Labshake search
Citations for VWR :
1401 - 1450 of 1496 citations for 2 4 6 Trichlorophenol 13C6 99% 100 Ug Ml In Isooctane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... 50 μL of mAbs diluted to 20 μg/mL in PBS were added to the first well of a 96-well U-bottom plate (VWR), and diluted two-fold in PBS for 25 μL mAb total per dilution ...
-
bioRxiv - Biochemistry 2022Quote: ... The 500 mL culture (OD550 ∼0.6) was then transferred into the carboy via sterile rubber transfer line (VWR-62993-726), 18-gauge needles (Becton Dickinson 305196 ...
-
bioRxiv - Genomics 2020Quote: ... Cells expressing Cas9 were selected either by sorting for GFP10 or selection with 350 ng/ml Puromycin dihydrochloride (VWR J593) for three consecutive days starting one day after electroporation17 ...
-
bioRxiv - Biochemistry 2019Quote: HDL samples prepared by adding 15 μL 3 mg/mL spin-labeled apoA-I probe to 45 μL 1 mg/mL HDL and drawn into an EPR-compatible borosilicate capillary tube (VWR) (32) ...
-
bioRxiv - Bioengineering 2020Quote: ... ⅘ of the 40% layer and ⅕ of the 54% layer were extracted from the polyallomer Quick-seal ultracentrifuge tube (Fisher) with an 18-gauge needle (Fisher) attached to a 10-mL syringe (VWR). The collected virus fraction was diluted in an equal volume of PBS + 0.001% Tween-20 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cell suspension was filtered through a 35µm cell strainer into a 5 ml falcon round bottom tube (VWR #21008-948) for sorting using a MoFlo XDP cell sorter fitted with a 100µm nozzle ...
-
bioRxiv - Bioengineering 2020Quote: ... either D-luciferin (Fisher #LUCK-2G; 150 µg / mL read concentration) or Rluc substrate (VWR # PAP1232; 17 µM read concentration). Maximum cytotoxicity was defined as luminescent signal from wells containing only media while no cytotoxicity was defined by wells containing only target cells ...
-
bioRxiv - Biophysics 2020Quote: ... the diluted protein was loaded to BSA (200 μl of 1 mg/ml for 30 seconds) treated coverslips (no. 1.5H precision cover slides, VWR Marienfeld), minimizing fluorophore interactions with the glass slide ...
-
bioRxiv - Cell Biology 2021Quote: 3,000 ESCs/well mixed with 0.3 μg Wnt3a-beads/well were seeded onto human fibronectin (10 μg/mL, cat. um. 7340101, VWR/SLS) coated 8-well slides (cat ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Flies were then returned to the fridge at 11 °C in individual containers (OD: 33mm, 60 ml, VWR international, USA) with 50 µl of distilled water on piece of filter paper.
-
bioRxiv - Immunology 2022Quote: ... mAbs diluted to 50 μg/mL in PBS were added to the first well of a 96-well U-bottom plate (VWR), and diluted five-fold in PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... overnight media was replaced either with EtBr-containing media (DMEM + 10% FBS + 0.2 μg/ml EtBr (VWR life science, X328) + 50 μg/ml uridine ...
-
bioRxiv - Neuroscience 2022Quote: ... clear-bottomed, 96-well plates (Falcon, Switzerland) at a density of 200,000 cells/mL or onto coverslips (CS) (VWR, Switzerland) at a density of 250,000 cells/mL ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... yeast cultures were diluted to OD600=0.67 in 5 mL SGDCAA (6.7 g/L YNB without amino acid VWR #90004-150), 5 g/L ammonium sulfate (Sigma-Aldrich #A4418) ...
-
bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed in RPMI before resuspension in buffer (0.2% FBS in PBS) at concentration of 1 × 106 cells/ml in FACS tubes (Falcon® Brand, VWR). Before addition of the cocktail of 21 monoclonal antibodies ...
-
bioRxiv - Microbiology 2022Quote: ... All discs were subsequently placed into 1 mL of neutralising buffer (Becton Dickinson, Detroit, MI, USA) and vortexed with glass beads (2.5 - 3.5 mm; VWR, Leicestershire, UK) for 1 min to disrupt the biofilm ...
-
bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated overnight at 23°C in HL-5 medium supplemented with 200 µM sodium palmitate ...
-
bioRxiv - Microbiology 2022Quote: ... albicans were cultured at 37 °C with aeration in 5 mL of YPD (10g/L Yeast Extract (VWR J850-500G), 20g/L Peptone (VWR J636-500G) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... SbU- +bts1Δ and SbU- +bts1Δ + thi6Δ strains were incubated in 2.6 mL YPD in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen ...
-
bioRxiv - Microbiology 2023Quote: ... PBS was added to wells (1 mL) and the base and sides of each well were scraped twice using a cell scraper (VWR) to disrupt biofilm ...
-
bioRxiv - Microbiology 2022Quote: ... discoideum Ax3 or Δsey1 amoebae were seeded at a density of 1×105 cells/ml in cell culture-treated 96-wells plates (VWR) and cultivated at 23°C in HL-5 medium ...
-
bioRxiv - Genomics 2023Quote: ... 5000 cfu were plated in 1 mL media in each of 21 wells of a 24-well culture plate (10861-558, VWR), and initial cultures were plated for cfu on 7H10 + OADC to confirm number of cells in initial culture ...
-
bioRxiv - Microbiology 2023Quote: ... The lentivirus-containing medium was then removed and cells were allowed to recover for 72 hours whereupon cells were passaged in the presence of 1 μg/mL puromycin (VWR) and remained under selection for 3 days.
-
bioRxiv - Developmental Biology 2023Quote: ... Egg chambers were dissected and manually dechorionated in isolation buffer and then incubated for 30 min in Zalokar’s buffer containing 4.5% hydrogen peroxide and 1 mg/ml horseradish peroxidase (VWR, Radnor, PA) before bleaching as above.
-
bioRxiv - Immunology 2023Quote: ... Monocytes were enriched by negative selection using StemCell RosetteSep Human Monocyte Enrichment Cocktail (StemCell 15068; 75μl per ml of blood) and Ficoll-Paque (VWR 17144003). Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034 ...
-
bioRxiv - Genomics 2023Quote: ... followed by trituration of brains in 5 ml of DMEM complete media using a glass Pasteur pipette (VWR, 14672-380). The triturated tissue was resuspended of DMEM complete media and filtered through a 40 μm nylon cell strainer (Corning ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were transformed with gene-specific linear DNA and selected on Kanamycin (50 µg/mL) (USP, VWR Life Science, USA) plates for gene deletion ...
-
bioRxiv - Microbiology 2023Quote: ... cells were washed and 10 ml of serum-free DMEM containing 0.2% w/v bovine serum albumin (BSA; VWR international) and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich ...
-
bioRxiv - Developmental Biology 2023Quote: ... Bulk samples including bone and bone marrow were cryo-pulverized and resuspended in 1 ml ice-cold TriFast (VWR International) and carefully vortexed (30 sec) ...
-
bioRxiv - Microbiology 2023Quote: ... Then 20 μL of hemolymph per each sample were digested with 0.5 mL of 32% ultrapure hydrochloric acid (VWR Chemicals) under heating conditions (60 °C ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were washed off the filter using 5 mL ice-cold methanol/ethanol/chloroform 1:3:1 (methanol: HiPerSolv Chromanorm, VWR Chemicals, Dresden, Germany; ethanol: HiPerSolv Chromanorm, VWR Chemicals ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were washed off the filter using 5 mL ice-cold methanol/ethanol/chloroform 1:3:1 (methanol: HiPerSolv Chromanorm, VWR Chemicals ...
-
bioRxiv - Systems Biology 2023Quote: ... The metabolite extracts were transferred into 0.3 mL HPLC autosampler vials with inserts (VWR International LLC, Cat. 9532S-1CP-RS) and stored in −80°C until analysis.
-
bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended with base HdB-Tyl and used as inoculum for new cultures in a total volume of 40 mL in 250 mL Erlenmeyer flasks (50 mm Ø) with starting OD600 of 0.1 and closed with breathable rayon film (VWR). The cultures were monitored using the Cell Growth Quantifier (Aquila Biolabs ...
-
bioRxiv - Cell Biology 2021Quote: ... 0.5M EDTA) and lysed in SEMZ buffer (1M Sorbitol, 50mM EDTA, 28mM β-Mercaptoethanol and 2mg/ml Zymolyase 100T (IC320932 VWR)) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... An approximate volume of 100-200 µL of BUD-ELMs were collected into a 1.5 mL centrifuge tube and spun for 10 sec at 3200 rcf with a mini centrifuge (VWR®, C0803). This allowed for the material to collect at the bottom as an homogeneous paste ...
-
A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Coating was performed for 1 h with 15 μg/ml of fibronectin or with 3.5 μg/cm2 Cell-Tak (VWR, 734-1081) diluted in 0.1 M sodium bicarbonate ...
-
bioRxiv - Microbiology 2019Quote: ... in 500 mL of D2O (Cambridge Isotopes laboratories (Tewksbury, MA)) and adjusted to pH 7.4 with 1M NaOH (VWR, Darmstadt, Germany) and HCl (VWR ...
-
bioRxiv - Neuroscience 2019Quote: ... serum-free culture media containing secreted proteins were collected and concentrated in 0.5 mL 3-kDa molecular weight cut-off centrifugal filters (VWR North America). In some experiments ...
-
bioRxiv - Microbiology 2020Quote: ... Two-µl spots of each dilution were plated on selective (20 µg/mL thiamphenicol) and unselective square (90×15 mm, VWR international) BHI plates with or without 200 ng/mL anhydrotetracycline (ATc) ...
-
bioRxiv - Immunology 2019Quote: ... The tissues were mashed using the plunger of a 10 ml syringe on a 40 micron nylon filter (VWR; 10199-654). The filters were washed with MACS buffer (DPBS with 2% FBS ...
-
bioRxiv - Bioengineering 2022Quote: ... containing 20% FBS (Wisent, 80450, lot 115714), 1% Penicillin-Streptomycin solution (Wisent, 450-201-EL) and 2,5 ng/mL bFGF (VWR, 10821-962) in a 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... are suitable for screening 1.5 ml raw sample in deep-well plates with a working volume (wV) of 2.2 ml (e.g. 732-0612, VWR, Radnor PA US). All incubations should be performed under optimal host conditions (media and temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... The lysate was centrifuged at 14000×g for 40 min and a Macrosep advanced filter (3000 Da MW cutoff, 20 ml; catalogue no. 89131-974; VWR, USA) was used to remove the free biotin ...
-
bioRxiv - Synthetic Biology 2022Quote: ... for 30 mins in 150 μl lysis buffer containing 8 mg/mL lysozyme (from chicken egg white, #76346-678, VWR, USA), 10 mM Tris HCl pH 7.5 ...
-
bioRxiv - Neuroscience 2022Quote: Fresh fecal pellets were obtained aseptically by direct defecation of rats into a sterile 120-ml container (VWR, Radnor, PA, USA). Samples were collected from the 153 rats at PD 60 ...
-
bioRxiv - Neuroscience 2023Quote: ... and 1 mL of supernatant was transferred to a borosilicate glass culture tube pre-cleaned with HPLC-grade methanol (VWR International). Then 1mL of EA was added to the remaining sample (to maximize extraction efficiency) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The dried extracts were reconstituted in 60 μL of 11.4 mg/mL MOC in anhydrous pyridine (VWR International, Radnor, PA, USA), vortexed for 5 min ...
-
bioRxiv - Microbiology 2023Quote: ... we added another 0.5 ml PBS to the tubes that later were sonicated in the water bath of an ultrasonic cleaner (USC200TH, VWR International) for 5 min at 45 kHz ...