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Citations for VWR :
1 - 50 of 52 citations for Cytomegalovirus Purified Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Antigen retrieval was performed by boiling slides in tris-buffered EDTA (VWR Chemicals, Amsterdam, the Netherlands) for 10 minutes in a microwave ...
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bioRxiv - Immunology 2022Quote: 3 µg/ml of spike antigens were coated onto the 384-well ELISA plates (VWR, Cat # 82051-300) overnight at 4 degree ...
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bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was performed by microwave boiling the slides in 10mM tri-Sodium Citrate buffer pH 6.0 (VWR). Aβ plaques were detected by staining with Thioflavin (SIGMA) ...
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bioRxiv - Cell Biology 2023Quote: ... temperature-mediated antigen retrieval was performed in citrate buffer (10 mM citric acid monohydrate (VWR, 20276.235, Pennsylvania, USA), Ph 6 ...
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bioRxiv - Microbiology 2020Quote: ... purified via PEG precipitation (25% polyethylene glycol 8000, VWR, USA), subsequently suspended in phage buffer (10 mM Tris-HCl ...
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bioRxiv - Bioengineering 2021Quote: ... and purified through a 0.45 um polyethersulfone filter (VWR #28143-505). Where required ...
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bioRxiv - Immunology 2022Quote: ... and the gB Domain II was purified with lectin resin (VWR.)
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bioRxiv - Genomics 2023Quote: DNA was purified using the yeast genomic DNA purification kit (VWR). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was purified using peqGOLD TriFast (VWR Peqlab; for UPF3B KO samples) or the Direct-zol RNA MiniPrep kit including the recommended DNase I treatment (Zymo Research ...
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bioRxiv - Bioengineering 2021Quote: ... and purified through a 0.45 um polyethersulfone filter from VWR (28143-505). Lentivirus was further concentrated via ultracentrifugation at 100,420 g for 90 min at 4°C in a Beckman Coulter Optima L-80 XP model and using a SW 41 Ti rotor ...
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bioRxiv - Zoology 2022Quote: ... purified using ExoCleanUp FAST PCR clean-up reagent (VWR Life Sciences USA), sequenced at the forward direction in outsourcing at StabVida (Portugal ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein samples were purified using HiPPR detergent removal resin (PI88305, VWR International) and dried completely by Speedvac (Savant ...
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A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPSbioRxiv - Bioengineering 2023Quote: ... The microgels were then purified by repeated washes with HEPES buffer (VWR), pH 8.3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were purified with Polyethersulfone (PES) 10kDa MWCO filters (VWR, 516-0230P) and Sep-Pack Vac C18 1cc/50mgv columns (Waters ...
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bioRxiv - Genetics 2023Quote: ... Labeled oligos were purified using MicroSpin G-25 Columns (VWR, #95017-621), then scintillation counts were performed using Liquid Scintillation Analyzer Tri-Carb 4910 TR (Perkin Elmer) ...
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bioRxiv - Genetics 2020Quote: ... pelleted and purified by the Plasmid Miniprep Kit I (peqGOLD, VWR, Radnor, USA). Inserts of the isolated plasmids were verified by sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... fluorescently labeled EV were purified using sepharose CL-2B (17-0140-01, VWR) size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2022Quote: ... mf are purified using a PD-10 Desalting Column (VWR, cat# 95017-001) into culture media and titered to a concentration of 10 mf/μL ...
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bioRxiv - Biophysics 2020Quote: Recombinant full-length human dynein was purified from Sf9 insect cells (#EM71104-3, VWR) using the multigene baculovirus expression system [38] as described previously [8] with few modifications ...
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bioRxiv - Microbiology 2022Quote: ... Dormant spores were purified by suspending the pellets in 20% Nycodenz (VWR, Atlanta, GA) followed by 50% Nycodenz density gradient centrifugation at 14000 g for 45 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... ribosome was purified using hydrophobic chromatography (3×5 mL HiTrap Butyl HP column (VWR)) with Fast Performance Liquid Chromatography (FPLC ...
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bioRxiv - Microbiology 2023Quote: UppSVU and UppSUN were purified from BL21 ArcticExpress DE3 RIL cells (VWR, Radnor, PA). Cells expressing pET-15b-UppSVU or pET-15b-UppSUN were grown in 10 mL LB containing carbenicillin overnight at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA was amplified and purified using EZNA endo-free plasmid maxi kit (VWR International).
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bioRxiv - Biochemistry 2024Quote: The protein was further purified by anion exchange chromatography (HiTrap Q, VWR 17-1154-01) with a linear gradient of increasing NaCl salt concentration ...
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bioRxiv - Physiology 2020Quote: ... Each library was diluted to 2nM with sterile purified commercially available molecular biology-grade water (VWR) and pooled in a multiplexed library sample ...
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bioRxiv - Immunology 2020Quote: 100,000 purified monocytes were cultured in 24-well tissue culture plates treated for increased attachment (VWR) in RPMI supplemented with 1% Human AB Serum (Omega Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... An equal volume (2.5 µl) of purified RNA was dotted on Amersham Hybond N+ membrane (VWR) and crosslinked using an UV Stratalinker 2400 (Autocrosslink mode) ...
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bioRxiv - Genetics 2021Quote: ... Amplified emulsion mixture was broken and purified by adding 1 mL of 2-butanol (VWR, AA43315-AK), 50 μL of AMPure XP SPRI (Beckman Coulter ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR) for cohesin trimers and a HiLoad 16/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR). Peak fractions were collected ...
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bioRxiv - Microbiology 2021Quote: ... purified cells were diluted and imaged at 100x magnification within a 20 μm counting chamber (VWR 15170-048). Images were analyzed using FIJI/ImageJ 1.52p (Java 1.8.0_172 ...
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments were separated in 2% (w/v) agarose gels and purified using the peqGOLD Gel Extraction Kit (VWR). Heat-shock transformation of chemically competent Escherichia coli DH5α cells were performed for all vectors followed by selection on LB-agar plates with ampicillin as a selection marker ...
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bioRxiv - Bioengineering 2021Quote: ... CQ loaded nanocarriers were purified from free CQ by 10K MWCO polyethersulfone membrane spin columns (VWR; Radnor, PA, USA) at 10,000 xg for 1-2 minutes and equilibrated with PBS solution.
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bioRxiv - Molecular Biology 2019Quote: ... Peak fractions were analyzed using SDS-PAGE and were further purified in a Superose 6 Increase 10/300 (VWR) using Buffer A as running buffer (free of EDTA/PMSF) ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... The norbornene-functionalized alginate product was purified by dialysis in a 10 kDa MWCO dialysis tube (Spectra/Por, regenerated cellulose, VWR) in 100 mM ...
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bioRxiv - Biophysics 2022Quote: ... The peak fractions containing SA1 were pooled concentrated to 1ml final volume and was purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR). Peak fractions were collected ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: 10 µg of purified RNA samples for each condition (wt and rie1 delete) was transferred to a PCR tube (VWR). Then 1.7 µl of primer homologous to IME1 was added to each tube ...
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bioRxiv - Cancer Biology 2023Quote: ... purified cells/cell aggregates were washed in PBS and frozen in 5% dimethyl sulphoxide (DMSO; 23486.297, VWR, Radnor, PA, USA) in FBS until xenografting.
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bioRxiv - Genomics 2020Quote: ... DNA was then reverse-crosslinked and purified using a standard phenol/chloroform cleanup method following the manufacturer’s protocol (VWR, cat. # VWRV0883). Secondary digestion of purified DNA was performed overnight at 37°C with 50 U of Csp6I (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... RNA was purified using 500 μl of Trizol reagent and precipitated from the aqueous phase with 500 μl of Isopropanol (VWR Medicals) and 1 μl of glycogen (20 μg/μl ...
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bioRxiv - Bioengineering 2021Quote: ... Dye-loaded nanocarriers were purified from free dye by dialysis or 10K MWCO polyethersulfone membrane spin columns (VWR; Radnor, PA, USA) at 10,000 xg for 1-2 minutes and equilibrated with PBS solution ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using prepGEM® Tissue kit according to a protocol adapted from the manufacturer (ZYGEPTI0500, ZyGEM, VWR International BV) and subjected to PCR using the primers listed in Supplemental Table S5 ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... and excess gel was washed away with 5 Milli-Q purified water washes and 5 Dulbecco’s Phosphate Buffered Saline (DPBS) solution (VWR L0119-0500) washes ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck, Germany). For this purpose ...