Labshake search
Citations for VWR :
101 - 140 of 140 citations for IL 12 Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
-
bioRxiv - Bioengineering 2024Quote: ... These liver samples were homogenized with RIPA lysis buffer premixed with 1X protease and phosphatase-inhibitor cocktail and 1x EDTA (to prevent non-specific protease, metalloprotease, or phosphatase-mediated digestion) using a benchtop homogenizer (VWR VDI 12 Homogenizer). Samples were centrifuged at 4000 rpm for 10 minutes at 4 °C ...
-
bioRxiv - Microbiology 2020Quote: ... co-culture experiments were performed using membrane-separated 12-well tissue plate cultures containing 0.1 µm pore PET membranes (VWR® Cat. No. 10769-226). Briefly ...
-
bioRxiv - Neuroscience 2023Quote: ... Elma Schmidbauer GmbH), mixed (10 min, 2035 rpm, HeidolphTM MultiReax) and centrifuged (1 min, 12300 RCF, Micro-Star 12, VWR®, Radnor, PA, USA). The total protein concentration in each sample was determined using the BCA assay in 10-20 μl sample aliquots ...
-
bioRxiv - Neuroscience 2023Quote: ... Clarified lysate was warmed up at room temperature at least 12 hours prior to affinity purification and then sterile filtered using a vacuum bottle filter (VWR International LLC, 28199-778). AAVX and AAV9 columns were attached to an AKTA Pure 25 L chromatography system (Cytiva ...
-
bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
-
bioRxiv - Microbiology 2024Quote: ... Rpoβ (anti-RNA polymerase beta mouse monoclonal antibody [clone: 8RB13]; VWR) was diluted 1:20,000 ...
-
bioRxiv - Cancer Biology 2020Quote: ... RNA was isolated from mouse liver with peqGOLD RNAPure reagent (VWR, USA) and QIAshredder spin columns (Qiagen ...
-
bioRxiv - Physiology 2021Quote: Freshly dissected mouse tissues were rinsed in PBS (VWR, cat. No. 437117K) and then immediately transferred into ice-cold fixative solution of 4% paraformaldehyde (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... and IRDye 680RD Goat-anti-Mouse antibody (VWR, Cat No:102673-408) at 1:20,000 dilutions ...
-
bioRxiv - Immunology 2023Quote: Mouse tissues were homogenized using soft tissue homogenizing beads (VWR, Radnor, PA). Total RNA was isolated using TRIzol® (Life Technologies ...
-
bioRxiv - Microbiology 2023Quote: ... Six to seven grams (6 ml) of soil was transferred to each well of the 12-well microtiter plates (VWR American cat no.:10861-556, USA), containing three one-millimeter holes in the bottom of all wells for drainage ...
-
bioRxiv - Cell Biology 2023Quote: ... donkey anti-mouse IgG conjugated to IRDye 800CW (VWR, cat. no. 102673-332) or goat anti-rabbit IgG conjugated to IRDye 680RD (VWR ...
-
bioRxiv - Molecular Biology 2023Quote: ... Nitrocellulose membrane was then incubated with goat Anti-mouse (102971-154, VWR International) and goat Anti-rabbit (103011-498 ...
-
bioRxiv - Immunology 2024Quote: ... Mouse tumor sections were mounted on microscope cover glasses (#631-0170, VWR, France) using Fluoromount-G (#00-4958-02 ...
-
bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium ...
-
bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
-
bioRxiv - Cancer Biology 2020Quote: ... mouse anti-cavin3 (Cat no. ABNOH00112464-MO4, 1:200 PLA, VWR International Pty, Australia), rabbit anti-BRCA1 C-20 (SC-642 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Drug free mouse plasma and human plasma with lithium heparin were purchased from VWR International (PA ...
-
bioRxiv - Cell Biology 2023Quote: Mouse skin and wound beds were embedded in optimum cutting temperature (OCT) compound (VWR) and wound beds were sectioned through their entirety to identify the center ...
-
bioRxiv - Bioengineering 2023Quote: ... Mouse uterus cross-section tissue was fixed for 30 minutes in 4% formaldehyde (VWR) before blocking for 30 minutes in 10% Normal Goat Serum (50062Z ...
-
bioRxiv - Neuroscience 2020Quote: Mouse brains were collected through flash freezing in OCT Tissue Tek medium (VWR #25608-930) in dry ice ...
-
bioRxiv - Molecular Biology 2023Quote: Mouse and chicken brains were dissected and immediately immersed in OCT embedding medium (361603E, VWR) for a washing step ...
-
bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated donkey antirabbit (NA934V) and anti-mouse (NA931-1M (both from VWR International Ltd.) secondary antibodies were used at 1:3000-5000 ...
-
bioRxiv - Neuroscience 2023Quote: ... mouse brains were first fixed in 4% paraformaldehyde (PFA) in PBS solution (VWR, Cat# ALFAJ61899.AK) for 24 h ...
-
bioRxiv - Cell Biology 2020Quote: ... coverslips were probed with mouse anti-α-tubulin antibody (DM1A, VWR; #PI62204; 1:1000 in blocking buffer) for 1h at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: ... and cultured in an optimized mouse prostate primary cell medium composed of DMEM/F12 (VWR, 45000-344), 10% FBS ...
-
bioRxiv - Genomics 2019Quote: ... Western blots were performed using a mixture of mouse anti-actin antibodies (VWR catalog number 89500-294) and rabbit anti-TAP antibodies (ThermoFisher catalog number CAB1001 ...
-
bioRxiv - Bioengineering 2020Quote: The mouse hearts were first decellularized in 0.25% and 0.5% (wt/vol) sodium dodecyl sulfate (SDS, VWR) solution at RT for 24 h or until the tissues turn transparent white with constant agitation ...
-
bioRxiv - Cancer Biology 2022Quote: ... tumor-bearing mouse brains and flank tumors were homogenized in a pre-chilled glass tissue grinder (VWR) with RIPA lysis buffer containing protease and phosphatase inhibitors (ThermoFisher Scientific) ...
-
bioRxiv - Cell Biology 2021Quote: ... mouse-keratinocyte co-cultures were washed twice with cold PBS and then fixed with 4% paraformaldehyde (VWR 1.04005.1000) for 20 min at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... Membranes were then rinsed 3x in TBS and incubated for 1 hour in anti-mouse IR680 (VWR 102971-020) and anti-rabbit IR800 (VWR 103011-494 ...
-
bioRxiv - Cell Biology 2019Quote: ... The donkey anti-rabbit (NA934, 1:1000) and anti-mouse (NA931, 1:1000) horseradish peroxidase (HRP)-linked secondary antibodies were purchased from VWR. The GFER rabbit polyclonal antibody (#HPA041227 ...
-
bioRxiv - Developmental Biology 2023Quote: ... using the mouse MF20 (1:500, DSHB), the rabbit anti-GFP (1:1000, Life technology) and the rabbit RFP (1:1000, VWR) primary antibodies ...
-
bioRxiv - Genomics 2023Quote: ... then incubated on rollers at room temperature for 1 hour with an anti-mouse secondary antibody conjugated to horseradish peroxidase (VWR, NXA931). After four further washes for 10 min each (2 x blocking buffer ...
-
bioRxiv - Cancer Biology 2023Quote: ... Antibody binding was visualized using the using BrightVision Goat anti Rabbit / Poly-AP and BrightVision Goat anti Mouse / Poly-HRP (VWR/ImmunoLogic), with DAB as chromogen (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2021Quote: ... Immunoblots were then washed with TBS containing 0.05% Tween 20 (TBST) and incubated for 1 hour at ambient temperature with either anti-mouse horseradish peroxidase conjugated (NA931, VWR; 1:5000); or anti-rabbit HRP conjugated (NA934 ...
-
bioRxiv - Bioengineering 2024Quote: ... AB_11125142) or an anti-mouse secondary antibody (1:3000, Cat# 170-6516, RRID: AB_11125547) and enhanced chemiluminescence reagent (VWR, Cat # 490005-018). The signal was visualized using a C-DiGit Blot Scanner (Li-Cor) ...
-
bioRxiv - Immunology 2020Quote: ... Fused cells were then seeded on 96 well flat bottom plates onto mouse peritoneal cells as feeder layer in hypoxanthine-aminopterine-thymidine (VWR, Radnor, Pennsylvania, USA) medium for the selection process.