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Citations for VWR :
1401 - 1450 of 1451 citations for 6 Bromo 3 phenyl imidazo 1 2 a pyridine 2 carboxylic acid methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... and 1/5th of the lysate was added into 40 µL lysis buffer and sheared in a sonicator (VWR Aquasonic 250D, 35Khz) for 3 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was changed and the incubation continued for another 24 hours before addition of 1-5 µg/mL puromycin (VWR 97064-280). Incubation with puromycin continued for several days until untransfected control wells were depleted of cells ...
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bioRxiv - Neuroscience 2021Quote: ... After which cells were washed once with 1x PBS with 1% BSA before being filtered through 40um FlowMi Tip Strainers (VWR, 10032-802). Cells were then diluted with 1x PBS with 1% BSA and loaded onto the Chromium 10x Controller according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of each reaction were mixed with loading dye and analyzed on a 1% (w/v) agarose gel containing GelRed (VWR, Darmstadt, Germany). The remaining PCR mix was purified using a QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... the tissue was first blocked for 30 min using a blocking buffer (TBS with 1% BSA (VWR, 22013, Bovine Serum Albumin (BSA), fraction V ...
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bioRxiv - Immunology 2021Quote: ... Whole blood cell lysates or lysates of PBMCs from IL-1-Ra-Ab positive patients were treated with alkaline phosphatase as previously described using FastAP thermosensitive alkaline phosphatase (Fermentas/VWR, Darmstadt, Germany).(40 ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were stopped by incubating on ice for 5 min and digesting with 1 µg of Proteinase K Solution (VWR, PA, USA) for 15 min at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... both supplemented with 10 μg/mL chloramphenicol (Cm) and 10 μM (mild induction) or 100 μM (strong induction) of isopropyl β-D-1-thiogalactopyranoside (IPTG) (43714 5X, VWR Chemicals) when necessary.
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bioRxiv - Bioengineering 2020Quote: ... and particles were removed from PDMS chip and glass substrate (76 x 26 x 1 mm microscope slides, VWR International GmbH, Germany) via isopropanol washing and drying with pressurized air ...
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bioRxiv - Cancer Biology 2021Quote: ... was achieved using an adapted protocol from Ly et al.22 FFPE TMAs were first washed twice for 1 minute in a xylene bath (mixture of isomers; ≥98.5%, AnalaR NORMAPUR®, VWR, Leicestershire, UK). Residual xylene was removed ...
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bioRxiv - Microbiology 2019Quote: ... One single colony was picked and incubated for 8 to 10 h at 30°C in either 4.5 ml or 1 ml BHI containing 154mM acetate in reaction tubes or Deepwell-plates (VWR International, Pennsylvania, USA), respectively ...
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bioRxiv - Microbiology 2019Quote: ... 2 µL of 1:10 diluted purified anti-SpB antibody1 and 2µL of Cy3 conjugated Goat Anti Rabbit IgG Polyclonal Antibody (VWR R611-104-122) were added to 40 µL of 0.4 O.D ...
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bioRxiv - Immunology 2020Quote: One mL of BMDM from lipin-1mKO and littermate control mice at a concentration of 1×106/mL was placed into falcon tubes (VWR-60818-500). Cells were either treated with or without IL-4 (40ng/mL ...
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bioRxiv - Immunology 2020Quote: ... Unbound protein was washed away with PBST (1x PBS with 0.5% Tween) followed by blocking with 1% casein (w/v) in PBS (VWR International, Radnor, PA) for 2 h at room temperature with mild shaking (55 rpm) ...
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bioRxiv - Microbiology 2022Quote: ... 1 cell line was cultured at 37°C with 5% CO2 in DMEM with high glucose and L-glutamine (VWR 16777-129) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each fin bud was first homogenized in 50 μL of Buffer RLT (supplemented with β-mercaptoethanol per kit recommendation) for 1 min at RT in a 1.5 mL centrifuge tube using a cordless motor (VWR Catalog # 47747-370) and pestle (USA Scientific Catalog # 1415-5390) ...
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bioRxiv - Plant Biology 2023Quote: ... from accessions grown in UC Davis or UC Riverside greenhouses and floated overnight in 1 mL deionized water in a 24-well tissue culture plate (VWR #10062-896) with a plastic lid to prevent evaporation of the water ...
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bioRxiv - Bioengineering 2023Quote: ... the strips were immersed in a blocking buffer (1% BSA and 0.05% Tween 20) for one hour on an orbital shaker (no. 57018-754VWR; VWR International, Brisbane, CA) set at 150 rpm and thereafter dried overnight at 4° Celsius ...
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bioRxiv - Cancer Biology 2023Quote: ... Each well is washed with 1 mL of phosphate buffered saline (PBS) prior to fixation with 500 µL of 10% buffered formalin (VWR, 89379-094). After at least 24 hours of fixation ...
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bioRxiv - Cell Biology 2023Quote: ... flies were then transferred to new vials containing a 1cm by 5cm strip of filter paper saturated with 300µl of 30% H2O2 (VWR, Cat# BDH7690-1) and placed at 25°C for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were seeded at 1×106 cells/well in 24-well plates and maintained in X-VIVO-15™ media (CA12001-988, VWR, USA). Four treatment groups were used ...
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bioRxiv - Cell Biology 2023Quote: ... washed cells were suspended at a concentration of 1 × 106 cells/mL in the growth medium and filtered using a 40 µm cell strainer (VWR: 10199- 655). Cells were then sorted into 15 mL collection tubes using a sterile 100 µm chip (Sony ...
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bioRxiv - Bioengineering 2024Quote: ... AB_11125142) or an anti-mouse secondary antibody (1:3000, Cat# 170-6516, RRID: AB_11125547) and enhanced chemiluminescence reagent (VWR, Cat # 490005-018). The signal was visualized using a C-DiGit Blot Scanner (Li-Cor) ...
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bioRxiv - Cell Biology 2020Quote: ... Solutions were mixed by shaking the compound plate for 1 min at room temperature at 1200 rpm using a microplate shaker (cat. 12620-926, VWR, West Chester, PA). A typical plate layout map used for screening experiments is shown in Figure 1 ...
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bioRxiv - Biophysics 2019Quote: ... cells were grown in 6-well plates (STARLAB; Milton Keynes, UK) or 30 mm culture dishes (STARLAB) on untreated 30 mm borosilicate coverslips (Thickness # 1, VWR International; Radnor, PA) or on poly-D-lysine coated FluoroDishes (FD35-PDL-100 ...
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bioRxiv - Biophysics 2020Quote: ... The sheath and sample fluid syringes (BD Luer-Lok™ 5-ml syringe #613-2043P and BD Luer-Lok™ 1-ml syringe #613-4971; VWR, Germany) were connected to the fabricated chip via FEP Tubing (1/16” OD ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 24 h starved polyps were incubated in 1 mM linalool for 10 min in a 60 mm tissue culture dish (VWR International, Radnor, PA). Each animal was pinched using a pair of Dumont No ...
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bioRxiv - Bioengineering 2019Quote: ... and β3TUB is a tubulin thought to be specifically involved during differentiation of neuronal cell types [69].) Antibodies were diluted in antibody dilution buffer (PBS containing 1% bovine serum albumin (VWR, Radnor, PA, USA) and 0.3% Triton X-100 (Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2021Quote: ... and 4% TCA (100μl) were added to these tubes and vortexed for 1 minute on a multi-tube vortex mixer (VWR International LLC, Radnor, PA).
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bioRxiv - Neuroscience 2022Quote: ... mEPSCs were recorded at a holding potential of −70 mV in the presence of 50 μM PTX and 1 μM tetrodotoxin (TTX; VWR, Catalog#89160– 628). Electrophysiological data were analyzed using Clampfit 10.5 (Molecular Devices ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were mixed with the scintillation liquid and counted in a scintillation counter (Packard TriCarb Model, 1 min/vial) using Solvent-Saver™ scintillation vials (VWR International LLC.).
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% (HeLa) or 15% (Tig-1) fetal bovine serum (HyClone, GE Healthcare Life Sciences, Marlborough, MA; Avantor Seradigm, VWR, Radnor, PA), 100 units/mL penicillin and 100 µg/mL streptomycin (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of the enriched seawater and 100 μL of the diatom-concentrated seawater were then inoculated on agar plates (1% agar – VWR Agar for bacteriology) supplemented with Marine Broth 2216 (VWR ...
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bioRxiv - Immunology 2023Quote: ... The wells were emptied and blocked with 300 µL of PBS containing 1% BSA (Bovine Serum Albumin Fraction V, VWR Cat# 97061-416) for 1 h at 25°C ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was extracted from the eluted beads with phenol: chloroform: iso–amyl alcohol (25:24:1) (v/v/v) (VWR, 136112– 00–0), followed by precipitation with 1:1 (v/v ...
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bioRxiv - Genetics 2023Quote: ... with a scalpel on a cold block (Figure 1b) and resuspended on ice in 1 ml of lysis buffer containing 0.32 M Sucrose (VWR Catalog No. 97061-432), 10 mM Tris-HCl pH 8 (Invitrogen REF 15568-025) ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue sections were co-stained overnight at 4°C with a primary chicken antibody against the astrocyte marker Glial Fibrillary Acidic Protein (GFAP) (1:1000 in blocking solution; Cat No. 200-901-D60, VWR, Radnor, PA, USA) and a primary rat antibody against myelin basic protein (MBP ...
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bioRxiv - Cancer Biology 2023Quote: ... Appropriate number of acinar cells were then resuspended in premade collagen solution (1 mg/ml rat tail type1 collagen (VWR, Cat. No. CACB354236), 10× Waymouth’s media (Cedarlane Labs ...
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bioRxiv - Neuroscience 2020Quote: ... which consists of three steps: 1) Cells were seeded into uncoated T75 flasks (4×106 viable cells/flask, VWR, Cat# 734-2788, Radnor, PA) for 18 hrs to remove fibroblasts ...
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Exploring the interspecific interactions and the metabolome of the soil isolate Hylemonella gracilisbioRxiv - Microbiology 2022Quote: ... was added to the surface of the 1/10th TSBA plates and grown bacterial cells were suspended from the plate surface with a disposable cell scratcher (VWR international B.V., the Netherlands). For total RNA extraction the obtained cell suspension was transferred to a tube containing RNA Protect Bacteria Reagent (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... was mixed with deuterium oxide (450 µl) containing sodium formate (1 µmol/L) and then passed through a 10 KDa cutoff centrifugal filter (VWR, Radnor, PA, United States). 1H-NMR spectra were acquired using a 600 MHz spectrometer (Bruker AVANCE II ...
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bioRxiv - Synthetic Biology 2020Quote: ... For that purpose specially designed single-use imaging chambers were made using 18 mm × 18 mm cover glasses #1 (VWR, Cat No. 631-1331) and silicon seals cut out of ultra thin silicone film with 0.3 mm thickness (Silex ...
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bioRxiv - Microbiology 2019Quote: ... Colonies of each bacterial test isolate and a positive and negative control isolate were harvested with a 1 μl loop (VWR International, catalog # 12000-806) from blood agar (BD Diagnostics Systems ...
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bioRxiv - Neuroscience 2021Quote: ... Ribbons were deparaffinized by heating at 65°C for 1 h followed by washes in histoclear I and II (VWR; 101412-876, 10141-8822) for 5 m ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Single colonies were grown overnight in 10 mL YPGal+Dex and genomic DNA was isolated from 1 mL aliquots using the VWR® Life Science Yeast Genomic DNA Purification Kit (VWR 89492-616, us.vwr.com).
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bioRxiv - Neuroscience 2023Quote: ... Elma Schmidbauer GmbH), mixed (10 min, 2035 rpm, HeidolphTM MultiReax) and centrifuged (1 min, 12300 RCF, Micro-Star 12, VWR®, Radnor, PA, USA). The total protein concentration in each sample was determined using the BCA assay in 10-20 μl sample aliquots ...
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bioRxiv - Microbiology 2023Quote: ... salinarum were generated by isothermal Gibson assembly (Gibson, 2011) of: 1) the PCR fragments (amplified by KAPA Biosystems DNA polymerase [VWR] and gel extracted); and 2 ...
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bioRxiv - Neuroscience 2023Quote: ... 2h,i,j,l,m,o) RNAscope was followed by standard immunofluorescent staining using rabbit-anti-RFP (Avantor VWR, RL600-401-379, 1:1000) as the primary antibody ...
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bioRxiv - Microbiology 2023Quote: ... The suspension was allowed to cool at room temperature (RT) for 10 min before adding 1 µl RNAse at 10 mg/ml (VWR cat no. 0675-250MG) and incubation at 37 for 60 min ...
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bioRxiv - Cell Biology 2022Quote: ... then spheroplasted in digestion mix (8.9% glusulase [part no. NEE154001EA, PerkinElmer] and 1 mg/mL 100T zymolyase [cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR] in 1.2M sorbitol-citrate) by rotating at 30°C for 4-5 hours (digestion was assessed by looking at cells on a light microscope) ...