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Citations for VWR :
301 - 350 of 1829 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... with the only difference that 2 ml of mineral oil (VWR) were placed on top of the sealed prep to facilitate temperature detection with a thermocouple ...
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bioRxiv - Microbiology 2020Quote: ... Protein syntheses were induced by 2% (wt/vol) galactose (VWR International) at 30 °C for 4 h at 250 rpm when the OD600 reached 0.8–1.0 ...
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bioRxiv - Microbiology 2019Quote: ... To an autoclaved 2 mL microcentrifuge tube (VWR, Radnor, PA, USA), 500 µL of the supernatant was added ...
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bioRxiv - Bioengineering 2019Quote: ... which had to be blocked in 2% (w/v) BSA (VWR) + 0,05% tween-20 in PBS to work (data not shown) ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in PBS + 2% SDS + protease inhibitor cocktail (VWR, #97063-972), and transferred to 2 mL disruption tubes (OPS Diagnostics ...
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bioRxiv - Cancer Biology 2021Quote: ... The fixed sections were covered with propan-2-ol (VWR, 20842312). Following evaporation for 40 seconds ...
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bioRxiv - Microbiology 2022Quote: ... The fungal specimen was sandwiched between 2 cover glasses (VWR international).
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bioRxiv - Neuroscience 2022Quote: The brain was transferred to 2% dimethyl sulfoxide (DMSO; VWR, USA) solution for cryoprotection for 1-2 days ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in 50 µl 2 M urea (VWR, 0568-500G), 50 mM ABC.
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bioRxiv - Neuroscience 2023Quote: ... Samples were run on a 2% agarose (VWR, Cat# MPN605-500G) and 1X Tris-Acetate-EDTA (TAE ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in 50 µl 2 M urea (VWR, 0568-500G), 50 mM ABC ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were washed 2 times with Histochoice Clearing Agent (VWR, H103) and 4 times with isopropanol (VWR,9084-03 ...
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bioRxiv - Immunology 2023Quote: ... and 2 steps at 50% with reagent alcohol (VWR BDH1156-4LP)) into water ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL of the labeled cell suspension was gently pipetted onto 22 x 22 mm No.1 cover glass (VWR) and sealed onto glass slides with clear acrylic nail polish for confocal imaging or onto Lab-Tek chambered #1 cover glass (Thermo Fischer Scientific)(for widefield fluorescence) ...
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bioRxiv - Immunology 2019Quote: ... cells were plated at a density of 1 x 105 cells/well (96-well plate) in complete RPMI (RPMI plus FBS (10%, VWR), L-glutamine (2mM ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from clarified cell culture supernatants using an Omega Total RNA Isolation Kit 1 (Omega BioTek, VWR) and reverse transcribed at 42°C using an oligo dT primer and SuperScript II reverse transcriptase (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... Cells on D-poly-Lysine coated coverslips were then fixed with pre-warmed 3.7% formaldehyde (v/v; VWR, VW3408-1), permeabilized with ice-cold methanol ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then subcultured 1:1000 into 7 mL of MSgg in a 6-well polyvinyl plate (VWR, PA, USA). Plates were incubated in static conditions at 30°C for 3 days and were then imaged using a Leica MZ10F macroscope and Leica DMC2900 camera ...
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bioRxiv - Cell Biology 2022Quote: B16-F1 cells were transfected with 0.5 µg EGFP-β-actin and 1 µL JetPrime transfection reagent (VWR, #114-07) per well in 6-well plates according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed in RPMI before resuspension in buffer (0.2% FBS in PBS) at concentration of 1 × 106 cells/ml in FACS tubes (Falcon® Brand, VWR). Before addition of the cocktail of 21 monoclonal antibodies ...
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bioRxiv - Neuroscience 2022Quote: The coverslip seeded with the transfected cells was placed in a custom glass-bottom chamber based on Chamlide EC (Live Cell Instrument) with glass bottom made with a 24 × 24 mm #1 coverslip (89082-270, VWR). Cells were continuously perfused with the external solution at ∼ 4 mL/min with a peristaltic pump (505DU ...
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bioRxiv - Microbiology 2023Quote: ... PBS was added to wells (1 mL) and the base and sides of each well were scraped twice using a cell scraper (VWR) to disrupt biofilm ...
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bioRxiv - Microbiology 2023Quote: ... The lentivirus-containing medium was then removed and cells were allowed to recover for 72 hours whereupon cells were passaged in the presence of 1 μg/mL puromycin (VWR) and remained under selection for 3 days.
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bioRxiv - Cancer Biology 2023Quote: ... 1×104 cells in 100μL media were plated in the top of the Corning filter membrane insert (VWR, Radnor, PA) sitting inside of a 24 well tissue culture plate ...
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bioRxiv - Microbiology 2023Quote: Samples were prepared by drying fixed bacterial cells spotted onto poly-L-lysine coated coverslips (VistaVision cover glasses, No. 1; VWR) in a 4°C refrigerator and subsequent dipping into water three times to dissolve precipitates from the growth media ...
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bioRxiv - Cell Biology 2021Quote: 3,000 ESCs/well mixed with 0.3 μg Wnt3a-beads/well were seeded onto human fibronectin (10 μg/mL, cat. um. 7340101, VWR/SLS) coated 8-well slides (cat ...
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bioRxiv - Microbiology 2019Quote: ... strains [69], were maintained in human foreskin fibroblasts (ATCC; Manassas, VA) in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA) supplemented with 10% fetal bovine serum (VWR) ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were enriched by negative selection using StemCell RosetteSep Human Monocyte Enrichment Cocktail (StemCell 15068; 75μl per ml of blood) and Ficoll-Paque (VWR 17144003). Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034 ...
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bioRxiv - Microbiology 2023Quote: Toxoplasma strain TATi-RHΔku80 [41] was cultured in human foreskin fibroblasts in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA, USA) supplemented with 10% fetal bovine serum (VWR) ...
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bioRxiv - Genetics 2021Quote: ... Resulting PCR products were loaded on a 2% agarose (VWR, 97062-250) gel ...
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bioRxiv - Immunology 2021Quote: ... 2 mL of HEPES-buffered saline pH 7.0 (2X for transfection) (VWR) was added dropwise to the previous mixture ...
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bioRxiv - Microbiology 2019Quote: ... cracked corns (2 g) were put in 20 mL scintillation vials (VWR) and rehydrated with 1 mL sterilized water overnight followed by autoclaving ...
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO). Acquisition of all samples was done on a BD FACSCelesta flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO).
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO). For the intracellular staining of infected U87.ACE2+ cells ...
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bioRxiv - Microbiology 2022Quote: All compounds (see supplemental table 2) were dissolved in DMSO (VWR V0231) and dispensed using an HP D300e digital dispenser in a 384-well plate format using a 2-fold dilution series ...
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bioRxiv - Bioengineering 2020Quote: ... Slices were then stained in Gill 2 hematoxylin solution (VWR: 10143-146) for 10 min ...
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bioRxiv - Genomics 2021Quote: ... to a 2 ml Phase Lock Gel Heavy tube (713-2536, VWR). The mixture was incubated for 5 min at 30°C at 1100 rpm ...
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bioRxiv - Immunology 2023Quote: ... overnight onto 96-well Immunlon-2 plates (VWR Scientific, Mississauga, ON, Canada). Recombinant protein antigens included SARS-CoV-2 FLS glycoprotein trimer [50 ng/well ...
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bioRxiv - Microbiology 2022Quote: ... samples were stored in PBS containing 2 % formaldehyde (VWR Life Science AMRESCO). Acquisition of all samples was done on a BD FACSCelesta flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... and crosslinked for 2 minutes using a UV Crosslinker (VWR, Radnor, PA). The blots were incubated with γ32-P 5’-labelled probe at 42°C overnight and imaged using a phosphor screen on a Typhoon FLA 7000 Variable Mode Imager System (GE Healthcare ...
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bioRxiv - Evolutionary Biology 2023Quote: ... This mixture was transferred into a 2 mm sterile electroporation cuvette (VWR) chilled on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... animals were soaked in 20 mg/mL 5-bromo-2’-deoxyuridine (VWR) with 3% DMSO in a 6 g/L Instant Ocean salt solution for 2 hours followed by a 4-hour chase prior to processing using the rapid protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... We confirmed the amplification using 2% Agarose I™ (VWR, Philadelphia, PA) and gel electrophoresis ...
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bioRxiv - Microbiology 2023Quote: All compounds (see supplemental table 2) were dissolved in DMSO (VWR V0231) and dispensed using an HP D300e digital dispenser in a 384-well plate format using a 2-fold dilution series ...
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bioRxiv - Cell Biology 2023Quote: ... samples were blocked with 2% bovine serum albumin (BSA, VWR, cat#97061), 1% DMSO ...
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bioRxiv - Microbiology 2023Quote: ... The mixture was then transferred into a 2 mm electroporation cuvette (VWR) and pulsed at 21 kV/cm ...
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bioRxiv - Microbiology 2024Quote: ... mechanically homogenized and afterward fixed with 2% Paraformaldehyde (10 min, RT; VWR). To minimize clumps ...
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bioRxiv - Biophysics 2023Quote: ... desalting with an ultracentrifuge filtration device (Vivacon 2 kD cut-off, VWR) was performed to remove residual Li+ cations and acetone.
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bioRxiv - Developmental Biology 2024Quote: ... with 2-4 coverslips (22 mm x 22 mm; VWR, 16004-094) on either side of the sample to avoid sample compression ...