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Citations for VWR :
101 - 150 of 892 citations for Cow Polypyrimidine tract binding protein 1 PTBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... The sheared DNA was then pre-cleared using magnetic beads (Sera-Mag Protein A/G SpeedBeads, VWR 17152104010150) for 1 hour to remove non-specific binding ...
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Molecular Biology 2023Quote: ... then neutralized with 1 μL 5 M HCl (VWR BDH7419-1). The remainder of the library preparation was performed according to the xGen protocol except that post-ligation ...
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bioRxiv - Cancer Biology 2020Quote: ... DAB (Bright-DAB Substract Kit, IL ImmunoLogic, VWR) was used for protein detection ...
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bioRxiv - Microbiology 2023Quote: ... subtilis using the peqGOLD Bacterial DNA Kit (VWR). Plasmid DNA was isolated using the Nucleospin Extract Kit (Macherey-Nagel) ...
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bioRxiv - Biochemistry 2024Quote: ... peqGOLD Plasmid Miniprep Kit I (VWR Life Science). Successful mutagenesis was verified by Sanger sequencing.
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bioRxiv - Evolutionary Biology 2020Quote: ... and then 1 hour in 1:1 ratio of 30% sucrose/0.1M phosphate buffer:OCT (VWR, Cat No. 25608-930). Tissue was embedded inside a mold made by the severed end of a 1.5mL microcentrifuge tube attached to a glass slide with nail polish and stored at −80°C until used ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were prepared according to the manufacturer’s instructions and proteins were separated in 10 % Next Gel SDS-PAGE (VWR). Carbonylated proteins were detected with Immobilon Western Chemiluminescent HRP Substrate (MilliporeSigma).
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bioRxiv - Bioengineering 2022Quote: ... 50/15 mL Falcon tubes and 50/5/2 mL DNA/Protein loBind Eppendorf tubes were purchased from VWR. D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen ...
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bioRxiv - Immunology 2022Quote: ... The protein fraction was isolated by combining four times the volume of ice cold acetone (VWR Chemicals, PA, USA) to one volume of reconstituted PBMCsec ...
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bioRxiv - Systems Biology 2020Quote: ... Protein and crosslink digestion was carried out by adding 50 μL freshly prepared 20 mg/mL Proteinase K (VWR, freeze-dried powder suspended in 10 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cultured in 150mm dish (Alkali scientific TDN0150) for protein experiment and in 6-well culture plate (VWR, 100062-892) for total RNA experiment and transfected at around 50-60% confluency ...
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bioRxiv - Bioengineering 2023Quote: ... stably expressing enhanced green fluorescent protein (eGFP) were generated as described before[45] and cultured on 0.1% gelatin (VWR) pre-coated surfaces in MCDB-131 medium (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... and G418 sulfate (500 μg mL−1, from 50 mg mL−1 stock in H2O, from 712 U mg−1 powder, VWR). Cells were checked monthly for mycoplasma contamination by PCR assay ...
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bioRxiv - Developmental Biology 2023Quote: ... using the mouse MF20 (1:500, DSHB), the rabbit anti-GFP (1:1000, Life technology) and the rabbit RFP (1:1000, VWR) primary antibodies ...
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bioRxiv - Biophysics 2020Quote: ... 1% (w/w) 10:1 (base/crosslinker w/w) Sylgard-184 (VWR) was added to the GEL-8100 and mixed well for 60 seconds ...
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bioRxiv - Molecular Biology 2023Quote: ... Each sample was homogenised in 1 mol.L-1 HCl (VWR, United States). Free glucose content was measured at this stage ...
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bioRxiv - Immunology 2023Quote: ... HOIP (VWR/Proteintech 16298-1-AP, SHARPIN (VWR/Proteintech 14626-1-AP), and actin (Sigma-Aldrich A5316 ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was homogenized in 1 mol.L-1 HCl (VWR, United States) and free glucose content was measured ...
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bioRxiv - Genetics 2021Quote: ... 1 mM Squalene (VWR), 50 µM Lanosterol (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% BSA (421501J, VWR), 0.1% Triton-X and 0.05% Tween-20 in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM MgCl2 (VWR), 1% Triton X-100 (Alfa Aesar) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% penicillin-streptomycin (VWR, 5 units/mL penicillin ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM dithiothreitol (VWR) in 384-well plates (Corning ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM dithiothreitol (VWR) in 384-well plates (Corning ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... 1 cover glass (VWR) was used for the top plate and a 40 mm cover glass (Fisherbrand ...
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bioRxiv - Neuroscience 2023Quote: ... brains were kept for 1 hour in a 1:1 solution of 30% sucrose and Optimal Cutting Temperature Medium (OCT, VWR International) at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with the kit TaqPolimerase 2X (VWR) using the following PCR program ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Minipreps were performed using a peqGold miniprep kit (VWR). WMISH probes were generated via a SP6 or T7 transcription kit (NEB ...
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bioRxiv - Genomics 2024Quote: The peqGOLD Tissue DNA Mini Kit (VWR Life Science) was used to extract genomic DNA after genome editing in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The proteins were detected by adding chemiluminescence detection reagent (ECL Amersham) to the membranes and exposing onto ECL Hyperfilm (VWR) for 30m.
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bioRxiv - Biophysics 2020Quote: ... the untreated and treated bacteria (expressing H-NS-mEos3.2 fusion proteins) were filtered by 0.2 μm filters (VWR International LLC), resulting in filtrates constituting leaked cellular contents due to membrane damage ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... The dissected cuticle was sandwiched between two #1 coverslips (VWR #1 16004-094) with ~10 μL of UV curable resin (Bondic UV glue #SK8024 ...
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bioRxiv - Biochemistry 2024Quote: ... in a ratio of 1:10 in 1 mL of dichloromethanol (VWR BDH23373.400) and 50 μL of pyridine (Sigma-Aldrich #270970 ...
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bioRxiv - Cancer Biology 2023Quote: ... RPMI1640 complete media (1% FBS, 1× penicillin/streptomycin (VWR, Cat. No. CA45000-650), 1 μg/mL dexamethasone (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1 μg of either wild-type FHL-1 or RGD-null FHL-1 was incubated with 2 μg C3b (VWR International, Lutterworth, UK), and 0.4 μg factor I (FI ...
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bioRxiv - Neuroscience 2019Quote: ... serum-free culture media containing secreted proteins were collected and concentrated in 0.5 mL 3-kDa molecular weight cut-off centrifugal filters (VWR North America). In some experiments ...
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bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to a PVDF membrane for 1.5 H at 400 mA and blocked using 5 % BSA (VWR, 103219-864) rocking at room temperature for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... a 1:1 ratio of 0.6% agarose and 2X EMEM (VWR, Cat # 10128-758) supplemented with 5% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated 10 min (1:4000, stock 1 mg/ml, VWR, Vienna, Austria) followed by rinsing in PBS (three times 5 min) ...
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bioRxiv - Cancer Biology 2021Quote: ... or DMSO (VWR, 1:1000) as vehicle-control ...
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bioRxiv - Biochemistry 2019Quote: ... and 1% L-glutamine (VWR). All cells were incubated at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... 1 mM sodium pyruvate (VWR), 55 μM 2-mercaptoethanol (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% Triton X (VWR) for 1 hour ...