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Citations for VWR :
101 - 150 of 234 citations for Mouse Anti Human Papilloma virus types 16 18 716 F10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The V4 region of the 16S rRNA gene was amplified using Five prime hot master mix (VWR, Australia) using primers with custom barcodes ...
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bioRxiv - Biophysics 2021Quote: ... and subsequently incubated at 37 °C and 5% CO2 for 2 h before seeded and imaged on an 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ (Gibco ...
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bioRxiv - Bioengineering 2022Quote: ... cells for both batches were seeded onto the material at a confluence comprised between 50% and 60% on fibronectin coated (15 μg/ml in PBS buffer solution) 18-mm round glass coverslip (VWR, Radnor, USA). The cells were maintained in incubator at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2022Quote: ... medium was replaced by 60 μL of 5 mg/mL Collagen Type I (VWR, 734-1085) in 1M NaOH and 1X DMEM/F12 ...
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bioRxiv - Biophysics 2022Quote: ... LB broth and ampicillin for wild type (WT) α-Syn purification were purchased from VWR (Denmark). Purified Alexa-488 maleimide labeled 140C-α-Syn (24 μM stock solutions prepared in 10 mM sodium phosphate buffer (NaH2PO4.H20+Na2HPO4.2H20 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then rinsed 3x in TBS and incubated for 1 hour in anti-mouse IR680 (VWR 102971-020) and anti-rabbit IR800 (VWR 103011-494 ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Genetics 2019Quote: We performed cell culture using the conditions described above in 12-well plates containing sterile 18-mm glass cover slips (VWR, Cat#48380-046). We fixed cells for 10 minutes in 4% paraformaldehyde (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
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bioRxiv - Microbiology 2021Quote: ... difficile strains containing the phiLOV2.1 reporter plasmid were grown anaerobically at 37°C in BHIS-Tm10 for 12 - 16 h and inoculated 1:50 into fresh BHIS-Tm10 containing indicated treatments (1.70 µg mL−1 vancomycin (VWR), 0.67 µg mL−1 fidaxomicin (Cayman Chemical) ...
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bioRxiv - Bioengineering 2021Quote: ... HI-FBS was then centrifuged at 100,000 x g for at least 16 h and the supernatant was filtered through a 0.20 μm bottle top filter (VWR) for subsequent use in BDMSC media.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were serum-starved 16 hours prior to plating with 1% bovine serum albumin (VWR, Cat. no. 0332-100G) added for protein supplementation ...
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bioRxiv - Bioengineering 2021Quote: ... mixed with 300 μl of digestion buffer in a new Eppendorf tube and placed at 60°C (16 hrs, Thermormixer Compact F 1.6 A,VWR) for digestion ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were carefully dissected before immersion fixation for 16-24 hours in 10% neutral buffered formalin (VWR, Radnor, PA) and cryoprotection in 30% sucrose for 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... and DNA was precipitated by adding 1/10th volume 3 M sodium acetate pH 5.2 and 1 volume isopropanol (3032-16, VWR) then incubating at 22°C for 18 hr ...
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Cell Biology 2019Quote: ... The donkey anti-rabbit (NA934, 1:1000) and anti-mouse (NA931, 1:1000) horseradish peroxidase (HRP)-linked secondary antibodies were purchased from VWR. The GFER rabbit polyclonal antibody (#HPA041227 ...
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bioRxiv - Developmental Biology 2023Quote: ... using the mouse MF20 (1:500, DSHB), the rabbit anti-GFP (1:1000, Life technology) and the rabbit RFP (1:1000, VWR) primary antibodies ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: Wilt-type and ISL2 KO cells were plated in micro cover glasses (VWR cat. no. 48368-062) at a density of 2×104 in regular growth conditions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... were filled with a thin layer of 2.5% agarose substrate (VWR Life Science; type number: 97062-250). After cooling ...
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bioRxiv - Cell Biology 2024Quote: A hole was punched in the bottom of a 100 mm type 25384-302 petri plate (VWR) with a gas flame-heated 13 mm glass test tube ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1100 rpm for 16 hours using a Mini Shaking Incubator (VWR Catalog # 12620-942, VWR International LLC., Radnor, PA, USA.). 100 μL of sterile 20% glycerol was added to 100 μL of overnight culture to prepare glycerol stock plate.
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bioRxiv - Neuroscience 2020Quote: ... The olfactory epithelium was cryosectioned coronally into 16 μm -thick sections mounted on Superfrost Plus slides (VWR, West Chester, PA) coated with poly-D-lysine.
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bioRxiv - Cell Biology 2022Quote: ... HAF were seeded onto Ø 10 or 16 mm borosilicate glass cover glasses (631-0170 and 631-0151, VWR, Germany). After transfer into 12- or 24-well plates (665102 and 662160 ...
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bioRxiv - Microbiology 2022Quote: ... hexadec-9-enoic acid (P9417, MilliporeSigma), octadecanoic acid (S4751, MilliporeSigma), and pentadecanoic acid (P6125, MilliporeSigma) in ethanol (MACR6777-16, VWR), and 20% tyloxapol (T8761 ...
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bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
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bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2023Quote: ... then incubated on rollers at room temperature for 1 hour with an anti-mouse secondary antibody conjugated to horseradish peroxidase (VWR, NXA931). After four further washes for 10 min each (2 x blocking buffer ...
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bioRxiv - Immunology 2019Quote: ... or with 5×104 B16F10 wild type (WT) or B16F10-Globin-SL8-intron cells along with matrigel (VWR). Five days after challenge ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We inoculated these 16 samples in YEPD for 12 hours and extracted their genomic DNA using a commercially available kit (Amresco, Inc. VWR USA). We measured the concentration of DNA with a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoblots were then washed with TBS containing 0.05% Tween 20 (TBST) and incubated for 1 hour at ambient temperature with either anti-mouse horseradish peroxidase conjugated (NA931, VWR; 1:5000); or anti-rabbit HRP conjugated (NA934 ...
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bioRxiv - Bioengineering 2024Quote: ... AB_11125142) or an anti-mouse secondary antibody (1:3000, Cat# 170-6516, RRID: AB_11125547) and enhanced chemiluminescence reagent (VWR, Cat # 490005-018). The signal was visualized using a C-DiGit Blot Scanner (Li-Cor) ...
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bioRxiv - Developmental Biology 2021Quote: The differentiated human iPSCs were fixed on glass coverslips (VWR, Denmark) in 4% PFA for 15 min at RT and stored at 4°C until use ...
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bioRxiv - Cell Biology 2024Quote: ... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...
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bioRxiv - Cell Biology 2020Quote: ... Cryosections were cut at a thickness of 10-16 µm using a Leica CM3050 S cryostat and placed onto SuperFrost®Plus™ slides (VWR). Prior to staining ...
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bioRxiv - Immunology 2022Quote: ... region of the 16S rRNA gene was performed in 20 uL duplicate reactions: 8 uL of 2.5X 5Prime Hotstart Mastermix (VWR, Radnor, PA, USA), 1 uL of 20X Evagreen (VWR) ...
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bioRxiv - Immunology 2023Quote: ... mice were whole body perfused through the heart with 2% paraformaldehyde (diluted down in PBS from 16%; VWR Cat. No. 15710-S), then livers were harvested and placed in 2% paraformaldehyde for 24 hours at 4°C then placed in 30% sucrose (Sigma-Aldrich Cat ...
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bioRxiv - Microbiology 2020Quote: ... animals were randomly assigned to individual cages under one of four groups (n=8 per group): Wild-type (WT) C57BL/6 mice with access to a free running wheel (VWR) or a locked wheel (SED) ...
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bioRxiv - Microbiology 2024Quote: Cells from bone marrow of 6-10-week-old male or female wild-type or Myd88-/- Ticam-/- Mavs-/- (49) C57BL/6 mice (see Table S1) were cultivated in RPMI 1640 (VWR) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal (VWR GenScript A01622-40); western blot ...
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bioRxiv - Microbiology 2021Quote: ... coli 0.1229 and its derivatives were harvested after 16 h shaking at 37°C and passed through 0.2 µm cellulose acetate filters (VWR Life Sciences, Radnor, PA). Assays to quantify Stx amplification were performed as previously described (15) ...
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bioRxiv - Neuroscience 2023Quote: ... 16 µm NAcc coronal sections were obtained with a Leica CM3050S cryostat and mounted onto SuperFrost Plus glass slides (VWR, Radnor, PA, USA). The sections were collected in a way that each slide contained rostral ...
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bioRxiv - Microbiology 2021Quote: Pooled normal human plasma was obtained from Pacific Hemostasis (VWR #95059-698). Factor X and prothrombin-deficient plasma were obtained from Haematologic Technologies (#FX-ID and #FII-ID) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... each chamber was filled with 100μl food medium containing a mix of 2% agarose (VWR life science; type number: 97062-250), 2% sucrose (Merck KGaA ...
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bioRxiv - Immunology 2022Quote: ... Human colon tissue samples were fixed in 4%(w/v) PFA (VWR International) for 24h at RT ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: For egg laying small Petri dishes (4 cm diameter) were filled with a 3% agarose (VWR life science; type number: 97062-250), 3% sucrose (Merck KGaA ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human alveolar macrophages (AMs) were lysed in RIPA buffer (VWR, Radnor, PA, USA) supplemented with protease (Roche Diagnostics GmbH ...
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bioRxiv - Bioengineering 2019Quote: ... and β3TUB is a tubulin thought to be specifically involved during differentiation of neuronal cell types [69].) Antibodies were diluted in antibody dilution buffer (PBS containing 1% bovine serum albumin (VWR, Radnor, PA, USA) and 0.3% Triton X-100 (Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1 μg of either wild-type FHL-1 or RGD-null FHL-1 was incubated with 2 μg C3b (VWR International, Lutterworth, UK), and 0.4 μg factor I (FI ...