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Citations for VWR :
401 - 419 of 419 citations for n4 Benzoyl 5 methyldeoxycytidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Concentration and quality of total RNA isolations using a phenol-chloroform extraction with Isol-RNA lysis reagent (5’Prime, Hamburg, Germany) from endothelial cells were verified by a NanoDrop™ 1000 (Peqlab VWR, Radnor, PA) and an Agilent 2100 bioanalyzer (Agilent ...
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bioRxiv - Biophysics 2020Quote: ... The sheath and sample fluid syringes (BD Luer-Lok™ 5-ml syringe #613-2043P and BD Luer-Lok™ 1-ml syringe #613-4971; VWR, Germany) were connected to the fabricated chip via FEP Tubing (1/16” OD ...
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bioRxiv - Cancer Biology 2020Quote: ... the exons of interest in PD-L1 gene were amplified by using specific primers (Table 5) with a PCR thermal cycler (VWR Thermocycler, Leicestershire, England). PCR procedures were performed with a standard reaction mixture (4 μl/sample of Phusion GC Buffer (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2020Quote: Leaf tissue was collected 7 days post infection, transferred to an extraction bag (Bioreba, Switzerland) and mixed with 5 ml of phosphate buffered saline (VWR, West Chester, PA), hand homogenized using a hand-held tissue homogenizer (Agdia ...
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bioRxiv - Molecular Biology 2021Quote: ... or 5% (w/v) non-fat dry milk in TRIS-buffered saline (TBS) containing 0.1% Tween-20 (TBS-T; VWR, Radnor, PA, USA, # 28829.183) for one hour at room temperature and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 12 µg of plasmid DNA (ratio 1:10 for guide-RNA and dCas9-construct containing plasmids) was diluted into 5 µl of sterile 10 % glucose and added to diluted In vivo-jetPEI® reagent (#101000040 VWR, Polyplus-transfection). The solution was mixed by pipetting and incubated at RT for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... and the spores were resuspended in 5 ml of 50% ethanol and incubated at 4°C in a tube rotator (VWR Mini Tube Rotator) running at 15 rpm for 12 h at room temperature to inactivate remaining vegetative cells ...
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bioRxiv - Neuroscience 2023Quote: ... to a 50-mL Falcon tube on ice and homogenized at a speed of ∼1000 rpm for 5 sec to brake nuclei clumps with a handheld homogenizer (VWR® 200 Homogenizer). After homogenization ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were resuspended in 5 mL of methanol/dichlormethane/ethylacetate 10:2:3 (dichlormethane: SupraSolv, VWR Chemicals, Dresden, Germany; ethylacetate, HiPerSolv Chromanorm, VWR Chemicals, Dresden, Germany). The suspensions were sonicated (5 min ...
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bioRxiv - Bioengineering 2024Quote: ... Cellular debris was then cleared by centrifugation at 1,000 g for 5 minutes at 4°C and then further filtered through a 0.45 μm cellulose acetate filter (VWR cat. no. 28145-481). The cleared media was then concentrated with 100 kDa Amicon cutoff filter (Millipore Sigma cat ...
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bioRxiv - Biochemistry 2024Quote: ... The blots were incubated in 5% nonfat dry milk prepared in 1X Tris-buffered saline with Tween 20 (TBS-T, VWR International, #J63682.K2) for 30 min at room temperature to block nonspecific binding sites ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with 90% trypan blue for 5 min and trypan blue exclusion was measured using SD100 counting chambers (VWR; Cat. No. MSPP-CHT4SD100) and a cell counter (Nexcelom Cellometer Auto T4) ...
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bioRxiv - Microbiology 2022Quote: ... African green monkey kidney cells (Vero) were maintained in DMEM supplemented with 5% heat-inactivated fetal bovine essence (FBE) (VWR Life Sciences 10803-034), 1% Penicillin/Streptomycin (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Microscope slides with serially cut nerves were rinsed in 1x PBS for 5 min and air dried by incubation at 60°C for 45 min in an oven (VWR Scientific, Model 1525 incubator). Next ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed twice (5 min each) in RNAase and DNAase free PBS before mounting onto Superfrost Plus coated slides (VWR, catalog no: 48311-703) in RNase- and DNase-free PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were first incubated for 1 h in blocking solution (0.01 M PBS with 0.3% Triton X-100, Sigma, T9284; 5% normal donkey serum, NDS, VWR, 80054-446 or Calbiochem, 566460-5ML) at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Tissues were dehydrated using an ascending methanol series and bleached overnight at 4 °C in a solution containing 5% H2O2 (VWR Chemicals, Radnor, PE, USA) in methanol ...
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bioRxiv - Genetics 2024Quote: ... 5% glycerol) supplemented with protease inhibitors (Leupeptin, Sigma-Aldrich, 11034626001; Aprotinin, Santa Cruz Biotechnology, sc3595; Pepstatin A, VWR, 97064-248; PMSF, Sigma-Aldrich, 7110), phosphatase inhibitors (Sodium Orthovanadate ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.5 (Applichem) and transferred to a 30 kDa AcroPrep Omega filter membrane plate (PALL, New York, US, purchased via VWR, Hannover, Germany, REF 8035/518-0028) in a blocked randomized order ...