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Citations for VWR :
251 - 300 of 336 citations for Goat Anti Rabbit IgG HRP H+L since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Bacterial load in faeces was determined using approximately 500 µL of frozen faecal slurry (238 – 816 mg) and diluting it 400,000 times in physiological solution (8.5 g/L NaCl; VWR International). Next ...
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bioRxiv - Cell Biology 2020Quote: Confluent monolayers of h-iECs in a 100-mm culture dish were subjected to orbital shear stress for 24 h at a rotating frequency of 150 rpm using an orbital shaker (VWR, Model 1000) positioned inside a cell culture incubator ...
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bioRxiv - Cancer Biology 2022Quote: Splenocytes were isolated from the spleen of 6-10-week-old OT-I male mice and pulsed with 2ug/ml of OVA peptide SIINFEKL (VWR, H-4866.0001BA) for 4 h in T cell culture media composed of RPMI1640 (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... and subsequently incubated at 37 °C and 5% CO2 for 2 h before seeded and imaged on an 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ (Gibco ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cultures expressing CcaS-CcaR system variants were cultured in darkness for 12-14 h in LB (1% Tryptone, 0.5% Yeast Extract, 1% Sodium Chloride) with appropriate antibiotics in 96 well plates (VWR, Cat#732-3802), with one 3 mm glass bead and 750 μl media per well at 37°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... reverse transcription was performed using 4.0 μl of 10.0 μl total RNA solution with the cDNA-Synthesis Kit H Plus (VWR Peqlab, Germany, 732-3273). CFX Connect™ Real-Time PCR Detection System (BioRad ...
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bioRxiv - Plant Biology 2019Quote: ... The test tubes were agitated on a shaker for about 24 h and the solution conductivity (C1) was measured with a conductivity meter (SR60IC, VWR, Radnor, PA). Leaf samples were then autoclaved at 120 °C for 30 min ...
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bioRxiv - Physiology 2020Quote: ... 5-μm sections were stained with hematoxylin and eosin (H&E) using the Leica Autostainer and mounted in DePeX (VWR, Lutterworth, UK). Adipocyte diameter and pancreatic β-cell islet number and size were quantified using ImageJ software as previously described (7 ...
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bioRxiv - Cell Biology 2023Quote: ... and twice with dH20 / 0.4% Kodak Photoflo and air dried prior to mounting with VECTASIELD with DAPI mounting medium (VWR #H-1200-10) and a VWR Micro Cover Glass ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in normal growth medium then switched to cysteine- and methionine-free medium DMEM supplemented with 2 mM L-glutamine (VWR) and 100 µM HPG (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... 50 µL of the collagen-I mixture was pipetted into the bottom of the wells of an 8-chamber slides (VWR) to coat the surface and allowed to gel for 20 minutes at 37°C ...
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bioRxiv - Microbiology 2021Quote: Gametocytes were produced by treatment of the mice at 1% parasitemia with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) in their drinking water for two days before exsanguination ...
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bioRxiv - Cell Biology 2021Quote: ... 20 μl of cells were put to 180 μl of TYI-S-33 media in 96-wells Tissue Culture Plate (VWR) and the same dilution step was performed 5 times ...
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bioRxiv - Cell Biology 2022Quote: ... The integrated vector backbone was removed by growth in non-selective YT media (5 g/l yeast extract (VWR, EM1.03753.0500), 5 g/l tryptone (Fisher Scientific ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... 200 µl of this resuspended culture was aliquoted into a 96-well black/clear glass bottom sensoplate (Greiner Bio-one VWR). Four technical replicas were measured per culture ...
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bioRxiv - Evolutionary Biology 2022Quote: ... yeast cultures were diluted to OD600=0.67 in 5 mL SGDCAA (6.7 g/L YNB without amino acid VWR #90004-150), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Each cell aliquot was recovered in 1 mL outgrowth media at 37°C for 1 hr and then transferred into 100 mL of molten LB (1% tryptone, 0.5% yeast extract, 1% NaCl, 100 g/L ampicillin (VWR # V0339), 0.4% SeaPrep agarose (VWR ...
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bioRxiv - Microbiology 2022Quote: ... albicans were cultured at 37 °C with aeration in 5 mL of YPD (10g/L Yeast Extract (VWR J850-500G), 20g/L Peptone (VWR J636-500G) ...
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bioRxiv - Microbiology 2023Quote: ... Single-species cultures were obtained by picking colonies using 1 µL sterile inoculation loops and transferred to 800 µL liquid isolation medium in 96 deep well 2 mL plates (VWR) which were incubated for at least 2 weeks on a bench top shaker (RT ...
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bioRxiv - Microbiology 2023Quote: Samples were prepared by drying fixed bacterial cells spotted onto poly-L-lysine coated coverslips (VistaVision cover glasses, No. 1; VWR) in a 4°C refrigerator and subsequent dipping into water three times to dissolve precipitates from the growth media ...
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bioRxiv - Microbiology 2024Quote: ... The main culture was cultivated as 10x 500 mL cultures in 2 L shake flasks sealed with breathable rayon film (VWR) for reproducible aeration for 72 h ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 24 h starved polyps were incubated in 1 mM linalool for 10 min in a 60 mm tissue culture dish (VWR International, Radnor, PA). Each animal was pinched using a pair of Dumont No ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with 250 nM TG or 10 µM monensin in fresh medium for 0.5 h at 37°C before 40 µM D-biotin (VWR Life Science, Radnor, PA) was added ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Microbiology 2021Quote: ... coli 0.1229 and its derivatives were harvested after 16 h shaking at 37°C and passed through 0.2 µm cellulose acetate filters (VWR Life Sciences, Radnor, PA). Assays to quantify Stx amplification were performed as previously described (15) ...
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bioRxiv - Immunology 2022Quote: ... The diffusion ring through the agarose gel containing mono-specific antibody after 24 h of incubation at room temperature was measured using a caliper with a precision of 0.1 mm (VWR traceable caliper; Radnor, PA). The values of the sample’s ring were read off the standard curve to determine Ig concentrations in g/L ...
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bioRxiv - Cancer Biology 2024Quote: 5×106 MM.1Sgfp+/luc+ cells were treated with DMSO or 1.00 μM TriC for 24 h in T-25 flasks (Avantor/VWR; Cat. no. 10861-568). Cells were then harvested ...
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bioRxiv - Neuroscience 2021Quote: ... In short, free-floating sections were blocked (5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) and then incubated with different primary antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... Stained tissue sections were washed in 0.1% Triton-X/PBS at RT for 1 hour and mounted on a poly-L-lysine coated coverglass (VWR, 48393-241) for imaging (Fig ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to 200 µl of 50% HNO3 and heated for 2 hrs at 90°C in a loosely capped 15ml centrifuge tube (VWR, #490001), (3 ...
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bioRxiv - Microbiology 2020Quote: ... Two-µl spots of each dilution were plated on selective (20 µg/mL thiamphenicol) and unselective square (90×15 mm, VWR international) BHI plates with or without 200 ng/mL anhydrotetracycline (ATc) ...
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bioRxiv - Microbiology 2020Quote: ... we used 24.4 ± 1.7 mM fructose (146.4 ± 10.3 mmol C L−1) and the following carboxylates at a concentration of 108.2 ± 8.0 mmol C L−1: Na-acetate (VWR, Solon, OH, USA), Na-butyrate ...
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bioRxiv - Genetics 2020Quote: ... the cells were resuspended in 1 ml of sterile water and spread on a plate containing minimal media lacking the corresponding amino acid or nucleotide (YNB or Synthetic Complete (SC): 6.7 g/l yeast nitrogen base (VWR 97064-162), 20 g/l glucose ...
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bioRxiv - Neuroscience 2021Quote: ... mice were deeply anaesthetized with isoflurane (4% flow rate of 0.5–0.7 l/min) and transcardially perfused with cold phosphate-buffered saline (PBS) followed by 4% PFA (VWR International Inc, 15710). Brains were extracted and placed in PFA for 24 h and then transferred to 30% sucrose/PBS solution at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The 5 L bioreactors were grown for one to two weeks pl shredded (VWR 200 Homogenizer on high for one minute) and transferred to 12L bioreactors ...
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bioRxiv - Immunology 2021Quote: ... 2mM l-glutamine and 25mM HEPES) were incubated for 96 hours at 37°C either on a 360° rotator (VWR International) or standing stationary (as specified) ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii cultures were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii strains were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
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bioRxiv - Neuroscience 2021Quote: ... Ribbons were deparaffinized by heating at 65°C for 1 h followed by washes in histoclear I and II (VWR; 101412-876, 10141-8822) for 5 m ...
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bioRxiv - Developmental Biology 2022Quote: ... The primary and the secondary antibodies were prepared in the blocking agent consisting of 0.1% Triton X-100 and 3% goat serum (VWR). Followed by washing the organoids with PBS 3x5min on a rocker ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT on a rocker with a blocking buffer consisting of 0.1% Triton X-100 and 5% goat serum (VWR). The organoids were incubated overnight in the primary antibodies (File S6 ...
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bioRxiv - Molecular Biology 2021Quote: ... Stellaris® RNA FISH Wash Buffer B was removed and samples were resuspended in Vectashield Antifade Mounting Medium with DAPI (VWR; Cat #: H-1200-10). Samples were then placed on a slide and sealed with a coverslip and nail polish.
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bioRxiv - Bioengineering 2021Quote: ... A 15-µl aliquot of the master mix was combined with 10 µl of sample in a 96-well PCR plate (VWR, 83007-374). The plate was mixed on a Qiagen TissueLyser II for two 25-second intervals at 13 Hz then centrifuged for 2 minutes at 1000 xg.
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bioRxiv - Microbiology 2022Quote: ... the mice were kept on standard drinking water or were provided water supplemented with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) for two days before the infectious blood meal to select for gametocytes ...
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bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Neuroscience 2023Quote: ... and BHK-B19L cells (see above) were plated in 10 cm plates coated in poly-L-lysine in normal medium (10% fetal bovine serum (VWR 16777-014) and antibiotic-antimycotic (Thermo 15240096 ...
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bioRxiv - Cell Biology 2023Quote: ... The stained tissue sections were washed in 0.1% Triton-X/PBS at RT for 1 hour and finally mounted on poly-L-lysine coated cover glasses (VWR, 48393-241) for triple-view line confocal super-resolution imaging20.
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bioRxiv - Cancer Biology 2023Quote: ... NAC-containing water was prepared using reverse osmosis filtered tap water and granulated N-acetyl-L-cysteine (>99%, MilliporeSigma Canada, Oakville ON) and was sterilized using 0.2 um PES filter units (VWR Canada, Mississauga ON). The prepared water was stored for a maximum of 2 months before use ...