Labshake search
Citations for VWR :
51 - 100 of 106 citations for DNA Polymerase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: Genomic DNA was extracted using Direct PCR Lysis Reagent Cell (VWR). RNA was extracted using the AMBION PureLink RNA Mini Kit (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2022Quote: ... and total DNA content measured using the fluorometric Quantifluor dsDNA Assay (VWR) and converted to cell number for normalization.
-
bioRxiv - Cell Biology 2023Quote: ... DNA-RNA hybrids were eluted and treated with Proteinase K (VWR Scientific). Eluted DNA was subsequently isolated by phenol:chloroform:isoamyl alcohol extraction and DRIP-qPCR was performed on Myocardin promoter and control locus and results were calculated as per Sanz and Chédin ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were sorted into DNA LoBind® Eppendorf tubes (VWR, #525-0130), then CD45-cells were mixed with CD45+ cells at 2:1 to 10:1 ratios depending on cell availability to enrich for glioblastoma cells ...
-
bioRxiv - Microbiology 2024Quote: ... The precipitated DNA was resuspended in TE buffer (VWR, Radnor, PE, USA) and purified using QIAamp DNA Mini Kit (Qiagen) ...
-
bioRxiv - Molecular Biology 2020Quote: ... genomic DNA was extracted by lysing with DirectPCR Lysis Reagent Cell (Peqlab, VWR) supplemented with 0.5 μg/μl Proteinase K (Invitrogen) ...
-
bioRxiv - Microbiology 2020Quote: ... The DNA pellet was washed with 70% ethanol (VWR, Cat. No. 71006-012) twice and air dried ...
-
bioRxiv - Molecular Biology 2021Quote: ... DNA was precipitated by adding an equal volume of isopropanol (VWR, Radnor, PA) and incubated at −20°C for 15 min ...
-
bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from cortical tissue using the TriFast™ reagent protocol (VWR) and the Cacna1c gene was amplified with the PfuPlus! DNA Polymerase (Roboklon) ...
-
bioRxiv - Molecular Biology 2020Quote: ... DNA quality was examined via gel electrophoresis on a 0.5% agarose gel (VWR) with 0.1% GelRed (10000x ...
-
bioRxiv - Genomics 2020Quote: DNA was isolated by grinding with a disposable homogenizer pestle (VWR, Radnor PA, USA) on ice for 30 minutes followed by DNA extraction with the Qiagen DNeasy extraction kit (Qiagen ...
-
bioRxiv - Genetics 2019Quote: ... DNA was extracted from the gel slices using the peqGOLD Gel Extraction Kit (VWR). Genotyping was done using a modified ast-PCR protocol for R21X (Supplemental methods ...
-
bioRxiv - Biochemistry 2021Quote: ... The agarose was supplemented with peqGreen DNA/RNA dye (peqlab brand, VWR, Oslo, Norway) for visualization ...
-
bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA was amplified and purified using EZNA endo-free plasmid maxi kit (VWR International).
-
bioRxiv - Cell Biology 2021Quote: Cells were transfected with 4 µg DNA: 4 µL JetOPTIMUS transfection reagent (VWR, 76299-634) according to manufacturer guidelines in standard medium overnight ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA samples (1ug) were then separated on a 0.45% agarose gel (Biotechnology VWR, life science) at 30V 18-20 hours at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA was digested according to the peqGOLD DNase I Digest kit (VWR 13-1091-01), followed by washing steps with 400 µL RNA Prep Buffer (ZymoResearch R1060-2 ...
-
bioRxiv - Genetics 2023Quote: ... DNA was cleaned with phenol-chloroform phase separation with phase lock tubes (VWR, 10847-802). DNA was precipitated with 1 volume isopropanol ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The cell/DNA mix was then transferred to a pre-chilled 2 mm cuvette (VWR) and pulsed in a GenePulser Xcell (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... DNA samples (1ug) were then separated on a 0.45% agarose gel (Biotechnology VWR, life science) at 30V 18-20 hours in cold room ...
-
bioRxiv - Molecular Biology 2022Quote: ... We then transferred the column to a labeled DNA LoBind Eppendorf 1.5 mL tube (VWR # 022431021), added 20 uL nuclease-free H2O ...
-
bioRxiv - Genetics 2023Quote: ... DNA was cleaned and isolated with phenol-chloroform extraction using phase-lock tubes (VWR, 10847-802). 5 μg was run on a 1.5% agarose gel to ensure fragments of 200-400 bp were generated ...
-
bioRxiv - Biochemistry 2024Quote: ... Transient transfections were performed with JetPRIME DNA and siRNA transfection reagent (Polyplus supplied by VWR, 101000046).
-
bioRxiv - Evolutionary Biology 2019Quote: ... Phage DNA was extracted using an equal amount of phenol:chloroform:isoamyl alcohol (25:24:1) (VWR Life Science) to lysate ...
-
bioRxiv - Genomics 2022Quote: ... 1μL of unnormalized DNA was removed and transferred to a black 96-well plate (VWR, 33501-812) with 99μL of TE (10mM Tris ...
-
bioRxiv - Genomics 2023Quote: ... DNA was precipitated with isopropanol and stored in Tris-EDTA buffer at a pH of 8.0 (VWR). DNA purity was assessed via Nanodrop (Thermo Fisher Scientific ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... DNA concentration and purity were assessed with a spectrophotometer (NanoDrop® ND 1000, VWR International, Radnor, PA, USA). Library preparation and double-digest restriction site-associated DNA sequencing (ddRAD-seq ...
-
bioRxiv - Biochemistry 2021Quote: ... 400 ng DNA was transfected using 0.8 μl Jet Prime reagent in 40 μl Jet Prime buffer (VWR) per well ...
-
ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... The sheared DNA was then pre-cleared using magnetic beads (Sera-Mag Protein A/G SpeedBeads, VWR 17152104010150) for 1 hour to remove non-specific binding ...
-
bioRxiv - Genetics 2023Quote: ... DNA was counterstained with DAPI (0.2ug/mL) and the discs were mounted in Vectashield® (VWR, 101098-042) mounting media and imaged on a Leica Confocal SP8.
-
bioRxiv - Microbiology 2024Quote: Shotgun sequencing libraries were prepared using the Lucigen NxSeq AmpFREE Low DNA Library Kit (VWR International, Radnor, USA), and sequenced with PE150 on an Illumina NovaSeq6000.
-
bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
-
bioRxiv - Plant Biology 2019Quote: ... DNA fragments were separated in 2% (w/v) agarose gels and purified using the peqGOLD Gel Extraction Kit (VWR). Heat-shock transformation of chemically competent Escherichia coli DH5α cells were performed for all vectors followed by selection on LB-agar plates with ampicillin as a selection marker ...
-
bioRxiv - Bioengineering 2022Quote: ... 50/15 mL Falcon tubes and 50/5/2 mL DNA/Protein loBind Eppendorf tubes were purchased from VWR. D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen ...
-
bioRxiv - Genetics 2020Quote: Plasmid DNA containing the repair template with three tandem attP sites was co-transfected (1:1, Jetprime VWR#89129) with a plasmid containing Rosa26-Cas9/gRNA into mouse ES cells when confluency reached 70% ...
-
bioRxiv - Microbiology 2022Quote: ... All PCR products were run in 0.7% agarose gels using 100V for 50 min and visualized under blue light with EZ-Vision Bluelight DNA dye 10,000X (VWR). The thermocycling conditions for all PCRs were ...
-
bioRxiv - Microbiology 2023Quote: ... and adding sterile filter double distilled water (liquid medium) or autoclaved molten 2% DNA pure agarose (VWR, Radnor, PA) (agarose plates ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2 x 50 μL vaccine comprising DNA formulated in PBS was administered using Electroporation (EP) (BTX Agile pulse, VWR). In the remaining three immunizations ...
-
bioRxiv - Plant Biology 2023Quote: ... Quantitative and qualitative analysis of the isolated DNA was performed using a VWR mySPEC microvolume UV-visible spectrophotometer (VWR Lab Products Private Limited ...
-
bioRxiv - Molecular Biology 2023Quote: ... TGIRT was dissociated from the RNA-DNA duplexes by addition of 1 μL 5 M NaOH (VWR BDH7225-1) with incubation at 95 °C for 3 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... The quality of the DNA extracted was determined by electrophoresis using a 1.5 % agarose gel and with a spectrophotometer (VWR International) and quantified using the Qubit dsDNA HS Assay Kit (Life Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... Antibodies were produced by co-transfecting paired heavy- and light-chain antibody-expressing plasmids at a ratio of 1:1 using FectoPRO DNA Transfection Reagent (VWR) into Expi293 cells ...
-
bioRxiv - Microbiology 2020Quote: ... or 0.4 µM (cesA) of each primer (Table 3) and 60 ng of DNA (measured by a MySpec spectrophotometer, VWR International). qPCR reaction conditions for the panC detection followed the master mix supplier’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... Purification of Okazaki fragments was accomplished by running the denatured genomic DNA through 400 ul Source 15Q (VWR 89128-854), binding at 300 mM NaCl ...
-
bioRxiv - Cell Biology 2020Quote: ... dye and loaded onto a 1% agarose gel and as a ladder the 100 bp plus DNA ladder from VWR was used ...
-
bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
-
bioRxiv - Microbiology 2022Quote: ... and transfected with 800 ng/ml total DNA (including 50 ng/ml PA-X construct) using jetPRIME transfection reagent (Polyplus transfection, VWR). Cells were harvested 24 hours after transfection for RNA extraction and purification.
-
bioRxiv - Microbiology 2023Quote: ... Cells were transformed with gene-specific linear DNA and selected on Kanamycin (50 µg/mL) (USP, VWR Life Science, USA) plates for gene deletion ...
-
bioRxiv - Developmental Biology 2023Quote: ... We exposed the nucleic acids of each embryo with 5µl of QuickExtract™ DNA Extraction Solution (Lucigen, VWR, Philadelphia, PA), and incubated at 65°C for 15 minutes followed by 2 minutes at 98°C.
-
bioRxiv - Genomics 2020Quote: ... DNA was then reverse-crosslinked and purified using a standard phenol/chloroform cleanup method following the manufacturer’s protocol (VWR, cat. # VWRV0883). Secondary digestion of purified DNA was performed overnight at 37°C with 50 U of Csp6I (Thermo Scientific ...