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Citations for VWR :
451 - 500 of 557 citations for 6 methoxypyridine 3 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... a reversed-Phase C18 LiChroCART 250-4 column (LiChrospher 100 RP-18, 5 μm, VWR, Radnor, PA, USA) was used ...
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bioRxiv - Microbiology 2024Quote: ... infected HCT-8 cells in 96-well plates were fixed with sterile 4% paraformaldehyde (PFA; VWR Chemicals #28794.295) in PBS for 10 minutes at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... INS-1 β-cells were first fixed in 4% paraformaldehyde (PFA) in PBS solution (VWR, Cat# ALFAJ61899.AK) for 30 min ...
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bioRxiv - Immunology 2020Quote: RNA was extracted using a modified phenol-chloroform protocol (Chomczynski and Sacchi, 2006) with 1-Bromo-3-chloropropane and Isopropanol (Sigma and VWR, respectively). Total RNA was quantified and assessed for quality and integrity by Bioanalyser (RNA Pico 6000 Chip ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with TBS-T (20 mM Tris-HCl, pH 7.6, 136 mM NaCl, 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1h ...
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bioRxiv - Neuroscience 2019Quote: ... serum-free culture media containing secreted proteins were collected and concentrated in 0.5 mL 3-kDa molecular weight cut-off centrifugal filters (VWR North America). In some experiments ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was blocked with TBS-T (20mM Tris-HCl, pH 7.6, 136mM NaCl and 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1 hour ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 minutes), once with 2X SSC (room temperature, 3 minutes) and then treated with 50 ug/mL of RNase A (VWR 1247C393) in 2X SSC for 45 minutes at 37°C by overlaying the sample with 500ul of RNAse solution and incubating in a humidified chamber ...
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bioRxiv - Immunology 2021Quote: ... A single incision was made above the quadriceps and a 2 mm or 3 mm biopsy punch (VWR, 21909-132, −136) was used to make a fullthickness muscle defect ...
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bioRxiv - Neuroscience 2023Quote: ... for 50 ms at 950 ms intervals using electrodes (diameter 3 mm; Sonidel CUY650P3) and ECM-830 BTX square wave electroporator (VWR international). After electroporation ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... whereas other enzymes were removed by passing the sample through a 3 kDa NanoSep centrifugal filter device (VWR, Radnor, PA, USA).
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bioRxiv - Developmental Biology 2023Quote: ... for 50 ms at 1 s intervals using electrodes (diameter 3 mm; Sonidel CUY650P3) and ECM-830 BTX square wave electroporator (VWR international). After electroporation ...
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bioRxiv - Molecular Biology 2021Quote: ... and the samples were vortexed 4 × 20 seconds at full speed on a vortex Genie 2 (VWR Scientific Products). The cellular debris were removed by centrifugation at 2,600g for 5 min at 4°C and 1.5 ml of the lysate was transferred to a micro-tube and centrifuged again at 9500 g for 5 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and sequentially washed with RIPA (PBS pH 7.4 with 0.5% sodium deoxycholate [Thermo], 0.1% sodium dodecylsulfate [Fisher Scientific, Waltham, MA], 1% Nonidet P-40 substitute [VWR]), high salt PBS (PBS pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell supernatants were collected at different time points and centrifuged 30min at 10,000 × g at 4°C to eliminate cell debris (centrifuge CT15RE, VWR), then at 100,000 × g for 3h to eliminate larger vesicles (L7-55 Ultracentrifuge ...
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bioRxiv - Immunology 2020Quote: These assays were done using methods similar to those described (52) and Immulon 4 microtiter plates (VWR, Radnor, PA) coated with 100ng per well of S1 ...
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bioRxiv - Microbiology 2022Quote: ... Brains and lungs were collected at 4-days post inoculation and homogenized in PBS with a benchtop homogenizer (VWR). Homogenates were diluted in a 10-fold dilution series and each dilution was plated on YPD ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were euthanized with euthasol and transcardially perfused with 0.3% glutaraldehyde-4% paraformaldehyde in PBS (0.1 M, pH 7.4; VWR Chemicals, France), and the neurobiotin-filled neurons revealed as previously described (Williams et al. ...
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bioRxiv - Immunology 2023Quote: ... then placed overnight at 4°C in a 25% sucrose solution containing 50% optimal cutting temperature compound (OCT, VWR Clear Frozen Section Compound ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were collected onto copper mesh grids and contrasted for 2min with 4% uranyl acetate solution in methanol (VWR), followed by 2min in lead citrate (Reynold’s solution) ...
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bioRxiv - Immunology 2024Quote: ... in Ce3D using Frame Seal incubation chambers (Bio Rad) and kept at 4°C in the dark after coverslipping (thickness no. 1.5; VWR). Slices were imaged on a Zeiss LSM 880 confocal microscope using a 32 channel Gallium arsenide phosphide (GaAsP ...
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Synaptonemal Complex dimerization regulates chromosome alignment and crossover patterning in meiosisbioRxiv - Cell Biology 2020Quote: ... pSUN-1::TIR-1::mRuby him-8(tm611) spo-11-aid IV (ROG212) adult hermaphrodites were grown on 1 mM auxin (indole-3-acetic acid, VWR AAA10556-36) and therefore lacked SPO-11 ...
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bioRxiv - Bioengineering 2022Quote: ... centrifuged for 3 minutes at 300 g and resuspended in E8 flex medium containing 10 μM Y27632 ROCK inhibitor (VWR, #688000-5). Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning ...
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bioRxiv - Biochemistry 2019Quote: ... E1A alternatively spliced isoforms were amplified from 3 μl cDNA by PCR in 50 μl reactions with the KAPA HiFi PCR Kit (Peqlab/VWR, Darmstadt, Germany) using primers E1A_ Exon1_forward 5’-GTT TTC TCC TCC GAG CCG CTC CGA-3’ and E1A_Exon2_reverse 5’-CTC AGG CTC AGG TTC AGA CAC AGG-3’ at final concentrations of 500 nM ...
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bioRxiv - Microbiology 2021Quote: ... and cleared refolded StaR was supplemented with additional 2 mM DTT and concentrated using a centrifugal filter with a MWCO of 3 kDa (VWR #516-0227P) and stored at −80 °C ...
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bioRxiv - Biochemistry 2020Quote: ... samples were washed for 3×5 minutes in PBSM and permeabilized for 10 minutes in PBSM + 5 mg/mL BSA (VWR VWRV0332-25G) + 0.1% Triton-X100 (Sigma-Aldrich T8787-100mL) ...
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bioRxiv - Microbiology 2021Quote: ... enterocolitica samples were eluted by washing the wells 3 times with warm PBS followed by treatment with 500 μl PBS with 1% Triton X-100 (AMRESCO, VWR, Randor, PA) for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... a 3 mg/mL ORO stock was prepared by dissolving ORO powder in 100% 2-proponal (VWR #BDH2032-1GLP, Radnor, PA, USA). That stock solution was then diluted to a 1.8 mg/mL working solution in UltraPure™ Distilled Water (ThermoFisher #10977015) ...
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bioRxiv - Neuroscience 2019Quote: ... the aggregates were washed 3× in a 15-ml tube by sedimentation in 3:1 medium and then maintained in suspension on sterile petri dishes (VWR, #391‒2016) in 10 – 15 ml 3:1 medium with media changes every other day ...
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bioRxiv - Cell Biology 2022Quote: ... Animals were then homogenized 20x in 3 mL of M9 buffer using an ice-cold 15 mL Dura-Grind Stainless Steel Dounce Tissue Grinder (VWR, 62400-686). Crude lysate was transferred to 1.5 mL tubes and frozen in liquid N2.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 2% FBS were injected into the left ventricle of the mice with a 26G × 3/8” needle attached to tuberculin syringe (VWR, Cat: BD309625). During the course of colonization assays ...
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bioRxiv - Microbiology 2020Quote: ... concentrated OMVs were diluted 4 times in dH2O and 5 μL of this dilution was added to 150 μL Bradford reagent (VWR). Absorbance was measured at 595 nm and compared to a standard curve to calculate protein concentration ...
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bioRxiv - Cell Biology 2020Quote: ... After 1h binding on a wheel at 4°C beads were washed 3x in PBS supplemented with 0.02% Tween20 (VWR, M147) and 1% SDS and eluted with NuPAGE LDS buffer supplemented with 50 mM DTT and 1 mM biotin at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were centrifuged at 14000 rpm at 4°C and the supernatants clarified with a 0.2 μm membrane syringe filter (VWR International) before proceeding with a two-step purification protocol ...
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bioRxiv - Microbiology 2019Quote: Colony morphology agar was prepared as described above and 4 mL of the mixture were poured into 35 mm×10 mm round Petri dishes (25373-041, VWR)and left to cool and solidify overnight (14 to 24 h ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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bioRxiv - Biophysics 2019Quote: ... (4) four times BRB40 buffer (40 mM Pipes [Sigma], pH 6.84, with KOH [VWR], 1mM EGTA [Sigma], 4mM MgCl2 [VWR]) to remove excess F-127 in solution and exchange buffers ...
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bioRxiv - Biochemistry 2020Quote: Frozen hippocampus brain tissues were homogenized in a 4 volume of the 10% trichloroacetic acid (VWR International, Radnor, PA, USA) solution using a Kontes glass tissue grinder ...
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bioRxiv - Biophysics 2021Quote: ... cells were plated at a density of 4×104 cells/cm2 in a 5-layer cell chamber (VWR 76045-402) to yield enough material for completing the experiment ...
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bioRxiv - Microbiology 2020Quote: ... and 1 volume of phenol (pH 4.3):chloroform (1:1), followed by centrifugation (16000 x g, 4 °C, 10 min) in a phase lock tube (Quantbio, VWR) to separate the aqueous and organic phases ...
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bioRxiv - Genomics 2021Quote: Tissue sections were prepared from formalin-fixed paraffin embedded tissue blocks and cut to 4 micrometers serial sections and mounted on Superfrost Plus (VWR). The procedure for multiplex immunohistochemistry (mIHC ...
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bioRxiv - Physiology 2021Quote: ... on a shaker at 4°C in tris-buffered saline with 1% tween 20 (TBS-T; VWR Amresco ProPure M147) and 0.1 g ml-1 skim milk powder (Kroger ...
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bioRxiv - Plant Biology 2021Quote: ... one of tissue piece (50 mg) was ground using a 4-mm steel ball in a 2-ml Eppendorf tube in a bead beater (VWR) for 1 min at 30 revolutions·s−1 ...
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bioRxiv - Biophysics 2020Quote: ... The cell lysate was separated from insoluble material by centrifugation at 40,000 g for 60 minutes at 4 °C and subsequently incubated with a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C on a rotator for affinity chromatography via the 6xHis-tag ...
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bioRxiv - Plant Biology 2022Quote: ... 0.4 g of poly(FATRIFE-ter-PEGMA-ter-NBMA) terpolymer was dissolved in 25ml methyl-ethyl ketone (25642.325, VWR, UK) in a 500 ml rotary evaporator flask containing 20 g of FEP at 40 °C to coat FEP particles ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated at 4℃ to maintain their viability and passed through a 40 µm cell strainer (VWR, 76327-098) to dissociate single cells from clumps ...
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bioRxiv - Biochemistry 2019Quote: ... microalgae were harvested via centrifugation at 13G (9000 rpm) for 20 mins 4°C using sterile 250 ml centrifuge tubes (VWR) and concentrated in a 50 ml sterile tubes (VWR ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Cell Biology 2021Quote: ... After solidification of the gelatin block for 6h at 4°C it was embedded in a larger OCT block (Qpath, VWR) and subjected to cryosectioning (10 µm ...