Labshake search
Citations for VWR :
101 - 150 of 200 citations for PCR Product Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Samples (1 μL) were added to reaction mixtures (19 μL) in a 96-well white PCR plate (VWR) on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were immediately flash frozen in liquid nitrogen in individual 0.2ml thin wall PCR tubes (731-0679, VWR) and stored at -80°C until further use (Supplementary Figure S1) ...
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bioRxiv - Genetics 2020Quote: ... Tissue DNA isolation kit (Omega Biotek, VWR). For fat pad and synovium ...
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bioRxiv - Genetics 2020Quote: ... DNA/RNA isolation kit (Omega Biotek, VWR). For blood ...
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bioRxiv - Microbiology 2019Quote: Thirty μl of solution A was dispensed into transparent vessels such as PCR tubes (VWR International catalog # 20170-004) or microtiter wells ...
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bioRxiv - Bioengineering 2022Quote: ... combining 1 μL sample with 19 μL reaction mixture per replicate in a 96-well white PCR plate (VWR). For amplicon detection ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR was performed on previously generated cDNA (8 ng) using KOD Hot Start DNA Polymerase (VWR CA80511-386). The primer sequences for the target transcripts and number of cycles are outlined in Table 3 ...
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bioRxiv - Cell Biology 2022Quote: ... according to manufacturer’s recommendations and was added as a template into a one-step multiplex qRT-PCR assay using Quanta qScript XLT ToughMix with ROX dye (VWR). For that ...
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bioRxiv - Synthetic Biology 2020Quote: ... reactions were incubated in PCR tubes prior to irradiation using a UV-transilluminator (VWR, Dual UV Transilluminator, 80 mW/cm2). Following irradiation ...
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bioRxiv - Molecular Biology 2023Quote: 10 µg of purified RNA samples for each condition (wt and rie1 delete) was transferred to a PCR tube (VWR). Then 1.7 µl of primer homologous to IME1 was added to each tube ...
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bioRxiv - Molecular Biology 2024Quote: ... 15 μL of 0.6 mg/mL HCMV gB was mixed with 5 μL of dilute SYPRO Orange working solution in white-walled 96-well PCR plates (VWR), which were then sealed and loaded into a LightCycler 480 differential scanning fluorimeter (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... DAB (Bright-DAB Substract Kit, IL ImmunoLogic, VWR) was used for protein detection ...
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bioRxiv - Microbiology 2023Quote: ... subtilis using the peqGOLD Bacterial DNA Kit (VWR). Plasmid DNA was isolated using the Nucleospin Extract Kit (Macherey-Nagel) ...
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bioRxiv - Biochemistry 2024Quote: ... peqGOLD Plasmid Miniprep Kit I (VWR Life Science). Successful mutagenesis was verified by Sanger sequencing.
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bioRxiv - Bioengineering 2021Quote: ... 100 μL of dye at each concentration were aliquoted into a strip of 12 optically-clear PCR tubes (VWR 93001-118). The instrument was allowed to reach a steady-state temperature of 69° C before the first strip of tubes was loaded ...
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bioRxiv - Bioengineering 2021Quote: ... antibiotic-resistant colonies were re-streaked and verified by colony PCR using specific primers and Red Taq DNA polymerase master mix (VWR International).
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bioRxiv - Biophysics 2023Quote: ... the top and bottom strand were annealed using a PCR machine (Doppio, VWR®-thermocyclerseries, VWR, part of Avantor, Radnor, US) increasing the temperature to 95 °C (3 min ...
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bioRxiv - Microbiology 2024Quote: ... An aliquot (24.5 µL) of the master mix was added to all wells of a 96-well PCR plate (VWR, Radnor, PA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Minipreps were performed using a peqGold miniprep kit (VWR). WMISH probes were generated via a SP6 or T7 transcription kit (NEB ...
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bioRxiv - Genomics 2024Quote: The peqGOLD Tissue DNA Mini Kit (VWR Life Science) was used to extract genomic DNA after genome editing in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Digestion and ligation efficiencies were assessed by gel electrophoresis on a 0.9% agarose gel and quantitative PCR (Brilliant III SYBR qPCR Master Mix, VWR Cat # 97066-528).
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bioRxiv - Bioengineering 2021Quote: ... A 15-µl aliquot of the master mix was combined with 10 µl of sample in a 96-well PCR plate (VWR, 83007-374). The plate was mixed on a Qiagen TissueLyser II for two 25-second intervals at 13 Hz then centrifuged for 2 minutes at 1000 xg.
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bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells were treated either with 800 μM OPA or corresponding concentration of DMSO and heated in parallel in a PCR (VWR, Doppio Gradient) block for 15 min to the three temperatures (37°C ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated using the peqGOLD Total RNA Kit (VWR) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... the exons of interest in PD-L1 gene were amplified by using specific primers (Table 5) with a PCR thermal cycler (VWR Thermocycler, Leicestershire, England). PCR procedures were performed with a standard reaction mixture (4 μl/sample of Phusion GC Buffer (Thermo Fischer Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... were prepared and stored in 0.2 mL snap strip PCR tubes with dome caps (490003-692, GeneMate – BioExpress, VWR International, Brisbane, CA, USA) at -20 °C prior to qRT-PCR plates preparation ...
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bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...
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bioRxiv - Neuroscience 2022Quote: RNA was isolated using TriFast RNA extraction kit (30-2030, VWR) or RNA-Solv reagent (Omega Bio-tek) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Total RNA Kit I (Omega BIO-TEK; VWR catalog no. 101319) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The COD was determined using the Spectroquant COD kit (VWR, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... extracted with the RNA extract kit (1x106 cells-10mg; VWR International PBI) and retrotranscribed with iScript Supermix (Bio-Rad Laboratories) ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted using the Zymo Quick-RNA MiniPrep kit (VWR, R1055) according to the manufacturers protocol ...
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bioRxiv - Genetics 2020Quote: ... pelleted and purified by the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA). Inserts of the isolated plasmids were verified by sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... Total DNA was isolated using Plant DNA mini Kit (Peqlab/VWR, Darmstadt, Germany). To quantify fungal or bacterial DNA content ...
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bioRxiv - Microbiology 2021Quote: ... histolytica expression plasmid pEhEx backbone (38) using the Gibson Assembly Ultra Kit (VWR). Primers used for Gibson cloning are described in Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted using the Zymo Quick-RNA miniprep kit (VWR; R1055) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was extracted using the Direct-zol RNA miniprep kit (VWR 76211-340). cDNA synthesis was performed using hexamer primers and SuperScript IV Reverse Transcriptase (Thermo Fisher 18090010) ...
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bioRxiv - Molecular Biology 2022Quote: ... The total soma RNA was extracted with the RNA extraction kit (VWR International PBI) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... DNA was extracted from the gel slices using the peqGOLD Gel Extraction Kit (VWR). Genotyping was done using a modified ast-PCR protocol for R21X (Supplemental methods ...
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bioRxiv - Bioengineering 2022Quote: ... was incubated for 30 minutes at RT and Vector VIP Peroxidase Substrate Kit (VWR) was used to develop staining ...
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bioRxiv - Cell Biology 2022Quote: ... reverse transcription was performed using the qScrpit cDNA synthesis kit (VWR, catalog # 95048-100) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was isolated from the cells with Zymo Quick-RNA Miniprep kit (VWR, #76299-632) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA was amplified and purified using EZNA endo-free plasmid maxi kit (VWR International).
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bioRxiv - Immunology 2021Quote: ... The pool was quantified using the Peqlab KAPA Library Quantification Kit (VWR, Radnor, PA; USA) and the Applied Biosystems 7900HT Sequence Detection (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were monitored for Mycoplasma contamination regularly using Mycoplasma Detection Kit (VWR, 89510-164).
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bioRxiv - Molecular Biology 2022Quote: ... DNA was digested according to the peqGOLD DNase I Digest kit (VWR 13-1091-01), followed by washing steps with 400 µL RNA Prep Buffer (ZymoResearch R1060-2 ...
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bioRxiv - Immunology 2021Quote: ... The pool was quantified using the Peqlab KAPA Library Quantification Kit (VWR, Radnor, PA; USA) and the Applied Biosystems 7900HT Sequence Detection (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... 5ml of curing agent and 45ml of Monomer (both Sylgard® 184 Elastomer Kit, VWR) were mixed intensively ...