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Citations for VWR :
101 - 150 of 270 citations for DNA Mismatch Repair Protein Msh3 MSH3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... DNA-RNA hybrids were eluted and treated with Proteinase K (VWR Scientific). Eluted DNA was subsequently isolated by phenol:chloroform:isoamyl alcohol extraction and DRIP-qPCR was performed on Myocardin promoter and control locus and results were calculated as per Sanz and Chédin ...
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bioRxiv - Microbiology 2024Quote: ... The precipitated DNA was resuspended in TE buffer (VWR, Radnor, PE, USA) and purified using QIAamp DNA Mini Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were sorted into DNA LoBind® Eppendorf tubes (VWR, #525-0130), then CD45-cells were mixed with CD45+ cells at 2:1 to 10:1 ratios depending on cell availability to enrich for glioblastoma cells ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins in the pellet were solubilized by sonication (incubation in Ultrasonic Cleaner bath, VWR) for 10 min followed by incubation for 60 min at 4 °C under rotation ...
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bioRxiv - Biochemistry 2023Quote: ... protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG, #437145X, VWR) at 16 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration throughout the study was determined via Bradford assay (catalogue number-VWR M172).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using Amersham ECL Prime Western Blot Detection Reagent (VWR, CA89168-782) and a ChemiDoc Imaging System (Bio-Rad Laboratories).
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bioRxiv - Genetics 2024Quote: ... then protein was lysed and harvested in 1X RIPA lysis buffer (VWR Life Science) supplemented with 1X cOmpleteTM Mini EDTA-free protease inhibitor cocktail (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... Protein absorbance was read at 562 nm using a Spectramax i3x Microplate reader (VWR). 10 µg of total proteins were denatured at 95°C for 5 min in 100 mM dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins separated on SDS-PAGE gels were transferred to nitrocellulose membranes (VWR, #27376-991) and then probed with appropriate antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... genomic DNA was extracted by lysing with DirectPCR Lysis Reagent Cell (Peqlab, VWR) supplemented with 0.5 μg/μl Proteinase K (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The DNA pellet was washed with 70% ethanol (VWR, Cat. No. 71006-012) twice and air dried ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was precipitated by adding an equal volume of isopropanol (VWR, Radnor, PA) and incubated at −20°C for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... 0.33 mM of each dNTP and 1 U of Taq DNA polymerase (VWR) using one cycle of 94°C for 5 min 30 s ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was extracted from cortical tissue using the TriFast™ reagent protocol (VWR) and the Cacna1c gene was amplified with the PfuPlus! DNA Polymerase (Roboklon) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA quality was examined via gel electrophoresis on a 0.5% agarose gel (VWR) with 0.1% GelRed (10000x ...
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bioRxiv - Neuroscience 2022Quote: ... 0.33 mM of each dNTP and 1 U of Taq DNA polymerase (VWR) using one cycle of 94°C for 5 min 30 s ...
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bioRxiv - Plant Biology 2022Quote: ... PCR was performed with Red Taq DNA Polymerase Master Mix (VWR Life Science) according to the manufacturer’s instructions and purified with magnetic beads (CleanNGS) ...
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bioRxiv - Genomics 2020Quote: DNA was isolated by grinding with a disposable homogenizer pestle (VWR, Radnor PA, USA) on ice for 30 minutes followed by DNA extraction with the Qiagen DNeasy extraction kit (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... The agarose was supplemented with peqGreen DNA/RNA dye (peqlab brand, VWR, Oslo, Norway) for visualization ...
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bioRxiv - Synthetic Biology 2023Quote: ... Karyotyping PCRs were conducted using RedTaq DNA Polymerase 2X Master Mix (VWR Life Science) with the following published primers ...
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bioRxiv - Pathology 2024Quote: ... HRP-conjugated secondary antibodies (NA9340, VWR International GmbH, Darmstadt, Germany) and chemiluminescent substrate (WesternBright Chemilumineszenz Substrat Quantum ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody binding was visualized with DAB-nickel (VWR, 101098-434). Sections were counterstained with nuclear fast red ...
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bioRxiv - Molecular Biology 2020Quote: ... and recombinant protein expression was induced by addition of 0.5 mM isopropyl-β-D-thiogalactopyranoside (VWR) for 16–18 h at 25°C ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmid DNA was amplified and purified using EZNA endo-free plasmid maxi kit (VWR International).
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bioRxiv - Cell Biology 2021Quote: Cells were transfected with 4 µg DNA: 4 µL JetOPTIMUS transfection reagent (VWR, 76299-634) according to manufacturer guidelines in standard medium overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA samples (1ug) were then separated on a 0.45% agarose gel (Biotechnology VWR, life science) at 30V 18-20 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was digested according to the peqGOLD DNase I Digest kit (VWR 13-1091-01), followed by washing steps with 400 µL RNA Prep Buffer (ZymoResearch R1060-2 ...
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bioRxiv - Cell Biology 2023Quote: ... DNA samples (1ug) were then separated on a 0.45% agarose gel (Biotechnology VWR, life science) at 30V 18-20 hours in cold room ...
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bioRxiv - Genetics 2023Quote: ... DNA was cleaned with phenol-chloroform phase separation with phase lock tubes (VWR, 10847-802). DNA was precipitated with 1 volume isopropanol ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cell/DNA mix was then transferred to a pre-chilled 2 mm cuvette (VWR) and pulsed in a GenePulser Xcell (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR) for cohesin trimers and a HiLoad 16/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Protein cell lysates were collected by lysing and scraping cells with RIPA Lysis buffer (VWR, Mississauga, Ontario) mixed with protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... Then we denatured the phage protein coat by adding 7.5 ml of 3 M KOAc (VWR international), pH 5.5 ...
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bioRxiv - Neuroscience 2023Quote: ... The dots were normalized on protein concentration (BCA assay) and cell numbers (EVE Automated Cell Counter, VWR). Each drop contained 10ug protein in 5 ul RIPA ...
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bioRxiv - Biochemistry 2023Quote: ... at which point protein expression was induced by adding isopropyl β-D-1-thiogalactopyranoside (IPTG) (VWR, #437145X) at the final concentration of 0.5 mM ...
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bioRxiv - Biophysics 2022Quote: ... proteins were purified via size exclusion chromatography using a Superose 6 increase 10/300 GL column (VWR). Peak fractions were collected ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to nitrocellulose overnight in 20% methanol in Tris/Glycine transfer buffer (VWR, #97061-382). Total protein was visualized using the LI-COR REVERT™ Total Protein Stain (Licor ...
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bioRxiv - Biophysics 2021Quote: ... Cell culture media growth factors and antibodies were purchased from VWR International PBI (Milan ...
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bioRxiv - Cell Biology 2023Quote: ... IRDye 800CW Goat-anti-Rabbit antibody (VWR, Cat No:102673-330) and IRDye 680RD Goat-anti-Mouse antibody (VWR ...
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bioRxiv - Microbiology 2024Quote: ... Rpoβ (anti-RNA polymerase beta mouse monoclonal antibody [clone: 8RB13]; VWR) was diluted 1:20,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... incubated with the secondary antibody and DAPI (1:1000; AppliChem, VWR) for 1 h at RT and mounted with Prolong™ Gold Antifade reagent (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2022Quote: ... We then transferred the column to a labeled DNA LoBind Eppendorf 1.5 mL tube (VWR # 022431021), added 20 uL nuclease-free H2O ...
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bioRxiv - Systems Biology 2022Quote: ... paired gRNA entry vectors were created by PCR amplification (Red Taq DNA Polymerase Master Mix, VWR Life Science or iProof High-Fidelity DNA Polymerase ...
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bioRxiv - Genetics 2023Quote: ... DNA was cleaned and isolated with phenol-chloroform extraction using phase-lock tubes (VWR, 10847-802). 5 μg was run on a 1.5% agarose gel to ensure fragments of 200-400 bp were generated ...
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bioRxiv - Biophysics 2024Quote: ... and solutions of eluted DNA-encoded compounds were transferred to a 96-well PCR plate (VWR).
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bioRxiv - Cell Biology 2020Quote: ... the medium was removed and filtered through a 0.45-µm low-protein binding membrane (VWR International, Radnor, PA). McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2021Quote: ... all proteins were diluted in filtered (sterile syringe filters 0.45 μm cellulose acetate membrane, VWR International, Radnor, USA) Min buffer in the presence of either 0.5 mM ADP or ATP ...
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bioRxiv - Genetics 2020Quote: Quadriceps from P10 pups were dissected and total proteins extracted using radioimmunoprecipitation assay (RIPA) buffer (VWR, Radnor, PA) supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Enzyme solution was prepared in distilled water and the protein concentration was determined using Bradford reagent (VWR, USA) with bovine serum albumin as the protein standard ...