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Citations for VWR :
251 - 300 of 415 citations for Anti Rat IgG H+L Antibody Conjugated to HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Hippocampal neurons seeded at 180,000 cells/well onto 0.1 mg/mL poly-L-lysine coated coverslips (#1.5, VWR) in 6-well plates ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 ml cultures were grown overnight and used to inoculate 1 L of terrific broth media (VWR). Cells were grown until they reached an OD of 0.8 and then cooled on ice before transferring to a 16 °C incubator for overnight induction using 0.5 M IPTG (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... The growth experiments were conducted in 1-L DURAN pressure plus bottles (VWR International, LLC, Darmstadt, Germany), which were inoculated with cultures that had been precultured in 50 mL RCM and then in 100 mL PETC medium with fructose ...
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bioRxiv - Immunology 2024Quote: ... and cells were transferred to medium (RPMI 1640 with L-glutamine and 25 mM HEPES; VWR International), supplemented with 5% human AB serum (Gemini Bio) ...
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bioRxiv - Biophysics 2020Quote: ... the untreated and treated bacteria (expressing H-NS-mEos3.2 fusion proteins) were filtered by 0.2 μm filters (VWR International LLC), resulting in filtrates constituting leaked cellular contents due to membrane damage ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 mL 5% PVA (w/v) in 200 mM sucrose was incubated for 1 h at 90°C in a thermal shaker lite (VWR international GmbH ...
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bioRxiv - Cell Biology 2024Quote: ... The remainder ground tissue was boiled for 2.5 h at 100 °C before being neutralized with 5 mol.L-1 KOH (VWR, United States). Total glucose (free glucose + glucose produced by glycogen hydrolysis ...
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bioRxiv - Cell Biology 2024Quote: ... 1:1000 LIF) and were pre-plated for 1-2 h to remove fibroblasts before they are seeded on matrigel (VWR) coated dishes ...
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bioRxiv - Molecular Biology 2022Quote: ... The lentivirus-containing medium was harvested 72 h post-transfection by filtration through a 0.45 μm polyethersulfone (PES) filter (VWR Sciences). The filtered lentivirus was aliquoted ...
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bioRxiv - Cell Biology 2024Quote: ... The lentivirus-containing medium was harvested 72 h post-transfection by filtration through a 0.45 μm polyethersulfone (PES) filter (VWR Sciences). The filtered lentivirus was distributed in aliquots ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate number of acinar cells were then resuspended in premade collagen solution (1 mg/ml rat tail type1 collagen (VWR, Cat. No. CACB354236), 10× Waymouth’s media (Cedarlane Labs ...
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bioRxiv - Cancer Biology 2022Quote: ... which consisted of RPMI-1640 medium supplemented with 1% Ultra Low IgG Fetal Bovine Serum (VWR 4100-050) and added to the cell-containing wells ...
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bioRxiv - Cell Biology 2020Quote: ... Starvation medium contained 1,7g/L Yeast Nitrogen Base without amino acids and ammonium sulfate (#J630-100G, VWR, USA) and 2% glucose (#X997.5 ...
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bioRxiv - Bioengineering 2020Quote: ... and buffer-exchanged to lower conductivity sucrose-dextrose dielectrophoresis buffer (DEP buffer: 2.39 g/L HEPES (VWR 3638C017), 80.7 g/L sucrose (Sigma-Aldrich S0389) ...
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bioRxiv - Physiology 2021Quote: ... Cryostat-cut sections (8-10μm) were mounted directly onto poly-L-lysine-covered glass slides (VWR, Leuven, Belgium) by the Institute of Metabolic Science Histology Core ...
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bioRxiv - Synthetic Biology 2021Quote: ... HEK293T and HEK293TERK2-EGFP cells were cultured in DMEM media supplemented with high glucose and L-glutamine (VWR) supplemented with 10% Hyclone FetalClone I serum (VWR ...
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bioRxiv - Plant Biology 2020Quote: ... USA) and then moved into a forced-air oven (VWR Oven F Air 104 L, VWR, Radnor, PA) at 65 °C to dehydrate ...
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bioRxiv - Genetics 2020Quote: ... nos-Cas9/nos-Cas9 (Fig. 4A) males in 0.3 L plastic bottles (VWR® Drosophila Bottle 75813-110). Parent (P ...
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bioRxiv - Microbiology 2022Quote: ... gondii co-cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
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bioRxiv - Bioengineering 2024Quote: ... Chondrocytes were cultured in Dulbecco’s modified Eagle’s medium (DMEM, no glucose, L-Glutamine, and sodium pyruvate; VWR, 11966025) with 10% fetal bovine serum (R&D Systems ...
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bioRxiv - Microbiology 2023Quote: ... The end of the Tongue Cleaner was placed in a sterile vessel containing 2mL phosphate-buffered saline (PBS, pH=7.4, M/L= 1.37M sodium chloride, 0.27M potassium chloride, 0.1M phosphate buffer; VWR, Lutterworth, UK) and 1g of 3.5-5.5mm sterile glass beads (VWR ...
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bioRxiv - Cell Biology 2023Quote: ... Embryonic hippocampi were dissociated using papain and seeded at 180,000 cells/well for microscopy experiments on 0.1 mg/mL poly-L-lysine coated wells or glass coverslips (#1.5, VWR) in 6-well plates ...
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bioRxiv - Developmental Biology 2022Quote: ... H&E routine stain was performed using Leica ST5010 Autostainer XL and Leica ST Infinity H&E Staining System kit (VWR#3801698) according to the manufacturer’s instructions ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Coating was performed for 1 h with 15 μg/ml of fibronectin or with 3.5 μg/cm2 Cell-Tak (VWR, 734-1081) diluted in 0.1 M sodium bicarbonate ...
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bioRxiv - Biochemistry 2020Quote: ... The 168 h incubation samples were sonicated for 2 minutes in a water bath sonicator (VWR® Symphony™ Ultrasonic Cleaner). Circular dichroism (CD ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to a PVDF membrane for 1.5 H at 400 mA and blocked using 5 % BSA (VWR, 103219-864) rocking at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... PNA-FISH hybridization for rat wound sections was further performed following a similar protocol but with hybridization conducted on UltraFrost adhesion slides (VWR international, 50-301-70). Paraffin-embedded punch biopsy sections from control and 5mW/cm2-treated wounds were placed on top of the UltraFrost adhesion slides followed by hybridization inside a temperature-controlled oven (57°C for 120 minutes) ...
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bioRxiv - Genomics 2020Quote: ... and Avadin-FITC antibodies (VWR CAP21221) in 10 μg/ml BSA ...
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bioRxiv - Genomics 2020Quote: ... 13.4 g/ l (1.34% (w/v)) Yeast Nitrogen Base (Sunrise Science #1501-500) and 2% glycerol (VWR #AA36646-K7).
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bioRxiv - Bioengineering 2020Quote: ... then cells were homogenized and plated in B27-containing Neurobasal medium on Poly-L-Lysine treated #1.5H glass coverslips (Marienfeld, VWR) to a density of 4000 cells per cm2 ...
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bioRxiv - Immunology 2021Quote: ... containing amphibian-adjusted Leibovitz’s L-15 (AL-15; Wisent Inc.) medium supplemented with 15% fetal bovine serum (FBS; VWR), herein referred to as Xela complete media ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we thawed yeast RBD library and the Wuhan Hu-1 and Omicron BA.1 strains by inoculating the corresponding glycerol stocks in SDCAA (6.7 g/L YNB without amino acid [VWR #90004-150] ...
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bioRxiv - Microbiology 2023Quote: The pH measurement of all samples was performed using a pH electrode (VWR® PHenomenal® PH 1000 L). Before performing the measurement ...
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bioRxiv - Microbiology 2024Quote: Bacterial load in faeces was determined using approximately 500 µL of frozen faecal slurry (238 – 816 mg) and diluting it 400,000 times in physiological solution (8.5 g/L NaCl; VWR International). Next ...
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bioRxiv - Microbiology 2024Quote: ... blood was collected by cardiac puncture into incomplete RPMI with 25mM HEPES and L-Glutamine (VWR Cat# 45000-412) at 37°C and enriched by an Accudenz discontinuous gradient as described above ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Tryptic peptides were resolubilized in 30 μl of 0.05% TFA and 2% ACN in H2O filtered through 10 kDa MWCO PES membrane spin filters (VWR) at 14,000×g for 15 min and transferred to HPLC vials.
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bioRxiv - Synthetic Biology 2023Quote: ... 10 mL of seed culture was used to inoculate 1 L media in a Fernbach flask (VWR 29171-854). Cells were incubated at 37 °C shaking at 210 rev/min ...
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bioRxiv - Microbiology 2023Quote: ... Sorbarod filter samples were homogenised via placement in half-strength thioglycolate medium (14.5 g/L; Oxoid, Basingstoke, UK) and 1g of 3.5-5.5mm sterile glass beads (VWR, Lutterworth, UK). Samples were vortexed (30 seconds) ...
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bioRxiv - Microbiology 2024Quote: ... at 20,000 × g for 1 h at 4 °C and resuspended in 10% (v/v) of the start volume with cold TN50 buffer (1.21 g/L Tris-HCl (VWR), 2.92 g/L NaCl (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Isolates were stored at - 80°C in freezing medium (-Nutrient broth (Oxoid CM0067) 21,25 g/L (2,13%)-Glycerol 85% (VWR 24384) 184,5 g/L (=150 mL/L ...
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bioRxiv - Biophysics 2024Quote: Glass-bottom 384-well plates (Brooks, MatriPlate MGB101-1-2-LG-L) and cover glass (VWR, USA, 48393-251) were passivated with PEG-silane (MW = 5 kD ...
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bioRxiv - Cell Biology 2020Quote: Confluent monolayers of h-iECs in a 100-mm culture dish were subjected to orbital shear stress for 24 h at a rotating frequency of 150 rpm using an orbital shaker (VWR, Model 1000) positioned inside a cell culture incubator ...
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bioRxiv - Cancer Biology 2022Quote: Splenocytes were isolated from the spleen of 6-10-week-old OT-I male mice and pulsed with 2ug/ml of OVA peptide SIINFEKL (VWR, H-4866.0001BA) for 4 h in T cell culture media composed of RPMI1640 (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... and subsequently incubated at 37 °C and 5% CO2 for 2 h before seeded and imaged on an 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ (Gibco ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cultures expressing CcaS-CcaR system variants were cultured in darkness for 12-14 h in LB (1% Tryptone, 0.5% Yeast Extract, 1% Sodium Chloride) with appropriate antibiotics in 96 well plates (VWR, Cat#732-3802), with one 3 mm glass bead and 750 μl media per well at 37°C ...
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bioRxiv - Physiology 2020Quote: ... 5-μm sections were stained with hematoxylin and eosin (H&E) using the Leica Autostainer and mounted in DePeX (VWR, Lutterworth, UK). Adipocyte diameter and pancreatic β-cell islet number and size were quantified using ImageJ software as previously described (7 ...
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bioRxiv - Cell Biology 2023Quote: ... and twice with dH20 / 0.4% Kodak Photoflo and air dried prior to mounting with VECTASIELD with DAPI mounting medium (VWR #H-1200-10) and a VWR Micro Cover Glass ...
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bioRxiv - Bioengineering 2024Quote: ... cryosections (CryoStar NX70 Cryostat) were taken at 10 μm thickness and stained with hematoxylin and eosin (H&E) (VWR, 95057-844, -848) according to the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Blots were washed with 1x TBS with 0.1% Tween-20 (TBST) and blocked using 5% IgG-free BSA (VWR RLBSA50). Primary antibodies (Supplemental Table 2 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were cultured in normal growth medium then switched to cysteine- and methionine-free medium DMEM supplemented with 2 mM L-glutamine (VWR) and 100 µM HPG (Invitrogen) ...