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Citations for VWR :
1551 - 1600 of 1623 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... and 1%(v/v) ammonium persulfate (APS) for 60 minutes at RT on plasma cleaned glass cover slips (No.1, 25mm Ø, VWR) that were pre-treated with 3-aminoproyltriethoxysiliane (APTES ...
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bioRxiv - Genetics 2023Quote: ... hTERT RPE-1 cells (ATCC, CRL-4000) were maintained in DMEM:F-12 media (ATCC, 30-2006) supplemented with 10% FBS (VWR, 97068-091) and 1% Pen-Strep (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 100 mg of minceate was then suspended in 1 mL ice-cold 0.3 M sucrose (Fisher, cat# BP220) in H2O supplemented with protease inhibitor cocktail (VWR, cat# M250) at 1:100 (1:10 w/v suspension) ...
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bioRxiv - Microbiology 2024Quote: ... aureus strains were generated as previously described 101 and transformed with ∼5 µg of the pIMAY construct using the BioRad Gene Pulser Xcell electroporation system and 1 mm disposable electroporation cuvettes (VWR Signature). The pulse was 21kV/cm ...
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bioRxiv - Physiology 2024Quote: ... the cells were washed twice with 1x DPBS and stained with secondary antibodies: Alexa Fluor® 594 AffiniPure Donkey Anti-Rabbit IgG (H+L) (Cat #102649-732, 1:300, VWR), and Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG (H+L ...
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bioRxiv - Physiology 2024Quote: ... permeabilized with 3% Triton X 100 solution for 15min (Carl Roth, Germany) and blocked with 5% Goat Serum (BioWest, France) and 1% Bovine serum (VWR, USA) for 1 h ...
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bioRxiv - Genomics 2024Quote: ... 100 uL HoxB8 neutrophils (1 × 106 cells/mL) were seeded into an 8-well Nunc Lab-Tek II Chamber Slide (VWR international) coated with 2% poly-L-lysine (MilliporeSigma) ...
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bioRxiv - Systems Biology 2024Quote: ... All antibodies were diluted at 1:100 in FACS buffer [PBS with 10% fetal bovine serum (FBS, VWR, MDTC35-016-CV) containing 1% Penicillin-Streptomycin solution (P/S ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells from four 80% confluent 10 cm Petri dishes were transfected with 0.9μg VSV-G envelope expressing plasmid pMD2 and 9 μg psPAX2 packaging vectors and 9μg of the gRNA library DNA in the presence of 113.4μL Polyethylenimine - PEI (VWR International, 1 mg/mL) per plate ...
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bioRxiv - Cell Biology 2024Quote: ... followed by resuspension in culture medium (DMEM low glucose, 10% HI-FBS, 1% Penicillin/Streptomycin) and filtered through a 40 μm cell strainer (VWR, USA). The obtained single cell suspensions of ovarian primary cells were seeded either for monolayer 2D culture (1.5 × 106 cells/well ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and was subsequently propagated in a well of an unshaken flat-bottom polypropylene 96-well plate in one of three conditions: YPD (1% Bacto yeast extract (VWR #90000–726), 2% Bacto peptone (VWR #90000–368) ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... we plated a 150 μL of 1:100 diluted saturated culture of a sensitive strain (YAN563) on a single-well (VWR #46600-638) methylene blue agar plate (20 g/L peptone ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated with chemiluminesence substrate (Perkin-Elmer: NEL105001EA) for 1 min and signal was developed on GE Amersham Hyperfilm ECL (VWR: 95017-661). Membranes were stripped by 2 x 7 min incubations in stripping buffer (6 M guanidine hydrochloride (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... The films were rinsed in dH2O and covered with large coverslips (80 × 120 mm, thickness no.1, VWR International AS, Oslo, Norway). Imaging was performed with an epi-fluorescence microscope (Olympus BX51) ...
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bioRxiv - Biophysics 2022Quote: ... the surface of the glass coverslip serving as chamber bottom was incubated with 1% (10 mg/ml) bovine serum albumin (BSA; VWR, Radnor, PA) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... each well was washed three times with warm PBS and then treated with 500ul PBS with 1% Triton X-100 (AMRESCO, VWR, Randor PA) for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck, Germany). For this purpose ...
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bioRxiv - Microbiology 2021Quote: ... enterocolitica samples were eluted by washing the wells 3 times with warm PBS followed by treatment with 500 μl PBS with 1% Triton X-100 (AMRESCO, VWR, Randor, PA) for 5 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... and 1/5th of the lysate was added into 40 µL lysis buffer and sheared in a sonicator (VWR Aquasonic 250D, 35Khz) for 3 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was changed and the incubation continued for another 24 hours before addition of 1-5 µg/mL puromycin (VWR 97064-280). Incubation with puromycin continued for several days until untransfected control wells were depleted of cells ...
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bioRxiv - Neuroscience 2021Quote: ... After which cells were washed once with 1x PBS with 1% BSA before being filtered through 40um FlowMi Tip Strainers (VWR, 10032-802). Cells were then diluted with 1x PBS with 1% BSA and loaded onto the Chromium 10x Controller according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of each reaction were mixed with loading dye and analyzed on a 1% (w/v) agarose gel containing GelRed (VWR, Darmstadt, Germany). The remaining PCR mix was purified using a QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... the tissue was first blocked for 30 min using a blocking buffer (TBS with 1% BSA (VWR, 22013, Bovine Serum Albumin (BSA), fraction V ...
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bioRxiv - Immunology 2021Quote: ... Whole blood cell lysates or lysates of PBMCs from IL-1-Ra-Ab positive patients were treated with alkaline phosphatase as previously described using FastAP thermosensitive alkaline phosphatase (Fermentas/VWR, Darmstadt, Germany).(40 ...
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bioRxiv - Bioengineering 2020Quote: ... and particles were removed from PDMS chip and glass substrate (76 x 26 x 1 mm microscope slides, VWR International GmbH, Germany) via isopropanol washing and drying with pressurized air ...
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bioRxiv - Cancer Biology 2021Quote: ... was achieved using an adapted protocol from Ly et al.22 FFPE TMAs were first washed twice for 1 minute in a xylene bath (mixture of isomers; ≥98.5%, AnalaR NORMAPUR®, VWR, Leicestershire, UK). Residual xylene was removed ...
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bioRxiv - Immunology 2020Quote: One mL of BMDM from lipin-1mKO and littermate control mice at a concentration of 1×106/mL was placed into falcon tubes (VWR-60818-500). Cells were either treated with or without IL-4 (40ng/mL ...
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bioRxiv - Immunology 2020Quote: ... Unbound protein was washed away with PBST (1x PBS with 0.5% Tween) followed by blocking with 1% casein (w/v) in PBS (VWR International, Radnor, PA) for 2 h at room temperature with mild shaking (55 rpm) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation) for 1 min at RT in a 1.5 mL centrifuge tube using a cordless motor (VWR Catalog # 47747-370) and pestle (USA Scientific Catalog # 1415-5390) ...
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bioRxiv - Plant Biology 2023Quote: ... from accessions grown in UC Davis or UC Riverside greenhouses and floated overnight in 1 mL deionized water in a 24-well tissue culture plate (VWR #10062-896) with a plastic lid to prevent evaporation of the water ...
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bioRxiv - Bioengineering 2023Quote: ... the strips were immersed in a blocking buffer (1% BSA and 0.05% Tween 20) for one hour on an orbital shaker (no. 57018-754VWR; VWR International, Brisbane, CA) set at 150 rpm and thereafter dried overnight at 4° Celsius ...
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bioRxiv - Cancer Biology 2023Quote: ... Each well is washed with 1 mL of phosphate buffered saline (PBS) prior to fixation with 500 µL of 10% buffered formalin (VWR, 89379-094). After at least 24 hours of fixation ...
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bioRxiv - Cell Biology 2023Quote: ... flies were then transferred to new vials containing a 1cm by 5cm strip of filter paper saturated with 300µl of 30% H2O2 (VWR, Cat# BDH7690-1) and placed at 25°C for 24 hours ...
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bioRxiv - Bioengineering 2024Quote: ... AB_11125142) or an anti-mouse secondary antibody (1:3000, Cat# 170-6516, RRID: AB_11125547) and enhanced chemiluminescence reagent (VWR, Cat # 490005-018). The signal was visualized using a C-DiGit Blot Scanner (Li-Cor) ...
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bioRxiv - Cell Biology 2023Quote: ... washed cells were suspended at a concentration of 1 × 106 cells/mL in the growth medium and filtered using a 40 µm cell strainer (VWR: 10199- 655). Cells were then sorted into 15 mL collection tubes using a sterile 100 µm chip (Sony ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were seeded at 1×106 cells/well in 24-well plates and maintained in X-VIVO-15™ media (CA12001-988, VWR, USA). Four treatment groups were used ...
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bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Cell Biology 2024Quote: ... the coverslips were PEGylated by incubating in a 1:100 mixture of 1% biotin-PEG-silane in ethanol (Laysan Bio Biotin-PEG-SIL-2K-1g) and PEG-silane (85%, VWR 77035-498) for one hour in the dark at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... pre-cultures for reporter-based promoter activity assays were performed at 30°C and 900 rpm in 1 ml medium in 2.2 ml square-shaped V-bottom 96-deep well plates (VWR, Radnor, US-PA) covered with a sterile breathable rayon film (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 mM Tris-HCl, pH 7.2, 5 mM EGTA, 100 mM KCl, 1% Triton-X100; all chemicals from VWR; Radnor, PA, USA). Samples were homogenized using tight-fitting pestles and centrifuged at 3,000 g for 30 minutes at 4ºC ...
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bioRxiv - Molecular Biology 2024Quote: ... The resin was equilibrated with wash buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM DTT [VWR Chemicals]) and bound proteins were eluted in wash buffer supplemented with 20 mM glutathione ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was lysed using lysis buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM dithiotreitol (DTT; [VWR Chemicals])) supplemented with protease inhibitor [cOmplete ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was lysed using lysis buffer (50 mM Tris-HCl [Sigma-Aldrich], pH 7.5, 250 mM NaCl [Sigma-Aldrich], 1 mM DTT [VWR Chemicals]) supplemented with protease inhibitor [cOmplete ...
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bioRxiv - Developmental Biology 2024Quote: ... was conducted in 1x Towbin buffer with 0.1 % SDS for 30 minutes (25 mM Tris-base, 192 mM Glycine, 1 % SDS) using a PerfectBlue Semi-Dry Electroblotter Sedec M (VWR; 700-1220). Transfer was performed at a constant current of 10 mA for 90 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... and the wing was imaged at selected ages through a cover glass of thickness 1.5 (VWR Micro Cover Glass, selected 1 ounce, 18×18 mm, No. 1.5, VWR Cat No 48366-205).
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bioRxiv - Cell Biology 2020Quote: ... Solutions were mixed by shaking the compound plate for 1 min at room temperature at 1200 rpm using a microplate shaker (cat. 12620-926, VWR, West Chester, PA). A typical plate layout map used for screening experiments is shown in Figure 1 ...
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bioRxiv - Biophysics 2020Quote: ... The sheath and sample fluid syringes (BD Luer-Lok™ 5-ml syringe #613-2043P and BD Luer-Lok™ 1-ml syringe #613-4971; VWR, Germany) were connected to the fabricated chip via FEP Tubing (1/16” OD ...
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bioRxiv - Neuroscience 2021Quote: ... and 4% TCA (100μl) were added to these tubes and vortexed for 1 minute on a multi-tube vortex mixer (VWR International LLC, Radnor, PA).
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bioRxiv - Neuroscience 2022Quote: ... mEPSCs were recorded at a holding potential of −70 mV in the presence of 50 μM PTX and 1 μM tetrodotoxin (TTX; VWR, Catalog#89160– 628). Electrophysiological data were analyzed using Clampfit 10.5 (Molecular Devices ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were mixed with the scintillation liquid and counted in a scintillation counter (Packard TriCarb Model, 1 min/vial) using Solvent-Saver™ scintillation vials (VWR International LLC.).