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Citations for VWR :
401 - 450 of 482 citations for Actin Alpha 2 Smooth Muscle ACTA2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% dextrose [VWR #90000– 904] ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii cultures were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
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bioRxiv - Synthetic Biology 2022Quote: Single and co-cultivation of (SbU-)-GFP and (SbU- + bts1Δ + thi6Δ)-mKate were cultured in 2 mL SC media YPD in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
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bioRxiv - Synthetic Biology 2022Quote: Single and co-cultivation of (SbU-)-GFP and (SbU- +bts1Δ + thi6Δ)-mKate were cultured in 2 mL SC media with and without thiamine in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 mL LB or BHI medium was inoculated with 20 µL preculture and grown in a 24-well plate (VWR, USA). Furthermore ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
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bioRxiv - Immunology 2023Quote: ... were cultured in six-well plates at a density of 5 x 106 cells/mL in 2 mL of RPMI 1640 (cat#45000-396, VWR, USA) supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii strains were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
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bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
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bioRxiv - Microbiology 2021Quote: ... and cleared refolded StaR was supplemented with additional 2 mM DTT and concentrated using a centrifugal filter with a MWCO of 3 kDa (VWR #516-0227P) and stored at −80 °C ...
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bioRxiv - Bioengineering 2021Quote: ... The cells were plated to ∼2 x 105 cells per well in a 6-well VWR tissue culture-treated plates (VWR, Suwanee, GA) and incubated ∼12 hours (overnight) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cell Biology 2021Quote: ... the eye cups were infiltrated with a 2:1 mixture of PBS containing 30% sucrose and OCT compound (VWR International, Radnor, PA) and frozen with dry ice ...
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bioRxiv - Biophysics 2021Quote: ... and subsequently incubated at 37 °C and 5% CO2 for 2 h before seeded and imaged on an 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Transformants were plated on SDCAA-agar (1.71 g/L YNB without amino acids and ammonium sulfate [Sigma-Aldrich #Y1251], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% dextrose [VWR #90000–904] ...
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bioRxiv - Bioengineering 2022Quote: ... a 3 mg/mL ORO stock was prepared by dissolving ORO powder in 100% 2-proponal (VWR #BDH2032-1GLP, Radnor, PA, USA). That stock solution was then diluted to a 1.8 mg/mL working solution in UltraPure™ Distilled Water (ThermoFisher #10977015) ...
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bioRxiv - Neuroscience 2019Quote: ... the flies were first sated with water and then presented with 100mM sucrose containing 2% blue dye (McCormick) filled in a graduated capillary (VWR, #53432-604). Until the flies became unresponsive to 10 serial food stimuli ...
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bioRxiv - Bioengineering 2021Quote: Plant or mammalian produced SARS-CoV-2 RBD protein were diluted in sterile 1X PBS to 2 μg/mL and coated onto a 96-well plates (VWR #62402-959) (50 μL /well ...
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bioRxiv - Microbiology 2022Quote: ... Spleen and brain were homogenized in 1 ml of sterile PBS followed by culture of 10-fold dilutions of each tissue on 2X GYE agar (2% glucose (VWR™, USA), 1% yeast extract (BD™ ...
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bioRxiv - Immunology 2023Quote: ... mice were whole body perfused through the heart with 2% paraformaldehyde (diluted down in PBS from 16%; VWR Cat. No. 15710-S), then livers were harvested and placed in 2% paraformaldehyde for 24 hours at 4°C then placed in 30% sucrose (Sigma-Aldrich Cat ...
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bioRxiv - Physiology 2023Quote: ... after overnight fasting followed by administration of a saline solution of glucose (2 g/kg or 1.5g/kg, for rats or mice, respectively; VWR Chemicals, Leuven, Belgium), by gavage ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed 3x for 15 minutes with PBS-T (PBS with 0.02% Tween) and incubated for 2 hours with either anti-rabbit HRP-linked (VWR, cat. no NA934) or anti-mouse HRP-linked (VWR ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Equal volumes (5 μL) of the resulting PCR product were loaded onto a 2% biotechnology grade Agarose I (VWR International, PA, USA) gel prepared with 1X TBE buffer and 0.0025% ethidium bromide ...
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bioRxiv - Neuroscience 2024Quote: ... mice were acclimated in a custom clear acrylic behavioral chamber (4” x 2” x 6”) with a filter paper bottom (VWR #28321-113) for 10 minutes prior to intranasal treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR, 75870-796) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 21°C in shaking culture in HL5 medium (Formedium, Norfolk, England) and on SM/5 agar plates (2 g/L glucose (VWR, Solon, OH), 2 g/L bacto peptone (Becton ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were probed with mouse anti-α-tubulin antibody (DM1A, VWR; #PI62204; 1:1000 in blocking buffer) for 1h at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... The labelled antibodies were dissociated from the beads by adding 15 μl 0.2M acetate (97064-482, VWR), pH 3.2 (elution buffer ...
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bioRxiv - Microbiology 2021Quote: ... 100 μL of HRP-labelled Rabbit Anti-Sheep IgG (H+L) secondary antibody (VWR, Batavia, IL, USA) diluted 1:1000 (100ng/mL ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with an antibody against RFP (1:1000, Rockland (VWR), catalog #RL600-401-379) ...
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bioRxiv - Systems Biology 2023Quote: ... during the secondary antibody staining and plates were sealed with adhesive foil (VWR Cat. No. 60941-124). Cells/nuclei were imaged on either an Opera Phenix high-content screening system (PerkinElmer ...
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bioRxiv - Plant Biology 2020Quote: Metabolite extraction was conducted by weighing out 25 mg of each freeze-dried peach mesocarp sample and placing them into clean 2 mL autosampler glass vials (VWR, Radnor, PA, USA). One mL of 80% by volume LC-MS grade methanol (MeOH ...
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bioRxiv - Systems Biology 2021Quote: Metabolite extraction was conducted by weighing out 19 - 21 mg of each freeze-dried sorghum roots and placing them into clean 2 mL autosampler glass vials (VWR, Radnor, PA, USA). Automated control of sample extraction (i.e. ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1 μg of either wild-type FHL-1 or RGD-null FHL-1 was incubated with 2 μg C3b (VWR International, Lutterworth, UK), and 0.4 μg factor I (FI ...
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bioRxiv - Cell Biology 2020Quote: ... Columns were washed with 2 ml MS grade water and eicosanoids were eluted with 500 µL methanol (MeOH abs.; VWR International, Vienna, Austria) containing 2% formic acid (FA ...
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bioRxiv - Biophysics 2022Quote: ... Cells were trypsinized and seeded on the substrate at a density of 30,000 cells cm-2 in a glass-bottom microscopy dish (VWR, 734-2906H, Barcelona, Spain) in chondrogenesis-inducing medium (ATCC ...
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bioRxiv - Biophysics 2022Quote: Before MS analysis, peptides were resuspended in loading buffer (2% ACN, 0.1% FA) and completely solubilized in a bath sonicator (VWR, 35 kHz, 10 min).
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bioRxiv - Microbiology 2023Quote: ... and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals, Dresden, Germany; ethylacetate, HiPerSolv Chromanorm, VWR Chemicals, Dresden, Germany). The suspensions were sonicated (5 min ...
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bioRxiv - Microbiology 2024Quote: ... a multi-element calibration standard containing each element was prepared using 1000 mg /L single-element standards (SPE Science, Canada) diluted with 2% (v/v) HNO3 (Aristar grade, VWR International, United Kingdom). The limits of detection for each element in He mode were 0.005 ...
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bioRxiv - Bioengineering 2024Quote: ... All dilutions of targets were performed in 4× PBS with 2 μM dT10 in GeneMate low-adhesion 1.7-mL microcentrifuge tubes (VWR, Cat No. 490003-230). PCR tubes containing target mixtures were then heated in a thermocycler to 73 °C for 3 min ...
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bioRxiv - Neuroscience 2023Quote: ... the substrates and products were visualized under a UV lamp at 254 nm and placed separately in vials in 2 ml of Scintran (VWR, Radnor, PA, USA). The radioactivity in disintegrations per minute (dpm ...
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bioRxiv - Immunology 2021Quote: Human IgM antibodies were detected in sera from passively transferred mice using high-binding 384-well plates (VWR) coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... Alkaline phosphatase coupled secondary antibodies were used for all blots followed by direct detection with NBT/BCIP (VWR) substrates on PVDF membranes.
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bioRxiv - Physiology 2020Quote: ... with bound antibodies eluted using glycine elution buffer [6.3 ml of 100 mM glycine (VWR Chemicals, Leicestershire, UK) and 0.7 ml of Tris (1M ...
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bioRxiv - Microbiology 2022Quote: ... Biofilms were incubated with anti-Candida antibodies diluted into dilution buffer (PBS with 0.05% Tween (VWR M147-1L), 0.1% BSA ...
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bioRxiv - Bioengineering 2023Quote: ... primary antibodies were added to the blocking solution for 20 minutes while rocking (VWR analog rocker 10127-876), in the dark and on ice ...
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bioRxiv - Biophysics 2021Quote: ... the coverslips/slides were placed in a 1% aminosilane solution (94 mL methanol, 1 mL 3-(2-aminoethylamino)propyltrimethoxysilane (VWR, CAS# 1760-24-3), 5 mL glacial acetic acid ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were then split into a negative control (no antibiotic) or antibiotic treatment (2 mL of culture per each treatment, using plastic Falcon tubes, VWR® Cat. No. 352059). After addition of the antibiotic from concentrated stocks ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μL cell suspension was loaded on YES agarose pad (2% agarose) and covered with 22 x 22 mm glass coverslip (VWR #631-0125, thickness: 1.5). Time-lapse imaging of mNG-cdc8 cells were performed at 30 °C with acquisition of 16 z-slices ...