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Citations for VWR :
301 - 350 of 1169 citations for Rat G Protein Coupled Bile Acid Receptor 1 GPBAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Fresh fecal pellets were obtained aseptically by direct defecation of rats into a sterile 120-ml container (VWR, Radnor, PA, USA). Samples were collected from the 153 rats at PD 60 ...
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bioRxiv - Cell Biology 2020Quote: ... Starvation medium contained 1,7g/L Yeast Nitrogen Base without amino acids and ammonium sulfate (#J630-100G, VWR, USA) and 2% glucose (#X997.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Trifluoroacetic acid, acetonitrile and 2-propanol were from Biosolve BV (Valkenswaard, The Netherlands) and glycine was from VWR International (Radnor ...
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bioRxiv - Cell Biology 2023Quote: ... temperature-mediated antigen retrieval was performed in citrate buffer (10 mM citric acid monohydrate (VWR, 20276.235, Pennsylvania, USA), Ph 6 ...
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bioRxiv - Physiology 2024Quote: ... cells were perfused with Ca2+-containing Krebs-Ringer’s solution supplemented with 0.1% fatty-acid free BSA (E588, VWR). To trigger ionic responses ...
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bioRxiv - Bioengineering 2020Quote: ... The M63 minimal media was prepared by diluting 15.6 g of M63 broth powder (VWR Life Science) in 0.9 L ultrapure water ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a centrifugation (10 minutes, 16.100 x g, 4°C, MicroStar 17R centrifuge, VWR, Darmstadt, Germany) for sample clearance ...
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bioRxiv - Biochemistry 2022Quote: ... A total amount of 15 μg protein was used for SDS-PAGE separation by electrophoresis and proteins were transferred onto nitrocellulose membranes (VWR, Lutterworth, UK). Even protein loading and transfer were confirmed by Revert 700 total protein staining (Li-COR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the proteins were diluted in 50 mM HEPES (VWR), pH 7.5 supplemented with 50 mM potassium sulphate (Honeywell ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: Protein extraction was performed with peqGOLD TriFast reagent (VWR), and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences) ...
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bioRxiv - Physiology 2021Quote: 6xHis fusion proteins were grown in M15 cells (VWR) and prepared as above except that after IPTG induction ...
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bioRxiv - Biochemistry 2023Quote: ... Staining was carried out with InstantBlue Protein Stain (VWR).
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined using the Bradford Assay (VWR). Protein lysates were diluted in RIPA buffer supplemented with 1× LDS and 1× DTT and heated at 95 °C for 5 minutes ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... A 5 mL syringe mounted on the infusion pump was connected to a swivel coupled with a spring leash (C313C; Plastics One; Roanoke, VA, USA) and Tygon® tubing (AAQ04103; VWR; West Chester, PA, USA) suspended over the chamber’s ceiling on a balanced metal arm ...
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bioRxiv - Microbiology 2021Quote: ... the lipoproteins removal was performed by mixing 150 µL of filtered plasma with 50 µL of milli-Q water and 10 µL of deuterated formic acid (0.1% final concentration) in a clean 10 kDa cut-off ultracentrifugation tube (VWR) using thermoshaker Eppendorf (10 min ...
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bioRxiv - Bioengineering 2021Quote: ... Wells were subsequently washed 4x with PBS-T and developed with 2,2-azinobis(3ethylbenzthiazolinesulfonic acid) (ABTS) (VWR 95059-146) substrate for 5 min at room temperature ...
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bioRxiv - Biophysics 2020Quote: Samples were vacuum dried and re-suspended in 6 µl of Buffer A (0.1% Formic acid (Roth) in MS grade water (VWR)).
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bioRxiv - Systems Biology 2023Quote: ... In the final steps the beads were resuspended in 20 μL 0.1 % formic acid in water and sonicated in an Ultrasonic Cleaner USC-T (VWR) for 10 min and the supernatant was transferred to a new plate ...
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bioRxiv - Microbiology 2023Quote: ... The composition of mobile phase A was 10 mM ammonium formate at pH 3.1 in 0.1% formic acid LC–MS grade (VWR Chemicals) and 10 % ultra-pure water (Merck KGaA) ...
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bioRxiv - Plant Biology 2024Quote: ... in H2OMilliQ (phase A) and 0.1% formic acid in acetonitrile (LC-MS grade; Fisher Scientific or HiPerSolv CHROMANORM, VWR) (phase B ...
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bioRxiv - Microbiology 2024Quote: ... Plates were then washed 5 times in PBS before addition of 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) substrate (VWR), and incubation at 37°C for 15 minutes ...
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bioRxiv - Plant Biology 2024Quote: Acetonitrile (MeCN), methanol (MeOH) and isopropanol were obtained from Biosolve (ULC grade, Valkenswaard, Netherlands) and formic acid from VWR Chemicals (LC–MS grade ...
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bioRxiv - Bioengineering 2020Quote: ... and buffer-exchanged to lower conductivity sucrose-dextrose dielectrophoresis buffer (DEP buffer: 2.39 g/L HEPES (VWR 3638C017), 80.7 g/L sucrose (Sigma-Aldrich S0389) ...
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bioRxiv - Microbiology 2019Quote: The phosphate buffer solution for NMR was prepared by dissolving 102 g of anhydrous KH2PO4 (VWR, Darmstadt, Germany), 0.5 g of TSP (3(trimethylsilyl ...
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bioRxiv - Pathology 2021Quote: ... The plate was centrifuged at 300 g for 10 min and fixed by directly adding paraformaldehyde (PFA, VWR) to the supernatant to a final concentration of 4% ...
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bioRxiv - Microbiology 2019Quote: ... Tissue cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, (VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
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bioRxiv - Microbiology 2022Quote: ... gondii co-cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
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bioRxiv - Microbiology 2022Quote: ... The filtrate was obtained by individually centrifuging 250 µl of each dilution at 5000 × g for 3 minutes through a 0.2 µm spin filter (Centrifugal Filter, Modified Nylon 0.2 μm, VWR). Both sample types ...
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bioRxiv - Microbiology 2023Quote: ... Mat samples (approximately 5 g) were homogenized in 15 mL disposable tissue grinders (VWR International, Radnor, PA, USA). Once ground ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged for 15 min at 14,000 × g through 10 kDa molecular weight cut-off filters (modified PES, VWR), and transferred into HPLC vials.
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bioRxiv - Neuroscience 2023Quote: ... 10 mM glucose) (brain weight x 3) and 26% dextran (MW 504.4 g/mol) (#J14495.A1, VWR, UK) (brain weight x 4) ...
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bioRxiv - Microbiology 2024Quote: ... The samples were centrifuged at 17,000 × g for 20 min and supernatants were sterilized using 0.2 µm cellulose acetate membrane filters (VWR) and kept at −20 °C until further analysis ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 μg virus envelope plasmid (VSV-G) using PEIPro DNA transfection reagent (#115010, VWR, Radnor, PA, USA). GFP-α-arrestins lentiviral particles were harvested 40 hr-post transfections ...
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bioRxiv - Molecular Biology 2021Quote: ... The elutes were incubated with S-protein agarose (VWR International) for 2 hours ...
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bioRxiv - Microbiology 2021Quote: Protein lysate was collected with RIPA buffer (VWR Life Science) supplemented with 2X protease inhibitors (Pierce ...
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bioRxiv - Cell Biology 2019Quote: ... 0.45μm low protein binding membrane (#28145-479) was from VWR International ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined with Bradford reagent (VWR, E530-1L). Western blotting was performed as previously described (43 ...
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bioRxiv - Immunology 2023Quote: ... Thereafter proteins were recovered using 10 kDa spin filters (VWR), and samples were reduced ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto a 0.45 µm nitrocellulose membrane (VWR), by electroblot ...
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bioRxiv - Biochemistry 2020Quote: Frozen hippocampus brain tissues were homogenized in a 4 volume of the 10% trichloroacetic acid (VWR International, Radnor, PA, USA) solution using a Kontes glass tissue grinder ...
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bioRxiv - Neuroscience 2022Quote: Peptides were solubilized in 6 μL buffer A (100% MS-LC grade water and 0.1% Formic acid (VWR Cat#84865.180), and a total volume of 3 μL were loaded onto a 30 cm-long column (75 μm inner diameter (Polymicro Cat#TSP075375) ...
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bioRxiv - Developmental Biology 2023Quote: ... We exposed the nucleic acids of each embryo with 5µl of QuickExtract™ DNA Extraction Solution (Lucigen, VWR, Philadelphia, PA), and incubated at 65°C for 15 minutes followed by 2 minutes at 98°C.
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bioRxiv - Immunology 2023Quote: ... Analytical grade ammonia solution (28%, GPR RECTAPUR®) and acetic acid glacial (AnalaR® NORMAPUR®) were obtained from VWR Chemicals.
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bioRxiv - Immunology 2023Quote: ... After the 10 minutes the reaction were stopped with 100 μL 0,5 M sulphuric acid (VWR Chemicals, cat no: 30144.294). The plates were read at 450 nm on an EL808 Ultra Microplate Reader (BioTek Instruments)
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bioRxiv - Microbiology 2023Quote: ... Then 20 μL of hemolymph per each sample were digested with 0.5 mL of 32% ultrapure hydrochloric acid (VWR Chemicals) under heating conditions (60 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Formic acid (FA), 2-proponol, acetonitrile (ACN), and methanol (all HiPerSolv CHROMANORM, HPLC grade, BDH Prolabo) were purchased from VWR International (Helsinki ...
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bioRxiv - Genomics 2020Quote: ... 13.4 g/ l (1.34% (w/v)) Yeast Nitrogen Base (Sunrise Science #1501-500) and 2% glycerol (VWR #AA36646-K7).
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bioRxiv - Cancer Biology 2019Quote: ... 100 µl of the cell suspension were injected using 25 G needles (BD Microlance TM 3. VWR 613-4952). Cell numbers and viability (required to be above 90% ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell supernatants were collected at different time points and centrifuged 30min at 10,000 × g at 4°C to eliminate cell debris (centrifuge CT15RE, VWR), then at 100,000 × g for 3h to eliminate larger vesicles (L7-55 Ultracentrifuge ...
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bioRxiv - Bioengineering 2021Quote: ... HI-FBS was then centrifuged at 100,000 x g for at least 16 h and the supernatant was filtered through a 0.20 μm bottle top filter (VWR) for subsequent use in BDMSC media.