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Citations for VWR :
351 - 400 of 1187 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Emerging cooperativity between Oct4 and Sox2 governs the pluripotency network in mouse early embryosbioRxiv - Developmental Biology 2024Quote: ... The 3’ end enriched libraries were constructed using KAPA HyperPlus Library Preparation Kit (VWR International, KK8513) with 6 cycles of PCR ...
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bioRxiv - Bioengineering 2024Quote: ... 200 mg of paKG polymers were dissolved in 3 mL of dichloromethane (DCM) (VWR, Radnor, PA). 10 mg of PFK15 (Selleckchem ...
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bioRxiv - Immunology 2024Quote: ... with media changes every 2-3 days before expansion to a T-75 flask (CellStar, VWR).
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bioRxiv - Physiology 2021Quote: ... incubated at 37°C for 120 min and inactivated at 85°C for 5 min in a thermal cycler (VWR UNO 96). cDNA was then stored at −20°C until further use ...
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bioRxiv - Microbiology 2022Quote: ... — were incubated overnight at 30°C in 5 mL of Marine Broth 2216 (VWR) in a shaking incubator at 220 rpm ...
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bioRxiv - Bioengineering 2023Quote: ... Mouse uterus cross-section tissue was fixed for 30 minutes in 4% formaldehyde (VWR) before blocking for 30 minutes in 10% Normal Goat Serum (50062Z ...
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bioRxiv - Microbiology 2023Quote: Samples were analyzed using standard SDS-PAGE (4-16% bis acrylamide, Precast gels, VWR) and immunoblotting techniques ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% dextrose [VWR #90000– 904] ...
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bioRxiv - Cell Biology 2021Quote: ... P1 virus was propagated to passage 2 (P2) by infecting 50 ml of SF9 (VWR, #EM71104-3) culture and harvesting after 5–7 days as described above ...
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bioRxiv - Plant Biology 2021Quote: ... 3.5 mL of 18.2 MΩ deionised water (Elga PURELAB flex 3 system, VWR International, Edmonton, AB, Canada) was then added to effect a separation into a polar methanol/water layer and a chloroform layer ...
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bioRxiv - Microbiology 2021Quote: HEK 293T cells (3×105 cells) were plated on the sterilized round 15mm #1 glass coverslips (VWR) in 12-well plates and seeded for 24hours prior to transfection ...
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bioRxiv - Bioengineering 2020Quote: ... Then we denatured the phage protein coat by adding 7.5 ml of 3 M KOAc (VWR international), pH 5.5 ...
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bioRxiv - Bioengineering 2021Quote: ... On day 3 the cells were collected using Trypsin ethylenediamine tetraacetic acid (EDTA) (VWR, Chicago, IL, USA). Cells were mixed with either GelMA (10% w/v final ...
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bioRxiv - Cancer Biology 2020Quote: ... Successively the endogenous peroxidases and non-specific binding sites were blocked using 3% H2O2 (VWR chemicals, 23615.248) and Protein-Block solution (DAKO Agilent technologies ...
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bioRxiv - Cell Biology 2022Quote: ... The micropipette was then sandwiched between a 1-mm glass cover slide (3”x1”x 1mm; VWR) and a #1.5 glass cover slip (24×60 mm ...
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bioRxiv - Neuroscience 2022Quote: ... the digestion mixture was diluted 1:3 with 10% Acetonitrile in MS grade water (VWR Cat#83645.320), followed by sonication in the Bioruptor Plus sonication system and incubation with 1 μg of LysC and 2 μg of Trypsin (Promega Cat#V511A ...
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bioRxiv - Microbiology 2024Quote: ... Moore swabs were constructed by folding 3 by 24-inch strips of cheese cloth (VWR, Radnor, PA) 8 times to form an 8-ply square pad ...
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bioRxiv - Cancer Biology 2024Quote: PDAC-3 spheroids were grown in VWR®96-wells F-bottom plates (VWR, Cat No. 7342327) coated with 1.5% w/v agarose ...
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bioRxiv - Biochemistry 2024Quote: ... and inoculated on a Nutrient Agar plate containing 100 μg/mL ampicillin (VWR; Cas: 69-52-3). After culturing overnight at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... sections were again washed in PBS for 6 × 5 mins before mounting onto polylysine-coated slides (Polysine, VWR). Mounted slices were left to dehydrate overnight before applying coverslips using either Aqua-Poly/Mount (Polysciences Inc. ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cultures were maintained in an 37°C incubator with 5% CO2 and relative humidity (VWR).
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bioRxiv - Cell Biology 2021Quote: ... the gels were washed once with PBS and then fixed with 4% paraformaldehyde (VWR, 43368) for 10 min ...
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bioRxiv - Physiology 2021Quote: Testes from all sampled fish were dissected and fixated in 4 % glutaraldehyde (VWR, Radnor, USA) overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... The inflated left lobe was excised and fixed in PBS/4% (v/v) formaldehyde (VWR) for 2 hours at 4°C.
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bioRxiv - Neuroscience 2020Quote: ... We used borosilicate capillary glass needles (thick-walled, with internal filaments, Cat 1B150F-4, VWR), pulled with a Flaming/Brown pipette puller (Model P-97 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... All tissues were fixed using neutral buffered formaldehyde solution (NBF, 4% formaldehyde, VWR Chemicals 9713.9025) of at least ten times the volume of the dissected specimens for 14-18 h at 4°C with gentle agitation in the dark.
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bioRxiv - Bioengineering 2023Quote: ... The tissue constructs were immediately fixed following excision in 4% (v/v) paraformaldehyde (PFA; VWR) for 72 hours at 4°C and then washed with PBS for 3 ...
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bioRxiv - Bioengineering 2023Quote: ... The permeabilized cells were then blocked with 4% Bovine Serum Albumin (BSA) (VWR, PA, USA) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown on 4-well chamber slides (model 1 German borosilicate; Lab-Tek; VWR) to 30-50% confluency ...
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bioRxiv - Bioengineering 2023Quote: ... Cell monolayers were then rinsed three times with PBS and fixed with 4% paraformaldehyde (VWR) for 15 min ...
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bioRxiv - Immunology 2024Quote: Primary human fetal tissue was fixed for one week in 4% formaldehyde (VWR #87001-890) in PBS at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... a Gram’s Iodine test was carried out by adding 4 mL of Gram’s solution (VWR) to each plate to cover the colonies and set at room temperature for one minute.
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bioRxiv - Developmental Biology 2024Quote: ... they were washed once with PBS and then fixed in 4% Paraformaldehyde (VWR # CAAAJ61899-AP) for 8 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then passaged and selected using 4 μg/mL G418 (Cat#97064-358, VWR), until the control cell population reached 100% lethality ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 100 μg/mL of 5-florocystosine (5-FC) and grown at 24°C as listed in Supplemental Table S1 (Stork et al. 2008) (VWR, Radnor, PA, USA). A 10 mg/mL stock solution of 5-FC was prepared in distilled water and filtered sterilized ...
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bioRxiv - Cell Biology 2020Quote: ... 3 washes of 15 minutes with 5X SSCT (1:10,000 DAPI TCA2412-5MG, VWR in the second wash), and storage/imaging in 5X SSCT ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor growth was monitored weekly and tumor volume was measured along 3 axes with calipers (VWR 62379-531). Tumors and lungs were harvested at time of tumor burden (total tumor volume of 2cm3 ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... Equal amounts of plasmid (2 μg total) were co-transfected with 3 μL 25kDa PEI (VWR, Radnor, PA) into 293T cells into a 24-well plate.
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bioRxiv - Immunology 2022Quote: 3 µg/ml of spike antigens were coated onto the 384-well ELISA plates (VWR, Cat # 82051-300) overnight at 4 degree ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated overnight at room temperature on a shaker in presence of 3 μg LysC Endopeptidase (VWR). Next ...
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bioRxiv - Genomics 2020Quote: ... were co-transfected with the respective lentiviral expression vector (∼1:1:3 molar ratio) using Trans-IT (VWR) and OptiMEM (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 μm-thick sections were obtained and counterstained with Liquid-Stable Nuclear Fast Red (VWR Life Science AMRESCO).
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bioRxiv - Cancer Biology 2022Quote: ... cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, VWR, PA, USA) assay ...
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bioRxiv - Microbiology 2022Quote: ... The filtrate was obtained by individually centrifuging 250 µl of each dilution at 5000 × g for 3 minutes through a 0.2 µm spin filter (Centrifugal Filter, Modified Nylon 0.2 μm, VWR). Both sample types ...
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bioRxiv - Genetics 2023Quote: The muscarinic receptor antagonist atropine ((RS)-(8-Methyl-8-azabicyclo[3.2.1]oct-3-yl, VWR Cat. TCA0754-005G) was dissolved in ethanol and added to 1M (HS ...
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bioRxiv - Bioengineering 2022Quote: ... Tissues were homogenized by 3 × 10 s pulses at maximum speed with a VDI12 rotorstator (VWR, Dresden, Germany). Protein extracts were quantified by a Bradford protein assay (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mM glucose) (brain weight x 3) and 26% dextran (MW 504.4 g/mol) (#J14495.A1, VWR, UK) (brain weight x 4) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed 3 x with Tris buffered saline (TBS) with 1% bovine serum albumin (BSA; VWR) and 0.5% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 10 min in 1x PBS and mounted onto SuperFrost slides (VWR, #48311- 703) using VECTASHIELD HardSet Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Biochemistry 2024Quote: ... the sample was applied onto polyethersulfone membrane centrifugal filters with 3 kDa cutoff (VWR International GmbH, Darmstadt, Germany) to concentrate this fraction ...