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Citations for VWR :
551 - 600 of 1194 citations for 7 8 Dihydro 6H 1 6naphthyridin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... Transformants were plated on SDCAA-agar (1.71 g/L YNB without amino acids and ammonium sulfate [Sigma-Aldrich #Y1251], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% dextrose [VWR #90000–904] ...
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bioRxiv - Synthetic Biology 2019Quote: ... Culture slants were made by pouring approximately 5-10 mL of PDYA at an angle into a 15 mL Falcon tube (VWR, Radnor, PA) and allowing the PDYA to solidify along the inner sidewall of the tube ...
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bioRxiv - Genomics 2021Quote: ... and myotubes from one 10 cm plate (∼5 million cells) with >90% viability were lifted as described above and pelleted in 15 mL polypropylene falcon tubes (VWR, 89039-670) by centrifuging for 5 minutes at 1500 RPM ...
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bioRxiv - Physiology 2020Quote: ... 5-μm sections were stained with hematoxylin and eosin (H&E) using the Leica Autostainer and mounted in DePeX (VWR, Lutterworth, UK). Adipocyte diameter and pancreatic β-cell islet number and size were quantified using ImageJ software as previously described (7 ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA/DNA hybrid was tagmented in TD reaction buffer (10 mM Tris-Cl pH 7.6, 5 mM MgCl2, 10% DMF) supplemented with 3.4% PEG8000 (VWR Life Science, Cat.No.97061), 1 mM ATP (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... decalcification solution for 14 days and then paraffin embedded for sectioning with a Leica RM2235 microtome at 5 μm per section to generate histology slides (VWR 631-1553). Sections were processed and stained with TRAP solution and counterstained with 0.2% methyl green solution (Bioenno Lifesciences ...
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bioRxiv - Biochemistry 2022Quote: ... Individual yeast colonies were picked into 4mL of pH 4.5 Sabouraud Dextrose Casamino Acid media (SDCAA: Components per liter - 20 g dextrose, 6.7 grams yeast nitrogen base (VWR Scientific, Radnor, PA), 5 g Casamino Acids (VWR) ...
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bioRxiv - Biochemistry 2022Quote: The eluates of SILAC “heavy” and “light” were combined for each SA+ and SA-group respectively followed by reduction in 5 mM TCEP (VWR, #97064-848) for 1 hour and alkylation in 10 mM iodoacetamide (VWR ...
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bioRxiv - Biophysics 2022Quote: ... peptides were resuspended in 20 μL of resuspension buffer (5:95 ACN:MilliQ H2O, 0.1% TFA) and solubilized in a bath sonicator (VWR, 35 kHz, 10 min). A ZipTip was placed on a P10 pipettor set to 10 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-2 preps of in vitro transcribed RNA and 5-10µg N gene RNA were mixed with the prepared cell suspension in a 4mm electroporation cuvette (VWR International # 89047-210) and pulsed in a BioRad GenePusler using the exponential protocol with the following settings ...
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bioRxiv - Microbiology 2022Quote: ... containing 1.5 ml of brain–heart infusion broth ([BHI]; Becton Dickinson, Franklin Lakes, NJ) supplemented with 0.1% Tween 80 (VWR Chemicals, Solon, OH) and were incubated at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were put into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). The columns were equilibrated in high salt buffer (20mM Tris-HCL ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then loaded into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). Once the sample completely flowed through the column ...
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bioRxiv - Neuroscience 2023Quote: ... The PVDF membranes were blocked in 5% Nonfat dry milk (Bio- Rad Laboratories, Cat.# 1706404) in Tris-buffered saline with 0.1% Tween 20 (TBST) (VWR, Cat.# K873-4L). Samples were probed with the primary antibodies listed in Supplemental Table 1 overnight in 5% bovine serum albumin (Gold Biotechnology ...
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bioRxiv - Genetics 2023Quote: ... 500μl of concentrated bacterial suspension or 5% (w/v) sucrose solution (mock treatment) was added to empty fly vials containing a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Biophysics 2023Quote: ... The 40 μL solution of the lipid mixture was spread on a 5% w/v PVA film dried on a 25 x 25 mm coverslip (VWR, Radnor, PA) and then put under vacuum for at least 2 h to form a dry lipid film ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 21°C in shaking culture in HL5 medium (Formedium, Norfolk, England) and on SM/5 agar plates (2 g/L glucose (VWR, Solon, OH), 2 g/L bacto peptone (Becton ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Equal volumes (5 μL) of the resulting PCR product were loaded onto a 2% biotechnology grade Agarose I (VWR International, PA, USA) gel prepared with 1X TBE buffer and 0.0025% ethidium bromide ...
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bioRxiv - Cancer Biology 2022Quote: ... and serially diluted 1:1 in fresh medium using a 12-well dilution reservoir (VWR) to achieve 10 drug concentrations (including one untreated) ...
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bioRxiv - Immunology 2024Quote: ... then transferred to a 1:1 solution of 30% sucrose and OCT (VWR, 95057-838) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Concentration and quality of total RNA isolations using a phenol-chloroform extraction with Isol-RNA lysis reagent (5’Prime, Hamburg, Germany) from endothelial cells were verified by a NanoDrop™ 1000 (Peqlab VWR, Radnor, PA) and an Agilent 2100 bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... the exons of interest in PD-L1 gene were amplified by using specific primers (Table 5) with a PCR thermal cycler (VWR Thermocycler, Leicestershire, England). PCR procedures were performed with a standard reaction mixture (4 μl/sample of Phusion GC Buffer (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2020Quote: Leaf tissue was collected 7 days post infection, transferred to an extraction bag (Bioreba, Switzerland) and mixed with 5 ml of phosphate buffered saline (VWR, West Chester, PA), hand homogenized using a hand-held tissue homogenizer (Agdia ...
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bioRxiv - Molecular Biology 2021Quote: ... or 5% (w/v) non-fat dry milk in TRIS-buffered saline (TBS) containing 0.1% Tween-20 (TBS-T; VWR, Radnor, PA, USA, # 28829.183) for one hour at room temperature and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... to a 50-mL Falcon tube on ice and homogenized at a speed of ∼1000 rpm for 5 sec to brake nuclei clumps with a handheld homogenizer (VWR® 200 Homogenizer). After homogenization ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals, Dresden, Germany; ethylacetate, HiPerSolv Chromanorm, VWR Chemicals, Dresden, Germany). The suspensions were sonicated (5 min ...
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bioRxiv - Neuroscience 2024Quote: ... 12 µg of plasmid DNA (ratio 1:10 for guide-RNA and dCas9-construct containing plasmids) was diluted into 5 µl of sterile 10 % glucose and added to diluted In vivo-jetPEI® reagent (#101000040 VWR, Polyplus-transfection). The solution was mixed by pipetting and incubated at RT for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... and the spores were resuspended in 5 ml of 50% ethanol and incubated at 4°C in a tube rotator (VWR Mini Tube Rotator) running at 15 rpm for 12 h at room temperature to inactivate remaining vegetative cells ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mM ethylenediaminetetraacetic acid (EDTA, VWR) in autoclaved H2O ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1% (v/v) penicillin-streptomycin (VWR, 5 units/mL of penicillin and 5μg/mL streptomycin) ...
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bioRxiv - Biochemistry 2019Quote: ... 1% non-essential amino acids (VWR) and 1% L-glutamine (VWR) ...
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bioRxiv - Genetics 2021Quote: ... 1 mM DTT (VWR 97061-340), and stored at −20°C ...
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bioRxiv - Physiology 2021Quote: ... 1% non-essential amino acids (VWR) and 1% L-glutamine (VWR) ...
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bioRxiv - Plant Biology 2021Quote: ... 1% NP-40 (VWR Life Science), 1 mM PMSF (GoldBio) ...
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bioRxiv - Cell Biology 2020Quote: ... which includes 1 mM ATP (VWR), 1 mg/ml creatine phosphate (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... IFT88 (1:500) (VWR; 10088-640), NPHP4 (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... CP110 (1:100) (VWR; 76045-052), IFT88 (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... passed in 1% acetic acid (VWR) for 2 min ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 g/L glucose (VWR, US) and 1 g/L Bovine Serum Albumin (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 1 uL of 20X Evagreen (VWR), 2 uL each of 5 uM forward and reverse primers (519F ...
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bioRxiv - Neuroscience 2020Quote: ... 30000 min-1 (VDI 12, VWR). NAD concentration was performed using a bioluminescent assay (NAD/NADH Glo-™ ...
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bioRxiv - Bioengineering 2020Quote: ... and 1 mM EDTA (VWR international). Folded structures were purified and concentrated by using Amicon 100K filter tube (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... with DAPI (VWR, IC15757410; 1:1000). Slides were kept at RT overnight before moving to 4°C for long-term storage ...
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bioRxiv - Cell Biology 2021Quote: ... Cep290 (1:100) (VWR; 10084-648), Rootletin (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... NPHP4 (1:100) (VWR; 10091-250), Cep290 (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... and anti-mouse (1:10,000; VWR)-conjugated to horseradish peroxidase HRP.
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bioRxiv - Cancer Biology 2023Quote: ... 1% v/v penicillin-Streptomycin (VWR), 0.5 ng/mL of beta-estradiol (US Biological) ...
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bioRxiv - Bioengineering 2023Quote: ... 1 glass coverslips (#48404-097, VWR). ECs (6 million mL-1) ...
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bioRxiv - Cell Biology 2023Quote: ... 1% v/v Penicillin/Streptomycin(VWR), 3.5µL/L β-mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: ... 1% v/v MEM-NEAA(VWR), 1% v/v Glutamax(Gibco ...