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Citations for VWR :
201 - 250 of 1038 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... The primary and the secondary antibodies were prepared in the blocking agent consisting of 0.1% Triton X-100 and 3% goat serum (VWR). Followed by washing the organoids with PBS 3x5min on a rocker ...
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bioRxiv - Microbiology 2022Quote: ... was diluted 1:3 in cold PBS and 100 μL was added to 0.4 μm polycarbonate trans-well plate inserts (VWR). The Transwell plates were placed 4°C for 30 min to let the Matrigel settle and were then moved to an anaerobic chamber to gel at 37°C for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... and DNA was precipitated by adding 1/10th volume 3 M sodium acetate pH 5.2 and 1 volume isopropanol (3032-16, VWR) then incubating at 22°C for 18 hr ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Neuroscience 2023Quote: ... all odors in this study were presented from a 10-3 (0.1%) dilution in paraffin oil (J.T. Baker, VWR #JTS894), with the exception of movies collected from one fly ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was washed 3 times in PBS before blocking for 30 minutes room temperature with PBS+0.1% Triton X-100 (VWR) with 5% donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2024Quote: Purified chromosomes 3 or 19 were cytocentrifuged (Cytospin3, Shandon) at 163 g for 10 min onto Polysine slides (VWR). After mounting with Vectashield containing DAPI (Vector Laboratories) ...
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bioRxiv - Cancer Biology 2024Quote: ... We prepared E-media by mixing Dulbecco’s Modified Eagle Medium (DMEM) with Ham’s F12 3:1 and supplement with 10% Fetal bovine serum (FBS, from VWR), 0.5% Penicillin/Streptomycin Solution,10 µl of 25 µg/µl Hydrocortisone 16.8 µl of 0.25 µg/µl Cholera toxin ...
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bioRxiv - Bioengineering 2024Quote: ... The media collected from 3 flasks (25mL/T-175flask) was filtered through a 0.2μm filter (VWR, Cat#10040-464) followed by first round of ultracentrifugation (Thermo Scientific™ Sorvall™ WX ultraCentrifuges ...
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bioRxiv - Neuroscience 2020Quote: ... 5 Prime heavy 2 mL-phase lock gels (#2302830) were from VWR (Radnor, PA). High Capacity cDNA Reverse Transcription Kit (#4368814 ...
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bioRxiv - Microbiology 2023Quote: ... every 2 days or a cocktail of PGEs inhibitor (CAY10526 5 mg/kg VWR) and prostanoid receptor antagonist 2 (AH6809 5mg/kg Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Synthetic Biology 2022Quote: Single colonies were picked and cultured for 24 hours at 30 °C in 3 mL YPD liquid medium supplemented with appropriate antibiotics in 14 mL culture tubes (VWR) as seed culture ...
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bioRxiv - Bioengineering 2021Quote: ... formulations were aliquoted 150 μL per well (n = 3/group) in a clear 96-well plate and sealed with optically clear and thermally stable seal (VWR). The plate was incubated in a microplate reader (BioTek SynergyH1 microplate reader ...
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bioRxiv - Microbiology 2020Quote: ... and 3 μL of the culture was inoculated into 3 mL of MSgg liquid media in a 6-well or 12-well microtiter plate (VWR). The plates were incubated at 30°C for 2-3 days ...
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bioRxiv - Neuroscience 2020Quote: ... and then cryoprotected for 3 days at 4 °C in 30% sucrose solution (w/v in 1× PBS; VWR Chemicals). Brains were then embedded into optimal cutting temperature (OCT ...
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bioRxiv - Microbiology 2020Quote: ... or 0.4 µM (cesA) of each primer (Table 3) and 60 ng of DNA (measured by a MySpec spectrophotometer, VWR International). qPCR reaction conditions for the panC detection followed the master mix supplier’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with PBS 3 times and fixed and permeabilized with 3.7% formaldehyde and 0.3% Triton-X (VWR 80503-490) in PBS for 30 minutes at room temperature ...
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bioRxiv - Genetics 2022Quote: ... Pre-denaturation was performed at 91°C for 3 min and then 60°C for 20 min on heat blocks (VWR). After plates were spun ...
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bioRxiv - Genetics 2022Quote: ... Predenaturation was performed at 91 °C for 3 minutes and then 60 °C for 20 minutes on heat blocks (VWR). After plates were spun ...
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bioRxiv - Immunology 2020Quote: Wild-type (WT) and TLR4 knockout (KO) BMDMs were thawed and cultured for 3 days in non-Stissue culture treated dishes (VWR) in RPMI 1640 medium with L-Glutamine containing 10 % FBS and 10 % CM (= complete medium) ...
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bioRxiv - Physiology 2021Quote: ... 200 µl of these fixed RBCs were re-suspended in a blocking buffer containing 3% bovine serum albumin (VWR 0332) and 1% normal goat serum (Lampire Biological Laboratories 7332500 ...
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bioRxiv - Cancer Biology 2021Quote: ... ∼1 in wide and 3-4 in long into a 15 cm petri dish (CA25383-103, VWR, Edmonton, AB, Canada) and created spherical depressions ∼2.5-3 mm in diameter to create a mold for the Matrigel ...
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bioRxiv - Bioengineering 2022Quote: ... formulations were aliquoted 150 μL per well (n = 3/group) in a clear 96-well plate and sealed with optically clear and thermally stable seal (VWR). The plate was incubated in a microplate reader (BioTek SynergyH1 microplate reader ...
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bioRxiv - Genetics 2022Quote: ... A nonspecific antibody binding was prevented by an incubation of the fixed embryos in 3% BSA (VWR Life Science, 422351S) in PBS-T (1h ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed off the filter using 5 mL ice-cold methanol/ethanol/chloroform 1:3:1 (methanol: HiPerSolv Chromanorm, VWR Chemicals, Dresden, Germany; ethanol: HiPerSolv Chromanorm, VWR Chemicals ...
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bioRxiv - Developmental Biology 2023Quote: ... we aspirated ovarian follicles 3-8mm in diameter using an 18g needle (Single-Use Needles BD Medical, VWR, Philadelphia, PA) connected to a regulated vacuum system and collection bottle containing oocyte collection medium (OCM ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips coated with 0.1% gelatin were fixed for 20 minutes in 3% paraformaldehyde (VWR, Radnor, PA) (resuspended in PBS and brought to pH 8.0 with NaOH) ...
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bioRxiv - Molecular Biology 2023Quote: Monocytes were isolated as described above and 3×106 cells/ml were plated in a 6-well plate using RPMI-1640 medium (#392-0427, VWR) supplemented with 10% FBS (#11573397 ...
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bioRxiv - Cancer Biology 2023Quote: ... the upper part of the membrane was cleaned with cotton swab and lower part was stained with DAPI and the intensity of DAPI was measured using FlexStation 3 Multimode Plate Reader (VWR).
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bioRxiv - Bioengineering 2023Quote: ... 3% (w/v) alginate in in serum-free DMEM)and containing cells was placed in a Luerlock syringe (VWR, LOCA120006IM). 488mM calcium sulfate in serum-free DMEM was loaded into another Luerlock syringe and mixed with the cell-alginate solution through a Luerlock connector ...
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bioRxiv - Neuroscience 2024Quote: ... a 1-3 day old virgin female was placed in a 5.0 ml culture tube (VWR, Cat. No. 60818-565) in an ice bath for approximately 30 seconds until the fly was completely immobilized ...
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bioRxiv - Cell Biology 2024Quote: ... BLOC-1-deficient melan-mu melanocytes were plated at 3 x 104 cells/ml in 35 mm glass bottom dishes (VWR) in the presence or absence of 20 µM forskolin for 6 days ...
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bioRxiv - Bioengineering 2024Quote: ... CV was measured in 1 mM [Fe(CN)6]3- in 1 M KCl solution (VWR, catalog no. BDH9258-500G) at a scan rate of 200 mV/s.
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bioRxiv - Microbiology 2022Quote: ... 0.67% (w/v) Yeast Nitrogen Base without amino acids (VWR International), and a self-made 20-fold amino acid drop-out mix.55 Nourseothricin was used at 100 μg/mL final concentration in liquid or solid YPD media (yeast extract 2% ...
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bioRxiv - Immunology 2020Quote: RNA was extracted using a modified phenol-chloroform protocol (Chomczynski and Sacchi, 2006) with 1-Bromo-3-chloropropane and Isopropanol (Sigma and VWR, respectively). Total RNA was quantified and assessed for quality and integrity by Bioanalyser (RNA Pico 6000 Chip ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with TBS-T (20 mM Tris-HCl, pH 7.6, 136 mM NaCl, 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1h ...
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bioRxiv - Microbiology 2024Quote: ... the transgenic gametocytes were pelleted at 1,400 xg for 3 minutes and fixed in 4% v/v paraformaldehyde (VWR, Cat# PI28908) and 0.0075% v/v glutaraldehyde (VWR ...
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bioRxiv - Biochemistry 2024Quote: ... 40 mM imidazole and 200 mM NaCl was added before loading on a self-packed 3 ml Ni Sepharose™ 6 Fast Flow (VWR) column using ÄKTA Pure FPLC system (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... for 50 ms at 950 ms intervals using electrodes (diameter 3 mm; Sonidel CUY650P3) and ECM-830 BTX square wave electroporator (VWR international). After electroporation ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... whereas other enzymes were removed by passing the sample through a 3 kDa NanoSep centrifugal filter device (VWR, Radnor, PA, USA).
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bioRxiv - Developmental Biology 2023Quote: ... for 50 ms at 1 s intervals using electrodes (diameter 3 mm; Sonidel CUY650P3) and ECM-830 BTX square wave electroporator (VWR international). After electroporation ...
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bioRxiv - Microbiology 2024Quote: ... DBS samples were made by spotting 8 μL of sample onto Whatman grade 3 qualitative filter paper (VWR, cat #1003-917) cut to 96-well plate size ...
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bioRxiv - Cancer Biology 2024Quote: The SKOV-3-luc ovarian cancer cell line was harvested and washed with Dulbecco’s phosphate-buffered saline (DPBS, VWR, Oslo, Norway), and the cell number and viability were determined using a Countess Cell Counter (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... 0.4 g of poly(FATRIFE-ter-PEGMA-ter-NBMA) terpolymer was dissolved in 25ml methyl-ethyl ketone (25642.325, VWR, UK) in a 500 ml rotary evaporator flask containing 20 g of FEP at 40 °C to coat FEP particles ...
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bioRxiv - Microbiology 2023Quote: Each sample was chemically disrupted with an equal volume of 1:1 solution of ethyl acetate (EtOAc, VWR HiPerSolv Chromanorm) and methanol (MeOH ...
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bioRxiv - Microbiology 2024Quote: Each sample was chemically disrupted with an equal volume of 1:1 solution of ethyl acetate (EtOAc, VWR HiPerSolv Chromanorm) and methanol (MeOH ...
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bioRxiv - Cancer Biology 2024Quote: ... and 5 mL of 50 mM ammonium bicarbonate (ABC) + 2 M urea buffer (VWR, M123-1KG). After washing ...
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Synaptonemal Complex dimerization regulates chromosome alignment and crossover patterning in meiosisbioRxiv - Cell Biology 2020Quote: ... pSUN-1::TIR-1::mRuby him-8(tm611) spo-11-aid IV (ROG212) adult hermaphrodites were grown on 1 mM auxin (indole-3-acetic acid, VWR AAA10556-36) and therefore lacked SPO-11 ...