Labshake search
Citations for VWR :
201 - 250 of 283 citations for L Phenylalanine N T Boc 13C9 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... and cultured in MEM (with Earle’s salts, without L-glutamine, with NEAA; VWR # L0430-500) media with 15% FBS and 1% pen-strep ...
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bioRxiv - Microbiology 2023Quote: ... spread onto LB agar plates with an L-shaped cell spreader (VWR# 220412261B, PA, USA), and incubated statically overnight at 37°C.
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bioRxiv - Developmental Biology 2024Quote: ... 0.1mmol/l ß-mercaptoethanol and 15% (v/v) FBS (HyClone HYCLSH30088.03, VWR and SH30071.03, Cytiva) (M15Hy) ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed 3x for 15 minutes with PBS-T (PBS with 0.02% Tween) and incubated for 2 hours with either anti-rabbit HRP-linked (VWR, cat. no NA934) or anti-mouse HRP-linked (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... The normalized libraries in a pool with a total of 311 µl libraries were incubated with 77 µl of 0.2 N Sodium Hydroxyl (NaOH) (VWR, 82023-092) at room temperature for 8 minutes to denature double-stranded DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were diluted with ultrapure water to a final concentration of 0.475 N nitric acid and transferred to metal-free centrifuge vials (VWR, 89049-172) for subsequent ICP-MS analyses.
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bioRxiv - Cell Biology 2021Quote: ... fixed platelet subpopulations were centrifuged onto poly-l-lysine coverslips (VWR; 600 x g, 5 minutes), permeabilized with 0.2% Triton ...
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bioRxiv - Evolutionary Biology 2022Quote: ... grown to saturation in liquid SDCAA (6.7 g/L YNB without amino acid VWR #90004-150), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...
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bioRxiv - Genomics 2022Quote: ... using an 80 mbar Welch WOB-L® Dry Vacuum Pump (VWR International; Mississauga; ON Canada). We filtered the three 50 mL microbial mock community mixtures and added a negative filtration control by additionally filtering 50 mL of the ultrapure water that was used to set up the mixtures ...
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bioRxiv - Microbiology 2019Quote: ... polymyxin B Sulfate (Fisher catalog# 52-915-GM) and poly-L-lysine hydrochloride (VWR catalog# IC15269080). All chemicals were dissolved in ultra-pure DI water followed by filter-sterilization ...
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bioRxiv - Microbiology 2023Quote: ... in tryptic soy broth (TSB, Difco) or in M9 minimal medium (KH2PO4 3.4 g/l, VWR; K2HPO4 2.9 g/l ...
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bioRxiv - Microbiology 2024Quote: ... the mice were provided with water supplemented with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) for two days ...
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bioRxiv - Molecular Biology 2021Quote: ... or 5% (w/v) non-fat dry milk in TRIS-buffered saline (TBS) containing 0.1% Tween-20 (TBS-T; VWR, Radnor, PA, USA, # 28829.183) for one hour at room temperature and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: 5×106 MM.1Sgfp+/luc+ cells were treated with DMSO or 1.00 μM TriC for 24 h in T-25 flasks (Avantor/VWR; Cat. no. 10861-568). Cells were then harvested ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-2 preps of in vitro transcribed RNA and 5-10µg N gene RNA were mixed with the prepared cell suspension in a 4mm electroporation cuvette (VWR International # 89047-210) and pulsed in a BioRad GenePusler using the exponential protocol with the following settings ...
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled Rabbit Anti-Sheep IgG (H+L) secondary antibody (VWR, Batavia, IL, USA) diluted 1:1000 (100ng/mL ...
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bioRxiv - Cell Biology 2023Quote: Hippocampal neurons seeded at 180,000 cells/well onto 0.1 mg/mL poly-L-lysine coated coverslips (#1.5, VWR) in 6-well plates ...
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bioRxiv - Microbiology 2021Quote: ... Vero cells were seeded at 60% confluence in 24-well plates equipped with 13 mm round glass coverslips (VWR, cat n. 631-0150). Cells were washed once with PBS before incubation with BetaCoV/Australia/VIC01/2020 diluted in PBS at an MOI=5 ...
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bioRxiv - Microbiology 2023Quote: ... infectivity and spread cells were stained with a SARS-CoV-2 anti-N rabbit polyclonal antibody for 1 hour followed by 594-conjugated secondary Goat anti-Rabbit IgG (VWR, GtxRb-003-D594NHSX) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... transfected and infected cells were stained with an anti-N rabbit polyclonal antibody followed by incubation with secondary 594-conjugated Goat anti-Rabbit IgG (VWR, GtxRb-003-D594NHSX). Nuclei were stained with DAPI (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Starvation medium contained 1,7g/L Yeast Nitrogen Base without amino acids and ammonium sulfate (#J630-100G, VWR, USA) and 2% glucose (#X997.5 ...
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bioRxiv - Bioengineering 2020Quote: ... and buffer-exchanged to lower conductivity sucrose-dextrose dielectrophoresis buffer (DEP buffer: 2.39 g/L HEPES (VWR 3638C017), 80.7 g/L sucrose (Sigma-Aldrich S0389) ...
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bioRxiv - Physiology 2021Quote: ... Cryostat-cut sections (8-10μm) were mounted directly onto poly-L-lysine-covered glass slides (VWR, Leuven, Belgium) by the Institute of Metabolic Science Histology Core ...
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bioRxiv - Synthetic Biology 2021Quote: ... HEK293T and HEK293TERK2-EGFP cells were cultured in DMEM media supplemented with high glucose and L-glutamine (VWR) supplemented with 10% Hyclone FetalClone I serum (VWR ...
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bioRxiv - Plant Biology 2020Quote: ... USA) and then moved into a forced-air oven (VWR Oven F Air 104 L, VWR, Radnor, PA) at 65 °C to dehydrate ...
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bioRxiv - Genetics 2020Quote: ... nos-Cas9/nos-Cas9 (Fig. 4A) males in 0.3 L plastic bottles (VWR® Drosophila Bottle 75813-110). Parent (P ...
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bioRxiv - Microbiology 2019Quote: ... Tissue cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, (VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
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bioRxiv - Microbiology 2022Quote: ... gondii co-cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
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bioRxiv - Microbiology 2023Quote: ... The end of the Tongue Cleaner was placed in a sterile vessel containing 2mL phosphate-buffered saline (PBS, pH=7.4, M/L= 1.37M sodium chloride, 0.27M potassium chloride, 0.1M phosphate buffer; VWR, Lutterworth, UK) and 1g of 3.5-5.5mm sterile glass beads (VWR ...
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bioRxiv - Cell Biology 2023Quote: ... Embryonic hippocampi were dissociated using papain and seeded at 180,000 cells/well for microscopy experiments on 0.1 mg/mL poly-L-lysine coated wells or glass coverslips (#1.5, VWR) in 6-well plates ...
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bioRxiv - Bioengineering 2024Quote: ... Chondrocytes were cultured in Dulbecco’s modified Eagle’s medium (DMEM, no glucose, L-Glutamine, and sodium pyruvate; VWR, 11966025) with 10% fetal bovine serum (R&D Systems ...
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bioRxiv - Genomics 2020Quote: ... 13.4 g/ l (1.34% (w/v)) Yeast Nitrogen Base (Sunrise Science #1501-500) and 2% glycerol (VWR #AA36646-K7).
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bioRxiv - Bioengineering 2020Quote: ... then cells were homogenized and plated in B27-containing Neurobasal medium on Poly-L-Lysine treated #1.5H glass coverslips (Marienfeld, VWR) to a density of 4000 cells per cm2 ...
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bioRxiv - Immunology 2021Quote: ... containing amphibian-adjusted Leibovitz’s L-15 (AL-15; Wisent Inc.) medium supplemented with 15% fetal bovine serum (FBS; VWR), herein referred to as Xela complete media ...
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled Rabbit Anti-Deer IgG (H+L) secondary antibody (95058-328, VWR, Batavia, IL, USA) diluted 1:1000 (100ng/mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150] ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Tryptic peptides were resolubilized in 30 μl of 0.05% TFA and 2% ACN in H2O filtered through 10 kDa MWCO PES membrane spin filters (VWR) at 14,000×g for 15 min and transferred to HPLC vials.
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bioRxiv - Synthetic Biology 2023Quote: ... 10 mL of seed culture was used to inoculate 1 L media in a Fernbach flask (VWR 29171-854). Cells were incubated at 37 °C shaking at 210 rev/min ...
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bioRxiv - Microbiology 2023Quote: ... Sorbarod filter samples were homogenised via placement in half-strength thioglycolate medium (14.5 g/L; Oxoid, Basingstoke, UK) and 1g of 3.5-5.5mm sterile glass beads (VWR, Lutterworth, UK). Samples were vortexed (30 seconds) ...
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bioRxiv - Microbiology 2024Quote: ... blood was collected by cardiac puncture into incomplete RPMI with 25mM HEPES and L-Glutamine (VWR Cat# 45000-412) at 37°C and enriched by an Accudenz discontinuous gradient as described above ...
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bioRxiv - Microbiology 2023Quote: The pH measurement of all samples was performed using a pH electrode (VWR® PHenomenal® PH 1000 L). Before performing the measurement ...
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bioRxiv - Microbiology 2024Quote: Bacterial load in faeces was determined using approximately 500 µL of frozen faecal slurry (238 – 816 mg) and diluting it 400,000 times in physiological solution (8.5 g/L NaCl; VWR International). Next ...
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bioRxiv - Bioengineering 2021Quote: Fresh frozen lung lobe tissues with highest injury score (n=9) were homogenized using VWR® Mini Bead Mill Homogenizer (VWR International LLC., Radnor, PA) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in normal growth medium then switched to cysteine- and methionine-free medium DMEM supplemented with 2 mM L-glutamine (VWR) and 100 µM HPG (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... 50 µL of the collagen-I mixture was pipetted into the bottom of the wells of an 8-chamber slides (VWR) to coat the surface and allowed to gel for 20 minutes at 37°C ...
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bioRxiv - Microbiology 2021Quote: Gametocytes were produced by treatment of the mice at 1% parasitemia with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) in their drinking water for two days before exsanguination ...
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bioRxiv - Cell Biology 2021Quote: ... 20 μl of cells were put to 180 μl of TYI-S-33 media in 96-wells Tissue Culture Plate (VWR) and the same dilution step was performed 5 times ...
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bioRxiv - Cell Biology 2022Quote: ... The integrated vector backbone was removed by growth in non-selective YT media (5 g/l yeast extract (VWR, EM1.03753.0500), 5 g/l tryptone (Fisher Scientific ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... 200 µl of this resuspended culture was aliquoted into a 96-well black/clear glass bottom sensoplate (Greiner Bio-one VWR). Four technical replicas were measured per culture ...
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bioRxiv - Evolutionary Biology 2022Quote: ... yeast cultures were diluted to OD600=0.67 in 5 mL SGDCAA (6.7 g/L YNB without amino acid VWR #90004-150), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...