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Citations for VWR :
1401 - 1450 of 1904 citations for 1 Benzyl 4 5 benzyloxy 6 methoxy 1 indanone 2 ylidenyl methylpiperidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... in 1X PBS at 4°C before embedding them in Optimal Cutting Temperature (OCT) Compound (VWR Chemicals) and snap freezing them with isopentane on dry ice ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 48-hour incubation at 4°C in 50% optimal cutting temperature (OCT; VWR, #C361603E)/30% sucrose/phosphate buffer solution prior to freezing in 100% OCT using liquid nitrogen-cooled 2-methylbutane (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in cold PBS/0.04% BSA and fixed by adding 4 volumes 100% Methanol (VWR) and incubation for 30 min at -20 °C ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a centrifugation (10 minutes, 16.100 x g, 4°C, MicroStar 17R centrifuge, VWR, Darmstadt, Germany) for sample clearance ...
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bioRxiv - Systems Biology 2023Quote: ... The inoculum was then streaked onto XLT-4 containing 64 µg/mL nalidixic acid (VWR; Lot:21J285302) and incubated overnight at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Organoids were fixed in 4% PFA and embedded in Tissue-Tek® O.C.T (VWR, cat # 25608-930). Slides of cryosectioned organoids were blocked in 5% normal donkey serum (Jackson Immuno Research ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed using 4% paraformaldehyde for 10 minutes on the rocker (VWR analog rocker 10127-876). Fixed cells were washes once with PBS ...
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bioRxiv - Bioengineering 2022Quote: ... VWR Spinbar Micro Stir Bars and 4-ml Amber Borosilicate Glass sample vials were purchased from VWR.
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bioRxiv - Microbiology 2024Quote: ... 1e8 bacteria from each strain were pelleted and resuspended in 500 μL 4% paraformaldehyde (VWR, AAJ61899-AP) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... they were transcardially perfused with cold (4 °C) sterile saline and then 10% formalin (VWR, Radnor, PA). The brains of the mice were extracted from the skull ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All evolution was conducted at 30°C in 128μL of YPD (a rich laboratory media; 1% Bacto yeast extract (VWR #90000– 722), 2% Bacto peptone (VWR #90000–368) ...
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bioRxiv - Immunology 2020Quote: RNA was extracted using a modified phenol-chloroform protocol (Chomczynski and Sacchi, 2006) with 1-Bromo-3-chloropropane and Isopropanol (Sigma and VWR, respectively). Total RNA was quantified and assessed for quality and integrity by Bioanalyser (RNA Pico 6000 Chip ...
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bioRxiv - Molecular Biology 2021Quote: We prepared Aβ1-42 oligomers (oAβ) and fibrils (fAβ) following Evans (2006) and Ryan et al (2010) using siliconized polypropylene tubes (VWR #4165SL). We used Aβ1-42 to test the effects of the hybrid protein because it is more predominant in AD plaques (Nirmalraj et al. ...
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bioRxiv - Bioengineering 2021Quote: ... Stained tissue sections were washed in 0.1% Triton-X/PBS at RT for 1 hour and mounted on a poly-L-lysine coated coverglass (VWR, 48393-241) for imaging (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... Individual CNSs were sonified with five 0.3 s pulses at 1 Hz using a Branson Sonifier 450 (VWR Scientific, Winchester, PA) in Laemmli buffer (Bio-Rad ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Coating was performed for 1 h with 15 μg/ml of fibronectin or with 3.5 μg/cm2 Cell-Tak (VWR, 734-1081) diluted in 0.1 M sodium bicarbonate ...
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bioRxiv - Genetics 2022Quote: ... The slide was then transferred to 37°C for 1 min before being immersed and fixated in methanol (VWR EU, 20847.307) at -20°C for 30 min ...
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bioRxiv - Plant Biology 2021Quote: ... and the seeds were removed with forceps and put in a drop of clearing solution (1 vol glycerol / 7 vol chloral hydrate liquid solution, VWR Chemicals) between a slide and a coverslip ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...
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bioRxiv - Bioengineering 2022Quote: ... containing 20% FBS (Wisent, 80450, lot 115714), 1% Penicillin-Streptomycin solution (Wisent, 450-201-EL) and 2,5 ng/mL bFGF (VWR, 10821-962) in a 37°C ...
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bioRxiv - Immunology 2020Quote: ... the monocytes were harvested and the pellet resuspended in differentiation media (RPMI-1640 supplemented with 1% FBS H.I., Penicillin/Streptomycin (P/S, VWR, Radnor, PA) and 100 ng/mL phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Genomics 2020Quote: Single cell suspensions in PBS with 2% FBS were stained by incubating for 15 minutes at room temperature protected from light with anti-human PTPRC (CD45) monoclonal antibody conjugated to FITC (1:200 dilution, VWR #ABNOMAB12230), anti-human EPCAM antibody conjugated to PE (1:50 dilution ...
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bioRxiv - Biophysics 2023Quote: ... The cell constants for individual electrode devices and buffer conductivities were measured using a lock-in amplifier as previously described and a 500 μS cm−1 conductivity standard (VWR, UK).14 The electrophoresis data was analysed using software written in Python.17 Linear fits to the slope of the plots of velocity against electric field ...
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bioRxiv - Biophysics 2022Quote: ... the purified and concentrated protein was incubated with 1:3 protein to biotin ratio of 1mM NHS-PEG4-Biotin (VWR #PI21362) for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mL of supernatant was transferred to a borosilicate glass culture tube pre-cleaned with HPLC-grade methanol (VWR International). Then 1mL of EA was added to the remaining sample (to maximize extraction efficiency) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were visualised on a 1% agarose gel (run for 30 minutes at 135 V) with GelGreen (VWR, 730-1535) using an ImageQuant™ LAS 500 (GE Lifesciences) ...
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bioRxiv - Plant Biology 2023Quote: ... The seeds on their replum were then put on a slide with a drop of clearing solution (1 vol glycerol/7 vol chloral hydrate liquid solution, VWR Chemicals). The seeds were detached from the replum and the slide was covered with a coverslip and kept in the dark for at least 24h in a cold room (4°C) ...
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bioRxiv - Developmental Biology 2023Quote: ... for 50 ms at 1 s intervals using electrodes (diameter 3 mm; Sonidel CUY650P3) and ECM-830 BTX square wave electroporator (VWR international). After electroporation ...
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bioRxiv - Genomics 2023Quote: ... then incubated on rollers at room temperature for 1 hour with an anti-mouse secondary antibody conjugated to horseradish peroxidase (VWR, NXA931). After four further washes for 10 min each (2 x blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...
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bioRxiv - Biophysics 2023Quote: ... and 1%(v/v) ammonium persulfate (APS) for 60 minutes at RT on plasma cleaned glass cover slips (No.1, 25mm Ø, VWR) that were pre-treated with 3-aminoproyltriethoxysiliane (APTES ...
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bioRxiv - Genetics 2023Quote: ... hTERT RPE-1 cells (ATCC, CRL-4000) were maintained in DMEM:F-12 media (ATCC, 30-2006) supplemented with 10% FBS (VWR, 97068-091) and 1% Pen-Strep (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 100 mg of minceate was then suspended in 1 mL ice-cold 0.3 M sucrose (Fisher, cat# BP220) in H2O supplemented with protease inhibitor cocktail (VWR, cat# M250) at 1:100 (1:10 w/v suspension) ...
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bioRxiv - Microbiology 2024Quote: ... aureus strains were generated as previously described 101 and transformed with ∼5 µg of the pIMAY construct using the BioRad Gene Pulser Xcell electroporation system and 1 mm disposable electroporation cuvettes (VWR Signature). The pulse was 21kV/cm ...
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bioRxiv - Physiology 2024Quote: ... the cells were washed twice with 1x DPBS and stained with secondary antibodies: Alexa Fluor® 594 AffiniPure Donkey Anti-Rabbit IgG (H+L) (Cat #102649-732, 1:300, VWR), and Alexa Fluor® 488 AffiniPure Donkey Anti-Rabbit IgG (H+L ...
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bioRxiv - Genomics 2024Quote: ... 100 uL HoxB8 neutrophils (1 × 106 cells/mL) were seeded into an 8-well Nunc Lab-Tek II Chamber Slide (VWR international) coated with 2% poly-L-lysine (MilliporeSigma) ...
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bioRxiv - Systems Biology 2024Quote: ... All antibodies were diluted at 1:100 in FACS buffer [PBS with 10% fetal bovine serum (FBS, VWR, MDTC35-016-CV) containing 1% Penicillin-Streptomycin solution (P/S ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells from four 80% confluent 10 cm Petri dishes were transfected with 0.9μg VSV-G envelope expressing plasmid pMD2 and 9 μg psPAX2 packaging vectors and 9μg of the gRNA library DNA in the presence of 113.4μL Polyethylenimine - PEI (VWR International, 1 mg/mL) per plate ...
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bioRxiv - Cell Biology 2024Quote: ... followed by resuspension in culture medium (DMEM low glucose, 10% HI-FBS, 1% Penicillin/Streptomycin) and filtered through a 40 μm cell strainer (VWR, USA). The obtained single cell suspensions of ovarian primary cells were seeded either for monolayer 2D culture (1.5 × 106 cells/well ...
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bioRxiv - Microbiology 2023Quote: ... Six to seven grams (6 ml) of soil was transferred to each well of the 12-well microtiter plates (VWR American cat no.:10861-556, USA), containing three one-millimeter holes in the bottom of all wells for drainage ...
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bioRxiv - Microbiology 2020Quote: ... The mixture was gently spread on two parallel SM/5 plates using a plastic rake (VWR), resulting in technical replicates ...
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bioRxiv - Cancer Biology 2021Quote: ... subcutaneous tumor fragments weighing 5–15 mg were homogenized using Kontes tubes with disposable pestles (VWR) in ice-cold 80:20 methanol:water (v/v) ...
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bioRxiv - Cell Biology 2021Quote: ... fixed platelet subpopulations were centrifuged onto poly-l-lysine coverslips (VWR; 600 x g, 5 minutes), permeabilized with 0.2% Triton ...
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bioRxiv - Cancer Biology 2022Quote: ... medium was replaced by 60 μL of 5 mg/mL Collagen Type I (VWR, 734-1085) in 1M NaOH and 1X DMEM/F12 ...
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bioRxiv - Plant Biology 2020Quote: ... plants were harvested and meristems were stained with 5 μM SynaptoRed membrane dye (VWR 80510-682) in DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... and gut (n=5-10 per condition) organoids were fixed in 10% neutral buffered formalin (VWR) with 0.03% eosin (Newcomer Supply ...
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bioRxiv - Systems Biology 2023Quote: ... SPE columns were washed with icecold H2 O (5 mL, MS grade, VWR International, Vienna, Austria) and elution of analytes was carried out with ice-cold MeOH (500 μL ...
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bioRxiv - Microbiology 2022Quote: ... All cells were maintained at 37 °C in a 5% CO2 humidified incubator (VWR, Mississauga, Ontario). For subculturing ...
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bioRxiv - Microbiology 2024Quote: The MIC and minimum bactericidal concentrations (MBC) of benzalkonium chloride (BZC) (CAS 68391-01-5, VWR) were determined for representative sequenced isolates (n=45 ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (3 x 5 min) and mounted on slides (Superfrost Plus, VWR) with mounting medium (Fluoromount ...