Labshake search
Citations for VWR :
201 - 235 of 235 citations for n1 Alpha L arabinopyranosylamino guanidine hno3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: Frozen tissue was briefly thawed and mechanically lysed by addition of 100 μL lysis buffer (5% SDS, 50 mM Tris, pH 7.55 with HCl) and 10 ‘strokes’ with a pestle (VWR International) after which the pestle was washed with 100 μL additional lysis buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... boiled at 95°C for 5 min in a closed vial and loaded on an 8% polyacrylamide gel containing 7 M urea for electrophoresis-based molecules separation at 300 V for 140 min using a gel transfer system (Doppel-Gelsystem Twin L, PerfectBlue-VWR).
-
bioRxiv - Bioengineering 2021Quote: ... Stained tissue sections were washed in 0.1% Triton-X/PBS at RT for 1 hour and mounted on a poly-L-lysine coated coverglass (VWR, 48393-241) for imaging (Fig ...
-
bioRxiv - Microbiology 2020Quote: ... Two-µl spots of each dilution were plated on selective (20 µg/mL thiamphenicol) and unselective square (90×15 mm, VWR international) BHI plates with or without 200 ng/mL anhydrotetracycline (ATc) ...
-
bioRxiv - Microbiology 2020Quote: ... we used 24.4 ± 1.7 mM fructose (146.4 ± 10.3 mmol C L−1) and the following carboxylates at a concentration of 108.2 ± 8.0 mmol C L−1: Na-acetate (VWR, Solon, OH, USA), Na-butyrate ...
-
bioRxiv - Genetics 2020Quote: ... the cells were resuspended in 1 ml of sterile water and spread on a plate containing minimal media lacking the corresponding amino acid or nucleotide (YNB or Synthetic Complete (SC): 6.7 g/l yeast nitrogen base (VWR 97064-162), 20 g/l glucose ...
-
bioRxiv - Neuroscience 2021Quote: ... mice were deeply anaesthetized with isoflurane (4% flow rate of 0.5–0.7 l/min) and transcardially perfused with cold phosphate-buffered saline (PBS) followed by 4% PFA (VWR International Inc, 15710). Brains were extracted and placed in PFA for 24 h and then transferred to 30% sucrose/PBS solution at 4 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... The 5 L bioreactors were grown for one to two weeks pl shredded (VWR 200 Homogenizer on high for one minute) and transferred to 12L bioreactors ...
-
bioRxiv - Immunology 2021Quote: ... 2mM l-glutamine and 25mM HEPES) were incubated for 96 hours at 37°C either on a 360° rotator (VWR International) or standing stationary (as specified) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... boulardii cultures were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
-
bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
-
bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
-
bioRxiv - Synthetic Biology 2022Quote: ... boulardii strains were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
-
bioRxiv - Microbiology 2024Quote: ... spun for 30min at 4°C 4,000g and protein purified from supernatant using glutathione agarose beads (Pierce, 16100) and elution with reduced L-Glutathione (VWR, 76177-906). Protein purity was confirmed on a protein gel and concentration was determined using a Nanodrop ...
-
bioRxiv - Microbiology 2024Quote: ... Phages were routinely titered by the soft agar overlay method(28) using TSA bottom plates and top agar consisting of 10 g/L Bacto™ tryptone (VWR), 10 g/L NaCl and 0.5% Bacto™ agar (VWR) ...
-
bioRxiv - Physiology 2024Quote: ... the cells were washed twice with 1x DPBS and stained with secondary antibodies: Alexa Fluor® 594 AffiniPure Donkey Anti-Rabbit IgG (H+L) (Cat #102649-732, 1:300, VWR), and Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG (H+L ...
-
bioRxiv - Bioengineering 2021Quote: ... A 15-µl aliquot of the master mix was combined with 10 µl of sample in a 96-well PCR plate (VWR, 83007-374). The plate was mixed on a Qiagen TissueLyser II for two 25-second intervals at 13 Hz then centrifuged for 2 minutes at 1000 xg.
-
bioRxiv - Microbiology 2022Quote: ... the mice were kept on standard drinking water or were provided water supplemented with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) for two days before the infectious blood meal to select for gametocytes ...
-
bioRxiv - Neuroscience 2023Quote: ... and BHK-B19L cells (see above) were plated in 10 cm plates coated in poly-L-lysine in normal medium (10% fetal bovine serum (VWR 16777-014) and antibiotic-antimycotic (Thermo 15240096 ...
-
bioRxiv - Cell Biology 2023Quote: ... The stained tissue sections were washed in 0.1% Triton-X/PBS at RT for 1 hour and finally mounted on poly-L-lysine coated cover glasses (VWR, 48393-241) for triple-view line confocal super-resolution imaging20.
-
bioRxiv - Microbiology 2024Quote: ... 200 µL of the bacteria were pipetted on 6 cm NGM plates and evenly spread across the agar plate using a sterile L spreader (VWR 490007-358). Bacterial plates were left to dry in a laminar flow hood for 2 days ...
-
bioRxiv - Microbiology 2024Quote: ... 1 mL of M9 was added to the plate and the bacterial lawns were scraped with a sterile L spreader (VWR 490007-358). Bacterial slurry was pipetted and centrifuged at 13000 x g for 4 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 21°C in shaking culture in HL5 medium (Formedium, Norfolk, England) and on SM/5 agar plates (2 g/L glucose (VWR, Solon, OH), 2 g/L bacto peptone (Becton ...
-
bioRxiv - Cancer Biology 2023Quote: ... NAC-containing water was prepared using reverse osmosis filtered tap water and granulated N-acetyl-L-cysteine (>99%, MilliporeSigma Canada, Oakville ON) and was sterilized using 0.2 um PES filter units (VWR Canada, Mississauga ON). The prepared water was stored for a maximum of 2 months before use ...
-
bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
-
bioRxiv - Bioengineering 2024Quote: ... the femur and tibia were extracted from the mice and placed in a wash media that consisted of DMEM/F-12 (1:1) with L- glutamine (VWR, Radnor, PA), 10% fetal bovine serum (Atlanta Biologics ...
-
bioRxiv - Bioengineering 2024Quote: ... The cell suspension was then centrifuged at 300g for 5 mins and re-suspended in DMEM/F-12 with L-glutamine (VWR, Radnor, PA), 10% fetal bovine serum ...
-
bioRxiv - Bioengineering 2024Quote: ... The cells were then centrifuged at 300 x Gs for 5 min and the pellet was resuspended in DMEM/F-12 with L-glutamine (VWR, Radnor, PA), 10% fetal bovine serum ...
-
bioRxiv - Cancer Biology 2024Quote: ... MITF Cat. # L-008674-00-0005) or Non-Targeting control (Dharmacon, Cat. # D-001810-10-05) using JetPrime (VWR, Cat. # 89129-924) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Blood was collected by cardiac puncture and then was maintained at 37°C in incomplete RPMI with 25mM HEPES and L-Glutamine (VWR Cat# 45000-412). Infected red blood cells were enriched by an Accudenz discontinuous gradient as previously described [30] ...
-
bioRxiv - Cancer Biology 2021Quote: 1×105 A549 or H460 cells in 200 µl of serum-free growth medium were seeded in the insert of a transwell plate with 8 μm pores (VWR International, Radnor, PA). These cells were coincubated with AvFc or CTX at 30 nM for 2 hours at 37 °C ...
-
bioRxiv - Immunology 2021Quote: ... and is composed of amphibian-adjusted Leibovitz’s L-15 (AL-15; Wisent, Mont-Saint-Hilaire, Canada) medium supplemented with 15% fetal bovine serum (FBS; lot # 234K18; VWR, Radnor, United States). Xela low serum medium is composed of AL-15 medium supplemented with 2% FBS ...
-
bioRxiv - Neuroscience 2020Quote: ... was mixed with deuterium oxide (450 µl) containing sodium formate (1 µmol/L) and then passed through a 10 KDa cutoff centrifugal filter (VWR, Radnor, PA, United States). 1H-NMR spectra were acquired using a 600 MHz spectrometer (Bruker AVANCE II ...
-
bioRxiv - Neuroscience 2023Quote: ... 2h,i,j,l,m,o) RNAscope was followed by standard immunofluorescent staining using rabbit-anti-RFP (Avantor VWR, RL600-401-379, 1:1000) as the primary antibody ...
-
bioRxiv - Plant Biology 2020Quote: ... using a digital scale (TC-204, Denver instruments, Arvada, CO, USA) and then moved into a forced-air oven (VWR Oven F Air 104 L, VWR, Radnor, PA) at 65 °C to dehydrate ...