Labshake search
Citations for VWR :
1 - 50 of 68 citations for Human CMTR2 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cells expressing the various shRNAs were selected with 0.2 mg/ml hygromycin (cat#K547-20ml, VWR) at 72-hours post transfection and maintained at 0.8 mg/ml of hygromycin after cell line purification.
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bioRxiv - Microbiology 2023Quote: Plasmid extraction (Omega, VWR), endonuclease digestion (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant human TNF-alpha (PeproTech/VWR) was used at a concentration of 10 ng/ml.
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA was amplified and purified using EZNA endo-free plasmid maxi kit (VWR International).
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bioRxiv - Plant Biology 2019Quote: ... Colonies were evaluated by colony PCR followed by plasmid isolation from overnight culture using peqGOLD Plasmid Miniprep Kit I (VWR). All sequences were confirmed by Sanger sequencing (Sequencing Core Facility ...
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bioRxiv - Genetics 2022Quote: ... or hygromycin (FIREWach plasmids, 200 ng/μl, VWR) starting two days after transduction ...
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bioRxiv - Biochemistry 2024Quote: ... peqGOLD Plasmid Miniprep Kit I (VWR Life Science). Successful mutagenesis was verified by Sanger sequencing.
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
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bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... gentamycin (10 μg/ml for plasmid maintenance in E. coli; 100 μg/ml for plasmid maintenance in P. aeruginosa, VWR, Cat. No. 97062-974).
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bioRxiv - Developmental Biology 2021Quote: The differentiated human iPSCs were fixed on glass coverslips (VWR, Denmark) in 4% PFA for 15 min at RT and stored at 4°C until use ...
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bioRxiv - Cell Biology 2024Quote: ... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...
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bioRxiv - Microbiology 2021Quote: Pooled normal human plasma was obtained from Pacific Hemostasis (VWR #95059-698). Factor X and prothrombin-deficient plasma were obtained from Haematologic Technologies (#FX-ID and #FII-ID) ...
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bioRxiv - Immunology 2022Quote: ... Human colon tissue samples were fixed in 4%(w/v) PFA (VWR International) for 24h at RT ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human alveolar macrophages (AMs) were lysed in RIPA buffer (VWR, Radnor, PA, USA) supplemented with protease (Roche Diagnostics GmbH ...
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bioRxiv - Genetics 2020Quote: ... pelleted and purified by the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA). Inserts of the isolated plasmids were verified by sequencing ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transfected using jetPRIME® transfection reagent (VWR Scientific Cat # 89129-920) or Lipofectamine™ 2000 Transfection Reagent (Thermo Fisher Scientific #11668027 ...
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bioRxiv - Microbiology 2021Quote: ... histolytica expression plasmid pEhEx backbone (38) using the Gibson Assembly Ultra Kit (VWR). Primers used for Gibson cloning are described in Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were maintained in liquid culture with the appropriate antibiotics (VWR, Radnor, PA). List of plasmids are in Table S1.
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bioRxiv - Systems Biology 2022Quote: Yeast with plasmids were grown in a 96-well deep well plate (VWR) containing 100μl of SC-Ura medium with 2% glucose and grown for ~12 hours at 30°C in a shaking incubator set at 250 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... along with VSV-G and pUVMC packaging plasmids via Jetpei (VWR 89129-916).
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bioRxiv - Developmental Biology 2021Quote: ... DAOY human medulloblastoma cell line was obtained from ATCC and was cultured in EMEM (VWR) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... 10^5 human SHSY-5Y cells seeded on 18 mm #1 round cover glass (VWR) in a 12 well culture plate ...
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bioRxiv - Neuroscience 2020Quote: Snap frozen human DRGs were sectioned onto superfrost plus glass slides (VWR International, Leuven, Belgium) at 10μm thickness ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips (VIZGEN 2040003) ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips (VIZGEN 2040003) ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips ...
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bioRxiv - Genomics 2023Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR 25608-930) and sectioned on a Leica cryostat at -17C at 10 μm onto Vizgen MERSCOPE coverslips ...
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bioRxiv - Molecular Biology 2021Quote: ... Both Jurkat and primary human T cells were treated with 100 μg ml-1 cycloheximide (VWR) 5 minutes before harvesting ...
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bioRxiv - Cancer Biology 2021Quote: ... paraffin embedded human trephine and tonsil tissues were sliced on to Superfrost Plus® slides (VWR) and incubated at 60°C for 45min ...
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bioRxiv - Biophysics 2021Quote: Peripheral blood monocytes were isolated from human blood using 15 Ml Ficoll-Paque (VWR, Darmstadt, Germany) centrifugation gradient ...
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bioRxiv - Microbiology 2019Quote: The human monocyte cell line U937 (ATCC CRL-1593.2) was cultured in RPMI 1640 media (VWR) supplemented with 10% FBS at 37C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Human neuroblastoma SH-SY5Y cells (ATCC, CRL-2266) were cultured in DMEM medium (VWR, 0101–0500) supplemented with 10% FBS (Gemini Biosciences ...
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bioRxiv - Physiology 2022Quote: ... the isolation was performed according to the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA) and verified through sequencing.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and human keratinocyte growth supplement (HKGS) 100X (S-001–5) from Cascade Biologist were procured from VWR Corporation (Missouri ...
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bioRxiv - Genetics 2020Quote: HCT116 cells were transfected with px459sgCTNNB1 and the two donor template encoding plasmids KOZAK_FLAG_mClover3 and KOZAK_V5_mCherry using TransIT (731-0029; VWR) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: Caco-2 cells or human ileum organoids were lysed in 1 mL 20 mM Suprapur NaOH (VWR International) for 2 hours ...
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bioRxiv - Biochemistry 2019Quote: ... spectra of native, human BACE1 (6.12 μM, 30 μl; Acro Biosystems, USA) in 50 mM sodium acetate (pH 4.0; VWR, UK) was obtained using a J-1500 Jasco CD spectrometer and Spectral Manager II software ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Human foreskin fibroblasts (HFF, kindly provided by Denise Galloway) were maintained in Minimum Essential Media-α (MEM-α; VWR) supplemented with 20% FBS (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... we transfect the cells with either WT or mutant SOD1 pCI-Neo plasmid using JetOptimus transfection reagent (VWR) according to the manufacturer’s instructions (31) ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A vial of human primary nasal epithelial cells was thawed and aliquoted into multiple T25 cell culture flasks (VWR, UK). Cells were maintained in AECGM until confluent ...
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bioRxiv - Cell Biology 2021Quote: 3,000 ESCs/well mixed with 0.3 μg Wnt3a-beads/well were seeded onto human fibronectin (10 μg/mL, cat. um. 7340101, VWR/SLS) coated 8-well slides (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Human neuronal cells SH-SY5Y (ATCC, Manassas, VA, #CRL-2266) were cultured in DMEM medium (VWR, Radnor, PA, #0101-0500) supplemented with 10% FBS (Gemini Biosciences West Sacramento ...