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Citations for VWR :
51 - 100 of 138 citations for Frizzled 8 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 100ng/mL recombinant human Noggin (VWR International; 120-10C), 100ng/mL recombinant human FGF10 (Peprotech ...
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bioRxiv - Cancer Biology 2021Quote: ... 8 µm tissue sections were placed on positively charged slides (VWR International, Radnor, PA) and air dried ...
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bioRxiv - Biochemistry 2022Quote: ... The stirrer was an 8 mm x 3 mm pivot bar (VWR 37119-6183), stirring at 650 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... with film (GeneMate Blue Lite Autorad Film, F-#9024-8×10, VWR, Radnor, PA). Independent neuronal cultures or HEK293T transfections from separate passages were considered biological replicates ...
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bioRxiv - Immunology 2022Quote: ... isolated neutrophils were seeded into 8 well lab-Tek II chamber slide (VWR international) coated with 2% poly-lysine (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: HeLa cells were seeded in 8-well Lab-Tek chamber slides (VWR, Radnor, PA) at a density of 30,000 cells per well one day prior to experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C with CO2 (8% v/v) on an orbital shaker (VWR Scientific). One day before transfection ...
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bioRxiv - Physiology 2024Quote: Twenty-four-well plates and 8-μm pore-size polyvinylpyrrolidone free polycarbonate membranes (VWR) were used ...
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bioRxiv - Cell Biology 2021Quote: Human MSCs were fixed with 4% paraformaldehyde (PFA, VWR International) in deionized (DI ...
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bioRxiv - Biochemistry 2022Quote: ... The human cell lines HEK293T were maintained in DMEM (VWR) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... samples were diluted at 3-fold in 8 serial dilutions using DMEM (VWR, #45000-304) in duplicates with an initial dilution of 1:10 in a total volume of 60 μl ...
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bioRxiv - Cell Biology 2021Quote: ... a 24-well insert with an 8-μm-pore membrane Trans-wells (VWR International Ltd) was coated with 50 μl of CellMatrix/PureCol collagen mixture (1:1 ...
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bioRxiv - Immunology 2022Quote: ... samples were diluted at 3-fold in 8 serial dilutions using DMEM (VWR, #45000-304) in duplicates with an initial dilution of 1:10 in a total volume of 60 l ...
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bioRxiv - Immunology 2022Quote: ... samples were diluted at 3-fold in 8 serial dilutions using DMEM (VWR, #45000-304) in duplicates with an initial dilution of 1:10 in a total volume of 60μl ...
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bioRxiv - Immunology 2021Quote: ... samples were diluted at 3-fold in 8 serial dilutions using DMEM (VWR, #45000-304) in duplicates with an initial dilution of 1:10 in a total volume of 60 μl ...
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bioRxiv - Immunology 2021Quote: ... samples were diluted at 3-fold in 8 serial dilutions using DMEM (VWR, #45000-304) in duplicates with an initial dilution of 1:10 in a total volume of 60μl ...
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bioRxiv - Immunology 2021Quote: ... samples were diluted at 3-fold in 8 serial dilutions using DMEM (VWR, #45000-304) in duplicates with an initial dilution of 1:10 in a total volume of 60 μl ...
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bioRxiv - Biochemistry 2024Quote: ... Fiberlite F21-8×50y rotor) and applied to a 5 ml HisTrap Column (VWR, Cytiva) mounted on an Aekta Purifier 100 FPLC system.
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bioRxiv - Developmental Biology 2021Quote: The differentiated human iPSCs were fixed on glass coverslips (VWR, Denmark) in 4% PFA for 15 min at RT and stored at 4°C until use ...
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bioRxiv - Cell Biology 2024Quote: ... P/S and 2% human serum (VWR/BioWest, cat. #S4190-100). The culture insert’s apical site was supplemented with 300uL and the basolateral site with 800uL media ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were resolved by 8% SDS-PAGE and transferred to nitrocellulose membranes (pore 0.2μm, VWR #27376-991). Membranes were blocked in TBS-Tween (25mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 x 107 cells per replicate were resuspended in lysis buffer (8 M urea (VWR, 0568-500G), 50 mM Tris-HCl pH8.0 ...
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bioRxiv - Microbiology 2021Quote: Pooled normal human plasma was obtained from Pacific Hemostasis (VWR #95059-698). Factor X and prothrombin-deficient plasma were obtained from Haematologic Technologies (#FX-ID and #FII-ID) ...
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bioRxiv - Physiology 2021Quote: ... Cryostat-cut sections (8-10μm) were mounted directly onto poly-L-lysine-covered glass slides (VWR, Leuven, Belgium) by the Institute of Metabolic Science Histology Core ...
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bioRxiv - Neuroscience 2022Quote: ... slices were homogenized in 1.5 mL 8 M Urea buffer using a Wheaton Potter-Elvehjem Tissue Grinder (VWR). The lysates were sonicated and centrifuged at 13000 rpm for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... infected HCT-8 cells in 96-well plates were fixed with sterile 4% paraformaldehyde (PFA; VWR Chemicals #28794.295) in PBS for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Human colon tissue samples were fixed in 4%(w/v) PFA (VWR International) for 24h at RT ...
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bioRxiv - Biophysics 2020Quote: ... equal amounts of the fibrillated sample were aliquoted into 8 tubes and the fibrils were pelleted by centrifugation (13,500 rpm, 15 min) in a MicroStar12 table centrifuge (VWR) and the supernatants were removed and the concentration of un-fibrillated monomer was measured to determine the exact amount of fibrillated protein ...
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bioRxiv - Microbiology 2020Quote: ... and 8 h by serially diluting cultures in 10-fold steps in 200 μl sterile PBS (VWR International, USA), before enumeration of c.f.u ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR was performed on previously generated cDNA (8 ng) using KOD Hot Start DNA Polymerase (VWR CA80511-386). The primer sequences for the target transcripts and number of cycles are outlined in Table 3 ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Primary human alveolar macrophages (AMs) were lysed in RIPA buffer (VWR, Radnor, PA, USA) supplemented with protease (Roche Diagnostics GmbH ...
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bioRxiv - Cell Biology 2019Quote: ... 50 µL of the collagen-I mixture was pipetted into the bottom of the wells of an 8-chamber slides (VWR) to coat the surface and allowed to gel for 20 minutes at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... animals were randomly assigned to individual cages under one of four groups (n=8 per group): Wild-type (WT) C57BL/6 mice with access to a free running wheel (VWR) or a locked wheel (SED) ...
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bioRxiv - Physiology 2020Quote: ... Sections were then post-fixed in 4% PFA/PBS before washing with PBS/Tween 0.1% and then acetylated (1.325% triethanolamine [pH 7-8]; 0.25% acetic anhydride; 0.175% HCl in autoclaved water; VWR Chemicals, Lutterworth, UK) for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
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bioRxiv - Neuroscience 2024Quote: ... the enriched PBMCs at the middle layer were carefully removed and washed twice in 1% BSA (VWR, 9048- 45-8) in DMEM (Corning ...
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bioRxiv - Physiology 2024Quote: ... were then exposed to cold treatments of -15°C for up to 48 hours (in increments of 4, 8, 12, or 48 hours) in a cooling circulator (VWR Scientific 1167P Heating/Cooling Recirculating Water Bath ...
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bioRxiv - Microbiology 2024Quote: ... was diluted 1:10 and 200 µL aliquots of the diluted sample were pipetted into 8 wells of a flat bottom 96-well plate (VWR Tissue Culture Plate ...
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bioRxiv - Molecular Biology 2024Quote: Boiled protein samples were analyzed by SDS PAGE on 6% acrylamide stacking gel and 8% acrylamide resolving gel and transferred to 0.45μm nitrocellulose blotting membranes (VWR CA10061-120). Membranes were incubated with primary antibody overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... DAOY human medulloblastoma cell line was obtained from ATCC and was cultured in EMEM (VWR) supplemented with 10% FBS (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... 10^5 human SHSY-5Y cells seeded on 18 mm #1 round cover glass (VWR) in a 12 well culture plate ...
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bioRxiv - Neuroscience 2020Quote: Snap frozen human DRGs were sectioned onto superfrost plus glass slides (VWR International, Leuven, Belgium) at 10μm thickness ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips (VIZGEN 2040003) ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips (VIZGEN 2040003) ...
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bioRxiv - Neuroscience 2022Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR,25608-930) and sectioned on a Leica cryostat at −17 C at 10 um onto Vizgen MERSCOPE coverslips ...
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bioRxiv - Genomics 2023Quote: Human postmortem frozen brain tissue was embedded in Optimum Cutting Temperature medium (VWR 25608-930) and sectioned on a Leica cryostat at -17C at 10 μm onto Vizgen MERSCOPE coverslips ...
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bioRxiv - Cell Biology 2021Quote: ... 40% glycerol and 412mM β-mercaptoethanol) before being resolved by 8% SDS-PAGE and transferred to nitrocellulose membranes (pore 0.2μm, VWR #27376-991). Membranes were blocked in TBS-Tween (25mM Tris-HCl pH 8 ...