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Citations for VWR :
1651 - 1700 of 1771 citations for 8 chloro 10 3 dimethylamino propyl phenothiazin 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... 1 mL 5% PVA (w/v) in 200 mM sucrose was incubated for 1 h at 90°C in a thermal shaker lite (VWR international GmbH ...
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bioRxiv - Immunology 2020Quote: Cells of human or murine origin (0.2-1×106) were lysed in Trizol (peqGOLD TriFast™; VWR International, Radnor, USA) and total RNA was extracted by use of the DirectZol® RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: B16-F1 cells were transfected with 0.5 µg EGFP-β-actin and 1 µL JetPrime transfection reagent (VWR, #114-07) per well in 6-well plates according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed in RPMI before resuspension in buffer (0.2% FBS in PBS) at concentration of 1 × 106 cells/ml in FACS tubes (Falcon® Brand, VWR). Before addition of the cocktail of 21 monoclonal antibodies ...
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bioRxiv - Microbiology 2022Quote: ... The in-house polyacrylamide TBE gels (40 ml) for horizontal electrophoresis were produced by combining 6 ml 40% Acryl/Bis 29:1 (VWR), 4 ml of 5x TBE ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated overnight at 23°C in HL-5 medium supplemented with 200 µM sodium palmitate ...
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bioRxiv - Neuroscience 2022Quote: The coverslip seeded with the transfected cells was placed in a custom glass-bottom chamber based on Chamlide EC (Live Cell Instrument) with glass bottom made with a 24 × 24 mm #1 coverslip (89082-270, VWR). Cells were continuously perfused with the external solution at ∼ 4 mL/min with a peristaltic pump (505DU ...
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bioRxiv - Neuroscience 2023Quote: ... washed once in ice-cold 1× PBS with Ca2+/Mg2+ and then fixed in 4% paraformaldehyde (PFA, VWR: 100504-940) for 30 mins at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Microbiology 2024Quote: ... Then it was used to seed a main culture (200 µl final volume of BMM with desired concentration of Ca2+ and/or Fe) at a ratio of 1:100 in 96 well white clear bottom plates (VWR). The plates were covered with lids treated with a mixture of 95% ethanol and 1% Triton solution to prevent condensation ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Cell Biology 2024Quote: ... The remainder ground tissue was boiled for 2.5 h at 100 °C before being neutralized with 5 mol.L-1 KOH (VWR, United States). Total glucose (free glucose + glucose produced by glycogen hydrolysis ...
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bioRxiv - Bioengineering 2024Quote: ... Oral rinses in the anatomical scaffolds were performed on day 3 and day 5 post-inoculation using Listerine® Antiseptic mouthwash original (brown color) diluted in PBS (1x) to 1% and filtered using a vacuum filtration system (0.2 µm) (VWR, Philadelphia). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... 1:1000 LIF) and were pre-plated for 1-2 h to remove fibroblasts before they are seeded on matrigel (VWR) coated dishes ...
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bioRxiv - Microbiology 2023Quote: Cell extracts were dried in a nitrogen stream at room temperature and resuspended in either 200 µL methanol for diatom extracts or methanol/acetonitrile/water 5:9:1 (acetonitrile: HiPerSolv Chromanorm, VWR Chemicals ...
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bioRxiv - Genomics 2023Quote: ... 5000 cfu were plated in 1 mL media in each of 21 wells of a 24-well culture plate (10861-558, VWR), and initial cultures were plated for cfu on 7H10 + OADC to confirm number of cells in initial culture ...
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bioRxiv - Microbiology 2023Quote: ... The lentivirus-containing medium was then removed and cells were allowed to recover for 72 hours whereupon cells were passaged in the presence of 1 μg/mL puromycin (VWR) and remained under selection for 3 days.
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bioRxiv - Developmental Biology 2023Quote: ... Egg chambers were dissected and manually dechorionated in isolation buffer and then incubated for 30 min in Zalokar’s buffer containing 4.5% hydrogen peroxide and 1 mg/ml horseradish peroxidase (VWR, Radnor, PA) before bleaching as above.
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bioRxiv - Molecular Biology 2023Quote: ... Tissue sections were rinsed of excess probes in 2X SSCT for 1 min and washed twice in 50% formamide (VWR) in 2X SSC at 37°C for 25 min ...
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bioRxiv - Microbiology 2023Quote: ... in PBS pads were placed on the sample and were covered with 20×20 mm No 1 cover glass slide (VWR). The open sides between two cover glass slides were sealed using VALAP solution (1:1:1 w/w Vaseline-Lanolin-Paraffin).
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bioRxiv - Microbiology 2023Quote: ... PBS was added to wells (1 mL) and the base and sides of each well were scraped twice using a cell scraper (VWR) to disrupt biofilm ...
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bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 amoebae were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated at 23°C in HL-5 medium ...
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bioRxiv - Immunology 2023Quote: MDCK.2 cells (ATCC) were seeded at a density of 1 × 106 cells/well in 6-well plates (VWR International) and incubated overnight at 37℃ 5% CO2 ...
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bioRxiv - Plant Biology 2023Quote: ... stained with 1% w/v toluidine blue in 0.5% w/v sodium tetraborate for 5 seconds and mounted in DePeX (VWR, Belgium). For vibratome sectioning ...
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bioRxiv - Biophysics 2023Quote: ... at a mass ratio of 1000:1 TO:PL and bath sonicated for 30 min (Ultrasonic Cleaner 96043-936; VWR Symphony) to form aLDs ...
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bioRxiv - Developmental Biology 2023Quote: ... and then to a 52 mm x 76 mm x 1 mm (length x width x thickness) glass slide (VWR) in a low-pressure oxygen plasma generator (Femto ...
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bioRxiv - Developmental Biology 2023Quote: ... Bulk samples including bone and bone marrow were cryo-pulverized and resuspended in 1 ml ice-cold TriFast (VWR International) and carefully vortexed (30 sec) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1×104 cells in 100μL media were plated in the top of the Corning filter membrane insert (VWR, Radnor, PA) sitting inside of a 24 well tissue culture plate ...
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bioRxiv - Bioengineering 2023Quote: The strains were grown overnight in M9 minimal media (4 g/L glucose, 1 g/L casamino acids (cat. no 2240, VWR), and 1 mg/L thiamine (cat ...
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bioRxiv - Synthetic Biology 2023Quote: Cells carrying the open-loop and closed-loop versions of the composition controller circuits were grown as monocultures in 1 mL of rich M9 medium supplemented with the appropriate antibiotics in 2 mL 96-well deep-well plates (VWR) at 30°C in a plate shaker incubator (Infors HT Multitron ...
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bioRxiv - Bioengineering 2023Quote: ... The product was purified by a dialysis bag with 1 K MWCO followed by filtration with VWR syringe filter (0.22 μm; 76479-010; VWR, PA). To load DOX to GQDs (e.g. ...
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bioRxiv - Microbiology 2023Quote: Samples were prepared by drying fixed bacterial cells spotted onto poly-L-lysine coated coverslips (VistaVision cover glasses, No. 1; VWR) in a 4°C refrigerator and subsequent dipping into water three times to dissolve precipitates from the growth media ...
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bioRxiv - Immunology 2024Quote: ... The fixed cells were washed twice with cold flow staining buffer and blocked by 1% bovine serum albumin (BSA) (Fraction V, cat. 422371X, VWR) in PBS for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... The thin polyacrylamide-based substrate was polymerized between two different glass coverslips (#631-0162, diameter 32 millimeters, thickness No. 1, VWR) prepared as follows ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All evolution was conducted at 30°C in 128μL of YPD (a rich laboratory media; 1% Bacto yeast extract (VWR #90000– 722), 2% Bacto peptone (VWR #90000–368) ...
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bioRxiv - Molecular Biology 2021Quote: We prepared Aβ1-42 oligomers (oAβ) and fibrils (fAβ) following Evans (2006) and Ryan et al (2010) using siliconized polypropylene tubes (VWR #4165SL). We used Aβ1-42 to test the effects of the hybrid protein because it is more predominant in AD plaques (Nirmalraj et al. ...
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bioRxiv - Bioengineering 2021Quote: ... Stained tissue sections were washed in 0.1% Triton-X/PBS at RT for 1 hour and mounted on a poly-L-lysine coated coverglass (VWR, 48393-241) for imaging (Fig ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then normalized to 1 ng μL-1 or 2 ng μL-1 for genomic DNA extracted from fecal and cecal samples by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot ...
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bioRxiv - Neuroscience 2020Quote: ... Individual CNSs were sonified with five 0.3 s pulses at 1 Hz using a Branson Sonifier 450 (VWR Scientific, Winchester, PA) in Laemmli buffer (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Both filtered and unfiltered water samples were vortexed and diluted 1:5.75 into 2% (vol/wt) trace-grade nitric acid (VWR, Radnor, PA) in acid-washed polypropylene tubes and stored overnight at ambient temperature ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Coating was performed for 1 h with 15 μg/ml of fibronectin or with 3.5 μg/cm2 Cell-Tak (VWR, 734-1081) diluted in 0.1 M sodium bicarbonate ...
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bioRxiv - Genetics 2022Quote: ... The slide was then transferred to 37°C for 1 min before being immersed and fixated in methanol (VWR EU, 20847.307) at -20°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... The 12 lineages were evolved in 1 mL of antibiotic-free LB medium using 2 mL-deep-96-well plates in checkered pattern (VWR International) and incubated at 37°C with 700 rpm constant shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Plant Biology 2021Quote: ... and the seeds were removed with forceps and put in a drop of clearing solution (1 vol glycerol / 7 vol chloral hydrate liquid solution, VWR Chemicals) between a slide and a coverslip ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...
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bioRxiv - Bioengineering 2022Quote: ... containing 20% FBS (Wisent, 80450, lot 115714), 1% Penicillin-Streptomycin solution (Wisent, 450-201-EL) and 2,5 ng/mL bFGF (VWR, 10821-962) in a 37°C ...
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bioRxiv - Immunology 2020Quote: ... the monocytes were harvested and the pellet resuspended in differentiation media (RPMI-1640 supplemented with 1% FBS H.I., Penicillin/Streptomycin (P/S, VWR, Radnor, PA) and 100 ng/mL phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Microbiology 2019Quote: ... Growth in the phylloplane was assayed by re-isolating Protomyces from inoculated leaves by placing leaves in 2 ml Eppendorf tubes with 1 ml 0.025% Silwet-L77 in water and shaking (800 rpm; VWR microplate shaker) for 1 hour ...
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bioRxiv - Genomics 2020Quote: Single cell suspensions in PBS with 2% FBS were stained by incubating for 15 minutes at room temperature protected from light with anti-human PTPRC (CD45) monoclonal antibody conjugated to FITC (1:200 dilution, VWR #ABNOMAB12230), anti-human EPCAM antibody conjugated to PE (1:50 dilution ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were diluted in PBS with 2% FBS and samples were stored in PBS containing 1% formaldehyde (VWR Life Science AMRESCO). For intracellular staining ...