Labshake search
Citations for VWR :
201 - 250 of 438 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... in 1X PBS at 4°C before embedding them in Optimal Cutting Temperature (OCT) Compound (VWR Chemicals) and snap freezing them with isopentane on dry ice ...
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bioRxiv - Neuroscience 2020Quote: ... and DNase I (20μg/ml) at 37°C for 75 minutes in an incubating mini shaker (VWR). The digested brain material was pelleted by centrifugation and resuspended in 20% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: All strains were grown on Czapek Dox Agar (CDA) at 37°C in an incubator (VWR International) in the dark over four days ...
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bioRxiv - Neuroscience 2022Quote: ... pH 8.4) in a 35 mm Mat-Tek glass coverslip bottom dish (VWR P35G-0-14-C) at an angle perpendicular to the liquid’s surface ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 48-hour incubation at 4°C in 50% optimal cutting temperature (OCT; VWR, #C361603E)/30% sucrose/phosphate buffer solution prior to freezing in 100% OCT using liquid nitrogen-cooled 2-methylbutane (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... size fractions were shaken overnight at 56 °C using the incubation chamber INCU Line ILS 6 (VWR). After the lysate cooled down ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with an antibody against RFP (1:1000, Rockland (VWR), catalog #RL600-401-379) ...
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bioRxiv - Cancer Biology 2022Quote: ... for 2 hours at 56°C and 50 μg of RNase A (VWR International, Radnor, PA, USA) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... counted and kept frozen in aliquots at −150°C in FBS with 10 % dimethyl sulfoxide (DMSO, VWR) for future experiments ...
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bioRxiv - Developmental Biology 2022Quote: ... This was followed by 3 x 1-hour incubations at 65°C with Histosec® (1.15161.2504, VWR). 5μm histological sections were cut using a Thermo HM325 microtome ...
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bioRxiv - Physiology 2022Quote: ... Samples were kept at -20°C throughout the extractions in an Iso-Therm System (VWR #20901-646). Fifteen milligrams of liver were homogenized in 200 µL of a 3:3:2 solution of acetonitrile:isopropanol:water (MeCN:IPA:H2O ...
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bioRxiv - Biophysics 2023Quote: ... were soaked for 1 hour at ~ 60°C in 2% v/v cleaning detergent (MICRO-90, VWR), thoroughly rinsed with 18.2 MΩ ultra-pure water ...
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bioRxiv - Bioengineering 2024Quote: ... The blood placed into a 0.5 ml Greiner Bio-1 minicollection tubes (VWR, International L. L. C.) with ethylene diamine tetra-acetic acid (EDTA ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a centrifugation (10 minutes, 16.100 x g, 4°C, MicroStar 17R centrifuge, VWR, Darmstadt, Germany) for sample clearance ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A vial of human primary nasal epithelial cells was thawed and aliquoted into multiple T25 cell culture flasks (VWR, UK). Cells were maintained in AECGM until confluent ...
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bioRxiv - Cell Biology 2021Quote: 3,000 ESCs/well mixed with 0.3 μg Wnt3a-beads/well were seeded onto human fibronectin (10 μg/mL, cat. um. 7340101, VWR/SLS) coated 8-well slides (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Human neuronal cells SH-SY5Y (ATCC, Manassas, VA, #CRL-2266) were cultured in DMEM medium (VWR, Radnor, PA, #0101-0500) supplemented with 10% FBS (Gemini Biosciences West Sacramento ...
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bioRxiv - Microbiology 2019Quote: ... strains [69], were maintained in human foreskin fibroblasts (ATCC; Manassas, VA) in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA) supplemented with 10% fetal bovine serum (VWR) ...
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bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were enriched by negative selection using StemCell RosetteSep Human Monocyte Enrichment Cocktail (StemCell 15068; 75μl per ml of blood) and Ficoll-Paque (VWR 17144003). Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034 ...
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bioRxiv - Microbiology 2023Quote: Toxoplasma strain TATi-RHΔku80 [41] was cultured in human foreskin fibroblasts in Dulbecco’s Modification of Eagle’s Medium (DMEM) (VWR; Radnor, PA, USA) supplemented with 10% fetal bovine serum (VWR) ...
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bioRxiv - Cell Biology 2019Quote: ... lot# 177772) and shaken at 37 °C overnight in a shaker at 1200 rpm (VWR Cat#12620-942).
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bioRxiv - Biophysics 2021Quote: ... was deposited on C-Flat holey carbon grids (hole size: 1.2 µm; spacing: 1.3 µm; mesh: 400, (VWR)) and a FEI Vitrobot Mark IV was used to plunge freeze the grids ...
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bioRxiv - Microbiology 2021Quote: ... were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 5% fetal bovine serum (VWR) and 100 µg/ml of penicillin-streptomycin (Wisent) ...
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bioRxiv - Microbiology 2020Quote: ... were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 5% fetal bovine serum (VWR) and 100 μg/ml of penicillin-streptomycin (Wisent) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... which we then incubated at 25°C for 4 days prior to staining with iodine (VWR iodine crystals) vapor (Forsburg and Rhind 2006).
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bioRxiv - Immunology 2021Quote: ... were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 5% fetal bovine serum (FBS) (VWR) and 100 μg/ml of penicillin-streptomycin (Wisent) ...
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bioRxiv - Microbiology 2020Quote: ... were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 5% fetal bovine serum (VWR) and 100 μg/ml of penicillin-streptomycin (Wisent) ...
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bioRxiv - Microbiology 2021Quote: ... were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 5% fetal bovine serum (VWR) and 100 μg/ml penicillin-streptomycin (Wisent) ...
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bioRxiv - Genomics 2022Quote: ... and salinity with a traceable digital portable conductivity meter (Accuracy: 0.3%, Temperature Range: –30.0 to 130.0°C, VWR, USA). In combination with the probe measurements ...
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bioRxiv - Bioengineering 2019Quote: ... Membranes were then heated for twelve minutes at 85°C in a table-top oven (VWR, Radnor, PA) and cut into strips with the laser cutter to create closed valve strips (Phillips et al. ...
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bioRxiv - Microbiology 2020Quote: ... were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 5% fetal bovine serum (VWR), 100 UI/ml of penicillin and 100μg/ml of streptomycin (Wisent) ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Cell Biology 2021Quote: ... incubated at 37°C for three mins, washed with Wash medium (DMEM, 0.1% BSA) + 5mg/ml DNase (VWR) and centrifuged at 300 rcf for three mins ...
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bioRxiv - Cell Biology 2021Quote: ... for two hours at 37°C and washed with Wash medium (DMEM, 0.1% BSA) + 1mg/ml DNase (VWR) twice and centrifuged at 300 rcf for three 3 mins ...
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bioRxiv - Microbiology 2019Quote: ... Tissue cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, (VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
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bioRxiv - Microbiology 2020Quote: ... were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Wisent) containing 5% fetal bovine serum (VWR), 100 UI/ml of penicillin and 100μg/ml of streptomycin (Wisent) ...
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bioRxiv - Developmental Biology 2021Quote: ... Fetuses and brains were then stored long-term at 4°C in 0.002% Sodium Azide (VWR - Bie&Berntsen) in PBS.
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bioRxiv - Microbiology 2022Quote: ... gondii co-cultures were maintained at 37°C with 5% CO2 in D10 medium (Dulbecco’s Modified Eagle Medium, 4.5 g/L glucose, VWR) supplemented with 10% Cosmic Calf serum (HycloneTM ...
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The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... and incubating islets at 37°C for ∼4 minutes with trituration occurring every 2 minutes (VWR, #CA10215-416). Accutase was neutralized with 600 µl RPMI 1640 + 10% FBS + 1% 10000 IU/ml penicillin-streptomycin ...
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bioRxiv - Molecular Biology 2023Quote: ... a 3 μl aliquot of the RHDV-80S solution was applied to grids (holey carbon grids C-flat 1.2/1.3 400 mesh VWR) that had undergone plasma cleaning for 6 seconds in a mixture of O2 and H2 by a Gatan Solarus 950 (Gatan ...
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bioRxiv - Microbiology 2024Quote: ... were added by incubating samples for 3.5 hours at 37°C in 3% BSA (VWR Cat# 97061-422) in PBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... sonicated at 60°C for 30 min using a USC100TH sonicator (VWR, Vienna, Austria, 60 W, 45 kHz), and stored at -20°C ...
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bioRxiv - Genomics 2020Quote: Single cell suspensions in PBS with 2% FBS were stained by incubating for 15 minutes at room temperature protected from light with anti-human PTPRC (CD45) monoclonal antibody conjugated to FITC (1:200 dilution, VWR #ABNOMAB12230), anti-human EPCAM antibody conjugated to PE (1:50 dilution ...
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bioRxiv - Neuroscience 2019Quote: Cryosections (20 – 40 μm) were generated using a cryostat (MICROM International, #HM560) on organoids and human retina embedded in OCT compound (VWR, #25608‒930). Sections were mounted onto Superfrost Plus slides (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Averaged dry weights were calculated in triplicate after heating samples at 100 °C for 48 h (VWR 1530 Incubator).
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bioRxiv - Cancer Biology 2021Quote: ... Cell supernatants were collected at different time points and centrifuged 30min at 10,000 × g at 4°C to eliminate cell debris (centrifuge CT15RE, VWR), then at 100,000 × g for 3h to eliminate larger vesicles (L7-55 Ultracentrifuge ...
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bioRxiv - Microbiology 2021Quote: ... we added 40 mg of powder yeast extract g−1 dry soil as a C and nutrient source (VWR). We chose yeast extract over glucose to provide more varied forms of C and thus stimulate more diverse microorganisms (Fierer et al. ...
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bioRxiv - Biochemistry 2022Quote: ... and were treated for 2hrs at 60°C with activated Pronase E (100µg/ml final concentration, pre-treated for 2hrs at 60°C in the digestion buffer; supplier: VWR). The sacculi were incubated at 100°C for 10min to inactivate the Pronase E and were washed 3 times with 1ml of MQ water ...
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bioRxiv - Immunology 2020Quote: ... Intestines were then cut into small pieces and incubated for 30 min at 37°C with agitation in a medium containing EDTA (2.5 mM, VWR) and DTT (72.5 µg/ml ...