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4601 - 4650 of 8568 citations since 2019

• Development of genome-driven, lifestyle-informed primers for identification of the cereal-infecting pathogens Xanthomonas translucens pathovars undulosa and translucens

  Verónica Román-Reyna, et al., bioRxiv - Microbiology 2022

  Quote: ... All PCR products were run in 0.7% agarose gels using 100V for 50 min and visualized under blue light with EZ-Vision Bluelight DNA dye 10,000X (VWR). The thermocycling conditions for all PCRs were ...
• An immunoresponsive three-dimensional urine-tolerant human urothelial (3D-UHU) model to study urinary tract infection

  Nazila V. Jafari, Jennifer L. Rohn, bioRxiv - Microbiology 2022

  Quote: ... Cells were seeded onto polycarbonate transwell inserts with pore size of 0.4 µm (VWR, United Kingdom) at the recommended seeding density (CELLnTEC ...
• Digital color-coded molecular barcoding reveals dysregulation of common FUS and FMRP targets in soma and neurites of ALS mutant motoneurons

  Maria Giovanna Garone, et al., bioRxiv - Molecular Biology 2022

  Quote: ... The membrane was lysed with TRK lysis buffer of the Micro Elute Total RNA Kit (VWR International PBI) and left rotating on a wheel for 20 min at room temperature ...
• APE1 recruits ATRIP to ssDNA in an RPA-independent manner to promote the ATR DNA damage response

  Yunfeng Lin, et al., bioRxiv - Molecular Biology 2022

  Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
• A living conductive marine biofilm engineered to sense and respond to small molecules

  Lina J. Bird, et al., bioRxiv - Microbiology 2022

  Quote: ... Rubber stoppers (VWR) were used for all the reactor openings ...
• Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

  Julia Kolb, et al., bioRxiv - Neuroscience 2022

  Quote: ... 3% Acetonitrile (VWR Cat#83640.320), 1 μg of LysC (Wako Cat#129-02541) ...
• Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

  Julia Kolb, et al., bioRxiv - Neuroscience 2022

  Quote: ... the digestion mixture was diluted 1:3 with 10% Acetonitrile in MS grade water (VWR Cat#83645.320), followed by sonication in the Bioruptor Plus sonication system and incubation with 1 μg of LysC and 2 μg of Trypsin (Promega Cat#V511A ...
• Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

  Julia Kolb, et al., bioRxiv - Neuroscience 2022

  Quote: Peptides were solubilized in 6 μL buffer A (100% MS-LC grade water and 0.1% Formic acid (VWR Cat#84865.180), and a total volume of 3 μL were loaded onto a 30 cm-long column (75 μm inner diameter (Polymicro Cat#TSP075375) ...
• Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

  Julia Kolb, et al., bioRxiv - Neuroscience 2022

  Quote: ... 500 mM NaCl (VWR Cat#27810.295), 1% NP40 (Fluka Cat#74385) ...
• Excitatory amino acid transporter 1 supports adult hippocampal neural stem cell self-renewal

  Joshua D. Rieskamp, et al., bioRxiv - Neuroscience 2022

  Quote: ... and anti-rabbit IR800 (VWR 103011-494) both diluted 1:5000 in blocking buffer ...
• Excitatory amino acid transporter 1 supports adult hippocampal neural stem cell self-renewal

  Joshua D. Rieskamp, et al., bioRxiv - Neuroscience 2022

  Quote: ... Membranes were then rinsed 3x in TBS and incubated for 1 hour in anti-mouse IR680 (VWR 102971-020) and anti-rabbit IR800 (VWR 103011-494 ...
• Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation

  Taru Hilander, et al., bioRxiv - Molecular Biology 2022

  Quote: DNA was extracted from HEK-293 cells using DirectPCR® DNA Lysis Reagent (VWR, 732-3255) according to the manufacturer’s instructions ...
• Nuclear SUN1 Stabilizes Endothelial Cell Junctions via Microtubules to Regulate Blood Vessel Formation

  Danielle B Buglak, et al., bioRxiv - Cell Biology 2022

  Quote: ... for 15 min at 37°C or 10µM Y-27632 (10187-694, VWR) for 30 min at 37°C then immediately fixed in 4% PFA ...
• PRR14 organizes H3K9me3-modifed heterochromatin at the nuclear lamina

  Anna A. Kiseleva, et al., bioRxiv - Cell Biology 2022

  Quote: ... then scraped off the plates in 500 μL ice-cold NP-40 lysis buffer (VWR Life Science, cat# J619-500ML) containing protease and phosphatase inhibitors (Roche ...
• Distinct cytosolic complexes containing the type III secretion system ATPase resolved by 3D single-molecule tracking in live Yersinia enterocolitica

  Joshua R Prindle, et al., bioRxiv - Microbiology 2022

  Quote: ... Immersion oil was placed between the objective lens (UPLSAPO 100 1.4 NA) and the glass cover slip (VWR, Radnor ...
• HERBS: Histological E-data Registration in rodent Brain Spaces

  Jingyi Guo Fuglstad, et al., bioRxiv - Neuroscience 2022

  Quote: The brain was transferred to 2% dimethyl sulfoxide (DMSO; VWR, USA) solution for cryoprotection for 1-2 days ...
• FOXA1/2 depletion drives global reprogramming of differentiation state and metabolism in a human liver cell line and inhibits differentiation of human stem cell-derived hepatic progenitor cells

  Iyan Warren, et al., bioRxiv - Bioengineering 2022

  Quote: ... Bovine Serum Albumin (Cat. #: 10791-790) was purchased from VWR International (Radnor ...
• Rapid and sensitive single cell RNA sequencing with SHERRY2

  Lin Di, et al., bioRxiv - Genomics 2022

  Quote: ... 16% PEG8000 (VWR Life Science, 97061), 0.5mM ATP (NEB ...
• Chlamydia trachomatis induces the transcriptional activity of host YAP in a Hippo-independent fashion

  Liam T. Caven, et al., bioRxiv - Cell Biology 2022

  Quote: End1s or HCECs seeded on glass cover slips (VWR) coated with fibronectin (Corning 354008 ...
• Maraviroc inhibits SARS-CoV-2 multiplication and s-protein mediated cell fusion in cell culture

  Kenneth H. Risner, et al., bioRxiv - Microbiology 2022

  Quote: ... African green monkey kidney cells (Vero) were maintained in DMEM supplemented with 5% heat-inactivated fetal bovine essence (FBE) (VWR Life Sciences 10803-034), 1% Penicillin/Streptomycin (Gibco ...
• Maraviroc inhibits SARS-CoV-2 multiplication and s-protein mediated cell fusion in cell culture

  Kenneth H. Risner, et al., bioRxiv - Microbiology 2022

  Quote: ... and 1% L-Glutamine (VWR Life Sciences, VWRL0131-0100) at 37 °C and 5% CO2 ...
• Distinct mechanisms underlie H₂O₂ sensing in C. elegans head and tail

  Sophie Quintin, et al., bioRxiv - Cell Biology 2022

  Quote: ... we used a soft bake f 7min at 95°C on hot plates (VWR) followed by a UV 365nm exposure at 160 mJ/cm2 with a mask aligner (UV-KUB3 Kloé® ...
• Preliminary Characterization of Phage-like Particles from the Male-Killing Mollicute Spiroplasma poulsonii (an Endosymbiont of Drosophila)

  Paulino Ramirez, et al., bioRxiv - Microbiology 2022

  Quote: ... 1% (w/v) gelatin (VWR Cat. No. 97062-618) and 1μg/ml RNase A (Thermofisher Cat ...
• Desmin intermediate filaments and tubulin detyrosination stabilize growing microtubules in the cardiomyocyte

  Alexander K. Salomon, et al., bioRxiv - Cell Biology 2022

  Quote: ... mouse monoclonal (VWR GenScript A01622-40); western blot ...
• Proteolysis-free cell migration through crowded environments via mechanical worrying

  Meghan K. Driscoll, et al., bioRxiv - Cell Biology 2022

  Quote: ... WGA was purchased from VWR (101098-084). 70 kDa Fluorescein isothiocyanate–dextran was purchased from Sigma (46945).
• Determination of oligomeric states of proteins via dual-color colocalization with single molecule localization microscopy

  Hua Leonhard Tan, et al., bioRxiv - Cell Biology 2022

  Quote: ... Piranha solution reacts violently with organic matter and must be handled and disposed of according to institutional guidelines! Coverslips (VWR) were placed in a glass container and freshly prepared piranha solution was added ...
• Gene amplification acts as a molecular foothold to facilitate cross-species adaptation and evasion of multiple antiviral pathways

  Shefali Banerjee, et al., bioRxiv - Evolutionary Biology 2022

  Quote: ... Human foreskin fibroblasts (HFF, kindly provided by Denise Galloway) were maintained in Minimum Essential Media-α (MEM-α; VWR) supplemented with 20% FBS (GE Healthcare ...
• The catheterized bladder environment promotes Efg1- and Als1-dependent Candida albicans infection

  Alyssa Ann La Bella, et al., bioRxiv - Microbiology 2022

  Quote: Biofilm formation assays were performed in 96 well flat-bottomed plates (VWR 10861-562) coated with 100uL of BSA or Fg (150 μg/mL ...
• The catheterized bladder environment promotes Efg1- and Als1-dependent Candida albicans infection

  Alyssa Ann La Bella, et al., bioRxiv - Microbiology 2022

  Quote: ... 20 g/L Dextrose (VWR BDH9230-500G)) broth ...
• The catheterized bladder environment promotes Efg1- and Als1-dependent Candida albicans infection

  Alyssa Ann La Bella, et al., bioRxiv - Microbiology 2022

  Quote: ... albicans were cultured at 37 °C with aeration in 5 mL of YPD (10g/L Yeast Extract (VWR J850-500G), 20g/L Peptone (VWR J636-500G) ...
• The catheterized bladder environment promotes Efg1- and Als1-dependent Candida albicans infection

  Alyssa Ann La Bella, et al., bioRxiv - Microbiology 2022

  Quote: ... Biofilms were incubated with anti-Candida antibodies diluted into dilution buffer (PBS with 0.05% Tween (VWR M147-1L), 0.1% BSA ...
• The catheterized bladder environment promotes Efg1- and Als1-dependent Candida albicans infection

  Alyssa Ann La Bella, et al., bioRxiv - Microbiology 2022

  Quote: ... 20g/L Peptone (VWR J636-500G), 20 g/L Dextrose (VWR BDH9230-500G) ...
• SERF deletion modifies amyloid aggregation in a mouse model of Alzheimer’s disease

  E. Stroo, et al., bioRxiv - Molecular Biology 2022

  Quote: ... for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent, VWR).
• SERF deletion modifies amyloid aggregation in a mouse model of Alzheimer’s disease

  E. Stroo, et al., bioRxiv - Molecular Biology 2022

  Quote: ... DNA was purified using prepGEM® Tissue kit according to a protocol adapted from the manufacturer (ZYGEPTI0500, ZyGEM, VWR International BV) and subjected to PCR using the primers listed in Supplemental Table S5 ...
• An Optimized Buffer for Repeatable Multicolor STORM

  Vaky Abdelsayed, et al., bioRxiv - Biophysics 2022

  Quote: ... Cells were plated at low confluency on ethanol-cleaned 25 mm #1.5 thickness round coverglass (VWR) for imaging ...
• Fendrr synergizes with Wnt signalling to regulate fibrosis related genes during lung development via its RNA:dsDNA Triplex Element

  Tamer Ali, et al., bioRxiv - Developmental Biology 2022

  Quote: ... For direct RNA isolation the lung tissue was transferred into Precellys beads CK14 tubes (VWR, 10144-554) containing 1ml 900 μl Qiazol (Qiagen ...
• Recruitment of Polo-like kinase couples synapsis to meiotic progression via inactivation of CHK-2

  Liangyu Zhang, et al., bioRxiv - Cell Biology 2022

  Quote: ... beads were spun in a 10K MWCO filter (VWR, Radnor, PA) at room temperature for 10 minutes at 10,000 x g and then washed with 50 mM ammonium bicarbonate ...
• miR167-ARF8, an auxin-responsive module involved in the formation of root-knot nematode-induced galls in tomato

  Yara Noureddine, et al., bioRxiv - Plant Biology 2022

  Quote: ... Sections were cut and mounted in DPX (VWR International Ltd, Poole, UK), and observed under a Zeiss Axioplan 2 microscope (Zeiss ...
• A genetically-encoded three-colour stress biosensor reveals multimodal response at single cell level and spatiotemporal dynamics of biofilms

  Ahmed E. Zoheir, et al., bioRxiv - Microbiology 2022

  Quote: ... respectively) into 250 μL of RNAPure™ peqGOLD (VWR International GmbH), then immediately vortexed and kept on ice until extraction.
• Comprehensive multi-omics characterization of gut microbiome extracellular vesicles reveals a connection to gut-brain axis signaling

  Salma Sultan, et al., bioRxiv - Microbiology 2022

  Quote: ... All cells were maintained at 37 °C in a 5% CO2 humidified incubator (VWR, Mississauga, Ontario). For subculturing ...
• B/T cell crosstalk and aberrant inflammatory IgG exacerbate autoimmune intestinal inflammation

  Iana Gadjalova, et al., bioRxiv - Immunology 2022

  Quote: ... and fixed with 4% paraformaldehyde (PFA, VWR international). Cells were blocked with anti-mouse CD16/32 (93 ...
• B/T cell crosstalk and aberrant inflammatory IgG exacerbate autoimmune intestinal inflammation

  Iana Gadjalova, et al., bioRxiv - Immunology 2022

  Quote: ... murine colon rolls were fixed in 4%(w/v) PFA (VWR International), equilibrated in 30%(w/v ...
• Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid model

  Yu-Han Hung, et al., bioRxiv - Genomics 2022

  Quote: ... 100 ng yeast tRNA (VWR, 80054–306), 0.8 units/μL SUPERase In RNase Inhibitor ...
• Spatial host-microbiome sequencing

  Britta Lötstedt, et al., bioRxiv - Genomics 2022

  Quote: ... The slide was removed from the holder and air dried before mounted with 85% glycerol and covered with a cover slip (VWR, USA) prior to imaging ...
• The Toll pathway mediates Drosophila resilience to Aspergillus mycotoxins through specific Bomanins

  Rui Xu, et al., bioRxiv - Immunology 2022

  Quote: ... 90 g nipagin (VWR Chemicals) were diluted into 350 ml ethanol (Sigma-Aldrich) ...
• A broad, exposome-type evaluation of xenobiotic phase II biotransformation in human biofluids by LC-MS/MS

  Yasmin Fareed, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... LC-MS grade water was obtained from VWR chemicals (Vienna ...
• Receptor levels determine binding affinity of WNT-3A to Frizzled 7 in a colorectal cancer model

  Lukas Grätz, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... a white adhesive tape (VWR) was attached to the plate bottom ...
• Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines

  Avital Licht-Murava, et al., bioRxiv - Neuroscience 2022

  Quote: ... 10% heat-inactivated FBS (VWR #89510-188), 1X GlutaMAX (ThermoFisher #35050061) ...
• Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines

  Avital Licht-Murava, et al., bioRxiv - Neuroscience 2022

  Quote: ... before sealing #1.5 coverglass (VWR #89239-734) with Vectashield antifade media containing DAPI (VWR #101098-050) ...
• The 5:2 diet does not increase adult hippocampal neurogenesis or enhance spatial memory in mice

  Luke D. Roberts, et al., bioRxiv - Neuroscience 2022

  Quote: ... Sections were mounted onto superfrost+ slides (VWR, France) and allowed to dry overnight before being de-hydrated and de-lipified in increasing concentrations of ethanol ...