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Citations for VWR :
101 - 128 of 128 citations for Prion Protein Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The protein fraction was isolated by combining four times the volume of ice cold acetone (VWR Chemicals, PA, USA) to one volume of reconstituted PBMCsec ...
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bioRxiv - Systems Biology 2020Quote: ... Protein and crosslink digestion was carried out by adding 50 μL freshly prepared 20 mg/mL Proteinase K (VWR, freeze-dried powder suspended in 10 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins production was induced with 1 mM [F1] IPTG 3h [F2] treatment (Isopropil-β-D-tiogalattopiranoside, VWR, Pennsylvania, USA). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... cultured in 150mm dish (Alkali scientific TDN0150) for protein experiment and in 6-well culture plate (VWR, 100062-892) for total RNA experiment and transfected at around 50-60% confluency ...
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bioRxiv - Bioengineering 2023Quote: ... stably expressing enhanced green fluorescent protein (eGFP) were generated as described before[45] and cultured on 0.1% gelatin (VWR) pre-coated surfaces in MCDB-131 medium (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... culture medium was changed and lentivirus-containing supernatant was collected 48 hours after transfection and filtered through a 0.45 μm low-protein-binding filter (VWR). Target cells were plated in 6-well culture plates with a 60-80% confluency ...
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bioRxiv - Cell Biology 2019Quote: ... The donkey anti-rabbit (NA934, 1:1000) and anti-mouse (NA931, 1:1000) horseradish peroxidase (HRP)-linked secondary antibodies were purchased from VWR. The GFER rabbit polyclonal antibody (#HPA041227 ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibodies (1:1000 goat anti-GFP from Novus Biologicals or 1:10 000 rabbit anti-RFP from VWR (Rockland) were incubated with PGT overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Systems Biology 2020Quote: ... 4°C) and the soluble fraction was collected and protein precipitated using ice-cold acetone (25% final concentration, VWR, USA) for 1h at - 20°C followed by centrifugation (400x g ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells expressing opsin-membrane proteins were incubated in the dark during 1h at 4°C on a nutating mixer (VWR) to increase active rhodopsin complexes ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stored at 4°C while the incubator cooled to 18°C and then protein expression was induced with 1 mM IPTG (VWR) and expressed at 18°C overnight ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The proteins were detected by adding chemiluminescence detection reagent (ECL Amersham) to the membranes and exposing onto ECL Hyperfilm (VWR) for 30m.
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bioRxiv - Biophysics 2020Quote: ... the untreated and treated bacteria (expressing H-NS-mEos3.2 fusion proteins) were filtered by 0.2 μm filters (VWR International LLC), resulting in filtrates constituting leaked cellular contents due to membrane damage ...
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bioRxiv - Plant Biology 2023Quote: ... was added to deactivate the protein (45°C, 4 hours) and the DNA was purified through phase lock gel (VWR) and precipitated with 1/10 volume of 3 M Sodium Acetate (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody binding was visualized using the using BrightVision Goat anti Rabbit / Poly-AP and BrightVision Goat anti Mouse / Poly-HRP (VWR/ImmunoLogic), with DAB as chromogen (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... serum-free culture media containing secreted proteins were collected and concentrated in 0.5 mL 3-kDa molecular weight cut-off centrifugal filters (VWR North America). In some experiments ...
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bioRxiv - Microbiology 2023Quote: ... A total of 20 μg of proteins were mixed with 6x SDS loading dye containing 10% 2-mercaptoethanol (VWR Life Sciences) and boiled at 100 °C for 10 min before loading ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to a PVDF membrane for 1.5 H at 400 mA and blocked using 5 % BSA (VWR, 103219-864) rocking at room temperature for 1 hour ...
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bioRxiv - Microbiology 2019Quote: ... 2 µL of 1:10 diluted purified anti-SpB antibody1 and 2µL of Cy3 conjugated Goat Anti Rabbit IgG Polyclonal Antibody (VWR R611-104-122) were added to 40 µL of 0.4 O.D ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed 3x for 15 minutes with PBS-T (PBS with 0.02% Tween) and incubated for 2 hours with either anti-rabbit HRP-linked (VWR, cat. no NA934) or anti-mouse HRP-linked (VWR ...
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bioRxiv - Physiology 2022Quote: ... frozen and crushed in liquid nitrogen for protein and RNA extraction or ii) embedded in Tissue-Tek OCT compound (VWR, Mississauga, Canada) and frozen in isopentane cooled with liquid nitrogen for cryostat sectioning54 or iii ...
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bioRxiv - Cell Biology 2022Quote: ... Samples containing 20-50 µg protein were separated by SDS polyacrylamide gel electrophoresis and transferred to a nitrocellulose or polyvinylidene fluoride membrane (10600023, VWR, Radnor, PA). After blocking the membrane for 1h in 3% BSA in Tris- buffered saline with Tween 20 (TBS-T) ...
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bioRxiv - Bioengineering 2021Quote: Plant or mammalian produced SARS-CoV-2 RBD protein were diluted in sterile 1X PBS to 2 μg/mL and coated onto a 96-well plates (VWR #62402-959) (50 μL /well ...
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bioRxiv - Systems Biology 2023Quote: ... Enzyme solutions for +M and -M conditions were prepared in separate 8-tube PCR strips (VWR #732-1521 or low-protein binding) by mixing 25 μL reaction buffer (+M/-M ...
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bioRxiv - Cell Biology 2019Quote: ... the medium was refreshed and after additional 24 h virus was collected and filtered through a 0.45μm low protein binding membrane (VWR International, Radnor, PA, #28145-479). For dCas9-KRAB fusion protein expression and CRISPRi-mediated knockdown ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue sections were co-stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
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bioRxiv - Neuroscience 2023Quote: ... 2h,i,j,l,m,o) RNAscope was followed by standard immunofluorescent staining using rabbit-anti-RFP (Avantor VWR, RL600-401-379, 1:1000) as the primary antibody ...