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Citations for VWR :
1 - 50 of 237 citations for Dengue Virus serotype 3 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Neuroscience 2021Quote: ... A virus or tracer was injected using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Biochemistry 2022Quote: ... Virus-containing supernatants were filtered (0.45 μm pore size cellulose-acetate filter (VWR)) ...
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bioRxiv - Neuroscience 2020Quote: ... rabies virus was delivered by pressure injection (Nanoject II Variable Volume Automatic Injector, VWR) in 23 nl increments over a 3 min and 10 sec interval to a final volume of 500 nl ...
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bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... 3% SDS (151-21-3, VWR Life Science) and 100 mM Tris–HCl [pH 6.8](1.00317 ...
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bioRxiv - Microbiology 2023Quote: ... The virus band was extracted and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Genetics 2020Quote: ... Cell lysate concentrations were measured using Bradford reagent (VWR) or BCA assay (ThermoFisher) ...
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bioRxiv - Bioengineering 2023Quote: ... the virus-particle-containing medium was collected and filtered using a 0.45 µm syringe filter (VWR), and then centrifuged at 25,000 × g for 90 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: Protein lysate was collected with RIPA buffer (VWR Life Science) supplemented with 2X protease inhibitors (Pierce ...
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bioRxiv - Neuroscience 2022Quote: ... DRG were further lysate by sonicated (Vibra-Cell™, VWR) 5 times 10 secs.
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bioRxiv - Cell Biology 2020Quote: ... 3% glucose (VWR), 3.5% active dried yeast (SLS) ...
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bioRxiv - Synthetic Biology 2019Quote: ... the supernatant (containing secreted AAV virus) was collected and filtered through a 0.45-μm syringe filter (VWR). AAV virus was aliquoted into sterile Eppendorf tubes (0.5 mL/tube) ...
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bioRxiv - Microbiology 2022Quote: ... Virus titers were quantified and titrated by real-time PCR (QuantaBio ToughMix®, VWR International, Radner, PA) with a TCID50/ml equivalent as previously described (34) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were briefly sonicated using a Branson Sonifier 450 (VWR; output 4 ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mM MgCl2 (VWR), 0.1% NP-40 substitute (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lysates were transferred to Phase Lock Gel 2ml tubes (2302830; VWR). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were pulled down with either anti-HA Magnetic Beads (VWR PI88836) or IgG (Invitrogen MA5-14453 ...
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bioRxiv - Cell Biology 2020Quote: ... virus-containing supernatant was collected 48 h later and filtered through a 0.45 μm polyethersulfone filter (VWR 28145-505). For protein-coding constructs ...
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bioRxiv - Microbiology 2023Quote: ... The virus containing band was extracted from the gradient and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... G418 (VWR, 970-3-058), for several passages ...
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bioRxiv - Neuroscience 2022Quote: ... 3% Acetonitrile (VWR Cat#83640.320), 1 μg of LysC (Wako Cat#129-02541) ...
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bioRxiv - Neuroscience 2023Quote: ... and 3% hydrogen peroxide (VWR) for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... and 3 % of BSA (VWR) in PBS ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (which contains the majority of virus) was filtered through a 0.2 μM PES filter (VWR, cat. #97066-214). 25 mL of 40% PEG8000 (JT Baker ...
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bioRxiv - Bioengineering 2020Quote: ... 1-(3-(Dimethylamino)propyl)-3-ethylcarboiimide hydrochloride (EDC-HCL; 0.5 g, 2.6 mmol, 1.02 equiv., VWR) and NHS-ester (0.65 g ...
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bioRxiv - Cell Biology 2023Quote: ... covered with a glass plate (3" x 3" glass plate from VWR Vertical Gel Electrophoresis Systems), exposed to 7.5 minutes of glass plate filtered (UVA only ...
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bioRxiv - Immunology 2019Quote: ... Cell monolayers were processed for ICC staining at 24 to 72 hpi depending on virus replication kinetics (as previously described [94]) or stained with Giemsa stain (VWR; 35086). Relative plaque titre was calculated as the plaque titre of a virus stock under the indicated condition divided by its titre under a control condition ...
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bioRxiv - Genomics 2020Quote: ... The viscous lysate was transferred to a 2ml Phase Lock tube (VWR, Radnor, PA) and extracted twice with Tris-HCl-saturated phenol:chloroform:isoamyl alcohol (25:24:1 ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were passed over a Acrodisc PF syringe 0.8/0.2 μm filter (VWR) prior to incubation with HisPurTM cobalt resin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Whole cell lysates were obtained via mechanical lysis using a needle (VWR-TERUMO, AN2138R1) and RIPA cell lysis buffer (Sigma/Merck ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mM MnCl2 (VWR; Cat. # 1.05927.1000), 3 µM Na- orthovanadate (Sigma ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 (VWR, 97062-848) supplemented with 0.1% SUPERase•In™ RNase Inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Sf9 insect cells (VWR, #EM71104-3) were maintained in ExCell 420 serum-free media (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The cell lysates were completely transferred to polycarbonate tubes and centrifuged (Micro Star 30, VWR) at 25,000 × g ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were prepared with 6X SDS sample buffer containing 12% (v/v) β-ME (VWR) and separated on Bolt™ or NuPAGE 4-12% Bis-Tris gels (Invitrogen) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 3% Bovine Serum Albumin (VWR, #97061-416) in PBS ...
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bioRxiv - Systems Biology 2024Quote: ... in 3% BSA (VWR Cat# 97061-422) in PBS for 4 hours ...
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bioRxiv - Microbiology 2024Quote: ... on 3% agarose gel (VWR, Radnor, PA) with RedSafe Nucleic Acid Staining Solution (Bulldog Bio ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Neuroscience 2020Quote: ... the lysate was transferred to a 100 μm nylon cell strainer (VWR, ref nr 732-2759) to remove tissue debris and the flow-through was centrifuged at 2600 × g at 4 °C for 1 min to pellet nuclei ...
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bioRxiv - Evolutionary Biology 2019Quote: 1 ml of each lysate was first treated with 12.5 μl 1M MgCl2 (VWR Life Science), 0.4 μl DNAse 1 (2000 U/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were generated in ice cold RIPA lysis buffer (20mM Tris-HCl (VWR, 97061-258), pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... and washed/fixed with 3:1 methanol (VWR)/glacial-acetic acid (Carl Roth ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 15min washes in 5x SSCT (DAPI, VWR International ...
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bioRxiv - Microbiology 2020Quote: ... to 3 parts 0.5 M ammonia (VWR #BDH153312K) for 14 h ...
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bioRxiv - Physiology 2023Quote: ... Peroxidases were blocked by 3% hydrogen peroxide (VWR) in methanol (National Diagnostics ...
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bioRxiv - Developmental Biology 2023Quote: ... After fixing with 3:1 methanol (VWR 20846.326): glacial acetic acid (VWR 20102.292) ...