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Citations for VWR :
351 - 400 of 1188 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR). Peak fractions were collected ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to nitrocellulose overnight in 20% methanol in Tris/Glycine transfer buffer (VWR, #97061-382). Total protein was visualized using the LI-COR REVERT™ Total Protein Stain (Licor ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Each bone was then cut in half and transferred to a 1.5 ml microcentrifuge tube containing a G-Tube microcentrifuge tube (VWR, Radnor, PA, USA) that had been punctured at the bottom with a 15 gauge needle ...
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bioRxiv - Bioengineering 2022Quote: ... centrifuged for 3 minutes at 300 g and resuspended in E8 flex medium containing 10 μM Y27632 ROCK inhibitor (VWR, #688000-5). Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning ...
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bioRxiv - Cancer Biology 2020Quote: 1.2 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cancer Biology 2020Quote: 3 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-742). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Microbiology 2023Quote: ... were transfected into single wells (3.5 cm) of the Corning Costar 6-well cell culture plate containing HEK293T/17 cells using the JetPrime Transfection reagent (VWR, Randor, PA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 1% agar [Teknova, A7777] containing 40 µg/mL Congo red dye [VWR, AAAB24310-14] and 20 µg/mL Coomassie blue dye [VWR, EM-3300]) in a 10 cm X 10 cm X 1.5 cm plates ...
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bioRxiv - Microbiology 2023Quote: ... The AB food consisted of standard media containing 200 mg/ mL of ampicillin and kanamycin (VWR Life Science; both dissolved in water), 50 mg/ mL of tetracycline (EMD Millipore Corp. ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were put into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). The columns were equilibrated in high salt buffer (20mM Tris-HCL ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then loaded into 5’polystyrene chromatography columns with coarse filters (Evergreen, 208-3383-060) containing DEAE Sephadex A-25 resin (VWR, 95055-928). Once the sample completely flowed through the column ...
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bioRxiv - Genetics 2023Quote: ... 500μl of concentrated bacterial suspension or 5% (w/v) sucrose solution (mock treatment) was added to empty fly vials containing a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... 400,000 cells were seeded on ethanol-sterilised coverslip in 6 well 3.5 cm diameter plate containing 22 × 22 mm glass coverslips (VWR. Cat# 631-0125). 2 μg of DNA and 6 μg of PEI were diluted in 0.25 ml of Opti-MEM™ Reduced serum medium (Gibco™) ...
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bioRxiv - Biochemistry 2023Quote: ... Waltham, MA) containing 0.1% anticlumping agent (Thermo Fischer Scientific, Waltham, MA) in a 125 mL unbaffled shake flask (VWR, Radnor, PA, USA) with a 40 mL working volume ...
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bioRxiv - Cancer Biology 2024Quote: FFPE tissue blocks containing human primary tumors were sectioned at 4 mm onto Superfrost Plus microscope slides (VWR International, catalog 48311-703). Automated immunohistochemistry was performed at the Oncology Tissue and Imaging Services Core of the Johns Hopkins University School of Medicine using a Ventana Discovery Ultra automated slide staining system (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... cell line developed and characterized by our laboratory was used in this study.(31) Cells were maintained in defined serum-free keratinocyte growth medium (KGM) containing growth factor supplements (KGM, VWR, Randor, PA) and a 10% solution consisting of penicillin ...
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bioRxiv - Biophysics 2021Quote: ... Cell culture media growth factors and antibodies were purchased from VWR International PBI (Milan ...
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bioRxiv - Cell Biology 2023Quote: ... IRDye 800CW Goat-anti-Rabbit antibody (VWR, Cat No:102673-330) and IRDye 680RD Goat-anti-Mouse antibody (VWR ...
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bioRxiv - Microbiology 2024Quote: ... Rpoβ (anti-RNA polymerase beta mouse monoclonal antibody [clone: 8RB13]; VWR) was diluted 1:20,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Rabbit anti-S100 1:300 (VWR/ProteinTech 15146-1-AP), Chick anti-Neurofilament 200 kDa 1:500 (Aves NFH) ...
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bioRxiv - Neuroscience 2020Quote: ... A maximum of 7.5% of the body weight was obtained in BD vacutainer tubes containing EDTA as anticoagulant (VWR, Ville Mont-Royal, Quebec). Adolescent mice were closely monitored for bleeding ...
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bioRxiv - Microbiology 2022Quote: ... Supplemented M9 containing 0.85 mg/mL pluronic F127 without antibiotics was flowed via Tygon Microbore silicon tubing (VWR ND 100-80/0,508*1,524) that linked the device to a syringe pump (ALADDIN-220 ...
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bioRxiv - Molecular Biology 2021Quote: ... or 5% (w/v) non-fat dry milk in TRIS-buffered saline (TBS) containing 0.1% Tween-20 (TBS-T; VWR, Radnor, PA, USA, # 28829.183) for one hour at room temperature and incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: Antibiotic tolerance assays using co-cultures were performed using a membrane-separated 12-well tissue plate cultures containing 0.1 μm pore PET membranes (VWR, Cat. No. 10769–226), following previously protocols (Meirelles et al. ...
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bioRxiv - Microbiology 2024Quote: ... 450 µL of the upper phase was transferred to a new tube containing 500 µL of cold isopropanol (VWR, Fontenay-sous-Bois, France) and 45 µL of 3M sodium acetate (Carl Roth ...
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bioRxiv - Cell Biology 2020Quote: ... the medium was removed and filtered through a 0.45-µm low-protein binding membrane (VWR International, Radnor, PA). McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... all proteins were diluted in filtered (sterile syringe filters 0.45 μm cellulose acetate membrane, VWR International, Radnor, USA) Min buffer in the presence of either 0.5 mM ADP or ATP ...
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bioRxiv - Genetics 2020Quote: Quadriceps from P10 pups were dissected and total proteins extracted using radioimmunoprecipitation assay (RIPA) buffer (VWR, Radnor, PA) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific) ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... The sheared DNA was then pre-cleared using magnetic beads (Sera-Mag Protein A/G SpeedBeads, VWR 17152104010150) for 1 hour to remove non-specific binding ...
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bioRxiv - Microbiology 2024Quote: ... Enzyme solution was prepared in distilled water and the protein concentration was determined using Bradford reagent (VWR, USA) with bovine serum albumin as the protein standard ...
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bioRxiv - Cell Biology 2024Quote: 25 µg of proteins were separated in SDS-PAGE gels before being transferred to nitrocellulose membranes (VWR 10600002). After ponceau staining ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% DMSO (VWR) and 0.2 mM 1-phenyl-2-thiourea (Acros Organics ...
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bioRxiv - Neuroscience 2024Quote: ... 1% CHAPS (VWR) and 50mM HEPES (pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... + 1% BSA (VWR)) to a density of 1 million cells/ml following trypsinization (Caco2 and DLD1 ...
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bioRxiv - Cell Biology 2023Quote: ... and IRDye 680RD Goat-anti-Mouse antibody (VWR, Cat No:102673-408) at 1:20,000 dilutions ...
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bioRxiv - Biochemistry 2024Quote: ... 10 ul of phycoerythrin (PE)-conjugated anti-Pgp antibodies (VWR, 348606-BL) or isotype controls (VWR ...
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bioRxiv - Microbiology 2021Quote: ... the virus suspension was mixed 1:1 with peqGOLD TriFast (VWR). Following ...
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bioRxiv - Molecular Biology 2023Quote: ... then neutralized with 1 μL 5 M HCl (VWR BDH7419-1). The remainder of the library preparation was performed according to the xGen protocol except that post-ligation ...
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bioRxiv - Cancer Biology 2020Quote: Primary OSE cultures and the OSN2 cell line (Flesken-Nikitin et al. 2003) were maintained in culture in media containing DMEM (VWR; Cat#: 10-017-CM), Hams F12 (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... co-culture experiments were performed using membrane-separated 12-well tissue plate cultures containing 0.1 µm pore PET membranes (VWR® Cat. No. 10769-226). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Tissues were dehydrated using an ascending methanol series and bleached overnight at 4 °C in a solution containing 5% H2O2 (VWR Chemicals, Radnor, PE, USA) in methanol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and then 1 hour in 1:1 ratio of 30% sucrose/0.1M phosphate buffer:OCT (VWR, Cat No. 25608-930). Tissue was embedded inside a mold made by the severed end of a 1.5mL microcentrifuge tube attached to a glass slide with nail polish and stored at −80°C until used ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were prepared according to the manufacturer’s instructions and proteins were separated in 10 % Next Gel SDS-PAGE (VWR). Carbonylated proteins were detected with Immobilon Western Chemiluminescent HRP Substrate (MilliporeSigma).
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bioRxiv - Bioengineering 2022Quote: ... 50/15 mL Falcon tubes and 50/5/2 mL DNA/Protein loBind Eppendorf tubes were purchased from VWR. D02-E100-05-N and C02-E100-05-N tangential flow filtration filters were purchased from Repligen ...
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bioRxiv - Immunology 2022Quote: ... The protein fraction was isolated by combining four times the volume of ice cold acetone (VWR Chemicals, PA, USA) to one volume of reconstituted PBMCsec ...
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bioRxiv - Systems Biology 2020Quote: ... Protein and crosslink digestion was carried out by adding 50 μL freshly prepared 20 mg/mL Proteinase K (VWR, freeze-dried powder suspended in 10 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... cultured in 150mm dish (Alkali scientific TDN0150) for protein experiment and in 6-well culture plate (VWR, 100062-892) for total RNA experiment and transfected at around 50-60% confluency ...