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Citations for VWR :
201 - 250 of 1530 citations for 7 CHLORO 2H PYRIDO 2 3 B 1 4 OXAZIN 3 4H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Predenaturation was performed at 91 °C for 3 minutes and then 60 °C for 20 minutes on heat blocks (VWR). After plates were spun ...
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bioRxiv - Immunology 2020Quote: Wild-type (WT) and TLR4 knockout (KO) BMDMs were thawed and cultured for 3 days in non-Stissue culture treated dishes (VWR) in RPMI 1640 medium with L-Glutamine containing 10 % FBS and 10 % CM (= complete medium) ...
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bioRxiv - Physiology 2021Quote: ... 200 µl of these fixed RBCs were re-suspended in a blocking buffer containing 3% bovine serum albumin (VWR 0332) and 1% normal goat serum (Lampire Biological Laboratories 7332500 ...
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bioRxiv - Bioengineering 2022Quote: ... formulations were aliquoted 150 μL per well (n = 3/group) in a clear 96-well plate and sealed with optically clear and thermally stable seal (VWR). The plate was incubated in a microplate reader (BioTek SynergyH1 microplate reader ...
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bioRxiv - Genetics 2022Quote: ... A nonspecific antibody binding was prevented by an incubation of the fixed embryos in 3% BSA (VWR Life Science, 422351S) in PBS-T (1h ...
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bioRxiv - Developmental Biology 2023Quote: ... we aspirated ovarian follicles 3-8mm in diameter using an 18g needle (Single-Use Needles BD Medical, VWR, Philadelphia, PA) connected to a regulated vacuum system and collection bottle containing oocyte collection medium (OCM ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on glass coverslips coated with 0.1% gelatin were fixed for 20 minutes in 3% paraformaldehyde (VWR, Radnor, PA) (resuspended in PBS and brought to pH 8.0 with NaOH) ...
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bioRxiv - Molecular Biology 2023Quote: Monocytes were isolated as described above and 3×106 cells/ml were plated in a 6-well plate using RPMI-1640 medium (#392-0427, VWR) supplemented with 10% FBS (#11573397 ...
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bioRxiv - Cancer Biology 2023Quote: ... the upper part of the membrane was cleaned with cotton swab and lower part was stained with DAPI and the intensity of DAPI was measured using FlexStation 3 Multimode Plate Reader (VWR).
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bioRxiv - Bioengineering 2023Quote: ... 3% (w/v) alginate in in serum-free DMEM)and containing cells was placed in a Luerlock syringe (VWR, LOCA120006IM). 488mM calcium sulfate in serum-free DMEM was loaded into another Luerlock syringe and mixed with the cell-alginate solution through a Luerlock connector ...
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bioRxiv - Plant Biology 2021Quote: ... 7-day-old seedlings grown on hydroponic media were mounted on coverslips (24×50 mm No. 1½, VWR) using medical adhesive (Stock No ...
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... or xylene (VWR, Randor, VA, 1330-20-7) and rehydrated in a series of graded ethanol washes (100% ...
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bioRxiv - Neuroscience 2021Quote: ... 1:5000 dilution in PBS-T) for one hour and developed with chemiluminescent reaction (VWR, 490005-008).
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bioRxiv - Developmental Biology 2022Quote: ... one ventral EB and one dorsal EB were embedded together into the same Matrigel (Corning/VWR International, 354234) droplet in order to form a fused organoid ...
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bioRxiv - Bioengineering 2024Quote: ... OTMs on day 3 or day 7 post-inoculum (day 10/14 post-seeding) were punched into 7 mm discs using a biopsy punch (VWR, Philadelphia) to fit into a Transwell insert (#29442-082 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with TBS-T (20 mM Tris-HCl, pH 7.6, 136 mM NaCl, 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1h ...
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bioRxiv - Neuroscience 2019Quote: ... serum-free culture media containing secreted proteins were collected and concentrated in 0.5 mL 3-kDa molecular weight cut-off centrifugal filters (VWR North America). In some experiments ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was blocked with TBS-T (20mM Tris-HCl, pH 7.6, 136mM NaCl and 0.1% Tween-20) containing 3% BSA (VWR Life Science, VWRV0332) for 1 hour ...
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bioRxiv - Developmental Biology 2019Quote: ... 3 minutes), once with 2X SSC (room temperature, 3 minutes) and then treated with 50 ug/mL of RNase A (VWR 1247C393) in 2X SSC for 45 minutes at 37°C by overlaying the sample with 500ul of RNAse solution and incubating in a humidified chamber ...
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bioRxiv - Neuroscience 2023Quote: ... for 50 ms at 950 ms intervals using electrodes (diameter 3 mm; Sonidel CUY650P3) and ECM-830 BTX square wave electroporator (VWR international). After electroporation ...
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bioRxiv - Biochemistry 2023Quote: ... whereas other enzymes were removed by passing the sample through a 3 kDa NanoSep centrifugal filter device (VWR, Radnor, PA, USA).
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bioRxiv - Biochemistry 2024Quote: ... 40 mM imidazole and 200 mM NaCl was added before loading on a self-packed 3 ml Ni Sepharose™ 6 Fast Flow (VWR) column using ÄKTA Pure FPLC system (Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... in a rectangular one-well plate (VWR). The agarose was allowed to gel with the PDMS mold on top ...
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bioRxiv - Cancer Biology 2022Quote: ... Mobile phase B consisted of acetonitrile (VWR Chemicals) with 0.1% formic acid (v:v) ...
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bioRxiv - Plant Biology 2024Quote: ... and buffer B of 100 acetonitrile (VWR - 83640.320), The gradient elution was performed at a constant flow rate of 0.25 ml/min ...
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bioRxiv - Bioengineering 2021Quote: ... an aqueous solution of sodium dodecyl sulfate (SDS; concentrations: 2% or 4%; Cat. No. 97064-472, VWR) was instilled directly into the trachea via an inlet cannula ...
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bioRxiv - Cancer Biology 2023Quote: ... and 0.5% Triton X-100) on ice for 2 min and immediately fixed in formaldehyde 4% (VWR) for 10 min at the room temperature ...
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bioRxiv - Microbiology 2021Quote: ... air-dried and then extracted via gravity flow using 2 mL of methanol followed by 2 mL of methanol/tetrahydrofuran 1:1 (tetrahydrofuran HiPerSolv, VWR, Dresden, Germany). This extract was frozen until further chemical analysis.
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bioRxiv - Bioengineering 2022Quote: ... centrifuged for 3 minutes at 300 g and resuspended in E8 flex medium containing 10 μM Y27632 ROCK inhibitor (VWR, #688000-5). Cells were counted and plated at a density of 500 cells per well in a 96-well ultra-low attachment U-bottom plate (Corning ...
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bioRxiv - Biochemistry 2019Quote: ... E1A alternatively spliced isoforms were amplified from 3 μl cDNA by PCR in 50 μl reactions with the KAPA HiFi PCR Kit (Peqlab/VWR, Darmstadt, Germany) using primers E1A_ Exon1_forward 5’-GTT TTC TCC TCC GAG CCG CTC CGA-3’ and E1A_Exon2_reverse 5’-CTC AGG CTC AGG TTC AGA CAC AGG-3’ at final concentrations of 500 nM ...
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bioRxiv - Biochemistry 2020Quote: ... samples were washed for 3×5 minutes in PBSM and permeabilized for 10 minutes in PBSM + 5 mg/mL BSA (VWR VWRV0332-25G) + 0.1% Triton-X100 (Sigma-Aldrich T8787-100mL) ...
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bioRxiv - Cell Biology 2022Quote: ... Animals were then homogenized 20x in 3 mL of M9 buffer using an ice-cold 15 mL Dura-Grind Stainless Steel Dounce Tissue Grinder (VWR, 62400-686). Crude lysate was transferred to 1.5 mL tubes and frozen in liquid N2.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 2% FBS were injected into the left ventricle of the mice with a 26G × 3/8” needle attached to tuberculin syringe (VWR, Cat: BD309625). During the course of colonization assays ...
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bioRxiv - Molecular Biology 2021Quote: 2 x 107 BCBL-1 cells per time point were crosslinked with 2% formaldehyde (VWR M134) in prewarmed 1X phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then subcultured 1:1000 into 7 mL of MSgg in a 6-well polyvinyl plate (VWR, PA, USA). Plates were incubated in static conditions at 30°C for 3 days and were then imaged using a Leica MZ10F macroscope and Leica DMC2900 camera ...
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bioRxiv - Cell Biology 2023Quote: ... one D25 midbrain and one D25 forebrain organoid were placed in a deep well V-bottomed 96 well plate (VWR) with 2mL of COD+vitamin A media ...
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bioRxiv - Microbiology 2019Quote: ... Buffer B was 0.1% formic acid in acetonitrile (VWR). RP-HPLC conditions were as follows ...
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The aryl hydrocarbon receptor in β-cells mediates the effects of TCDD on glucose homeostasis in micebioRxiv - Molecular Biology 2023Quote: ... and incubating islets at 37°C for ∼4 minutes with trituration occurring every 2 minutes (VWR, #CA10215-416). Accutase was neutralized with 600 µl RPMI 1640 + 10% FBS + 1% 10000 IU/ml penicillin-streptomycin ...
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bioRxiv - Biochemistry 2021Quote: ... The 5 L bioreactors were grown for one to two weeks pl shredded (VWR 200 Homogenizer on high for one minute) and transferred to 12L bioreactors ...
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bioRxiv - Developmental Biology 2019Quote: ... Pancreas sections (7 μm thick) mounted on polysine slides (VWR) were de-waxed ...
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bioRxiv - Developmental Biology 2022Quote: ... 100ul of deionized Formamide (VWR Catalog # CAS-75-12-7) was added to 900ul of hybridization buffer (Stellaris ...
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bioRxiv - Plant Biology 2021Quote: ... and the seeds were removed with forceps and put in a drop of clearing solution (1 vol glycerol / 7 vol chloral hydrate liquid solution, VWR Chemicals) between a slide and a coverslip ...
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bioRxiv - Plant Biology 2023Quote: ... The seeds on their replum were then put on a slide with a drop of clearing solution (1 vol glycerol/7 vol chloral hydrate liquid solution, VWR Chemicals). The seeds were detached from the replum and the slide was covered with a coverslip and kept in the dark for at least 24h in a cold room (4°C) ...
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bioRxiv - Microbiology 2022Quote: ... 1-BuOH ANALAR and 2-propanol HPLC grade from VWR international GmbH ...
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bioRxiv - Molecular Biology 2021Quote: ... and the samples were vortexed 4 × 20 seconds at full speed on a vortex Genie 2 (VWR Scientific Products). The cellular debris were removed by centrifugation at 2,600g for 5 min at 4°C and 1.5 ml of the lysate was transferred to a micro-tube and centrifuged again at 9500 g for 5 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.01 mg/mL Hygromycin B (VWR, Cat# 45000-806). Cells were co-transfected with pXLone-dCas9-DNMT3A3L-P2A-EGFP and different pKH011-puro guide plasmids together with the piggyBAC transposase plasmid via electroporation with the Neon Transfection System (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... pH 7) plus 0.3 mg/liter methylene blue (VWR, Radnor, PA) to prevent microbial growth for the first 6 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Multiple 1 l cell cultures in 4 l flasks (VWR cat # 32645–044) were incubated 72 h at 27 °C ...
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bioRxiv - Plant Biology 2022Quote: ... 4-day-old seedlings were placed in a 1-well chambered coverglass (VWR, Kammerdeckglä ser ...