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Citations for VWR :
501 - 543 of 543 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... by loading 5µl of each PCR product onto a 1% agarose gel with 1:10,000 Gel Red™(Biotium/VWR®) and run for 20 minutes at 100 volts ...
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bioRxiv - Molecular Biology 2023Quote: 10 µg of purified RNA samples for each condition (wt and rie1 delete) was transferred to a PCR tube (VWR). Then 1.7 µl of primer homologous to IME1 was added to each tube ...
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bioRxiv - Developmental Biology 2023Quote: ... All PCR products were separated by electrophoresis on a 2% agarose gel in TBS stained with ethidium bromide (VWR, E3050), and genotypes determined based on sizes of DNA products.
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bioRxiv - Cell Biology 2020Quote: ... Solutions were mixed by shaking the compound plate for 1 min at room temperature at 1200 rpm using a microplate shaker (cat. 12620-926, VWR, West Chester, PA). A typical plate layout map used for screening experiments is shown in Figure 1 ...
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bioRxiv - Biophysics 2019Quote: ... cells were grown in 6-well plates (STARLAB; Milton Keynes, UK) or 30 mm culture dishes (STARLAB) on untreated 30 mm borosilicate coverslips (Thickness # 1, VWR International; Radnor, PA) or on poly-D-lysine coated FluoroDishes (FD35-PDL-100 ...
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bioRxiv - Bioengineering 2021Quote: ... Microtiter plates were filled with 160 μl of bloodmeal per well and sealed with a membrane (Parafilm M, PM999, VWR, Amsterdam, the Netherlands) that was stretched to about 250% its original dimensions in both directions using a custom build device (Figure S1A ...
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bioRxiv - Cell Biology 2019Quote: ... Single cells were sorted into 200 μL fresh culture medium in one well of a 96-well flat bottom plate (VWR Catalog #29442-054).
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bioRxiv - Cancer Biology 2021Quote: 1×105 A549 or H460 cells in 200 µl of serum-free growth medium were seeded in the insert of a transwell plate with 8 μm pores (VWR International, Radnor, PA). These cells were coincubated with AvFc or CTX at 30 nM for 2 hours at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... from control and treatment cultures were harvested at both 24- and 48-hour time points and mixed with 400μL of culture media in a 96-deep well storage plate (VWR International, Radnor, PA, USA) and stored frozen at −20°C for cytokine assays by ELISA ...
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bioRxiv - Microbiology 2021Quote: ... Vero cells were seeded at 60% confluence in 24-well plates equipped with 13 mm round glass coverslips (VWR, cat n. 631-0150). Cells were washed once with PBS before incubation with BetaCoV/Australia/VIC01/2020 diluted in PBS at an MOI=5 ...
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bioRxiv - Immunology 2020Quote: ... Fused cells were then seeded on 96 well flat bottom plates onto mouse peritoneal cells as feeder layer in hypoxanthine-aminopterine-thymidine (VWR, Radnor, Pennsylvania, USA) medium for the selection process.
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bioRxiv - Genetics 2019Quote: We performed cell culture using the conditions described above in 12-well plates containing sterile 18-mm glass cover slips (VWR, Cat#48380-046). We fixed cells for 10 minutes in 4% paraformaldehyde (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: Antibiotic tolerance assays using co-cultures were performed using a membrane-separated 12-well tissue plate cultures containing 0.1 μm pore PET membranes (VWR, Cat. No. 10769–226), following previously protocols (Meirelles et al. ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of the enriched seawater and 100 μL of the diatom-concentrated seawater were then inoculated on agar plates (1% agar – VWR Agar for bacteriology) supplemented with Marine Broth 2216 (VWR ...
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bioRxiv - Genomics 2022Quote: ... HeLa S3 or HeLa S3 + TOP2A- Venus cell linescells were either grown or spun onto 22×22mm No 1.5 coverslips in 6-well plates (VWR Cat No 48366-277). (cell cycle analysis experiments ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (4×105 cells/well) were laid in sterile round bottom 96-well tissue culture treated plates (VWR, The Netherlands, cat #734-2184) in Dutch modified RPMI 1640 medium containing 50 µg/mL Gentamicine ...
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bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
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bioRxiv - Biochemistry 2024Quote: ... 200 µL aliquots were then transferred to the wells of a NUNC flat-bottomed 96-well microtiter plate (VWR International GMBH, Darmstadt, Germany). Subsequently ...
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bioRxiv - Microbiology 2024Quote: The bacterial strains were revived by streaking a loopful of frozen glycerol stock on Brain Heart Infusion (BHI) agar plates (15 g/L Agar, 37 g/L BHI Broth, VWR Life Science, USA) and incubated at 37±2 °C overnight ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... A single colony of bacteria from each plate was cultured under anaerobic conditions (Anaerobic Hungate Culture Tubes with air displaced by CO2, VWR, Radnor, PA, USA), shaking at 35°C for 3 nights and was then retained at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... 100 μL of dye at each concentration were aliquoted into a strip of 12 optically-clear PCR tubes (VWR 93001-118). The instrument was allowed to reach a steady-state temperature of 69° C before the first strip of tubes was loaded ...
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bioRxiv - Bioengineering 2021Quote: ... antibiotic-resistant colonies were re-streaked and verified by colony PCR using specific primers and Red Taq DNA polymerase master mix (VWR International).
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bioRxiv - Microbiology 2023Quote: ... Then all PCR products were run on 1.5% agarose gel and the PCR product concentrations were assessed based on gel band intensity using GENOSOFT software (VWR International, Belgium). By their concentrations ...
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bioRxiv - Biophysics 2023Quote: ... the top and bottom strand were annealed using a PCR machine (Doppio, VWR®-thermocyclerseries, VWR, part of Avantor, Radnor, US) increasing the temperature to 95 °C (3 min ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were visualised on a 1% agarose gel (run for 30 minutes at 135 V) with GelGreen (VWR, 730-1535) using an ImageQuant™ LAS 500 (GE Lifesciences) ...
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bioRxiv - Microbiology 2020Quote: ... The plates were incubated for four hours at 37 °C under shaking conditions (175 rpm) using a benchtop incubator (VWR® incubator orbital shaker). The 96-well plate was kept inside an airtight plastic container with several wet paper towels to maintain high humidity attached to the shaker ...
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bioRxiv - Microbiology 2020Quote: ... co-culture experiments were performed using membrane-separated 12-well tissue plate cultures containing 0.1 µm pore PET membranes (VWR® Cat. No. 10769-226). Briefly ...
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bioRxiv - Microbiology 2019Quote: Online monitoring of growth and/or pH was performed in 48-well microtiter Flower Plates® (m2p-labs GmbH, Baesweiler, Germany) sealed with sterile breathable rayon films (VWR International, Pennsylvania, US) in a BioLector® microtiter cultivation system (m2p-labs GmbH ...
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bioRxiv - Immunology 2022Quote: Splenic T-lymphocytes were counted and plated at an optimal seeding density of 8×104 cells per 100 µL in 96 well Microtitration ImageLock plates (VWR, #4379, Radnor, PA, USA). The cells were cultured with anti-CD3 and anti-CD28 activation beads and imaged over the course of 96hrs in the Essen Bioscience IncuCyte S3 Live-Cell imager and analysis system.
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bioRxiv - Synthetic Biology 2022Quote: ... 1 μL volumes are taken from this tray using a multipipettor and used to inoculate wells of a 96-well plate (VWR, catalog number 10062-900) with 200μL volumes of H media ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μL of the enzyme-silicon mixture was spread on the bottom of wells in a clear flat bottom 96-well plate and placed on an oscillating shaker (VWR International, Radnor, PA, USA) for 5 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Viable embryos at the 2-cell stage (0.75 hours post fertilization (hpf)) were sorted into 4 replicates of 10 embryos within 24-well plates (VWR international, LLC Radnor, PA, USA). Replicates were exposed to 1 mL of vehicle (0.1% DMSO ...
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bioRxiv - Biophysics 2024Quote: ... 293T/17 cells were plated in complete DMEM at 8 × 105 cells per well in a 6-well plate with an autoclaved glass coverslip (VWR, no. CLS-1760-025) to ensure 80% confluency on the day of the experiment ...
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bioRxiv - Genomics 2020Quote: ... Digestion and ligation efficiencies were assessed by gel electrophoresis on a 0.9% agarose gel and quantitative PCR (Brilliant III SYBR qPCR Master Mix, VWR Cat # 97066-528).
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bioRxiv - Pathology 2021Quote: Viral RNA was quantitated using single-step RT-quantitative real-time PCR (Quanta qScript One-Step RT-qPCR Kit, QuantaBio, Beverly, MA; VWR; #76047-082) with primers and TaqMan® probes targeting the SARS-CoV-2 E gene as previously described (62) ...
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bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells were treated either with 800 μM OPA or corresponding concentration of DMSO and heated in parallel in a PCR (VWR, Doppio Gradient) block for 15 min to the three temperatures (37°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Equal volumes (5 μL) of the resulting PCR product were loaded onto a 2% biotechnology grade Agarose I (VWR International, PA, USA) gel prepared with 1X TBE buffer and 0.0025% ethidium bromide ...
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bioRxiv - Cancer Biology 2020Quote: ... the exons of interest in PD-L1 gene were amplified by using specific primers (Table 5) with a PCR thermal cycler (VWR Thermocycler, Leicestershire, England). PCR procedures were performed with a standard reaction mixture (4 μl/sample of Phusion GC Buffer (Thermo Fischer Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... were prepared and stored in 0.2 mL snap strip PCR tubes with dome caps (490003-692, GeneMate – BioExpress, VWR International, Brisbane, CA, USA) at -20 °C prior to qRT-PCR plates preparation ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.5 (Applichem) and transferred to a 30 kDa AcroPrep Omega filter membrane plate (PALL, New York, US, purchased via VWR, Hannover, Germany, REF 8035/518-0028) in a blocked randomized order ...
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bioRxiv - Microbiology 2023Quote: ... Six to seven grams (6 ml) of soil was transferred to each well of the 12-well microtiter plates (VWR American cat no.:10861-556, USA), containing three one-millimeter holes in the bottom of all wells for drainage ...
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bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...