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Citations for VWR :
551 - 600 of 1242 citations for Endothelin 1 ET 1 ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and sequentially washed with RIPA (PBS pH 7.4 with 0.5% sodium deoxycholate [Thermo], 0.1% sodium dodecylsulfate [Fisher Scientific, Waltham, MA], 1% Nonidet P-40 substitute [VWR]), high salt PBS (PBS pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were rocked every 15 minutes for 1 hour before a semi-solid overly containing 0.5% Methylcellulose in viral growth media (VWR) was applied ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cultures were fixed by adding 2 μL culture into 198 μL 1 × phosphate-buffered saline (K813-500ML, VWR) in a 96-well U-bottom plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... we added 40 mg of powder yeast extract g−1 dry soil as a C and nutrient source (VWR). We chose yeast extract over glucose to provide more varied forms of C and thus stimulate more diverse microorganisms (Fierer et al. ...
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bioRxiv - Pathology 2022Quote: ... Color development was obtained by incubation with KPL Sure Blue TMB Microwell Peroxidase Substrate 1-Component (95059-286, VWR), and the reaction was stopped using 2M H2SO4 (80012010.2500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were serum-starved 16 hours prior to plating with 1% bovine serum albumin (VWR, Cat. no. 0332-100G) added for protein supplementation ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150] ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were run on 7.5-12% polyacrylamide gels for ∼1 hour at 150V and transferred to PVDF membranes (VWR) for 1 hour at 330 mA on ice ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... washed twice in distilled water for 2 minutes and dehydrated through 70% and 95% ethanol (4.5 and 1 minute respectively) before being incubated in Histoclear (VWR) for 3 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... centrifuged for 5 min at 4000 x g-1 and filtered over a 0.2 µm cellulose acetate filter (VWR). 1 ml aliquots were concentrated in a speed-vac (Eppendorf ...
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bioRxiv - Bioengineering 2022Quote: ... Optical density (OD) was measured in technical triplicates in 1-mL polystyrene cuvettes in a V-1200 spectrophotometer (VWR) at 600 nm ...
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bioRxiv - Immunology 2022Quote: ... Cell density was adjusted to 1×106 cells/ml and added to the FACS tubes (Falcon® Brand, VWR). After pelleting ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... washed twice in distilled water for 2 minutes and dehydrated through 70% and 95% ethanol (4.5 and 1 minute respectively) before being incubated in Histoclear (VWR) for 3 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 1 mL of cold brain heart infusion (BHI) broth (VWR, cat. no. 90003-040) per infected T175 cm2 flask ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a 1:5 volumetric ratio (chloroform:Trizol) for aqueous separation of RNA via centrifugation in heavy gel phase-lock tubes (VWR). Aqueous phase was transferred into isopropanol followed by RNA purification using a Qiagen RNeasy Mini Kit as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then rinsed 3x in TBS and incubated for 1 hour in anti-mouse IR680 (VWR 102971-020) and anti-rabbit IR800 (VWR 103011-494 ...
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bioRxiv - Neuroscience 2023Quote: ... 1:2 dilution) was injected into the three DCN using a Micro4 controller and UltraMicroPump 3 (VWR, Radnor, PA). Glass needles were made from 1-mm outer diameter glass pipettes (Wiretrol II ...
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bioRxiv - Microbiology 2023Quote: ... cells were scraped into 1 mL fresh KA medium using disposable cell scrapers with 20 mm blade width (VWR). Cells were pelleted with the respective supernatants discarded and then flash frozen in a dry ice-ethanol bath to preserve RNA integrity and stored at −80°C ...
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bioRxiv - Physiology 2023Quote: Post-ultracentrifugation supernatant (S2) from brain lysates were thawed on ice alongside 1 mM Thioflavin T (VWR 103802-652). Thioflavin T (ThT ...
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bioRxiv - Microbiology 2023Quote: ... XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] solution (0.5 mg XTT/mL 1× PBS with 25 µM menadione) (XTT sodium salt, VWR) was added at 150 µl per well and incubated at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins production was induced with 1 mM [F1] IPTG 3h [F2] treatment (Isopropil-β-D-tiogalattopiranoside, VWR, Pennsylvania, USA). Then ...
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bioRxiv - Cell Biology 2022Quote: ... THP-1 monocytes were differentiated into macrophages by treatment with 50 nM phorbol 12-myristate 13-acetate (PMA, VWR) for 3 days at a density of 5 × 104 cells per well in a 96-well tissue culture plate (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... 1:10,000 NaOH 10N, 0.02% sodium azide) for 1 hour for each step (20%, 40%, 60%, 80%) on a nutator (VWR). Samples were then washed with 100% MeOH 2x for 1hr each and then incubated in a 2:1 dicholoromethane (DCM):MeOH solution overnight ...
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bioRxiv - Immunology 2023Quote: ... Cell pellets were diluted in 1 mL PBS and they or BALF were shaken in a glass vial (VWR) with 2 mL chloroform and 1 mL methanol containing internal standards (13C16-palmitic acid ...
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bioRxiv - Microbiology 2023Quote: ... afra leaf material was ground and extracted in dichloromethane (DCM) (1 g:20 mL, VWR International, Radnor, PA, USA) under sonication for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... washed twice in distilled water for 2 minutes and dehydrated through 70% and 95% ethanol (4.5 and 1 minute respectively) before being incubated in Histoclear (VWR) for 3 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatant containing soluble gp120 glycoprotein was then clarified by centrifugation at 7000 x g for 1 hour and filtered using a 0.45 μm vacuum filtering system (VWR). Supernatant was dialyzed against Ni-NTA buffer (50 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 mL of seed culture was used to inoculate 1 L media in a Fernbach flask (VWR 29171-854). Cells were incubated at 37 °C shaking at 210 rev/min ...
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bioRxiv - Neuroscience 2024Quote: ... samples were homogenized with a tissue grinder dounce (WHEATON® Dounce Tissue Grinder 1 mL, VWR, Radnor, Pennsylvania, USA) in 150 mM NaCl (S3014 Sigma-Aldrich - Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... in 96 well plates (VWR) containing 2 μL of PBS including 0.2% Triton X-100 and 4U of RNase inhibitor (Takara ...
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bioRxiv - Immunology 2021Quote: Untreated 384-well plates (VWR) were coated with recombinant HA proteins diluted to 2 μg/mL in PBS at 4 °C overnight ...
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bioRxiv - Microbiology 2019Quote: ... twenty-four well plates (VWR) were treated with 0.5 ml of concentrated sulfuric acid for 10 min at 60°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 96-deep-well plates (VWR) were incubated at 37°C and 700 rpm for 8 h ...
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bioRxiv - Biochemistry 2020Quote: ... Maxisorp plates (VWR, Lutterworth, UK) were coated with 5 μg/mL anti-DPEEAE neoepitope antibody (Cat n ...
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bioRxiv - Biochemistry 2023Quote: ... The plate was sealed (VWR), and growth was monitored in a plate reader (Biotek ...
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bioRxiv - Microbiology 2019Quote: ... Diluted cells were plated onto fresh LB plates (15 ml medium per plate, the diameter of the plate is 10 cm and the height 15 mm, VWR, US, Catalog number 25384-342), and multiple dilutions were used ...
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bioRxiv - Biophysics 2021Quote: ... the lipid mixture was resuspended in 1 ml of PBS and sonicated for 10 minutes (ultrasound bath USC500TH, VWR, England) to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Developmental Biology 2022Quote: ... Ganglioid frozen sections and paraffin-embedded human normal colon sections were prepared as above and then washed three times in PBS and blocked for 1-2 hours in serum (10% donkey or 10% goat) with 0.5% (v/v) Triton X-100 (VWR 0694). Slides were then incubated with primary antibody diluted in serum (10% donkey or 10% goat ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of the labeled cell suspension was gently pipetted onto 22 x 22 mm No.1 cover glass (VWR) and sealed onto glass slides with clear acrylic nail polish for confocal imaging or onto Lab-Tek chambered #1 cover glass (Thermo Fischer Scientific)(for widefield fluorescence) ...
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bioRxiv - Neuroscience 2019Quote: ... was made with a dental drill (Osada) for injection into the target (see Table 1) using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Biochemistry 2020Quote: ... All PKC assays (except the PKC-mu and the PKC-nu assay) additionally contained 1 mM CaCl2 (VWR; Cat. # 1.02382), 4 mM EDTA (Roth ...
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bioRxiv - Microbiology 2019Quote: ... Pellets were thawed on ice and resuspended in 250 μL of PBS supplemented with 1 M NaCl and 6 M Urea (VWR). The resuspended bacteria were then added to screw cap microcentrifuge lysing tubes (Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: HDL samples prepared by adding 15 μL 3 mg/mL spin-labeled apoA-I probe to 45 μL 1 mg/mL HDL and drawn into an EPR-compatible borosilicate capillary tube (VWR) (32) ...
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bioRxiv - Bioengineering 2020Quote: ... SiO2 particles were washed with methanol and centrifuged for 1 min to remove supernatant and then again mixed with methanol and vortexed (Vortex mixer, VWR) prior usage ...
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bioRxiv - Microbiology 2021Quote: Gametocytes were produced by treatment of the mice at 1% parasitemia with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) in their drinking water for two days before exsanguination ...
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bioRxiv - Physiology 2021Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 [Bio-Rad], 0.01% Molecular Grade BSA [New England Biolabs], 146 mM NaCl [ThermoFisher Scientific], 1 mM CaCl2 [VWR International] ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in ~100 μL of culture supernatant and 3 μL deposited on a pre-set 65 μL drop of SC+ 2% glucose+ 2% agar gel medium on a circular glass coverslip (#1, VWR) that was encircled by an adhesive silicon ring ...
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bioRxiv - Neuroscience 2020Quote: ... and then cryoprotected for 3 days at 4 °C in 30% sucrose solution (w/v in 1× PBS; VWR Chemicals). Brains were then embedded into optimal cutting temperature (OCT ...
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bioRxiv - Microbiology 2021Quote: ... Mice were culled 1 day later and their spleens were collected in optimal cutting temperature (OCT) compound (VWR Chemicals, UK) and stored at -80° C to test for the presence of FDC-associated IC by confocal microscopy.