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Citations for VWR :
1 - 50 of 1155 citations for Dengue Virus Serotype 4 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Sf9 insect cells (VWR, #EM71104-3) were maintained in ExCell 420 serum-free media (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were resuspended in 50μl Schneider’s Insect Medium and transferred onto chambered cell culture slides (VWR, 53106-306). The chamber slides were pretreated with 0.25mg/ml Concanavalin A (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: Drosophila melanogaster S2R+ cells were maintained in Schneider’s Insect Medium supplemented with 10% heat inactivated FBS (VWR) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed overnight at 4 °C with 4% formaldehyde (VWR) in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Genetics 2020Quote: ... Cells were fixed using 4% PFA (VWR) for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were fixed with 4% formaldehyde (VWR) in PBS for 20 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Ovaries were dissected from 40 flies in Complete medium (Grace’s Insect Basal medium (VWR; #45000-476) supplemented with 15% FBS (ATCC ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were fixed with 4% formaldehyde (VWR Scientific) in PBS (RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were fixed in cold 4% paraformaldehyde (VWR) and stained with 0.5% crystal violet (VWR) ...
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bioRxiv - Bioengineering 2023Quote: Cell monolayers were fixed with 4% paraformaldehyde (VWR) for 15 min ...
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bioRxiv - Cell Biology 2021Quote: Cells were transfected with 4 µg DNA: 4 µL JetOPTIMUS transfection reagent (VWR, 76299-634) according to manufacturer guidelines in standard medium overnight ...
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 4% Formaldehyde (VWR, Cat# 100503) diluted in PBS ...
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bioRxiv - Bioengineering 2021Quote: ... cells were fixed using a 4% paraformaldehyde solution (VWR) for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were fixed with 4% Formaldehyde (VWR: 11699404) and stained with 0.05% crystal violet solution (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... A virus or tracer was injected using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Developmental Biology 2022Quote: ... tjTS>lglRNAi ovaries (with no UAS-tagged fluorescence reporter) were dissected in Grace’s Insect Basal medium (VWR; #45000-476), and incubated in Dihydroethidium (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were fixed with 4% of Formaldehyde (VWR: 11699404) and stained with 0.05% of crystal violet solution (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed in 4% paraformaldehyde (VWR, Radnor, PA, USA) at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were fixed with 4% paraformaldehyde (PFA, VWR, 100503-917), for 15 min at room temperature (RT ...
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bioRxiv - Physiology 2021Quote: 6xHis fusion proteins were grown in M15 cells (VWR) and prepared as above except that after IPTG induction ...
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bioRxiv - Biochemistry 2022Quote: ... Virus-containing supernatants were filtered (0.45 μm pore size cellulose-acetate filter (VWR)) ...
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bioRxiv - Microbiology 2019Quote: A549 cells were seeded on Nunc Lab-Tek 4-chamber (VWR) and inoculated with pneumococcal strains with a MOI of 50 as described above ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were fixed in cold 4% paraformaldehyde (VWR, Cat. # 101176-014) for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed overnight at 4 °C with 3.7% formaldehyde (VWR), 0.01 g/ml CaCl2.2H2O (Merck ...
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bioRxiv - Neuroscience 2020Quote: ... rabies virus was delivered by pressure injection (Nanoject II Variable Volume Automatic Injector, VWR) in 23 nl increments over a 3 min and 10 sec interval to a final volume of 500 nl ...
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bioRxiv - Developmental Biology 2020Quote: ... The pooled virus-containing supernatant was filtered through a 0.45 μM PES membrane (VWR). The filtered supernatant was then spun in an ultracentrifuge for 2 hours at 20,000 g at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... cells were plated at a density of 4×104 cells/cm2 in a 5-layer cell chamber (VWR 76045-402) to yield enough material for completing the experiment ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed overnight using 4% formaldehyde made in PBS (VWR, Sweden). The broad extended panel of antibodies used for staining are listed in Supplementary Table 1 ...
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bioRxiv - Immunology 2020Quote: Cell protein was isolated using RIPA lysis buffer (VWR, Ontario, Canada) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... The virus band was extracted and desalted on a NAP column (VWR, Cytiva, Illustra NAP) by elution in PBS ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... 24 hours post-transfection cells were fixed in 4% paraformaldehyde (VWR, AAJ61899-AK) for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed with ice cold 4 % paraformaldehyde (PFA)(43368.9M, VWR Stockholm Sweden) and for 15 min and permeabilized with 0.1 % (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... Multiple 1 l cell cultures in 4 l flasks (VWR cat # 32645–044) were incubated 72 h at 27 °C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed (4% formaldehyde, VWR International, in PBS; 10 min. at RT), washed (2 × 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were fixed with 4% formaldehyde stabilized with 0.5-1.5% methanol (VWR, 9713) for 12 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Equal volumes (20 μL) of the cell suspension and 4% trypan blue (VWR) were gently mixed ...
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bioRxiv - Neuroscience 2024Quote: ... We fixed the cells with 600 µL of 4% paraformaldehyde solution (VWR International) in PBS for 15 min and washed them with PBS three times ...
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bioRxiv - Bioengineering 2023Quote: ... the virus-particle-containing medium was collected and filtered using a 0.45 µm syringe filter (VWR), and then centrifuged at 25,000 × g for 90 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Protein cell lysates were collected by lysing and scraping cells with RIPA Lysis buffer (VWR, Mississauga, Ontario) mixed with protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... The dots were normalized on protein concentration (BCA assay) and cell numbers (EVE Automated Cell Counter, VWR). Each drop contained 10ug protein in 5 ul RIPA ...
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bioRxiv - Synthetic Biology 2019Quote: ... the supernatant (containing secreted AAV virus) was collected and filtered through a 0.45-μm syringe filter (VWR). AAV virus was aliquoted into sterile Eppendorf tubes (0.5 mL/tube) ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...