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Citations for VWR :
251 - 300 of 1037 citations for Human Immunodeficiency Virus P24 Protein HIV 1 Clade C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... in Ce3D using Frame Seal incubation chambers (Bio Rad) and kept at 4°C in the dark after coverslipping (thickness no. 1.5; VWR). Slices were imaged on a Zeiss LSM 880 confocal microscope using a 32 channel Gallium arsenide phosphide (GaAsP ...
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bioRxiv - Cell Biology 2020Quote: ... After 1h binding on a wheel at 4°C beads were washed 3x in PBS supplemented with 0.02% Tween20 (VWR, M147) and 1% SDS and eluted with NuPAGE LDS buffer supplemented with 50 mM DTT and 1 mM biotin at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were centrifuged at 14000 rpm at 4°C and the supernatants clarified with a 0.2 μm membrane syringe filter (VWR International) before proceeding with a two-step purification protocol ...
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bioRxiv - Synthetic Biology 2022Quote: Single colonies were picked and cultured for 24 hours at 30 °C in 3 mL YPD liquid medium supplemented with appropriate antibiotics in 14 mL culture tubes (VWR) as seed culture ...
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bioRxiv - Plant Biology 2021Quote: ... was kept at 30°C during the injections and all eluents were filtered through 0.2 μm PES filter (VWR, USA) and stored under helium ...
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bioRxiv - Immunology 2020Quote: ... or Fc.IL-2 for 15 min at 37°C in complete RPMI (RPMI (Hyclone) supplemented with 10% FBS (heat inactivated, VWR), L-glutamine ...
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bioRxiv - Plant Biology 2021Quote: ... was dried in a forced-air oven at 37.7 ° C for 48 hours before being weighed on a precision balance (VWR 164AC).
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bioRxiv - Biophysics 2020Quote: ... The cell lysate was separated from insoluble material by centrifugation at 40,000 g for 60 minutes at 4 °C and subsequently incubated with a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C on a rotator for affinity chromatography via the 6xHis-tag ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Flies were then returned to the fridge at 11 °C in individual containers (OD: 33mm, 60 ml, VWR international, USA) with 50 µl of distilled water on piece of filter paper.
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bioRxiv - Biochemistry 2019Quote: ... microalgae were harvested via centrifugation at 13G (9000 rpm) for 20 mins 4°C using sterile 250 ml centrifuge tubes (VWR) and concentrated in a 50 ml sterile tubes (VWR ...
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bioRxiv - Microbiology 2020Quote: ... Culture flasks were placed in a Grant Instruments Sub Aqua Pro dual water bath at 37 °C and agitated by stirring with a 12 mm magnetic stir bar (VWR), coupled to a magnetic stir plate (2mag MIXdrive 1 Eco and MIXcontrol 20 ...
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bioRxiv - Cell Biology 2021Quote: ... After solidification of the gelatin block for 6h at 4°C it was embedded in a larger OCT block (Qpath, VWR) and subjected to cryosectioning (10 µm ...
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bioRxiv - Bioengineering 2020Quote: ... Tissues were either flash frozen and stored at -80°C until further use or immediately embedded in the Optimal Cutting Temperature compound (OCT, VWR) and stored at -20°C until immunohistochemical staining ...
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bioRxiv - Developmental Biology 2021Quote: Paraffin embedded brain sections were deparaffinized for 45 min at 60°C in the oven and 2x 10 min in Xylene (VWR). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... were washed with 5% HellmanexIII (Lactan, 105513203) in ≥18 MΩ MonoQ H2O at 65°C in a table top Incu-Line oven (VWR) for 4 h and then rinsed 10 times with ≥ 18 MΩ MonoQ H2O ...
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bioRxiv - Bioengineering 2022Quote: ... then incubated for 72 hours at 37°C in 5% CO2 humidified atmosphere with gas permeable plate sealing film (VWR). All wells had a DMSO concentration of 0.5% v/v with drugs MRX-2843 (25 – 800 nM) ...
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bioRxiv - Genomics 2022Quote: ... multimodal slides with leaf sections attached to the capture areas were incubated for 2 minutes at 37°C followed by a 40-minute fixation in methanol (VWR) at -20°C ...
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bioRxiv - Microbiology 2022Quote: ... albicans were cultured at 37 °C with aeration in 5 mL of YPD (10g/L Yeast Extract (VWR J850-500G), 20g/L Peptone (VWR J636-500G) ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 minutes prior to incubating for 72 hours at 37°C in a 5% CO2 humidified atmosphere with gas permeable plate sealing film (VWR). Following incubation ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Jag1-Fc was immobilized by overnight incubation at 4°C in individual wells of non-tissue culture-treated 6 or 12 well plates (VWR) at a final concentration of 2 µg/mL in DPBS containing 10 µg/mL poly-D-lysine (ThermoFisher Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Blood plasma was obtained by centrifuging at 5000 rpm for 10 minutes at 4 °C and stored in cryotube vials (VWR Micro-centrifuge tubes with graduation 2.0 mL ...
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bioRxiv - Immunology 2023Quote: ... Cryosections were cut in OCT at 7 μm thickness on Epredia CryoStar NX50 at -20°C chamber temperature and mounted onto lysine-coated Superfrost slides (VWR). Sections were fixed for 20 min in 4% ParaFormAldehyde (PFA ...
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Tracer-based metabolomics for profiling nitric oxide metabolites in a 3D microvessel-on-a-chip modelbioRxiv - Cell Biology 2023Quote: ... The samples were then kept at 55°C for 30 minutes in a shaker (Incubating microplate shaker, VWR, The Netherlands), 20% of formic acid (5 µL ...
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bioRxiv - Biochemistry 2023Quote: ... pH 9) was performed for 30 min using a water bath at 95 °C in a coplin jar (VWR Chemicals). Spheroid sections were blocked with Tissue Blocking Buffer (2% [v/v] normal mouse serum and 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... 4 °C) and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals ...
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bioRxiv - Microbiology 2023Quote: ... aureus cells were grown at 37 °C with aeration in liquid tryptic soy broth (TSB; Difco) or on solid tryptic soy agar (TSA; VWR) medium ...
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bioRxiv - Pathology 2024Quote: ... Reverse transcription was performed for 30 min at 42 °C using dNTP Mix (10 mM each, VWR, Radnor, PA, USA) and SuperScript II Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... were then exposed to cold treatments of -15°C for up to 48 hours (in increments of 4, 8, 12, or 48 hours) in a cooling circulator (VWR Scientific 1167P Heating/Cooling Recirculating Water Bath ...
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bioRxiv - Plant Biology 2020Quote: 1-Aminocyclopropane-1-carboxylic acid (ACC; VWR P10007) was dissolved in sterile water (10 mM stock ...
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 (VWR) were cleaned by a 20 minute exposure in a 2.6 L Zepto plasma laboratory unit (Diener Electronic) ...
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bioRxiv - Cancer Biology 2023Quote: ... and Matrigel Basement Membrane Matrix (1:1) (354234, VWR).
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bioRxiv - Biophysics 2020Quote: ... The lipid mixture was suspended in 1 ml PBS at room temperature (POPC) or 55 °C (DPPC) and then sonicated for 10 minutes in an ultrasound bath (USC500TH, VWR, England) at the same temperature to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel containing organoid samples were removed from multi-well tissue culture dishes (using a 3.5 mL disposable transfer pipette and placed in 45 mL of cold PBS (4°C) in 50 mL capacity polypropylene centrifuge tubes (VWR Scientific). Matrigel was washed away by 3-4 rounds of suspension in cold PBS and centrifugation using a refrigerated centrifuge set to 1,000 pm ...
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bioRxiv - Microbiology 2020Quote: All strains were cultured for 10 hours with aeration at 37°C in brain heart infusion (BHI; VWR, Radnor, PA, USA) medium ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted at 5,000 rpm for 15 minutes at 4°C and resuspended in 10 mL of lysis buffer [300 mM NaCl (VWR BDH9286), 20mM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2021Quote: ... respectively) at 101 °C at atmospheric pressure using a temperature-controlled magnetic hotplate stirrer with a ceramic plate (VWR, Langenfeld, Germany). The PCA concentration was measured using the HPLC analytics protocol described above.The pH was measured with a VWR pH-electrode pHenomenal® 110 (Langenfeld ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 and heated for 2 hrs at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001), (3 ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to an empty tube and heated for 2 hr at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001).
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 (prepared from trace metal grade HNO3) and heated for 2 hrs at 90°C in a loosely capped 15 ml centrifuge tube (metal free, VWR, #490001), (2 ...
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bioRxiv - Immunology 2021Quote: ... 2mM l-glutamine and 25mM HEPES) were incubated for 96 hours at 37°C either on a 360° rotator (VWR International) or standing stationary (as specified) ...
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bioRxiv - Cancer Biology 2019Quote: ... O/N at 4°C before processing for paraffin embedding and the other half was embedded in Optimun Cutting Temperature compound (VWR chemicals). At least 2 serial sections of 5 μm were stained with ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... After “bubbling,” each resulting fluorogenic dye sample was placed in a 37 °C degree water bath (VWR 1228 Digital Water Bath) for fifteen minutes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lid locks were placed on each tube and incubated at 115°C for 30 min (VWR model 96 place heating block). Samples were dried in a centrifugal evaporator (SpeedVac ...
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bioRxiv - Microbiology 2023Quote: ... all 1123 presumptive Bacillus isolates were grown for 24 hours in TSB at 37°C and 5μl were spotted onto square petri plates (VWR International, USA) with TSA media in a 6 x 6 grid fashion and allowed to dry ...
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bioRxiv - Bioengineering 2023Quote: ... conditioned media was centrifuged at 3000 RPM for 10 minutes at 4°C and the supernatant was filtered using 0.2 μm syringe filter (VWR, 28143-310). Sample placed in ultracentrifuge tubes (Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: Tissue for HCR was cryosectioned at 30 μm at -20°C taking coronal sections that were mounted on Superfrost® slides (VWR). The mounted slides were allowed to dry for 24hrs at room temperature before being stored at -80°C before HCR ...
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bioRxiv - Plant Biology 2023Quote: ... Apogee grain were left for 2 days at 5°C in water for imbibition before being placed individually onto cotton in a 24-well tissue culture plate (VWR, USA) and left to germinate for 3 days under high humidity conditions (<90% relative humidity ...
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bioRxiv - Immunology 2024Quote: ... tubes with mAb were incubated on a rotator at 4 °C overnight on a rotator (Model 13916- 822, VWR, United States). These mAb included anti-histone H3 (1:50 ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR) for cohesin trimers and a HiLoad 16/600 Superdex 200 column (GE Healthcare ...