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Citations for VWR :
651 - 700 of 942 citations for HDM Fluorescent Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... 50 µL of the agarose solution was then transferred over to each well of the 96 well U bottom plate (10062-900, VWR, PA, USA) then let solidify at room temperature (RT) ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cultures expressing CcaS-CcaR system variants were cultured in darkness for 12-14 h in LB (1% Tryptone, 0.5% Yeast Extract, 1% Sodium Chloride) with appropriate antibiotics in 96 well plates (VWR, Cat#732-3802), with one 3 mm glass bead and 750 μl media per well at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes at room temperature with gentle agitation on a plate rocker and mounted on microscope slides (VWR REF# 48311-703) with mounting medium containing DAPI ...
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bioRxiv - Microbiology 2019Quote: ... One single colony was picked and incubated for 8 to 10 h at 30°C in either 4.5 ml or 1 ml BHI containing 154mM acetate in reaction tubes or Deepwell-plates (VWR International, Pennsylvania, USA), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 2000 cells per well in a volume of 100 µl were seeded on flat-bottomed 96-well plates (VWR, Radnor, USA) and incubated in a 95% humidified atmosphere with 5% CO2 at 37 °C for 24 h ...
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bioRxiv - Plant Biology 2023Quote: ... from accessions grown in UC Davis or UC Riverside greenhouses and floated overnight in 1 mL deionized water in a 24-well tissue culture plate (VWR #10062-896) with a plastic lid to prevent evaporation of the water ...
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bioRxiv - Neuroscience 2023Quote: ... and BHK-B19L cells (see above) were plated in 10 cm plates coated in poly-L-lysine in normal medium (10% fetal bovine serum (VWR 16777-014) and antibiotic-antimycotic (Thermo 15240096 ...
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bioRxiv - Genetics 2023Quote: ... A 750 μL suspension of each sample was then transferred to a 96-well filter plate (Norgen, #40008) where the media was eliminated by applying vacuum (VWR, #16003-836). Immediately after the media was eliminated ...
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bioRxiv - Genomics 2023Quote: ... cells were harvested by filtration in 96-well filter plate (Norgen, #40008) where the media was eliminated by applying vacuum (VWR, #16003-836). The filter plates were immediately flash frozen in liquid nitrogen and stored at -80°C.
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bioRxiv - Genetics 2023Quote: ... Small pieces of cotton wool soaked with sterile water were placed in each well of a 24-well tissue culture plate (VWR International, US). One wheat seed was placed with the crease facing downwards into each well ...
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bioRxiv - Microbiology 2023Quote: ... were transfected into single wells (3.5 cm) of the Corning Costar 6-well cell culture plate containing HEK293T/17 cells using the JetPrime Transfection reagent (VWR, Randor, PA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 400,000 cells were seeded on ethanol-sterilised coverslip in 6 well 3.5 cm diameter plate containing 22 × 22 mm glass coverslips (VWR. Cat# 631-0125). 2 μg of DNA and 6 μg of PEI were diluted in 0.25 ml of Opti-MEM™ Reduced serum medium (Gibco™) ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were seeded at 1×106 cells/well in 24-well plates and maintained in X-VIVO-15™ media (CA12001-988, VWR, USA). Four treatment groups were used ...
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bioRxiv - Microbiology 2023Quote: ... The last column of the plate was not coated since it contained the medium alone to allow the addition of sodium sulfite (VWR 0628-500g) during the run ...
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bioRxiv - Neuroscience 2023Quote: ... wells of 48-well mwMEA plates (Cytoview Cat#MEA 48, M768-tMEA_48B, Axion Biosystems) and 18mm Menzel glass coverslips (#6311342, VWR International, Radnor, USA) were coated with poly-L-lysine (0.5 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of M9 was added to the plate and the bacterial lawns were scraped with a sterile L spreader (VWR 490007-358). Bacterial slurry was pipetted and centrifuged at 13000 x g for 4 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, catalog # 97062-376, VWR) (5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... then tumors were measured every 2-3 days using a digital caliper (VWR, Radnor, PA, USA). Tumor volume was calculated with the formula 0.5 × L × W2 where L is the longest diameter and W is the diameter perpendicular to L ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue was homogenized in 2 ml lysis buffer using a Dounce tissue grinder (7ml, VWR). This suspension was supplied with a 1.8M sucrose solution (4 mL) ...
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bioRxiv - Cancer Biology 2020Quote: ... Beads were washed 4X in Kischkel buffer + 2 μg/ml Aprotinin (VWR, Radnor, Pennsylvania, 97062-752) + 1 μg/ml Pepstatin (VWR 97063-246 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Caco-2 cells were cultured in EMEM (ATCC, 30-2003) with 20% FBS (VWR 89510-186), 1% Pen/Strep (Life Technologies 15140122) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Supernatant was supplemented with 2 mM calcium chloride and filtered by a glass microfiber filter (VWR). Samples were then loaded by gravity flow onto 5 ml anti-protein C antibody affinity resin ...
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bioRxiv - Cell Biology 2019Quote: ... while covered with 1.5 ml of imaging media and 2 ml of white mineral oil (VWR). An objective heater (Bioptech ...
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bioRxiv - Immunology 2020Quote: ... were separately stored in 2 mL pre-filled 2.4mm metal beads tubes (VWR International, Radnor, PA), and quickly snap-frozen in liquid nitrogen (40) ...
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bioRxiv - Cell Biology 2022Quote: ... glucose was replaced with 2% (w/v) galactose (ITW reagents) or 1.5% (w/v) raffinose (VWR), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... mice expressing hM4D(Gi) were intraperitoneally injected with 2 mg/kg CNO (89160; VWR International, LLC) and returned to their home cages ...
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bioRxiv - Cell Biology 2023Quote: ... cells grown in 2- or 4-well chamber slides (model 1 German borosilicate; Lab-Tek; VWR) were fixed with freshly prepared 4% formaldehyde in PBS solution (using 16% Methanol-free Formaldehyde ...
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bioRxiv - Neuroscience 2024Quote: ... pre-chilled 2 ml Pre-Filled Bead Tubes with 2.8 mm Ceramic Beads (VWR, Radnor, PA), and weighed ...
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bioRxiv - Cancer Biology 2020Quote: ... DAB (Bright-DAB Substract Kit, IL ImmunoLogic, VWR) was used for protein detection ...
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bioRxiv - Microbiology 2023Quote: ... subtilis using the peqGOLD Bacterial DNA Kit (VWR). Plasmid DNA was isolated using the Nucleospin Extract Kit (Macherey-Nagel) ...
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bioRxiv - Biochemistry 2024Quote: ... peqGOLD Plasmid Miniprep Kit I (VWR Life Science). Successful mutagenesis was verified by Sanger sequencing.
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bioRxiv - Cell Biology 2020Quote: ... Solutions were mixed by shaking the compound plate for 1 min at room temperature at 1200 rpm using a microplate shaker (cat. 12620-926, VWR, West Chester, PA). A typical plate layout map used for screening experiments is shown in Figure 1 ...
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bioRxiv - Biophysics 2019Quote: ... cells were grown in 6-well plates (STARLAB; Milton Keynes, UK) or 30 mm culture dishes (STARLAB) on untreated 30 mm borosilicate coverslips (Thickness # 1, VWR International; Radnor, PA) or on poly-D-lysine coated FluoroDishes (FD35-PDL-100 ...
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bioRxiv - Bioengineering 2021Quote: ... Microtiter plates were filled with 160 μl of bloodmeal per well and sealed with a membrane (Parafilm M, PM999, VWR, Amsterdam, the Netherlands) that was stretched to about 250% its original dimensions in both directions using a custom build device (Figure S1A ...
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bioRxiv - Cell Biology 2019Quote: ... Single cells were sorted into 200 μL fresh culture medium in one well of a 96-well flat bottom plate (VWR Catalog #29442-054).
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bioRxiv - Cancer Biology 2021Quote: 1×105 A549 or H460 cells in 200 µl of serum-free growth medium were seeded in the insert of a transwell plate with 8 μm pores (VWR International, Radnor, PA). These cells were coincubated with AvFc or CTX at 30 nM for 2 hours at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... from control and treatment cultures were harvested at both 24- and 48-hour time points and mixed with 400μL of culture media in a 96-deep well storage plate (VWR International, Radnor, PA, USA) and stored frozen at −20°C for cytokine assays by ELISA ...
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bioRxiv - Microbiology 2021Quote: ... Vero cells were seeded at 60% confluence in 24-well plates equipped with 13 mm round glass coverslips (VWR, cat n. 631-0150). Cells were washed once with PBS before incubation with BetaCoV/Australia/VIC01/2020 diluted in PBS at an MOI=5 ...
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bioRxiv - Immunology 2020Quote: ... Fused cells were then seeded on 96 well flat bottom plates onto mouse peritoneal cells as feeder layer in hypoxanthine-aminopterine-thymidine (VWR, Radnor, Pennsylvania, USA) medium for the selection process.
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bioRxiv - Genetics 2019Quote: We performed cell culture using the conditions described above in 12-well plates containing sterile 18-mm glass cover slips (VWR, Cat#48380-046). We fixed cells for 10 minutes in 4% paraformaldehyde (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: Antibiotic tolerance assays using co-cultures were performed using a membrane-separated 12-well tissue plate cultures containing 0.1 μm pore PET membranes (VWR, Cat. No. 10769–226), following previously protocols (Meirelles et al. ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of the enriched seawater and 100 μL of the diatom-concentrated seawater were then inoculated on agar plates (1% agar – VWR Agar for bacteriology) supplemented with Marine Broth 2216 (VWR ...
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bioRxiv - Genomics 2022Quote: ... HeLa S3 or HeLa S3 + TOP2A- Venus cell linescells were either grown or spun onto 22×22mm No 1.5 coverslips in 6-well plates (VWR Cat No 48366-277). (cell cycle analysis experiments ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (4×105 cells/well) were laid in sterile round bottom 96-well tissue culture treated plates (VWR, The Netherlands, cat #734-2184) in Dutch modified RPMI 1640 medium containing 50 µg/mL Gentamicine ...
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bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
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bioRxiv - Microbiology 2024Quote: The bacterial strains were revived by streaking a loopful of frozen glycerol stock on Brain Heart Infusion (BHI) agar plates (15 g/L Agar, 37 g/L BHI Broth, VWR Life Science, USA) and incubated at 37±2 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... 200 µL aliquots were then transferred to the wells of a NUNC flat-bottomed 96-well microtiter plate (VWR International GMBH, Darmstadt, Germany). Subsequently ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... A single colony of bacteria from each plate was cultured under anaerobic conditions (Anaerobic Hungate Culture Tubes with air displaced by CO2, VWR, Radnor, PA, USA), shaking at 35°C for 3 nights and was then retained at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Amplified emulsion mixture was broken and purified by adding 1 mL of 2-butanol (VWR, AA43315-AK), 50 μL of AMPure XP SPRI (Beckman Coulter ...
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bioRxiv - Genetics 2019Quote: ... All digested products were electrophoresed on 2% agarose gels using Agarose SFR™ (VWR Life Science, USA) for 2 hours at 80V ...