Labshake search
Citations for VWR :
1 - 50 of 74 citations for Mouse MOB1B shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... cells expressing the various shRNAs were selected with 0.2 mg/ml hygromycin (cat#K547-20ml, VWR) at 72-hours post transfection and maintained at 0.8 mg/ml of hygromycin after cell line purification.
-
bioRxiv - Microbiology 2023Quote: Plasmid extraction (Omega, VWR), endonuclease digestion (New England Biolabs) ...
-
bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA was amplified and purified using EZNA endo-free plasmid maxi kit (VWR International).
-
bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...
-
bioRxiv - Genetics 2022Quote: ... or hygromycin (FIREWach plasmids, 200 ng/μl, VWR) starting two days after transduction ...
-
bioRxiv - Biochemistry 2024Quote: ... peqGOLD Plasmid Miniprep Kit I (VWR Life Science). Successful mutagenesis was verified by Sanger sequencing.
-
bioRxiv - Immunology 2022Quote: ... gentamycin (10 μg/ml for plasmid maintenance in E. coli; 100 μg/ml for plasmid maintenance in P. aeruginosa, VWR, Cat. No. 97062-974).
-
bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal (VWR GenScript A01622-40); western blot ...
-
bioRxiv - Molecular Biology 2022Quote: ... and anti-mouse (1:10,000; VWR)-conjugated to horseradish peroxidase HRP.
-
bioRxiv - Molecular Biology 2024Quote: ... secondary antibody (goat-anti-mouse-Cy3: VWR-95040-042 or goat-anti-rabbit-cy5 or Donkey anti-chicken-488 ...
-
bioRxiv - Genetics 2020Quote: ... pelleted and purified by the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA). Inserts of the isolated plasmids were verified by sequencing ...
-
bioRxiv - Cell Biology 2022Quote: Plasmids were transfected using jetPRIME® transfection reagent (VWR Scientific Cat # 89129-920) or Lipofectamine™ 2000 Transfection Reagent (Thermo Fisher Scientific #11668027 ...
-
bioRxiv - Microbiology 2021Quote: ... histolytica expression plasmid pEhEx backbone (38) using the Gibson Assembly Ultra Kit (VWR). Primers used for Gibson cloning are described in Table 1 ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmids were maintained in liquid culture with the appropriate antibiotics (VWR, Radnor, PA). List of plasmids are in Table S1.
-
bioRxiv - Systems Biology 2022Quote: Yeast with plasmids were grown in a 96-well deep well plate (VWR) containing 100μl of SC-Ura medium with 2% glucose and grown for ~12 hours at 30°C in a shaking incubator set at 250 rpm ...
-
bioRxiv - Molecular Biology 2024Quote: ... along with VSV-G and pUVMC packaging plasmids via Jetpei (VWR 89129-916).
-
bioRxiv - Cell Biology 2022Quote: ... anti-mouse (sheep, VWR International Ltd (NA931-1M)).
-
bioRxiv - Systems Biology 2019Quote: ... anti-mouse IgG (GE Healthcare, distributed by VWR in Ismaning ...
-
bioRxiv - Biophysics 2023Quote: ... and GAPDH (1:5000, 101983-284 mouse, VWR). Membranes were then washed and incubated with secondary antibodies for 1 hour (both 1:10,000 ...
-
bioRxiv - Neuroscience 2023Quote: ... goat-α-mouse-CF568 (1:500, VWR; AB_10559187), and goat-α-guinea pig-AlexaFluor647 (1.500 ...
-
bioRxiv - Microbiology 2021Quote: ... mouse monoclonal anti-GAPDH (VWR 1E6D9; 1:5,000 dilution), rabbit polyclonal anti-ACAT1 (Cell Signaling 44276 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and IRDye 680RD Donkey Anti-Mouse (VWR, #102673-412) were added at 1/5000 in blocking solution for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... or anti-mouse HRP-linked (VWR, cat. no NA931) antibodies at 1:1000 dilution (table 2) ...
-
bioRxiv - Physiology 2022Quote: ... the isolation was performed according to the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA) and verified through sequencing.
-
bioRxiv - Physiology 2022Quote: Mouse pancreas was weighed before fixing in Z-fix (VWR) and embedded with paraffin ...
-
bioRxiv - Genetics 2020Quote: HCT116 cells were transfected with px459sgCTNNB1 and the two donor template encoding plasmids KOZAK_FLAG_mClover3 and KOZAK_V5_mCherry using TransIT (731-0029; VWR) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
-
bioRxiv - Microbiology 2024Quote: ... Rpoβ (anti-RNA polymerase beta mouse monoclonal antibody [clone: 8RB13]; VWR) was diluted 1:20,000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... RNA was isolated from mouse liver with peqGOLD RNAPure reagent (VWR, USA) and QIAshredder spin columns (Qiagen ...
-
bioRxiv - Physiology 2021Quote: Freshly dissected mouse tissues were rinsed in PBS (VWR, cat. No. 437117K) and then immediately transferred into ice-cold fixative solution of 4% paraformaldehyde (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... and IRDye 680RD Goat-anti-Mouse antibody (VWR, Cat No:102673-408) at 1:20,000 dilutions ...
-
bioRxiv - Immunology 2023Quote: Mouse tissues were homogenized using soft tissue homogenizing beads (VWR, Radnor, PA). Total RNA was isolated using TRIzol® (Life Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... we transfect the cells with either WT or mutant SOD1 pCI-Neo plasmid using JetOptimus transfection reagent (VWR) according to the manufacturer’s instructions (31) ...
-
Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.
-
bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
-
bioRxiv - Microbiology 2022Quote: ... Each transformation consisted of 40μL of competent cells and 10ng of the pFAB5516 plasmid library transferred to a chilled cuvette (1mm gap, VWR). Cuvettes were electroporated using a BTX™-Harvard Apparatus ECM™ 630 Exponential Decay Wave Electroporator with the following parameters ...
-
bioRxiv - Genetics 2020Quote: Plasmid DNA containing the repair template with three tandem attP sites was co-transfected (1:1, Jetprime VWR#89129) with a plasmid containing Rosa26-Cas9/gRNA into mouse ES cells when confluency reached 70% ...
-
bioRxiv - Cell Biology 2023Quote: ... donkey anti-mouse IgG conjugated to IRDye 800CW (VWR, cat. no. 102673-332) or goat anti-rabbit IgG conjugated to IRDye 680RD (VWR ...
-
bioRxiv - Molecular Biology 2023Quote: ... Nitrocellulose membrane was then incubated with goat Anti-mouse (102971-154, VWR International) and goat Anti-rabbit (103011-498 ...
-
bioRxiv - Immunology 2024Quote: ... Mouse tumor sections were mounted on microscope cover glasses (#631-0170, VWR, France) using Fluoromount-G (#00-4958-02 ...
-
bioRxiv - Microbiology 2021Quote: ... Antibodies were produced by co-transfecting paired heavy- and light-chain antibody-expressing plasmids at a ratio of 1:1 using FectoPRO DNA Transfection Reagent (VWR) into Expi293 cells ...
-
bioRxiv - Genetics 2019Quote: ... Four million S2 cells that were stably transduced with RasV12Common and RasV12Rare plasmids were seeded into coated tissue culture dishes (60×15mm, VWR) with 2 ml of Schneider’s media (without FBS) ...
-
bioRxiv - Genetics 2021Quote: The 1.8 SRE-Elk cell line was generated by lentiviral transduction of 1.8 XX mESCs with the pLenti-SRE/Elk-GFP-PEST-Hygro plasmid (Supp. Table S4) followed by Hygromycin (250 ng/µl, VWR) selection ...
-
bioRxiv - Molecular Biology 2023Quote: ... raw HEK cells were transfected with the respective plasmid by applying the jetPRIME transfection protocol (VWR International GmBH, Vienna, Austria). Adding 100 µl geneticin (G418 ...
-
bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium ...
-
bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
-
bioRxiv - Cancer Biology 2020Quote: ... mouse anti-cavin3 (Cat no. ABNOH00112464-MO4, 1:200 PLA, VWR International Pty, Australia), rabbit anti-BRCA1 C-20 (SC-642 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
-
bioRxiv - Cell Biology 2023Quote: Mouse skin and wound beds were embedded in optimum cutting temperature (OCT) compound (VWR) and wound beds were sectioned through their entirety to identify the center ...