 2023Quote: ... washed once in ice-cold 1× PBS with Ca2+/Mg2+ and then fixed in 4% paraformaldehyde (PFA, VWR: 100504-940) for 30 mins at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... all proteins were diluted in filtered (sterile syringe filters 0.45 μm cellulose acetate membrane, VWR International, Radnor, USA) Min buffer in the presence of either 0.5 mM ADP or ATP ...
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bioRxiv - Genetics 2020Quote: Quadriceps from P10 pups were dissected and total proteins extracted using radioimmunoprecipitation assay (RIPA) buffer (VWR, Radnor, PA) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific) ...