Labshake search
Citations for VWR :
251 - 300 of 422 citations for Hyaluronidase PH 20 SPAM1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Food rewards were 20 mg pellets (Dustless Precision Pellets Rodent Grain-Based Diet; VWR 89067-546) delivered to a trough ...
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bioRxiv - Microbiology 2022Quote: ... 200 μL of each overnight culture was resuspended in 20 mL of Marine Broth 2216 (VWR) in an Erlenmeyer flask in shaking incubator at 220 rpm for 3 h until each species reached an optical density of 0.15 ...
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bioRxiv - Microbiology 2022Quote: ... in PBS supplemented with 0.1 % Tween-20 (PBST) containing 5 % (w/v) skim milk powder (VWR). The membranes were then incubated for 1 h with primary antibodies diluted 1/3,000 in 5 % milk PBST ...
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bioRxiv - Neuroscience 2022Quote: ... Food rewards were 20 mg pellets (Dustless Precision Pellets Rodent Grain-Based Diet; VWR 89067-546) delivered to a trough ...
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bioRxiv - Microbiology 2022Quote: ... and supplemented with 10% sheep defibrinated blood (Hemostat) with 20 μg/mL streptomycin (VWR Life sciences) or classical LB broth (Difco ...
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bioRxiv - Microbiology 2023Quote: ... in PBS supplemented with 0.1 % Tween-20 (PBST) containing 5 % (w/v) skim milk powder (VWR). The membranes were then incubated for 1 h with primary antibodies diluted in PBST with or without 5 % milk ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1% BSA) and disrupted with 20 strokes in a homogenizer containing a pure PTFE head (VWR International) and two strokes with a dounce tissue grinder with tight-fitting pestle (DWK Life Sciences Kimble Kontes) ...
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bioRxiv - Cell Biology 2020Quote: ... except cells were disrupted with 20 strokes in a homogenizer containing a pure PTFE head (VWR International) and two strokes with a dounce tissue grinder with tight-fitting pestle (DWK Life Sciences Kimble Kontes) ...
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bioRxiv - Neuroscience 2022Quote: ... The blocks were sectioned to 20 µm with a cryostat microtome and mounted on microscope slides (VWR). The sections were stored at -20 °C until needed for immunofluorescence ...
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bioRxiv - Neuroscience 2022Quote: ... single-housed rats were given 24h concurrent access to one bottle of 20% (v/v) alcohol (VWR International S.A.S. ...
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bioRxiv - Physiology 2022Quote: ... Samples were kept at -20°C throughout the extractions in an Iso-Therm System (VWR #20901-646). Fifteen milligrams of liver were homogenized in 200 µL of a 3:3:2 solution of acetonitrile:isopropanol:water (MeCN:IPA:H2O ...
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bioRxiv - Microbiology 2023Quote: ... Filters were immediately transferred into a 40:40:20 (v-%) acetonitrile (Honeywell # 14261-1l)/methanol (VWR # 83638.320)/water extraction solution at -20°C ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to nitrocellulose overnight in 20% methanol in Tris/Glycine transfer buffer (VWR, #97061-382). Total protein was visualized using the LI-COR REVERT™ Total Protein Stain (Licor ...
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bioRxiv - Molecular Biology 2024Quote: ... secondary antibody (goat-anti-mouse-Cy3: VWR-95040-042 or goat-anti-rabbit-cy5 or Donkey anti-chicken-488 ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Immunology 2019Quote: ... 20 µm coronal sections were cut on a cryostat and thaw-mounted onto Superfrost Plus slides (VWR Scientific). Sections were collected in a 1:6 series from the diagonal band of Broca (bregma 0.50 mm ...
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bioRxiv - Microbiology 2021Quote: ... and 20 mL of this mixture were poured into a 150 mm diameter petri dish (VWR, Radnor, PA) and cured at 70° C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... 2900 rpm 10 min and 3500 20 min) and passed through a 0.22 µm PES membrane filter (VWR). To enrich EV concentration we used the Total Exosome Isolation kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... purified cells were diluted and imaged at 100x magnification within a 20 μm counting chamber (VWR 15170-048). Images were analyzed using FIJI/ImageJ 1.52p (Java 1.8.0_172 ...
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bioRxiv - Biochemistry 2019Quote: ... The dissociated brain tissue samples were gently homogenized (20 strokes) with a glass-Teflon homogenizer (# 89026-384 VWR). The homogenized tissue samples were filtered with 40 μm mesh filter (# 22-363-547 Fisher scientific) ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... cells were scraped into 1mL fresh KA medium using disposable cell scrapers with 20 mm blade width (VWR). Cell pellets were washed once and then resuspended in 110 µl of fresh KA medium ...
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bioRxiv - Neuroscience 2023Quote: ... The OCT embedded spheroids were cryo-sectioned at 20 µm and mounted on positively charged Superfrost slides (VWR). Slides can be stored at −80°C until further use.
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bioRxiv - Neuroscience 2023Quote: ... Tissues were then sectioned on a Leica cryostat at 20 μm onto Superfrost plus microslides (VWR, 48311-703) and stored at −80 °C until use.
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bioRxiv - Cell Biology 2023Quote: ... The tissue blocks were cryosectioned (20 µm) and mounted on microscope slides (Superfrost Plus Adhesion Microscope slides, VWR) and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... The samples were centrifuged at 17,000 × g for 20 min and supernatants were sterilized using 0.2 µm cellulose acetate membrane filters (VWR) and kept at −20 °C until further analysis ...
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bioRxiv - Microbiology 2020Quote: ... were cut with a cryostat and 3 sections of 20 µm were placed on each microscope glass slide (VWR). Sections were fixed with acetone for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... the ratio 9:1 of ATL buffer to Proteinase K was kept but the volume was increased to 720 ATL and 80 Proteinase K and an incubation step of 37 °C for 15 min was added after the addition of 50 μl of EBT (EB buffer with 0.05% Tween-20 (VWR)) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the samples were vortexed 4 × 20 seconds at full speed on a vortex Genie 2 (VWR Scientific Products). The cellular debris were removed by centrifugation at 2,600g for 5 min at 4°C and 1.5 ml of the lysate was transferred to a micro-tube and centrifuged again at 9500 g for 5 min at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: Samples were cryosectioned using CM3050S Leica cryostat at 16μm (nose) and 20 μm (brain) collected on Superfrost Plus Micro Slides (VWR). All sections were stored at −80°C until ready for staining or in situ hybridization.
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bioRxiv - Synthetic Biology 2021Quote: ... and then cell suspensions were added with 20% (w/v) glass beads (0.5 mm) and the cells were grinded with hand-powered electrical motor (VWR). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... All washing steps between blocking or chemical incubation were performed with PBS supplemented with 0.5 % Tween-20 (VWR, 8.22184).
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bioRxiv - Systems Biology 2020Quote: ... Protein and crosslink digestion was carried out by adding 50 μL freshly prepared 20 mg/mL Proteinase K (VWR, freeze-dried powder suspended in 10 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... filtered through a Chromafil® GF/PET-20/25 filter (pore size 1.0 and 0.2 μm, VWR, Vienna, Austria) and stored at –20°C until analysis ...
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bioRxiv - Microbiology 2021Quote: ... Free biotin was removed by Macrosep Advance Spin Filter 3K MWCO 20 mL (Cat no. 89131-974, VWR, USA). Aliquots of the samples were stored at −80°C for use as input in western blot ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 µm sections were cut from the OCT blocks and mounted on positively charged slides (VWR superfrost Plus slides). The slides were then stored at -80° C until use.
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR, 0777), and incubated overnight with the following primary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... cells were scraped into 1 mL fresh KA medium using disposable cell scrapers with 20 mm blade width (VWR). Cells were pelleted with the respective supernatants discarded and then flash frozen in a dry ice-ethanol bath to preserve RNA integrity and stored at −80°C ...
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bioRxiv - Systems Biology 2023Quote: ... In the final steps the beads were resuspended in 20 μL 0.1 % formic acid in water and sonicated in an Ultrasonic Cleaner USC-T (VWR) for 10 min and the supernatant was transferred to a new plate ...
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bioRxiv - Neuroscience 2023Quote: ... 1:10,000 NaOH 10N, 0.02% sodium azide) for 1 hour for each step (20%, 40%, 60%, 80%) on a nutator (VWR). Samples were then washed with 100% MeOH 2x for 1hr each and then incubated in a 2:1 dicholoromethane (DCM):MeOH solution overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Whole pancreas or thick sections were dehydrated in a methanol/PBS series (20%, 40%, 60%, 80%, 100% methanol, diluted in PBS; 20847.295, VWR), 1 h (whole pancreas ...
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bioRxiv - Microbiology 2023Quote: ... 8% CO2 with continuous shaking and culture supernatant was then clarified by centrifugation at 4000 x g for 20 minutes and filtered using 0.2 μm vacuum filtering system (VWR). SOSIP glycoproteins were purified from the supernatant by affinity chromatography and further by size exclusion chromatography as follows ...
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bioRxiv - Microbiology 2023Quote: ... afra leaf material was ground and extracted in dichloromethane (DCM) (1 g:20 mL, VWR International, Radnor, PA, USA) under sonication for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... the developing hBOs were grown in neural induction media supplemented with 20 ng/ml BDNF (VWR: RL009-001-C27) and 20 ng/ml NT-3 (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... were transferred to a 5 mL or 10 mL round bottom flasks manufactured from borosilicate 3.3 glass with a 14/20 Joint size (VWR) and dried to a film by streaming N2 ...
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bioRxiv - Immunology 2021Quote: ... 50 μL of mAbs diluted to 20 μg/mL in PBS were added to the first well of a 96-well U-bottom plate (VWR), and diluted two-fold in PBS for 25 μL mAb total per dilution ...
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bioRxiv - Genetics 2020Quote: ... flies were anesthetized under light CO2 and groups of five flies were placed in individual scintillation vials (VWR 74504-20). To extract CHCs ...
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bioRxiv - Neuroscience 2021Quote: ... Cryosections were obtained using a Leica CM1860 cryostat at 20 μm thickness and adhered to Superfrost Gold Plus glass slides (VWR).
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bioRxiv - Physiology 2020Quote: Frozen brains were cryosectioned at 20 µm and sections containing PT and MBH were mounted to pre coated adhesion slides (SuperFrost Plus, VWR). Brain sections were fixed in 4 % PFA (0.1 M PB ...
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bioRxiv - Bioengineering 2020Quote: ... microgels were washed with 500 μL HFE 7500 and 100 μL 20% v/v 1H,1H,2H,2H-Perflourooctonal (PFO) (VWR) in HFE for each 125 μL of microgels multiple times to remove the surfactant ...