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Citations for VWR :
1 - 50 of 1122 citations for Dendritic Cell Specific Transmembrane Protein DCSTAMP Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Biophysics 2021Quote: ... biotin from VWR (Radnor, PA).
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bioRxiv - Microbiology 2024Quote: ... cell-specific MOI using serum-free Dulbecco’s modified Eagle’s medium (DMEM, VWR 45000-304) supplemented with 1x pen/strep ...
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bioRxiv - Microbiology 2024Quote: ... cell-specific MOI using serum-free Dulbecco’s modified Eagle’s medium (DMEM, VWR 45000-304) supplemented with 1x pen/strep ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were washed in 1x TBS-T and incubated for 1 hour at RT in the dark with species-specific secondary antibodies (Rb-680; 926-68071; Gt-680 925-68074, Ms-800 926-32212 Gt-800; 926-32214) (VWR) at 1:20,000 ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were washed in 1x TBS-T and incubated for 1 hour at RT in the dark with species-specific secondary antibodies (Rb-680; 926-68071; Ms-800 926-32212) (VWR) at 1:10,000 ...
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bioRxiv - Cell Biology 2024Quote: ... the coverslips were PEGylated by incubating in a 1:100 mixture of 1% biotin-PEG-silane in ethanol (Laysan Bio Biotin-PEG-SIL-2K-1g) and PEG-silane (85%, VWR 77035-498) for one hour in the dark at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Pan-flavivirus E protein antibody (1:100, VWR Enzo, 76285-044), GFAP (1:250 ...
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bioRxiv - Plant Biology 2022Quote: ... For biotin depletion with PD-10 desalting columns (VWR; 17085101), the columns were equilibrated with SDT:water (1:5 ...
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bioRxiv - Genomics 2024Quote: ... antibody overnight followed by incubation with protein A coupled Sepharose beads (VWR). Samples were washed with lysis buffer ...
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bioRxiv - Physiology 2021Quote: 6xHis fusion proteins were grown in M15 cells (VWR) and prepared as above except that after IPTG induction ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transformed with gene-specific linear DNA and selected on Kanamycin (50 µg/mL) (USP, VWR Life Science, USA) plates for gene deletion ...
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bioRxiv - Bioengineering 2021Quote: ... 100 µM of D-(+)-biotin (VWR, Mississauga, Canada; Cat#97061-446) and bacterial cell extract overexpressing E.coli BirA as described 81 ...
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bioRxiv - Molecular Biology 2020Quote: ... or a non-specific siGENOME Non-Targeting siRNA (Pool #2, Dharmacon) were transfected into U-2 OS cells with INTERFERin™ Polyplus Reagent (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Fragmented RNA was biotinylated using 10% MTSEA-biotin-XX (VWR, 89139-636) prepared in DMF ...
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bioRxiv - Immunology 2020Quote: Cell protein was isolated using RIPA lysis buffer (VWR, Ontario, Canada) supplemented with protease inhibitor cocktail (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were detected by immunoblotting using the following primary antibody concentrations in 5% milk (VWR)/Tris Buffered Saline -Tween20 (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Protein cell lysates were collected by lysing and scraping cells with RIPA Lysis buffer (VWR, Mississauga, Ontario) mixed with protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... The dots were normalized on protein concentration (BCA assay) and cell numbers (EVE Automated Cell Counter, VWR). Each drop contained 10ug protein in 5 ul RIPA ...
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bioRxiv - Systems Biology 2024Quote: ... Undissociated tissues were briefly sonicated in specific locations within a bath sonicator (VWR) to yield single-cell suspension.
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bioRxiv - Biophysics 2021Quote: ... Cell culture media growth factors and antibodies were purchased from VWR International PBI (Milan ...
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bioRxiv - Cancer Biology 2020Quote: ... Successively the endogenous peroxidases and non-specific binding sites were blocked using 3% H2O2 (VWR chemicals, 23615.248) and Protein-Block solution (DAKO Agilent technologies ...
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bioRxiv - Neuroscience 2024Quote: ... and sections from specific regions (Fig. 2A) were mounted on Superfrost Plus slides (VWR, West Chester, PA) coated with poly-D-lysine ...
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bioRxiv - Microbiology 2021Quote: ... Free biotin was removed by Macrosep Advance Spin Filter 3K MWCO 20 mL (Cat no. 89131-974, VWR, USA). Aliquots of the samples were stored at −80°C for use as input in western blot ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using a Bradford protein assay (VWR, E530-1L). In a 1.5mL tube ...
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bioRxiv - Molecular Biology 2024Quote: ... Specific differences relative to the published version included the following: (1) we added 0.1% Tween 20 (VWR 0777A) in the high-salt buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Immunology 2024Quote: ... Wild-type and MQIPQ-modified IL-10 protein variants were synthesised by transfecting HEK293T cells with pCCL_wtIL10-tNGFR or pCCL_MQIPQ-IL10-tNGFR (jetPRIME™; VWR) and collecting cell culture supernatants ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands were visualized using Blue BANDitTM protein stain (VWR Life Science, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... Samples were standardized for protein concentration using the Pierce BCA protein assay (VWR 23227), and incubated at 100L°C for 10Lmin under reducing conditions ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... cecal contents from specific-pathogen free (SPF) mice were resuspended in 10% weight/volume (w/v) Bacto Brain Heart Infusion (BHI) (VWR) supplemented with 0.05% w/v L-cysteine (Sigma ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated in a primary antibody (CD63 for the endosomal system, clone MX-49.129.5, VWR, anti-LAMP1 for lysosomes ...
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bioRxiv - Bioengineering 2021Quote: ... antibiotic-resistant colonies were re-streaked and verified by colony PCR using specific primers and Red Taq DNA polymerase master mix (VWR International).
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bioRxiv - Cancer Biology 2020Quote: ... the exons of interest in PD-L1 gene were amplified by using specific primers (Table 5) with a PCR thermal cycler (VWR Thermocycler, Leicestershire, England). PCR procedures were performed with a standard reaction mixture (4 μl/sample of Phusion GC Buffer (Thermo Fischer Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... These liver samples were homogenized with RIPA lysis buffer premixed with 1X protease and phosphatase-inhibitor cocktail and 1x EDTA (to prevent non-specific protease, metalloprotease, or phosphatase-mediated digestion) using a benchtop homogenizer (VWR VDI 12 Homogenizer). Samples were centrifuged at 4000 rpm for 10 minutes at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein was estimated using Bradford reagent (VWR).
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to BiotraceNT membrane (VWR) and incubated with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... and peqGOLD Protein-Marker I Unstained (VWR) were used as protein markers ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant protein extracts were recovered by centrifugation and protein concentration in samples was determined using Bradford Reagent (VWR).
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bioRxiv - Microbiology 2020Quote: ... Each fraction was resuspended in HEPES-NaCl buffer and 10 μL of each fraction was examined for protein concentration and lipid content using a Bradford protein assay (VWR) and the lipophilic FM4-64 dye (Molecular Probes) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... Protein concentration was estimated by Bradford reagent (VWR) using bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2021Quote: ... The protein was incubated overnight with TEV (VWR) at 4°C and Histag and TEV was purified out with Ni-NTA beads (GE) ...
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bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the proteins were diluted in 50 mM HEPES (VWR), pH 7.5 supplemented with 50 mM potassium sulphate (Honeywell ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: Protein extraction was performed with peqGOLD TriFast reagent (VWR), and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were precipitated with 20% trichloroacetic acid (TCA – VWR) on ice overnight at 4° C ...
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bioRxiv - Cell Biology 2022Quote: ... protein concentrations were measured using a Bradford kit (VWR). The lysate and supernatant samples were mixed with 1x Laemmli buffer with 1.5% 2-mercaptoethanol and were denatured at 95 ºC for 5 min ...