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Citations for VWR :
251 - 300 of 903 citations for 4 Chloro 2 fluoro 3' 4 methylpiperazinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The cell lysate was separated from insoluble material by centrifugation at 40,000 g for 60 minutes at 4 °C and subsequently incubated with a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C on a rotator for affinity chromatography via the 6xHis-tag ...
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bioRxiv - Plant Biology 2022Quote: ... 0.4 g of poly(FATRIFE-ter-PEGMA-ter-NBMA) terpolymer was dissolved in 25ml methyl-ethyl ketone (25642.325, VWR, UK) in a 500 ml rotary evaporator flask containing 20 g of FEP at 40 °C to coat FEP particles ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated at 4℃ to maintain their viability and passed through a 40 µm cell strainer (VWR, 76327-098) to dissociate single cells from clumps ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Cell Biology 2021Quote: ... After solidification of the gelatin block for 6h at 4°C it was embedded in a larger OCT block (Qpath, VWR) and subjected to cryosectioning (10 µm ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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bioRxiv - Cell Biology 2020Quote: Melanocytes were cultured in 10-cm dishes and fixed in situ with Karnovsky’s fixative [4% paraformaldehyde (VWR Scientific, Radnor, PA), 4 mM calcium chloride ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 4-12% Bis-Tris precast gels (Fisher Scientfic, WG1402BOX) and then transferred to 0.45μM nitrocellulose membranes (VWR, 10120-006). Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... washed once in ice-cold 1× PBS with Ca2+/Mg2+ and then fixed in 4% paraformaldehyde (PFA, VWR: 100504-940) for 30 mins at room temperature ...
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bioRxiv - Physiology 2024Quote: ... were then exposed to cold treatments of -15°C for up to 48 hours (in increments of 4, 8, 12, or 48 hours) in a cooling circulator (VWR Scientific 1167P Heating/Cooling Recirculating Water Bath ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Jag1-Fc was immobilized by overnight incubation at 4°C in individual wells of non-tissue culture-treated 6 or 12 well plates (VWR) at a final concentration of 2 µg/mL in DPBS containing 10 µg/mL poly-D-lysine (ThermoFisher Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Blood plasma was obtained by centrifuging at 5000 rpm for 10 minutes at 4 °C and stored in cryotube vials (VWR Micro-centrifuge tubes with graduation 2.0 mL ...
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bioRxiv - Bioengineering 2023Quote: The strains were grown overnight in M9 minimal media (4 g/L glucose, 1 g/L casamino acids (cat. no 2240, VWR), and 1 mg/L thiamine (cat ...
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bioRxiv - Biophysics 2023Quote: HeLa cells were fixed using 4% paraformaldehyde and permeabilized using Triton-X100 (VWR, Radnor, PA, USA, Cat. No. M143-1L) 12 hours after TTLL6-YFP transfection ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed by adding 100 µl 8% PFA for 20 min at RT (final concentration of PFA is 4%; PFA from VWR). All media was then gently removed and cells were washed 3 times for 5 minutes with 150 µl PBS ...
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bioRxiv - Microbiology 2024Quote: ... then pelleted at 3200 x g for 10 minutes at 4°C and washed twice with pre-chilled blood bank saline (89370-096, VWR). Pellets were resuspended in 0.8 mL 3:1:0.004 acetonitrile:methanol:formic acid + 10 nM verapamil (V105 ...
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bioRxiv - Physiology 2024Quote: ... MPCs were allowed to differentiate for 5 days before adding differentiation medium supplemented with 50 nM 4-OH TAM (89152-604, VWR) for 48 hrs to induce recombination in HSA-miR-1-derived myotubes (or for TAM control in WT-derived myotubes) ...
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bioRxiv - Genomics 2024Quote: ... The suspension containing heads was poured through a 100 µm cell strainer to separate tissue from the collection sucrose buffer and separated into 4 equally aliquots that were each separately homogenized with 7 gentle strokes in a KONTES Tissue Grinder (VWR) (loose pestle ...
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bioRxiv - Developmental Biology 2024Quote: Embryonic tissue samples from different embryonic days were fixed overnight at room temperature in 4% paraformaldehyde solution (VWR, Pennsylvania, USA), followed by dehydration and embedding in paraffin ...
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bioRxiv - Developmental Biology 2024Quote: ... 20 to 30 animals per treatment were incubated at 4°C for 1 hour and then transferred into an electroporation cuvette (Gene Pulser/Micro Pulser Electroporation Cuvette 0.4-cm gap, Bio-Rad or electroporation cuvettes 4-mm gap, VWR) and washed twice with chilled Milli-Q water ...
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bioRxiv - Physiology 2020Quote: ... adi14-3-3ζKO mice were fasted for six hours and challenged with d-glucose (2 g/kg b.w.; VWR, Solon, OH) by intraperitoneal administration (34 ...
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bioRxiv - Bioengineering 2020Quote: ... The PDMS slab was bonded to a 2-in by 3-in glass slide (VWR micro slides, cat. #48382-179) after plasma treatment (Harrick Plasma PDC-001 ...
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bioRxiv - Microbiology 2021Quote: ... The PDMS slab was bonded to a 2-in by 3-in glass slide (VWR micro slides, cat. #48382-179) after plasma treatment (Harrick Plasma PDC-001 ...
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bioRxiv - Biophysics 2021Quote: ... The sonicator probe (SFX250 Sonifier with 1/2” horn and 3 mm tapered microtip, Branson Ultrasonics, VWR International, Radnor PA) was then positioned in the chamber with its tip 2 mm above the coverslip ...
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bioRxiv - Synthetic Biology 2024Quote: ... At least 3 colonies per strain were picked and inoculated in 2 mL-deep 96 well plates (VWR 76397-576) with 1 mL of MPG supplemented with 50 μg/mL ampicillin and 100 μL of 20% dextrose ...
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bioRxiv - Microbiology 2021Quote: ... ‘Fast’ shaking was established by orbital rotation of flasks at setting 4 on an orbital shaker (Model 3500, VWR, Radnor, PA), which corresponds to ∼200 rpm ...
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bioRxiv - Cancer Biology 2021Quote: ... Matrigel containing organoid samples were removed from multi-well tissue culture dishes (using a 3.5 mL disposable transfer pipette and placed in 45 mL of cold PBS (4°C) in 50 mL capacity polypropylene centrifuge tubes (VWR Scientific). Matrigel was washed away by 3-4 rounds of suspension in cold PBS and centrifugation using a refrigerated centrifuge set to 1,000 pm ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted at 5,000 rpm for 15 minutes at 4°C and resuspended in 10 mL of lysis buffer [300 mM NaCl (VWR BDH9286), 20mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2021Quote: ... mice were deeply anaesthetized with isoflurane (4% flow rate of 0.5–0.7 l/min) and transcardially perfused with cold phosphate-buffered saline (PBS) followed by 4% PFA (VWR International Inc, 15710). Brains were extracted and placed in PFA for 24 h and then transferred to 30% sucrose/PBS solution at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... After overnight plating cells were treated with different doses of CDDP for 24 hours or with MMS for 8 hours and fixed with 4% PFA (VWR, AA47377) for 20 minutes and stored in PBS at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... tubes with mAb were incubated on a rotator at 4 °C overnight on a rotator (Model 13916- 822, VWR, United States). These mAb included anti-histone H3 (1:50 ...
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bioRxiv - Bioengineering 2023Quote: ... conditioned media was centrifuged at 3000 RPM for 10 minutes at 4°C and the supernatant was filtered using 0.2 μm syringe filter (VWR, 28143-310). Sample placed in ultracentrifuge tubes (Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... between the starting and ending levels of the removed capillary tubes were measured using a digital caliper (Traceable Carbon Fiber Caliper-4″, VWR International) to determine the volume consumed (after adjusting for evaporative losses) ...
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bioRxiv - Physiology 2023Quote: ... the sulfhydryl groups were derivatized by transferring the final supernatant to 1.55 M NaOH and Na2B4O7·10H2O (125 mM with 4 mM EDTA, pH 9.5, VWR, Radnor, PA, USA) solutions ...
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bioRxiv - Immunology 2024Quote: ... Plaque assays were developed at 4 days post infection by removal of overlay medium and staining/fixation using 10% neutral buffered formalin (VWR, #89370) and 0.25% crystal violet (VWR ...
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bioRxiv - Immunology 2024Quote: ... Gametes were washed with PBS and incubated with mAbs dilutions in PBS containing 0.5% PBS and 0.05% NaN3 (SIFA buffer) for 1 hr at 4 °C in sterile V bottom plates (VWR#736-0223). After incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... overnight at 4°C followed by a goat anti-rabbit IgG secondary antibody conjugated to HRP (VWR, cat. no. 102645-182). F1β signal was then captured using the same method.
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bioRxiv - Biophysics 2024Quote: ... at 4°C overnight in a 35 mm Mat-Tek glass coverslip bottom dish (VWR; Catalog No. P35G-0-14-C). After the poly-D lysine coating ...
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bioRxiv - Cell Biology 2024Quote: ... cells were harvested and sonicated with a Branson SFX250 Sonifier with 102-C Converter and a 3/4" High-Gain Horn in cold lysis buffer containing 50 mM sodium phosphate-NaOH pH 7.5 (Oakwood #099682, VWR #97064-480), 150 mM NaCl (Millipore #1.06404) ...
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bioRxiv - Genetics 2024Quote: ... individuals were fixed for 1 hour in 1×PBS and 4% paraformaldehyde at room temperature, washed with BBT (1×PBS, 0.1% Tween 20 (VWR, ref:28829.296), 0.1% BSA (Euromedex ...
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bioRxiv - Cell Biology 2020Quote: ... 3% glucose (VWR), 3.5% active dried yeast (SLS) ...
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bioRxiv - Immunology 2021Quote: ... A single incision was made above the quadriceps and a 2 mm or 3 mm biopsy punch (VWR, 21909-132, −136) was used to make a fullthickness muscle defect ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cells were resuspended in 65μL RNAase A solution consisting of 10 mg/mL RNAase A (VWR Life Science, 9001-99-4) dissolved in 10 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... Then the tissues were washed in PBS and stored at 4°C with 0.025% (w/v) sodium azide (VWR, Cat#786-299). For cryoprotection ...
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bioRxiv - Biophysics 2020Quote: VLPs suspended in PBS buffer were mixed at 4°C with KIF5B-spycatcher in (buffer) for 7-8 hours on a nutating mixer (VWR, Radnor, PA) to ensure Spy Tag-Spy Catcher binding ...
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bioRxiv - Neuroscience 2021Quote: ... Rats were then perfused intracardially with 100ml of 0.9% phosphate saline buffer (PBS) and 100ml of ice-cold 4% paraformaldehyde (PFA, BDH, Prolabo, VWR International, Lovaina, Belgium). Then ...
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bioRxiv - Physiology 2021Quote: ... Tissue samples were embedded in paraffin and sections of 4 μm were coated with 43–45°C Kodak NTB emulsion (VWR, Herlev, Denmark) diluted 1:1 with 43–45°C water ...
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bioRxiv - Neuroscience 2022Quote: ... Then incubated in 10% and 30% sucrose in PBS for 24 hr sequentially at 4 °C and embedded in the OCT compound (cat# 4583; VWR, Randor, PA). Coronal sections were cut at 20 or 50µm on a cryostat (CM3050S ...
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bioRxiv - Neuroscience 2022Quote: ... Rats were then perfused intracardially with 100 ml of 0.9% phosphate saline buffer (PBS) and 100 ml of ice-cold 4% paraformaldehyde (PFA, BDH, Prolabo, VWR International, Louvain, Belgium). Then ...