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Citations for VWR :
601 - 650 of 1469 citations for 7 Chloro 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... or 0.4 µM (cesA) of each primer (Table 3) and 60 ng of DNA (measured by a MySpec spectrophotometer, VWR International). qPCR reaction conditions for the panC detection followed the master mix supplier’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with PBS 3 times and fixed and permeabilized with 3.7% formaldehyde and 0.3% Triton-X (VWR 80503-490) in PBS for 30 minutes at room temperature ...
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bioRxiv - Genetics 2022Quote: ... Pre-denaturation was performed at 91°C for 3 min and then 60°C for 20 min on heat blocks (VWR). After plates were spun ...
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bioRxiv - Genetics 2022Quote: ... and an equal volume of 1x Sperm Salts with 3% paraformaldehyde was applied for 5 min before samples were affixed to a Superfrost Plus slide (VWR). For both protocols ...
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bioRxiv - Genetics 2022Quote: ... Predenaturation was performed at 91 °C for 3 minutes and then 60 °C for 20 minutes on heat blocks (VWR). After plates were spun ...
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bioRxiv - Immunology 2020Quote: Wild-type (WT) and TLR4 knockout (KO) BMDMs were thawed and cultured for 3 days in non-Stissue culture treated dishes (VWR) in RPMI 1640 medium with L-Glutamine containing 10 % FBS and 10 % CM (= complete medium) ...
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bioRxiv - Physiology 2021Quote: ... 200 µl of these fixed RBCs were re-suspended in a blocking buffer containing 3% bovine serum albumin (VWR 0332) and 1% normal goat serum (Lampire Biological Laboratories 7332500 ...
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bioRxiv - Bioengineering 2022Quote: ... formulations were aliquoted 150 μL per well (n = 3/group) in a clear 96-well plate and sealed with optically clear and thermally stable seal (VWR). The plate was incubated in a microplate reader (BioTek SynergyH1 microplate reader ...
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bioRxiv - Genetics 2022Quote: ... A nonspecific antibody binding was prevented by an incubation of the fixed embryos in 3% BSA (VWR Life Science, 422351S) in PBS-T (1h ...
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bioRxiv - Developmental Biology 2023Quote: ... we aspirated ovarian follicles 3-8mm in diameter using an 18g needle (Single-Use Needles BD Medical, VWR, Philadelphia, PA) connected to a regulated vacuum system and collection bottle containing oocyte collection medium (OCM ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips coated with 0.1% gelatin were fixed for 20 minutes in 3% paraformaldehyde (VWR, Radnor, PA) (resuspended in PBS and brought to pH 8.0 with NaOH) ...
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bioRxiv - Molecular Biology 2023Quote: Monocytes were isolated as described above and 3×106 cells/ml were plated in a 6-well plate using RPMI-1640 medium (#392-0427, VWR) supplemented with 10% FBS (#11573397 ...
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bioRxiv - Cancer Biology 2023Quote: ... the upper part of the membrane was cleaned with cotton swab and lower part was stained with DAPI and the intensity of DAPI was measured using FlexStation 3 Multimode Plate Reader (VWR).
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bioRxiv - Bioengineering 2023Quote: ... 3% (w/v) alginate in in serum-free DMEM)and containing cells was placed in a Luerlock syringe (VWR, LOCA120006IM). 488mM calcium sulfate in serum-free DMEM was loaded into another Luerlock syringe and mixed with the cell-alginate solution through a Luerlock connector ...
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bioRxiv - Evolutionary Biology 2021Quote: ... which we then incubated at 25°C for 4 days prior to staining with iodine (VWR iodine crystals) vapor (Forsburg and Rhind 2006).
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bioRxiv - Cell Biology 2021Quote: ... mouse-keratinocyte co-cultures were washed twice with cold PBS and then fixed with 4% paraformaldehyde (VWR 1.04005.1000) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was kept overnight in a solution of PBS 0.01 mM/formaldehyde 4% (v/v; CAT#20909.330, VWR) and then cut in 50 μm slices with a vibratome (VT1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were then perfused with 30 mL of cold PBS and 4% paraformaldehyde (#100503-916, VWR, Radnor, PA) and were immediately decapitated to collect the brains ...
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bioRxiv - Developmental Biology 2021Quote: ... Fetuses and brains were then stored long-term at 4°C in 0.002% Sodium Azide (VWR - Bie&Berntsen) in PBS.
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bioRxiv - Molecular Biology 2022Quote: Cells were mounted on ∼1.4% w/v agar pads (in sterile ultrapure water) and a 0.13- to 0.17-mm glass coverslip (VWR) was placed on top ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were fixed with 4% PFA for 15 min and incubated with 40 mM Alizarin Red S (VWR, US) for 30 min ...
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bioRxiv - Physiology 2023Quote: ... a reversed-Phase C18 LiChroCART 250-4 column (LiChrospher 100 RP-18, 5 μm, VWR, Radnor, PA, USA) was used ...
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bioRxiv - Microbiology 2024Quote: ... infected HCT-8 cells in 96-well plates were fixed with sterile 4% paraformaldehyde (PFA; VWR Chemicals #28794.295) in PBS for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 5 Prime heavy 2 mL-phase lock gels (#2302830) were from VWR (Radnor, PA). High Capacity cDNA Reverse Transcription Kit (#4368814 ...
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Cell culture dimensionality influences mesenchymal stem cell fate through cadherin-2 and cadherin-11bioRxiv - Cell Biology 2019Quote: ... the mineralized extracellular matrix was stained with 2% (wt/vol) Alizarin Red S (VWR) solution in distilled water (pH 4.2 ...
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bioRxiv - Microbiology 2019Quote: ... the capillary was immobilized in the stainless-steel holder with 2 coverslips (N2, VWR) on the top to prevent bending upon contact with the objective ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were sonicated for 2 min on an ice-water bath (VWR Scientific) and then incubated at 60°C for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... The sample was added to a Phase-lock Gel Heavy 2 ml tube (VWR) and centrifuged at 12,000 × g for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with a blocking solution (2 % bovine serum albumin (BSA; VWR) diluted in PBS ...
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bioRxiv - Physiology 2023Quote: ... Homogenized tissue generated myofibrils using 2 x 30 sec pulses of (VWR Radnor, PA) at low speed and stored at 4 °C to be used for up to two days ...
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bioRxiv - Microbiology 2023Quote: ... before homogenizing in 2 ml sterile PBS using a hand-held tissue homogeniser (VWR). Ten-fold serial dilutions of tissue homogenates were then prepared and plated onto B ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered into 2 mL short thread vials with crimp caps (VWR International GmbH ...
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bioRxiv - Molecular Biology 2023Quote: ... After PCR amplification using NEBNext High-Fidelity 2 × PCR Master Mix (VWR 102500-096) and post-PCR cleanup using SPRI beads (Beckman Coulter A63880) ...
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bioRxiv - Microbiology 2023Quote: ... every 2 days or a cocktail of PGEs inhibitor (CAY10526 5 mg/kg VWR) and prostanoid receptor antagonist 2 (AH6809 5mg/kg Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was filtered into 2 mL short thread vials with crimp caps (VWR International GmbH ...
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bioRxiv - Plant Biology 2020Quote: 1-Aminocyclopropane-1-carboxylic acid (ACC; VWR P10007) was dissolved in sterile water (10 mM stock ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 (VWR) were cleaned by a 20 minute exposure in a 2.6 L Zepto plasma laboratory unit (Diener Electronic) ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with TBS-T (20 mM Tris-HCl, pH 7.6, 136 mM NaCl, 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1h ...
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bioRxiv - Neuroscience 2019Quote: ... serum-free culture media containing secreted proteins were collected and concentrated in 0.5 mL 3-kDa molecular weight cut-off centrifugal filters (VWR North America). In some experiments ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was blocked with TBS-T (20mM Tris-HCl, pH 7.6, 136mM NaCl and 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1 hour ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 minutes), once with 2X SSC (room temperature, 3 minutes) and then treated with 50 ug/mL of RNase A (VWR 1247C393) in 2X SSC for 45 minutes at 37°C by overlaying the sample with 500ul of RNAse solution and incubating in a humidified chamber ...
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bioRxiv - Neuroscience 2023Quote: ... for 50 ms at 950 ms intervals using electrodes (diameter 3 mm; Sonidel CUY650P3) and ECM-830 BTX square wave electroporator (VWR international). After electroporation ...
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bioRxiv - Biochemistry 2023Quote: ... whereas other enzymes were removed by passing the sample through a 3 kDa NanoSep centrifugal filter device (VWR, Radnor, PA, USA).
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bioRxiv - Biochemistry 2024Quote: ... 40 mM imidazole and 200 mM NaCl was added before loading on a self-packed 3 ml Ni Sepharose™ 6 Fast Flow (VWR) column using ÄKTA Pure FPLC system (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... and Matrigel Basement Membrane Matrix (1:1) (354234, VWR).
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bioRxiv - Cancer Biology 2021Quote: ... Cell supernatants were collected at different time points and centrifuged 30min at 10,000 × g at 4°C to eliminate cell debris (centrifuge CT15RE, VWR), then at 100,000 × g for 3h to eliminate larger vesicles (L7-55 Ultracentrifuge ...
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bioRxiv - Immunology 2020Quote: These assays were done using methods similar to those described (52) and Immulon 4 microtiter plates (VWR, Radnor, PA) coated with 100ng per well of S1 ...
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bioRxiv - Microbiology 2022Quote: ... Brains and lungs were collected at 4-days post inoculation and homogenized in PBS with a benchtop homogenizer (VWR). Homogenates were diluted in a 10-fold dilution series and each dilution was plated on YPD ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were euthanized with euthasol and transcardially perfused with 0.3% glutaraldehyde-4% paraformaldehyde in PBS (0.1 M, pH 7.4; VWR Chemicals, France), and the neurobiotin-filled neurons revealed as previously described (Williams et al. ...
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bioRxiv - Immunology 2023Quote: ... then placed overnight at 4°C in a 25% sucrose solution containing 50% optimal cutting temperature compound (OCT, VWR Clear Frozen Section Compound ...