Labshake search
Citations for VWR :
601 - 650 of 814 citations for 6 Chloro 9 2 C methyl β D riboFuranosyl 9H purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 50 mL PBS + 1 M NaCl and 50 mL 2 M Urea (VWR, 97063-798) + 50 mM Ammonium Bicarbonate ...
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Discovery of malathion resistance QTL in Drosophila melanogaster using a bulked phenotyping approachbioRxiv - Genetics 2022Quote: ... and these were plugged with 1/2 of a large cotton ball (VWR, 14224-518) dampened with 1-ml of 10% sucrose solution (Fisher Chemical ...
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bioRxiv - Cell Biology 2023Quote: ... The aliquots were individually heated at different temperatures (Doppio 2×48 well Thermal cycler, VWR). After the heat treatment ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell/DNA mix was then transferred to a pre-chilled 2 mm cuvette (VWR) and pulsed in a GenePulser Xcell (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... and spectinomycin (100 μg/mL) in a 2 mL 96 well deep-well block (VWR) sealed with breathable film (VWR ...
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bioRxiv - Cell Biology 2024Quote: Plated cells were fixed for 10 minutes with 2% paraformaldehyde (PFA; VWR Chemicals, Cat# 28794.364) in phosphate buffer and washed for 3×5 minutes with PBS ...
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bioRxiv - Microbiology 2021Quote: ... The entire amount of plasma (about 180 µL) was filtered using 0.2 µm centrifugal tube from VWR (5 min, 10°C, 10000xg). Next ...
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bioRxiv - Microbiology 2019Quote: ... RNA was then incubated for 10 min at 65 °C and then for 25 min at room temperature with rotation (Tube Rotator, VWR, Radnor, Pennsylvania). Beads were washed with Millipore water ...
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bioRxiv - Cancer Biology 2019Quote: ... in RPMI with 5% FBS and 1% penicillin/streptomycin for 20 minutes in a humidified incubator at 37°C Tissues were washed with PBS and further dissociated in 0.25% Trypsin (VWR, Cat. # VWRL0154-0100) for 30 minutes in the incubator ...
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bioRxiv - Neuroscience 2021Quote: ... Then the tissues were washed in PBS and stored at 4°C with 0.025% (w/v) sodium azide (VWR, Cat#786-299). For cryoprotection ...
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bioRxiv - Microbiology 2021Quote: ... Gryphon cells were cultivated according to manufacturer’s instructions at 5% CO2 37 °C in 10 cm tissue culture-treated dishes (VWR Canada, Mississauga, ON) in DMEM-high glucose (Life Technologies ...
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bioRxiv - Biophysics 2020Quote: VLPs suspended in PBS buffer were mixed at 4°C with KIF5B-spycatcher in (buffer) for 7-8 hours on a nutating mixer (VWR, Radnor, PA) to ensure Spy Tag-Spy Catcher binding ...
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bioRxiv - Immunology 2020Quote: ... for 1h30 at 37°C and the reaction revealed with of 3,3’-5,5’-tetramethylbenzidine peroxidase substrate (100 µL/well - KPL, VWR, Fontenay-sous-Bois, France). Absorbencies were read at 450 nm.
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bioRxiv - Plant Biology 2021Quote: ... Then 200 µL PGM was gently added to the petridish and it was placed in a humid box and incubated at 23°C (incubator, VWR, 89511-416) for 4 or 7 h ...
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bioRxiv - Physiology 2021Quote: ... Tissue samples were embedded in paraffin and sections of 4 μm were coated with 43–45°C Kodak NTB emulsion (VWR, Herlev, Denmark) diluted 1:1 with 43–45°C water ...
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bioRxiv - Neuroscience 2022Quote: ... Then incubated in 10% and 30% sucrose in PBS for 24 hr sequentially at 4 °C and embedded in the OCT compound (cat# 4583; VWR, Randor, PA). Coronal sections were cut at 20 or 50µm on a cryostat (CM3050S ...
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bioRxiv - Microbiology 2019Quote: ... One single colony was picked and incubated for 8 to 10 h at 30°C in either 4.5 ml or 1 ml BHI containing 154mM acetate in reaction tubes or Deepwell-plates (VWR International, Pennsylvania, USA), respectively ...
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bioRxiv - Cell Biology 2021Quote: Aggregation was induced by agitating 5 mg/mL α-synuclein at 37 °C for 7 days 1200 rpm (VWR, Cat#12620-942). Fibril formation was evaluated by electron microscopy (see next section for details) ...
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bioRxiv - Immunology 2020Quote: ... All tissue was digested at 37°C for 30 min with intermittent vigorous shaking before being passed through a 70 μm strainer (VWR, 734-0003) and neutralized with 8% FCS-DMEM buffer ...
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bioRxiv - Microbiology 2022Quote: Y-strain epimastigotes were cultured in LIT/LDNT medium (37) supplemented with 15% 76°C heat-inactivated fetal bovine serum (FBS) (VWR, USDA certified). To obtain amastigotes ...
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bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Physiology 2023Quote: ... we kept the chamber in a 25 −28 °C water bath and then heated the chamber at a rate of 1 °C · min-1 in a water bath (VWR, model: WBE05) containing 2 L of water ...
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bioRxiv - Microbiology 2022Quote: ... albicans isolates were initially cultured by inoculating a single colony in sterile YPD broth at 30°C in an orbital shaker (VWR, model 3500I) at a shaking speed of 200 rpm at a tilted angle of 45° ...
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bioRxiv - Cancer Biology 2023Quote: ... we let the frozen slides reach –20 °C before transferring them directly to 1X PBS/4% paraformaldehyde (PFA) (VWR, cat. no. 10047105). We incubated the sections in PFA for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μl of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, catalog # 97062-376, VWR) (5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... then tumors were measured every 2-3 days using a digital caliper (VWR, Radnor, PA, USA). Tumor volume was calculated with the formula 0.5 × L × W2 where L is the longest diameter and W is the diameter perpendicular to L ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue was homogenized in 2 ml lysis buffer using a Dounce tissue grinder (7ml, VWR). This suspension was supplied with a 1.8M sucrose solution (4 mL) ...
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bioRxiv - Cancer Biology 2020Quote: ... Beads were washed 4X in Kischkel buffer + 2 μg/ml Aprotinin (VWR, Radnor, Pennsylvania, 97062-752) + 1 μg/ml Pepstatin (VWR 97063-246 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Caco-2 cells were cultured in EMEM (ATCC, 30-2003) with 20% FBS (VWR 89510-186), 1% Pen/Strep (Life Technologies 15140122) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Supernatant was supplemented with 2 mM calcium chloride and filtered by a glass microfiber filter (VWR). Samples were then loaded by gravity flow onto 5 ml anti-protein C antibody affinity resin ...
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bioRxiv - Cell Biology 2019Quote: ... while covered with 1.5 ml of imaging media and 2 ml of white mineral oil (VWR). An objective heater (Bioptech ...
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bioRxiv - Microbiology 2020Quote: ... in antibiotic-free LB and 2 mL-deep-96-well plates in checkered pattern (VWR International). Plates were incubated at 37°C with 700 rpm shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Immunology 2020Quote: ... were separately stored in 2 mL pre-filled 2.4mm metal beads tubes (VWR International, Radnor, PA), and quickly snap-frozen in liquid nitrogen (40) ...
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bioRxiv - Cell Biology 2023Quote: ... cells grown in 2- or 4-well chamber slides (model 1 German borosilicate; Lab-Tek; VWR) were fixed with freshly prepared 4% formaldehyde in PBS solution (using 16% Methanol-free Formaldehyde ...
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bioRxiv - Cell Biology 2022Quote: ... glucose was replaced with 2% (w/v) galactose (ITW reagents) or 1.5% (w/v) raffinose (VWR), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... mice expressing hM4D(Gi) were intraperitoneally injected with 2 mg/kg CNO (89160; VWR International, LLC) and returned to their home cages ...
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bioRxiv - Neuroscience 2024Quote: ... pre-chilled 2 ml Pre-Filled Bead Tubes with 2.8 mm Ceramic Beads (VWR, Radnor, PA), and weighed ...
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bioRxiv - Neuroscience 2021Quote: ... 15 min each time, cryopreserved in 30% sucrose (S5-3, Fisher Chemical) in PBS overnight at 4°C and embedded in OCT (VWR International, Wetzlar, Germany). Serial coronal sections (12 μm ...
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bioRxiv - Microbiology 2022Quote: ... Blood was collected by cardiac puncture and then was maintained at 37°C in incomplete RPMI with 25mM HEPES and L-Glutamine (VWR Cat# 45000-412). Infected red blood cells were enriched by an Accudenz discontinuous gradient as previously described [30] ...
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bioRxiv - Microbiology 2020Quote: ... The liquid and solid fractions of a sample were separated by filtration through a combination of three layers of sterile cheesecloth that had been wrapped separately and baked at 175°C and then transported in sealed 15 ml conical Falcon tubes (catalog number: 62406-200, VWR International, Radnor, PA) on dry ice to the laboratory and stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 100 μg/mL of 5-florocystosine (5-FC) and grown at 24°C as listed in Supplemental Table S1 (Stork et al. 2008) (VWR, Radnor, PA, USA). A 10 mg/mL stock solution of 5-FC was prepared in distilled water and filtered sterilized ...
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bioRxiv - Neuroscience 2020Quote: Animals were perfused with 50 ml of 37°C saline and 50 ml of 4% paraformaldehyde (PFA; BDH prolabo, VWR chemicals, Lovaina, Belgium). Brains were extracted and kept in PFA 4% for at least 24h at 4°C and then cut in a fixed material vibratome (VT 1000S ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were sliced into 1mm thick coronal slices at -20°C using a metal brain matrix and .22mm razor blades (Ted Pella, 15045; VWR, 55411-050) and were then placed on dry ice and covered to prevent condensation ...
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bioRxiv - Microbiology 2019Quote: ... The mixture was centrifuged (20 min at 4000 rpm) and the supernatant was evaporated (25°C at 210 rpm) on a rotary evaporator (IKA RV10 digital, VWR, Radnor, PA, USA). The dried crude extract was resuspended in 1 ml of acetonitrile for further analyses ...
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bioRxiv - Microbiology 2021Quote: ... coli 0.1229 and its derivatives were harvested after 16 h shaking at 37°C and passed through 0.2 µm cellulose acetate filters (VWR Life Sciences, Radnor, PA). Assays to quantify Stx amplification were performed as previously described (15) ...
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bioRxiv - Plant Biology 2023Quote: ... Cultures were routinely cultivated in growth chambers (LED-41L2, Percival Scientific, Inc.) at 25°C with continuous shaking on an orbital shaker (VWR OS-500 Shaker) at 120 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were incubated with primary antibodies against the pre- and post-synaptic markers of interest overnight at 4°C in antibody incubation buffer (150 mM NaCl, 50 mM Tris-Base (VWR, Cat# 101174-856), 1% BSA ...
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Sex-specific fear acquisition following early life stress is linked to amygdala glutamate metabolismbioRxiv - Animal Behavior and Cognition 2024Quote: ... The mixture was then incubated at 4°C for 30 minutes with agitation at 1500 rpm using a thermomixer (VWR, Thermal Shake lite).
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bioRxiv - Microbiology 2024Quote: ... and the spores were resuspended in 5 ml of 50% ethanol and incubated at 4°C in a tube rotator (VWR Mini Tube Rotator) running at 15 rpm for 12 h at room temperature to inactivate remaining vegetative cells ...
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bioRxiv - Genetics 2021Quote: ... Amplified emulsion mixture was broken and purified by adding 1 mL of 2-butanol (VWR, AA43315-AK), 50 μL of AMPure XP SPRI (Beckman Coulter ...