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Citations for VWR :
201 - 250 of 387 citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... slides were incubated with 0.25% Triton X for 20 min (in 1X TBST) and afterwards blocked with 2% normal donkey serum (NDS; VWR, USA) in 1X ...
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bioRxiv - Neuroscience 2022Quote: ... Cryosections were obtained using a Leica CM1860 cryostat at 20 μm thickness and adhered to Superfrost Gold Plus glass slides (VWR). For experiments requiring total internal reflection fluorescence (TIRF ...
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bioRxiv - Immunology 2023Quote: ... Cryosections were cut in OCT at 7 μm thickness on Epredia CryoStar NX50 at -20°C chamber temperature and mounted onto lysine-coated Superfrost slides (VWR). Sections were fixed for 20 min in 4% ParaFormAldehyde (PFA ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips coated with 0.1% gelatin were fixed for 20 minutes in 3% paraformaldehyde (VWR, Radnor, PA) (resuspended in PBS and brought to pH 8.0 with NaOH) ...
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bioRxiv - Microbiology 2023Quote: ... were generated by centrifugation of the grown culture at 5800 x g for 20 min and subsequent filter-sterilization (0.2 µm, VWR™). SM samples were prepared freshly before each experiment under anaerobic conditions ...
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bioRxiv - Synthetic Biology 2023Quote: ... We grew the transformed library in pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific), 5 g Casamino Acids (VWR) ...
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bioRxiv - Plant Biology 2023Quote: ... Flash chromatography was carried out using a 7 cm diameter column loaded with 20 g of P60 silica gel (SiliCycle) with hexane (HPLC grade; VWR) as the mobile phase ...
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bioRxiv - Neuroscience 2023Quote: ... Cryosections for DRG (8µm thickness) and plantar skin (20 µm thickness) sections were collected onto superfrost plus slides (VWR International). Slides were washed 3 times with PBS 0.2% Triton and then blocked with PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Embedded tissue blocks were sectioned on a cryostat in slices 10 to 20 micrometers thick and attached to Superfrost Plus microscope slides (631-0108, VWR). The sections can be stored at −80 indefinitely ...
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bioRxiv - Microbiology 2023Quote: ... Then 20 μL of hemolymph per each sample were digested with 0.5 mL of 32% ultrapure hydrochloric acid (VWR Chemicals) under heating conditions (60 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... we picked individual colonies into 4 mL of pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter -20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific), 5 g Casamino Acids (VWR) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Washing was carried out three times for 10 min at room temperature with TBS + 0.1% Tween 20 (VWR, United States) and the membrane was incubated with appropriate fluorescent secondary antibodies (listed in Supplementary Table S1 ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int. LLC, Cat# 23486.297, and glycerine, VWR, Cat# 24387.292) in a refrigerator until sectioning ...
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bioRxiv - Biophysics 2019Quote: ... After washing and collection by centrifugation crude peptides were dissolved in 20% (v/v) acetonitrile (ACN, Jencons-VWR, Leicestershire, United Kingdom) / 80% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... followed by cryoprotection in graded sucrose solutions (10 %, 20 %, 30 %) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 μm thick ...
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bioRxiv - Microbiology 2020Quote: ... Two-µl spots of each dilution were plated on selective (20 µg/mL thiamphenicol) and unselective square (90×15 mm, VWR international) BHI plates with or without 200 ng/mL anhydrotetracycline (ATc) ...
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bioRxiv - Developmental Biology 2020Quote: ... 10% formamide and 2x saline sodium citrate (SSC) supplemented fresh each experiment with 0.1% Tween-20 and 2 μg/mL nuclease-free BSA (VWR, 0332-25G)) ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was blocked with TBS-T (20mM Tris-HCl, pH 7.6, 136mM NaCl and 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cryoprotection in graded sucrose solutions (10%, 20%, 30%) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 µm thick ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was centrifuged at 14000×g for 40 min and a Macrosep advanced filter (3000 Da MW cutoff, 20 ml; catalogue no. 89131-974; VWR, USA) was used to remove the free biotin ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii cultures were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
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bioRxiv - Neuroscience 2023Quote: Tissue for HCR was cryosectioned at 30 μm at -20°C taking coronal sections that were mounted on Superfrost® slides (VWR). The mounted slides were allowed to dry for 24hrs at room temperature before being stored at -80°C before HCR ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 mL LB or BHI medium was inoculated with 20 µL preculture and grown in a 24-well plate (VWR, USA). Furthermore ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii strains were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
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bioRxiv - Neuroscience 2023Quote: ... and 10 – 20 mosquitoes were sorted into a behavioral container made from a 32-ounce HDPE plastic cup (VWR #89009-668). The behavioral container was prepared by cutting a 10 cm hole in the lid with a razor blade and covering the cup with a 20 cm x 20 cm piece of white 0.8 mm polyester mosquito netting (American Home & Habit Inc ...
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bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
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bioRxiv - Cell Biology 2019Quote: ... Fetal liver was dissected from the embryo and embedded in a 25-mm x 20-mm x 5-mm Tissue-Tek Cryomold (VWR, 25608-916) with optimal cutting temperature compound (Tissue-Tek O.C.T. ...
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bioRxiv - Neuroscience 2019Quote: ... Our CAFÉ assay consisted of a 6-well plate with 4 small holes drilled for the insertion of pipette tips and 20 µl capillaries (VWR, Radnor, PA). Capillaries were filled via capillary action ...
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bioRxiv - Genetics 2020Quote: ... Transformants were pooled and cultured in low-pH Sabouraud Dextrose Casamino Acid media (SDCAA, per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific, Radnor, PA), 5 g Casamino Acids (VWR) ...
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bioRxiv - Microbiology 2020Quote: Microspheres were washed once with deionized water and incubated for 20 minutes in an end-over-end rotator in the dark in a suspension of 0.1 M NaH2PO4 (VWR; Radnor, PA, USA), 5 mg/mL EDC (1-Ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Immunology 2022Quote: ... region of the 16S rRNA gene was performed in 20 uL duplicate reactions: 8 uL of 2.5X 5Prime Hotstart Mastermix (VWR, Radnor, PA, USA), 1 uL of 20X Evagreen (VWR) ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue was cut on a cryostat (Microm HM500-CM) at a thickness of 20 µm and mounted on glass slides (Superfrost Plus, VWR, 48311-703). Fluorescent in-situ hybridization was performed according to the ACD-Bio RNAscope Multiplex Assay manual ...
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bioRxiv - Immunology 2020Quote: ... Microspheres were washed once with deionized water and incubated for 20 minutes in the dark in an end-over-end rotator suspension of 0.1 M NaH2PO4 (VWR; Radnor, PA, USA), 5 mg/mL EDC (1-Ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Neuroscience 2021Quote: ... Cryosections were obtained using a Leica cryostat set at 20-30 μm and mounted on Superfrost Plus Micro slides (VWR, 48311-703). On the day of staining ...
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bioRxiv - Biochemistry 2022Quote: ... Individual yeast colonies were picked into 4mL of pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific, Radnor, PA), 5 g Casamino Acids (VWR) ...
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bioRxiv - Biophysics 2022Quote: ... peptides were resuspended in 20 μL of resuspension buffer (5:95 ACN:MilliQ H2O, 0.1% TFA) and solubilized in a bath sonicator (VWR, 35 kHz, 10 min). A ZipTip was placed on a P10 pipettor set to 10 μL ...
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bioRxiv - Bioengineering 2023Quote: ... the strips were immersed in a blocking buffer (1% BSA and 0.05% Tween 20) for one hour on an orbital shaker (no. 57018-754VWR; VWR International, Brisbane, CA) set at 150 rpm and thereafter dried overnight at 4° Celsius ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmid DNA was quantitated using a NanoDrop spectrophotometer and diluted to 20 fmol/μL in nuclease-free water (VWR 02-0201-0500). An appropriate volume of reaction mastermix was prepared with the following composition ...
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bioRxiv - Neuroscience 2023Quote: ... The PVDF membranes were blocked in 5% Nonfat dry milk (Bio- Rad Laboratories, Cat.# 1706404) in Tris-buffered saline with 0.1% Tween 20 (TBST) (VWR, Cat.# K873-4L). Samples were probed with the primary antibodies listed in Supplemental Table 1 overnight in 5% bovine serum albumin (Gold Biotechnology ...
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bioRxiv - Microbiology 2023Quote: ... 1% agar [Teknova, A7777] containing 40 µg/mL Congo red dye [VWR, AAAB24310-14] and 20 µg/mL Coomassie blue dye [VWR, EM-3300]) in a 10 cm X 10 cm X 1.5 cm plates ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were placed in a Leica cryostat one hour prior to sectioning and sectioned at 20 μm onto Superfrost plus microslides (VWR, 48311-703). Samples were stored in -80 °C until use.
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bioRxiv - Cancer Biology 2023Quote: ... we let the frozen slides reach –20 °C before transferring them directly to 1X PBS/4% paraformaldehyde (PFA) (VWR, cat. no. 10047105). We incubated the sections in PFA for 10 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were sliced into 1mm thick coronal slices at -20°C using a metal brain matrix and .22mm razor blades (Ted Pella, 15045; VWR, 55411-050) and were then placed on dry ice and covered to prevent condensation ...
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bioRxiv - Molecular Biology 2021Quote: ... or 5% (w/v) non-fat dry milk in TRIS-buffered saline (TBS) containing 0.1% Tween-20 (TBS-T; VWR, Radnor, PA, USA, # 28829.183) for one hour at room temperature and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads are removed from glass and resuspended in TE-TW (TE buffer: Sigma-Aldrich, Inc., 8910-1L; Tween-20: VWR International, LLC, 100216-360) and subjected to 2 TE-TW washes followed by centrifugation for 2min at 3000rcf ...
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bioRxiv - Neuroscience 2023Quote: ... and stored at −20 °C in an anti-freeze solution (30 % glycerol [Merck Millipore, Germany], 30 % ethylene glycol [VWR International, USA] and 10 % TBS) until further processing ...
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bioRxiv - Synthetic Biology 2023Quote: ... an overnight LB culture of the indicated strain and expression plasmid was used to inoculate 20 mL of AB autoinduction media with the appropriate antibiotics in a vented baffled 250mL Erlenmeyer flasks (VWR, cat. no. 89095-270). Flasks were incubated at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Retinal slices were obtained by cryosectioning the eyes at 20 µm thickness and mounting on Superfrost® Plus Micro Slide (VWR, Cat#48311-703). The sections were either stained immediately or stored at −80°C.