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Citations for VWR :
1251 - 1300 of 1525 citations for 1R 2S 6R 7S 4 4 DIMETHYL 3 5 DIOXA 8 AZATRICYCLO 5.2.1.0 2 6 DECAN 9 ONE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated in blocking solution [5% milk in PBST (PBS (pH 7.4) with 0.1% Tween-20 (VWR))] for 2 h and probed overnight with the following primary antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized in 0.5% Triton X-100 (MilliporeSigma) for 10 min and blocked with 5% bovine serum albumin (VWR) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was diluted 1:5 in PBS and added to 384 well ELISA plates (VWR, cat. CA62409-064) that had been previously coated with 1 𝜇g/mL NeutrAvidin (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Beads were washed 4X in Kischkel buffer + 2 μg/ml Aprotinin (VWR, Radnor, Pennsylvania, 97062-752) + 1 μg/ml Pepstatin (VWR 97063-246 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Caco-2 cells were cultured in EMEM (ATCC, 30-2003) with 20% FBS (VWR 89510-186), 1% Pen/Strep (Life Technologies 15140122) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Supernatant was supplemented with 2 mM calcium chloride and filtered by a glass microfiber filter (VWR). Samples were then loaded by gravity flow onto 5 ml anti-protein C antibody affinity resin ...
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bioRxiv - Microbiology 2020Quote: ... in antibiotic-free LB and 2 mL-deep-96-well plates in checkered pattern (VWR International). Plates were incubated at 37°C with 700 rpm shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Immunology 2020Quote: ... were separately stored in 2 mL pre-filled 2.4mm metal beads tubes (VWR International, Radnor, PA), and quickly snap-frozen in liquid nitrogen (40) ...
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bioRxiv - Neuroscience 2024Quote: ... pre-chilled 2 ml Pre-Filled Bead Tubes with 2.8 mm Ceramic Beads (VWR, Radnor, PA), and weighed ...
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bioRxiv - Cell Biology 2022Quote: ... glucose was replaced with 2% (w/v) galactose (ITW reagents) or 1.5% (w/v) raffinose (VWR), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... mice expressing hM4D(Gi) were intraperitoneally injected with 2 mg/kg CNO (89160; VWR International, LLC) and returned to their home cages ...
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bioRxiv - Microbiology 2024Quote: ... 2 mL of DMEM infection media containing 0.2% w/v bovine serum albumin (BSA; VWR International) and 1 μg/mL L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μl cell suspension was loaded under a 22 × 22 mm glass coverslip (VWR, thickness: 1.5). Fluorescence images in Figures 1b ...
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bioRxiv - Molecular Biology 2023Quote: ... we seeded 2×104 ESCs per well of a Geltrex-coated 12-well plate for subsequent RT-PCR and RT-qPCR analyses and 1-3×103 cells per well of a 12-well plate containing a Geltrex-coated 18-mm glass coverslip (VWR, cat# 631-1580) for immunostaining ...
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bioRxiv - Cancer Biology 2024Quote: ... Supernatant was removed and replaced with 500 μL of ACK (ammonium-chloride-potassium) lysis buffer per sample to remove red blood cell contamination (3 min incubation at 37°C; VWR cat#10128-808). Reaction was quenched with 10 mL of cold DMEM/F12-Advanced media supplemented with 10% FBS,1% L-glutamine ...
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bioRxiv - Bioengineering 2020Quote: ... bacteria were removed from media by centrifugation at 12,000g for 5 min and the supernatants were transferred to black flat-bottomed microtiter plates (VWR).
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bioRxiv - Microbiology 2021Quote: ... MD) and maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS, VWR) and 1% penicillin-streptomycin-glutamine (PSG ...
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bioRxiv - Synthetic Biology 2020Quote: ... centrifuged for 5 min at 4000 x g-1 and filtered over a 0.2 µm cellulose acetate filter (VWR). 1 ml aliquots were concentrated in a speed-vac (Eppendorf ...
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bioRxiv - Neuroscience 2020Quote: ... into the LC (AP:−5.4 mm, ML: ±1.2 mm, DV, −4.0 mm) with a 5 µL Hamilton syringe (VWR, Radnor, PA) and stereotaxic injector pump (Stoelting ...
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bioRxiv - Cancer Biology 2020Quote: ... through injection of 5×105 67NR murine luminal breast cancer cells in 100 μL of serum-free RPMI (VWR). 67NR cells are locally invasive but non-metastatic ...
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bioRxiv - Neuroscience 2020Quote: ... Leads were connected to the animal’s front paws and right hind paw via Skintact FS-TB1-5-Gel ECG Electrode (VWR) as shown in Figure 1.
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bioRxiv - Molecular Biology 2023Quote: ... Benzonase nuclease was purchased from Sigma (E1014-25KU) and streptavidine sepharose high performance beads (5 mL) was purchased from VWR (GE Healthcare 17-5113-01). OMIX C18 tips (100 µL ...
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bioRxiv - Microbiology 2023Quote: ... XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] solution (0.5 mg XTT/mL 1× PBS with 25 µM menadione) (XTT sodium salt, VWR) was added at 150 µl per well and incubated at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of a blocking/permeabilization buffer (5% BSA and 0.2% Triton in PBS; BSA from Sigma; TritonX-100 from VWR) was added and cells were incubated for 1h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... after which they were permeabilized in 0.5% Triton X-100 (MilliporeSigma) for 10 minutes and blocked with 5% bovine serum albumin (VWR) for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... TGIRT was dissociated from the RNA-DNA duplexes by addition of 1 μL 5 M NaOH (VWR BDH7225-1) with incubation at 95 °C for 3 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysate was filtered through a 0.45 µm filter and cohesin purified in the AKTA system using a 5 mL (HisTrap) TALON column (VWR). The column was washed with 20 column volumes of buffer B (20 mM Tris PH7.5 ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then blocked with 5% nonfat dairy milk in Tris-buffered saline containing 0.1% Tween 20 (VWR, 0777), and incubated overnight with the following primary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were blocked for 90 minutes at room temperature in a humidified chamber with a blocking buffer consisting of 0.1% Triton X-100 and 5% Bovine Serum Albumin (VWR). All the antibodies used in this study and their concentrations are listed in File S6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT on a rocker with a blocking buffer consisting of 0.1% Triton X-100 and 5% goat serum (VWR). The organoids were incubated overnight in the primary antibodies (File S6 ...
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bioRxiv - Developmental Biology 2022Quote: ... Later blocking was done for 1hour at RT in a humidified chamber with a blocking buffer consisting of 0.1% Triton X-100 and 5% Bovine Serum Albumin (VWR). The organoids were incubated in primary antibodies (File S6 ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% (vol/vol) fetal bovine serum (FBS, VWR) and 1% penicillin–streptomycin–glutamine (PSG ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a 1:5 volumetric ratio (chloroform:Trizol) for aqueous separation of RNA via centrifugation in heavy gel phase-lock tubes (VWR). Aqueous phase was transferred into isopropanol followed by RNA purification using a Qiagen RNeasy Mini Kit as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... serial dilutions were made for each suspension ranging from 10-3 to 10-7 and 100 μL of each of these were streaked on a 5% sheep’s blood in tryptic soy agar plate (VWR) and incubated at 37°C for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then blocked in a solution containing 5% skimmed milk powder in Tris-buffered saline with 0.1% Tween-20 (VWR) for 1 hour at ambient temperature.
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bioRxiv - Microbiology 2024Quote: ... 2 ml of the culture was collected through centrifugation (5 min, 8000 rpm) and the supernatant filtered (0.22-μm filter; VWR). Secreted proteins in the supernatant were then precipitated using 10% ice cold trichloroacetic acid (TCA ...
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bioRxiv - Microbiology 2021Quote: ... The cells were washed off the filter with an ice-cold freshly prepared mixture of methanol/ethanol/chloroform (1:3:1) (ethanol LiChroSolv©, Merck, Darmstadt, Germany; chloroform HiPerSolv©, VWR, Dresden, Germany). Extracts were treated in an ultrasonic bath for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... Four sets of dilutions (100 to 10-3) of the filtered lysates were created in standard 96 well plates (VWR, cat. no. 25381-056), then arrayed in quadrants of a single 384-well plate ...
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bioRxiv - Genetics 2021Quote: ... Amplified emulsion mixture was broken and purified by adding 1 mL of 2-butanol (VWR, AA43315-AK), 50 μL of AMPure XP SPRI (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... Transgene expression was induced with 24-hour incubation in media supplemented with 2 μg/ml doxycycline (VWR). SiRNA-mediated depletion of endogenous ESCO2 was done with 20 nM siRNA (Dharmacon ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplified cPCR products were examined on 2% agarose gels stained with GelRed (VWR International PBI, Milan, Italy) and visualized on a GelLogic 100 gel documentation system (Kodak ...
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bioRxiv - Cancer Biology 2022Quote: ... for 2 hours at 56°C and 50 μg of RNase A (VWR International, Radnor, PA, USA) for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... spectinomycin (100 µg/ml) and 0.2% arabinose in a 2 ml 96-well deep well block (VWR), sealed with breathable film (VWR ...
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bioRxiv - Neuroscience 2020Quote: ... Cells used for in vitro electrophysiology experiments were grown on glass coverslips (12×12mm, No.2, VWR). Patch-clamp experiments were performed in aCSF consisting of (in mM) ...
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bioRxiv - Cancer Biology 2024Quote: ... or tissue culture-treated T-25 flasks (2×106 cells/flask; Avantor/VWR, Cat. No. 10861-568) under the growth conditions described above ...
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bioRxiv - Cell Biology 2023Quote: ... slabs were optically cleared using a 1:2 mixture of benzyl alcohol (Cat. No. 109626; VWR, Sweden) and benzyl benzoate (BABB ...
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bioRxiv - Biophysics 2023Quote: ... were soaked for 1 hour at ~ 60°C in 2% v/v cleaning detergent (MICRO-90, VWR), thoroughly rinsed with 18.2 MΩ ultra-pure water ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL cell suspension was loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5).