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Citations for VWR :
201 - 250 of 306 citations for 142mm Filter Holder Stainless Steel since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... a 500uL volume of each culture was filtered-sterilized (0.2 um centrifugal filter; nylon membrane; VWR 82031-358) and assayed in technical triplicate for enzymatic starch hydrolysis (see measuring amylolytic function below) ...
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bioRxiv - Cell Biology 2020Quote: ... 2900 rpm 10 min and 3500 20 min) and passed through a 0.22 µm PES membrane filter (VWR). To enrich EV concentration we used the Total Exosome Isolation kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... all proteins were diluted in filtered (sterile syringe filters 0.45 μm cellulose acetate membrane, VWR International, Radnor, USA) Min buffer in the presence of either 0.5 mM ADP or ATP ...
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bioRxiv - Genomics 2022Quote: ... Lentiviral-containing supernatants were harvested two days post-transfection and passed through a 0.45µm PES syringe filter (VWR). Ligand-expressing 293T cells were generated by transducing 293T cells (100,000 per well in a 12-well dish ...
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bioRxiv - Bioengineering 2022Quote: ... several milligrams of raw curcumin were dispersed in water and filtered using a 2 μm filter paper (VWR® Grade 410 ...
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bioRxiv - Microbiology 2023Quote: ... centrifuged for 15 min at 14,000 × g through 10 kDa molecular weight cut-off filters (modified PES, VWR), and transferred into HPLC vials.
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bioRxiv - Bioengineering 2024Quote: All the solutions used for the staining procedure were pre-filtered using 0.22 μm filter units (Milipore/VWR).
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bioRxiv - Cell Biology 2024Quote: ... Viruses were collected 48 hours after transfection and filtered through syringe filters with 0.45 µm pore size (VWR). Lentiviral transduction was conducted in 96-well plates at 30% cell confluency for 2 days and then cells were expanded to make stable expression lines and FACS sorted for polyclonal lines with a tight window for each fluorescent protein intensity.
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bioRxiv - Biochemistry 2024Quote: ... the sample was applied onto polyethersulfone membrane centrifugal filters with 3 kDa cutoff (VWR International GmbH, Darmstadt, Germany) to concentrate this fraction ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was collected 48 hours post-transfection and filtered through 0.45 μm cellulose acetate filters (VWR, 76479-040) or 0.45 μm PES filters (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... (fbB final concentration after filtration of a 6.0 mg/mL fbB solution with 0.45 μm PTFE filter (VWR), final volume 100 μL) ...
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bioRxiv - Cell Biology 2020Quote: ... virus-containing supernatant was collected 48 h later and filtered through a 0.45 μm polyethersulfone filter (VWR 28145-505). For protein-coding constructs ...
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bioRxiv - Microbiology 2021Quote: ... We mixed 30 μl of each lysate with 200 μl UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) in a 10 kDa MWCO 500 μl centrifugal filter unit (VWR International) and centrifuged the mixture at 14,000 x g for 40 min ...
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bioRxiv - Bioengineering 2021Quote: ... HI-FBS was then centrifuged at 100,000 x g for at least 16 h and the supernatant was filtered through a 0.20 μm bottle top filter (VWR) for subsequent use in BDMSC media.
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bioRxiv - Microbiology 2021Quote: ... followed by filtering the supernatant through a 0.45 μm pore membrane (sterile polyether sulfone filter, VWR North America, USA). Concentrated MS2 stocks were stored at 4°C for short durations (<2 days ...
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bioRxiv - Microbiology 2020Quote: ... and MD40HG cultures in 0.2% FLA-YM (as above) and a no cell negative control were filtered through 0.2 μm cellulose acetate membrane syringe filter (VWR). The filtrates were flash frozen and stored at −80°C until analysis ...
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bioRxiv - Systems Biology 2022Quote: ... we isolated the growth media from liquid cultures by flowing liquid cultures through a 0.2 µm pore filter (VWR). We ensured that no cells remained in the filtered media by flowing an aliquot through our flow cytometer ...
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bioRxiv - Synthetic Biology 2020Quote: ... centrifuged for 5 min at 4000 x g-1 and filtered over a 0.2 µm cellulose acetate filter (VWR). 1 ml aliquots were concentrated in a speed-vac (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: ... filtered through a Chromafil® GF/PET-20/25 filter (pore size 1.0 and 0.2 μm, VWR, Vienna, Austria) and stored at –20°C until analysis ...
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bioRxiv - Microbiology 2021Quote: ... Free biotin was removed by Macrosep Advance Spin Filter 3K MWCO 20 mL (Cat no. 89131-974, VWR, USA). Aliquots of the samples were stored at −80°C for use as input in western blot ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The phosphoproteome samples were resolubilized in 50 µL of 0.05% TFA in H2O/ACN 98/2 (v/v) filtered through 10 kDa MWCO PES membrane spin filters (VWR). The filtrate was also transferred to HPLC vials and injected into the LC-MS/MS instrument.
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bioRxiv - Plant Biology 2022Quote: ... The reference proteome sample was resolubilized in 30 µL of 0.05% TFA in H2O/ACN 98/2 (v/v) filtered through 10 kDa MWCO PES membrane spin filters (VWR). The filtrate was transferred to HPLC vials and injected into the LC-MS/MS instrument.
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bioRxiv - Biochemistry 2023Quote: ... 100 μL of slurry were added to a well of a 0.20 μm pore size 96 multi well filter plate (AcroPrep Advance, VWR). The beads were washed three times with 20% ethanol and 83% acetonitrile ...
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bioRxiv - Cancer Biology 2024Quote: ... culture medium was changed and lentivirus-containing supernatant was collected 48 hours after transfection and filtered through a 0.45 μm low-protein-binding filter (VWR). Target cells were plated in 6-well culture plates with a 60-80% confluency ...
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bioRxiv - Molecular Biology 2024Quote: ... we combined 60 uL of the lysate supernatant with 400 uL UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) in a 10 kDa MWCO 500 µl centrifugal filter unit (VWR International) and centrifuged for 40 minutes ...
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bioRxiv - Microbiology 2024Quote: ... with 400 μl of urea solution (8 M urea in 0.1 M Tris-HCl pH 8.5) and loaded it onto 10 kDa MWCO 500 μl centrifugal filters (VWR International) followed by centrifugation for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... with 400 μl of urea solution (8 M urea in 0.1 M Tris-HCl pH 8.5) and loaded it onto 10 kDa MWCO 500 μl centrifugal filters (VWR International) followed by centrifugation for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... lysate and UA solution were mixed in a 3:10 ratio (8 M urea in 0.1 M Tris/HCl pH 8.5) in a 10 kDa MWCO 500 µL centrifugal filter unit (VWR International) and the mixture was centrifuged at 14,000g for 40 min ...
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bioRxiv - Microbiology 2023Quote: ... and adding sterile filter double distilled water (liquid medium) or autoclaved molten 2% DNA pure agarose (VWR, Radnor, PA) (agarose plates ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Tryptic peptides were resolubilized in 30 μl of 0.05% TFA and 2% ACN in H2O filtered through 10 kDa MWCO PES membrane spin filters (VWR) at 14,000×g for 15 min and transferred to HPLC vials.
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bioRxiv - Microbiology 2023Quote: ... 60 µl of lysate was combined with 400 µl of UA solution (8 M urea in 0.1 M Tris/HCl pH 8.5) in 10 kDa MWCO 500 µl centrifugal filters (VWR International). Samples were centrifuged at 14,000 g for 30 min ...
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bioRxiv - Biophysics 2024Quote: ThT stocks were prepared by dissolving 1mM ThT in PBS and then sterile filtered through regenerated cellulose .22µm filters (VWR). Monomeric α-Synuclein was prepared by resuspending lyophilized protein in PBS buffer (Gen Clone (- ...
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bioRxiv - Microbiology 2024Quote: ... 2 ml of the culture was collected through centrifugation (5 min, 8000 rpm) and the supernatant filtered (0.22-μm filter; VWR). Secreted proteins in the supernatant were then precipitated using 10% ice cold trichloroacetic acid (TCA ...
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bioRxiv - Bioengineering 2024Quote: ... The media collected from 3 flasks (25mL/T-175flask) was filtered through a 0.2μm filter (VWR, Cat#10040-464) followed by first round of ultracentrifugation (Thermo Scientific™ Sorvall™ WX ultraCentrifuges ...
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bioRxiv - Immunology 2024Quote: Adhesion and migration of leukocytes through collagens was assessed using 24 well 3.0 µm pore transwell filters (29442-088, VWR) as previously described47 ...
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bioRxiv - Biophysics 2024Quote: ... All electrolyte solutions used in bilayer experiments were buffered with 10 mM phosphate and filtered through 0.22 µm pore size PES vacuum filters from VWR International (Radnor ...
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bioRxiv - Bioengineering 2024Quote: ... The harvested virus-containing DMEM medium was combined and centrifuged for 5 min at 1,500 rpm (Multifuge 3s, Heraeus) after which the supernatant was filtered using a 0.45 µm filter (514-0074, VWR) to remove residual producer cells and debris ...
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bioRxiv - Genetics 2024Quote: ... Viral supernatants were harvested 48-60 hours after transfection and filtered through a 0.45 μm PES syringe filter (VWR). CLTA-GFP HEK293T cells were transduced with lentiviral particles and subsequently sorted on mCherry expression to obtain a >99% sgRNA expressing cell line.
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were centrifuged at 14000 rpm at 4°C and the supernatants clarified with a 0.2 μm membrane syringe filter (VWR International) before proceeding with a two-step purification protocol ...
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bioRxiv - Plant Biology 2021Quote: ... about 200-300 EAs were transferred to a single layer of autoclaved sterile filter paper (Cat No. 28320-020, VWR) in 25 × 100 mm petri dish ...
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bioRxiv - Plant Biology 2021Quote: ... was kept at 30°C during the injections and all eluents were filtered through 0.2 μm PES filter (VWR, USA) and stored under helium ...
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bioRxiv - Microbiology 2020Quote: ... Cell debris and unbroken cells were removed by centrifugation and supernatants were passed through a 0.22 μm filter unit (VWR International). Soluble protein suspension was loaded onto the Bio-Scale Mini Profinity IMAC Cartridge (5 mL ...
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bioRxiv - Microbiology 2020Quote: Calibration of the device was carried out by spraying deionized water on 9 cm diameter filter paper (Qualitative 415, VWR). In order to understand the relationship between pressure ...
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bioRxiv - Genomics 2022Quote: ... The supernatant (which contains the majority of virus) was filtered through a 0.2 μM PES filter (VWR, cat. #97066-214). 25 mL of 40% PEG8000 (JT Baker ...
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bioRxiv - Microbiology 2021Quote: ... was added to each well of a 96-well AcroPrep filter plate with 0·2 μM wwPTFE membrane (514-1096, VWR). A100 μL of plasma sample ...
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bioRxiv - Biophysics 2020Quote: ... the untreated and treated bacteria (expressing H-NS-mEos3.2 fusion proteins) were filtered by 0.2 μm filters (VWR International LLC), resulting in filtrates constituting leaked cellular contents due to membrane damage ...