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Citations for VWR :
351 - 400 of 8927 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Eosin (VWR) for 1 min ...
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bioRxiv - Genomics 2024Quote: Total RNA of minigene-transfected HEK293T cells and patient-derived fibroblasts was extracted using the peqGOLD Total RNA Kit (VWR Life Science). One microgram of RNA was treated with 1 U of DNaseI (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: The peqGOLD Tissue DNA Mini Kit (VWR Life Science) was used to extract genomic DNA after genome editing in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The sample was automatically processed in a NIC+ cartridge (S2 Genomics #100-215-389) by three 15 minute deparaffinization steps (CitriSolv, VWR), rehydrated by successive 1 mL washes of 100% ...
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bioRxiv - Genomics 2024Quote: ... then the supernatant was collected and filtered with a 0.45 µM syringe-filter (VWR 28143-312). Lentivirus was then stored at −80 °C in single-use aliquots.
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bioRxiv - Genomics 2024Quote: ... Samples were permeabilized in 1X PBS + 0.2% Tween-20 (VWR 100216-360) for 15 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... which were adjusted to a concentration of 1.25 × 106 cells/mL in iPS Brew + 10 µM ROCK inhibitor (Y-27632, VWR). 800 µL of cell suspension (approximately 1 million cells ...
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bioRxiv - Developmental Biology 2024Quote: ... tissue samples were taken from webbing on hindlimbs using single-use biopsy punches (VWR 21909-140). Heterozygous mating pairs were intercrossed to produce embryos for in situ hybridization and RNA-seq analyses ...
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bioRxiv - Developmental Biology 2024Quote: ... 15 scalpel (VWR, 82029-856) and Dumont No ...
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bioRxiv - Developmental Biology 2024Quote: ... we generated a sterile circuit by pumping 50 mL of medium from a 50 mL conical tube reservoir using a Masterflex Ismatec Reglo Independent Channel Control Peristaltic Pump (MFLX78001-80, 4-channel, 8-roller; Avantor/VWR, Radnor, PA, USA) fitted with 1.52 mm inner diameter 3-stop tubing (95625-36 ...
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bioRxiv - Developmental Biology 2024Quote: FuGene SI transfection reagent (VWR, # MSPP-SI-1000) was used for siRNA transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS-triton 0.8% (28817295, VWR) for 2 days at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with 10% absolute ethanol (20821.330, VWR Chemicals).
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were then rinsed with PBS 1x and washed 5 times with PBS-triton 0.8% (28817295, VWR) for 10 min with slight agitation ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were resuspended in 50μl Schneider’s Insect Medium and transferred onto chambered cell culture slides (VWR, 53106-306). The chamber slides were pretreated with 0.25mg/ml Concanavalin A (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... membranes were washed in 1x TBS-T and incubated for 1 hour at RT in the dark with species-specific secondary antibodies (Rb-680; 926-68071; Ms-800 926-32212) (VWR) at 1:10,000 ...
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bioRxiv - Developmental Biology 2024Quote: ... fixed in 4% paraformaldehyde (VWR) for 15-20 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... After 5 washes of 30 min with PBS-triton 0.8% (28817295, VWR), larvae were incubated with the secondary antibody using 1% goat serum (16210064 ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS-triton 0,8% (28817295, VWR) for 3 days at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... and kept for 30 min in PBS-triton 0.8% (28817295, VWR) on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS-triton 0.8% (28817295, VWR) for 3 hours at room-temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were rinsed twice with PBS-triton 0,8% (28817295, VWR) and kept for 30 min in PBS-triton 0.8% (28817295 ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.1mmol/l ß-mercaptoethanol and 15% (v/v) FBS (HyClone HYCLSH30088.03, VWR and SH30071.03, Cytiva) (M15Hy) ...
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bioRxiv - Immunology 2024Quote: ... 1 million CAR T cells for each condition were plated in 1mL fresh media (similar to their expansion condition) in a tissue culture treated 24 well-plate (VWR). After 24 hours ...
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bioRxiv - Immunology 2024Quote: ... then blocked with 1% casein (w/v) in PBS (VWR International, Radnor, PA) for 2 hours at room temperature with mild shaking (55 rpm) ...
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bioRxiv - Microbiology 2024Quote: ... a PerfectBlue Semi-Dry Electroblotter (Peqlab, VWR, Germany) was used at constant amperage for 1 h (150 mA for 8*6 cm membrane and 1.5 mm thick gel) ...
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bioRxiv - Genetics 2024Quote: ... A square 22 mm × 22 mm #1.5 coverslip (VWR, Cat# 16004-302) with a drop of Prolong Diamond Antifade Mountain with DAPI (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... 1 µl RNase A (VWR Life Science, Cat# E866-5ML), 6.25 µl DNA hybridization buffer (4 g Dextra sulfate sodium salt (Sigma Life Sciences ...
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bioRxiv - Genomics 2024Quote: ... and vortexed at speed 3 (∼600rpm) for 5 minutes (VWR Analog Vortex Mixer ...
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bioRxiv - Genomics 2024Quote: ... and vortexed at speed 3 (∼600rpm) for 5 minutes (VWR Analog Vortex Mixer; VWR, Radnor, PA, United States). The supernatant was transferred to a clean tube ...
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bioRxiv - Genomics 2024Quote: ... dissolved in DMSO or 0.5% DMSO solvent control (VWR). Ninety hours after transduction ...
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bioRxiv - Genomics 2024Quote: ... 2 mM CaCl2 (VWR International Ltd, 97062-820), and 42 mM MgCl2 (Sigma Aldrich ...
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bioRxiv - Genomics 2024Quote: ... 10% w/v dextran sulfate (VWR, 97062-828), 1 mg/mL yeast tRNA (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: ... filtered through a 40 µm Falcon cell strainer (VWR, 43-50040-51) into a 50 ml conical tube ...
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bioRxiv - Genomics 2024Quote: ... Nuclei were counted using a INCYTO C-chip disposable hemocytometer (VWR, 22-600-100).
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bioRxiv - Immunology 2024Quote: ... then transferred to a 1:1 solution of 30% sucrose and OCT (VWR, 95057-838) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... tubes with mAb were incubated on a rotator at 4 °C overnight on a rotator (Model 13916- 822, VWR, United States). These mAb included anti-histone H3 (1:50 ...
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bioRxiv - Genomics 2024Quote: ... supplemented with 10% fetal bovine serum (VWR), and 1% Pen/Strep solution (Gibco) ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred to nitrocellulose blotting membranes (10-6000-01, VWR) in Harlow buffer at 350mV and 4°C for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: ... The remainder ground tissue was boiled for 2.5 h at 100 °C before being neutralized with 5 mol.L-1 KOH (VWR, United States). Total glucose (free glucose + glucose produced by glycogen hydrolysis ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was homogenized in 1 mol.L-1 HCl (VWR, United States) and free glucose content was measured ...
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bioRxiv - Cell Biology 2024Quote: ... using the Bead Mill MAX Homogeniser (VWR). The steps of RNA and genomic DNA isolation can be found elsewhere in this manuscript ...
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bioRxiv - Cell Biology 2024Quote: Protein was collected by lysing worms in a 1:1 ratio of worm pellet to lysis buffer using a tissue grinder (VWR 62400-675) kept cold on ice ...
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bioRxiv - Cell Biology 2024Quote: ... The sections were placed on superfrost adhesion slides (VWR®). The following steps were performed according to the BaseScope™ Detection Reagent Kit v2 – RED User Manual ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed 1x with Dulbecco’s PBS (VWR, cat#02-0119-1000), and fresh media was added.
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bioRxiv - Cell Biology 2024Quote: ... Stock solutions of cycloheximide (VWR, 9427) were prepared fresh for each experiment ...
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bioRxiv - Cell Biology 2024Quote: ... Acetoxymethyl Ester (BCECF-AM) (VWR, 891390244) was prepared as a 100 µM stock solution in 10% DMSO in DPBS ...
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bioRxiv - Cell Biology 2024Quote: ... the appropriate area was shaved and disinfected using an iodine-based solution (BDH Prolabo, VWR International, Heverlee, Belgium). After surgery ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed in a Bio-Rad CFX96 machine using SYBR Green mastermix (VWR #101414) and qPCR primers listed in Table 2 ...
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bioRxiv - Cell Biology 2024Quote: ... the feature layer was either PDMS stamped onto bottomless 2mL cryovials (VWR #75852-324) or a custom 3D-printed reservoir layer produced out of high-temperature resin using a Formlabs 3D printer (Supplemental Figure 8) ...