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Citations for VWR :
1 - 50 of 175 citations for Hepatitis E Virus Capsid Protein ORF2 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Genetics 2021Quote: ... anti V5-tag (VWR, MEDMMM-) and Anti-pgk1(Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10 % FCS (VWR International, S181B) and 1 % Pen/Strep (Life Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... H&E routine stain was performed using Leica ST5010 Autostainer XL and Leica ST Infinity H&E Staining System kit (VWR#3801698) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Neuroscience 2021Quote: ... A virus or tracer was injected using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Cell Biology 2022Quote: ... and digested with 1 mg/mL pronase E (39052, VWR) per 1 g tissue for 6-8 h at 37 °C and 5% CO2 under constant agitation ...
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bioRxiv - Biochemistry 2022Quote: ... Virus-containing supernatants were filtered (0.45 μm pore size cellulose-acetate filter (VWR)) ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with HEPES and 10% fetal calf serum (FCS) (VWR) 37°C/5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... rabies virus was delivered by pressure injection (Nanoject II Variable Volume Automatic Injector, VWR) in 23 nl increments over a 3 min and 10 sec interval to a final volume of 500 nl ...
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bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... with 10% fetal calf serum (FCS premium, S. Amer. Orig, VWR), 2 mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... The virus band was extracted and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... the virus-particle-containing medium was collected and filtered using a 0.45 µm syringe filter (VWR), and then centrifuged at 25,000 × g for 90 min at 4°C ...
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bioRxiv - Genomics 2024Quote: ... 5 mM Na3 citrate 2H2O) with ∼75u/mL Pronase E (VWR, Radnor, PA) was added to each sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Tygon tubing was replaced by flexible pumping tubing (Masterflex Tygon E-3603, VWR). Outflow tubing were connected to outlet ports ...
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bioRxiv - Synthetic Biology 2019Quote: ... the supernatant (containing secreted AAV virus) was collected and filtered through a 0.45-μm syringe filter (VWR). AAV virus was aliquoted into sterile Eppendorf tubes (0.5 mL/tube) ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Microbiology 2023Quote: ... Vero E6 (ATCC CRL- 1586) was cultured in DMEM supplemented with 5 % FCS (VWR) and 1 % penicillin−streptomycin−glutamine (PSG ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Biophysics 2019Quote: ... The vial with the cell suspension was connected via Tygon S3 E-3603 tubing (VWR, inner diameter 1/32" ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Cell Biology 2020Quote: ... virus-containing supernatant was collected 48 h later and filtered through a 0.45 μm polyethersulfone filter (VWR 28145-505). For protein-coding constructs ...
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bioRxiv - Microbiology 2023Quote: ... The virus containing band was extracted from the gradient and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Genomics 2021Quote: ... and 5mL fractions were removed and filtered (70uM) into RPMI supplemented with 2% FCS (VWR) three times ...
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bioRxiv - Microbiology 2023Quote: ... then treated with Pronase E (100µg/ml in Tris-HCl (pH 7.2) + 0.06% NaCl) (VWR Chemicals) for 2 hours at 60C to remove proteins covalently bound to PG ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (which contains the majority of virus) was filtered through a 0.2 μM PES filter (VWR, cat. #97066-214). 25 mL of 40% PEG8000 (JT Baker ...
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bioRxiv - Synthetic Biology 2023Quote: ... then plated onto YPM supplemented with 60 µg mL-1 5-fluorocytosine (5-FC) (TCF0321, VWR). Colonies that emerged were then transferred onto a new YPM 5-FC agar plate for clonal isolation ...
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Immunology 2019Quote: ... Cell monolayers were processed for ICC staining at 24 to 72 hpi depending on virus replication kinetics (as previously described [94]) or stained with Giemsa stain (VWR; 35086). Relative plaque titre was calculated as the plaque titre of a virus stock under the indicated condition divided by its titre under a control condition ...
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bioRxiv - Genetics 2019Quote: ... Eluates were analysed by SDS-PAGE followed by western blotting with antibodies anti-V5 tag (VWR, MEDMMM-0168-P), anti-Pgk1 (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... and were treated for 2hrs at 60°C with activated Pronase E (100µg/ml final concentration, pre-treated for 2hrs at 60°C in the digestion buffer; supplier: VWR). The sacculi were incubated at 100°C for 10min to inactivate the Pronase E and were washed 3 times with 1ml of MQ water ...
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bioRxiv - Biochemistry 2022Quote: ... and were treated for 2hrs at 60°C with Pronase E (100µg/ml final concentration, pre-treated for 2hrs at 60C in the digestion buffer; supplier: VWR). The sacculi were incubated at 100°C for 10min to inactivate the Pronase E ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Biophysics 2021Quote: ... The sandwich consists of a bottom layer of PAA gel (E ≈ 1800 Pa) and a top layer of a 12 mm round glass cover slip (VWR).
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic Hydra from strains Tg(actin1:GFP)rs3-in and Tg(tba1c:mNeonGreen)cj1-gt were dissociated using Pronase E (VWR, E629-1G) as previously described (Siebert et al. ...
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bioRxiv - Physiology 2023Quote: ... 8 μl of the final polyacrylamide mix were pipetted on 35 mm glass bottom dishes (Cell E&G LLC VWR, USA) which were pretreated with 0.1 M NaOH ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The tube was topped off with 45:45:10 1xMMR:L15:FCS to inactivate the papain and strained threw a 70um sieve (VWR 10199-656). The sieve was washed with 25ml 45:45:10 1xMMR:L15:FCS ...
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bioRxiv - Microbiology 2023Quote: ... A surface capture lawn was prepared with 50 ug/mL of goat anti-Human IgG Fc secondary antibody (VWR, 103255-066) in 10 mM sodium acetate (pH 4.5)/0.01% Tween ...
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bioRxiv - Physiology 2020Quote: ... 5-μm sections were stained with hematoxylin and eosin (H&E) using the Leica Autostainer and mounted in DePeX (VWR, Lutterworth, UK). Adipocyte diameter and pancreatic β-cell islet number and size were quantified using ImageJ software as previously described (7 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal (VWR GenScript A01622-40); western blot ...
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bioRxiv - Molecular Biology 2022Quote: ... and anti-mouse (1:10,000; VWR)-conjugated to horseradish peroxidase HRP.
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bioRxiv - Microbiology 2021Quote: ... supplemented with 100 μg/mL of 5-florocystosine (5-FC) and grown at 24°C as listed in Supplemental Table S1 (Stork et al. 2008) (VWR, Radnor, PA, USA). A 10 mg/mL stock solution of 5-FC was prepared in distilled water and filtered sterilized ...
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bioRxiv - Immunology 2022Quote: ... gentamycin (10 μg/ml for plasmid maintenance in E. coli; 100 μg/ml for plasmid maintenance in P. aeruginosa, VWR, Cat. No. 97062-974).
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bioRxiv - Biophysics 2023Quote: ... The concentration of total proteins was determined with the BCA Protein Assay Kit (VWR International GmbH, Darmstadt, Germany). According to the total protein amount in each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).