Labshake search
Citations for VWR :
1151 - 1200 of 1203 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... NPCs were plated in 24 well CELLSTAR® cell culture plates (for bulk-RNA-seq. and live imaging; VWR, 82050-892), µ-Slide 8 Well (for Video S1. ...
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bioRxiv - Molecular Biology 2023Quote: ... animals of the indicated genotype were washed out of wells on a 6-well plate at the indicated timepoints with M9+0.05% gelatin (VWR, 97062-620), transferred to a 1.5 ml tube and washed twice more with M9+0.05% gelatin ...
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bioRxiv - Synthetic Biology 2022Quote: ... boulardii strains were incubated in 2 mL DELFT medium supplemented with 20 mg/L uracil in a 24-deep well plate (Axygen®, VWR) with a sandwich cover (Enzyscreen) ...
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bioRxiv - Plant Biology 2024Quote: ... Apogee grain were left for 2 days at 5°C in water for imbibition before being placed individually onto cotton wool plugs in a 24-well tissue culture plate (VWR, USA) and left to germinate for 3 days under high humidity conditions (<90% relative humidity ...
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bioRxiv - Physiology 2024Quote: ... they were individually transferred to single wells of a 96-well single-cell-sequencing plate from VWR (Cat. No. 82006-636) with 2 ul of RNAse inhibitor mix (containing 0.12 ul Triton X-100 10 % ...
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bioRxiv - Molecular Biology 2024Quote: SC-islets or human donor islets were hand-picked and transferred to 6-well non-TC treated plates (VWR, #10861-554) to undergo the treatment condition outlined above ...
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bioRxiv - Immunology 2024Quote: ... mouse bone marrow-derived macrophages were harvested as previously described (38) and cultured in the same media on non-tissue culture polystyrene plates (VWR International). Similarly ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μL of enzymés dilutions were incubated for 10 min at each temperature point into non-skirted 96-well PCR plates (VWR, #732-2387) sealed with thermoresistant adhesive sheets (Thermos scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 100 µL of each evolving population was mixed with 100 µL of 30% (v/v) glycerol and stored at ™80°C in shallow 96-well plates sealed with Adhesive Foil (VWR), following the same layout as the evolution plates for future analysis ...
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bioRxiv - Bioengineering 2021Quote: ... A 15-µl aliquot of the master mix was combined with 10 µl of sample in a 96-well PCR plate (VWR, 83007-374). The plate was mixed on a Qiagen TissueLyser II for two 25-second intervals at 13 Hz then centrifuged for 2 minutes at 1000 xg.
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bioRxiv - Bioengineering 2021Quote: ... The cells were plated to ∼2 x 105 cells per well in a 6-well VWR tissue culture-treated plates (VWR, Suwanee, GA) and incubated ∼12 hours (overnight) ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cultures expressing CcaS-CcaR system variants were cultured in darkness for 12-14 h in LB (1% Tryptone, 0.5% Yeast Extract, 1% Sodium Chloride) with appropriate antibiotics in 96 well plates (VWR, Cat#732-3802), with one 3 mm glass bead and 750 μl media per well at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes at room temperature with gentle agitation on a plate rocker and mounted on microscope slides (VWR REF# 48311-703) with mounting medium containing DAPI ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 2000 cells per well in a volume of 100 µl were seeded on flat-bottomed 96-well plates (VWR, Radnor, USA) and incubated in a 95% humidified atmosphere with 5% CO2 at 37 °C for 24 h ...
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bioRxiv - Bioengineering 2021Quote: Plant or mammalian produced SARS-CoV-2 RBD protein were diluted in sterile 1X PBS to 2 μg/mL and coated onto a 96-well plates (VWR #62402-959) (50 μL /well ...
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bioRxiv - Microbiology 2024Quote: ... 1 mL of M9 was added to the plate and the bacterial lawns were scraped with a sterile L spreader (VWR 490007-358). Bacterial slurry was pipetted and centrifuged at 13000 x g for 4 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... Single clones were picked from plated into 1 mL of TB media containing 100 μg/mL Ampicillin in 2 mL 96-well plates (VWR, 75870-796) and grown overnight in a 37 °C rotating shaker ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 21°C in shaking culture in HL5 medium (Formedium, Norfolk, England) and on SM/5 agar plates (2 g/L glucose (VWR, Solon, OH), 2 g/L bacto peptone (Becton ...
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bioRxiv - Plant Biology 2023Quote: ... from accessions grown in UC Davis or UC Riverside greenhouses and floated overnight in 1 mL deionized water in a 24-well tissue culture plate (VWR #10062-896) with a plastic lid to prevent evaporation of the water ...
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bioRxiv - Neuroscience 2023Quote: ... and BHK-B19L cells (see above) were plated in 10 cm plates coated in poly-L-lysine in normal medium (10% fetal bovine serum (VWR 16777-014) and antibiotic-antimycotic (Thermo 15240096 ...
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bioRxiv - Genetics 2023Quote: ... A 750 μL suspension of each sample was then transferred to a 96-well filter plate (Norgen, #40008) where the media was eliminated by applying vacuum (VWR, #16003-836). Immediately after the media was eliminated ...
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bioRxiv - Genomics 2023Quote: ... cells were harvested by filtration in 96-well filter plate (Norgen, #40008) where the media was eliminated by applying vacuum (VWR, #16003-836). The filter plates were immediately flash frozen in liquid nitrogen and stored at -80°C.
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bioRxiv - Genetics 2023Quote: ... Small pieces of cotton wool soaked with sterile water were placed in each well of a 24-well tissue culture plate (VWR International, US). One wheat seed was placed with the crease facing downwards into each well ...
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bioRxiv - Microbiology 2023Quote: ... were transfected into single wells (3.5 cm) of the Corning Costar 6-well cell culture plate containing HEK293T/17 cells using the JetPrime Transfection reagent (VWR, Randor, PA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were seeded at 1×106 cells/well in 24-well plates and maintained in X-VIVO-15™ media (CA12001-988, VWR, USA). Four treatment groups were used ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 10 µL 2:1 CHCl3/MeOH and spotted in 2.5 µL aliquots on a TLC plate (Alugram, Xtra Sil G/UV254, 0.2 mm/silica gel 60; VWR, Cat. No. 552-1006).
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bioRxiv - Microbiology 2024Quote: ... The last column of the plate was not coated since it contained the medium alone to allow the addition of sodium sulfite (VWR 0628-500g) during the run ...
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bioRxiv - Immunology 2024Quote: Single cells prepared from tumor tissues were counted and 2-5 million cells were plated on a 96-well plate (VWR, round botom) and washed with PBS ...
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bioRxiv - Bioengineering 2024Quote: ... cell line -PGP-1 (the Personal Genome Project-1) used for this study were propagated on tissue culture plates coated with Matrigel (VWR; 75796-278) by using mTeSR1 (StemCell Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Solutions were mixed by shaking the compound plate for 1 min at room temperature at 1200 rpm using a microplate shaker (cat. 12620-926, VWR, West Chester, PA). A typical plate layout map used for screening experiments is shown in Figure 1 ...
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bioRxiv - Bioengineering 2021Quote: ... Microtiter plates were filled with 160 μl of bloodmeal per well and sealed with a membrane (Parafilm M, PM999, VWR, Amsterdam, the Netherlands) that was stretched to about 250% its original dimensions in both directions using a custom build device (Figure S1A ...
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bioRxiv - Cancer Biology 2021Quote: 1×105 A549 or H460 cells in 200 µl of serum-free growth medium were seeded in the insert of a transwell plate with 8 μm pores (VWR International, Radnor, PA). These cells were coincubated with AvFc or CTX at 30 nM for 2 hours at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... from control and treatment cultures were harvested at both 24- and 48-hour time points and mixed with 400μL of culture media in a 96-deep well storage plate (VWR International, Radnor, PA, USA) and stored frozen at −20°C for cytokine assays by ELISA ...
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bioRxiv - Microbiology 2021Quote: Antibiotic tolerance assays using co-cultures were performed using a membrane-separated 12-well tissue plate cultures containing 0.1 μm pore PET membranes (VWR, Cat. No. 10769–226), following previously protocols (Meirelles et al. ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of the enriched seawater and 100 μL of the diatom-concentrated seawater were then inoculated on agar plates (1% agar – VWR Agar for bacteriology) supplemented with Marine Broth 2216 (VWR ...
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bioRxiv - Genomics 2022Quote: ... HeLa S3 or HeLa S3 + TOP2A- Venus cell linescells were either grown or spun onto 22×22mm No 1.5 coverslips in 6-well plates (VWR Cat No 48366-277). (cell cycle analysis experiments ...
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bioRxiv - Microbiology 2024Quote: The bacterial strains were revived by streaking a loopful of frozen glycerol stock on Brain Heart Infusion (BHI) agar plates (15 g/L Agar, 37 g/L BHI Broth, VWR Life Science, USA) and incubated at 37±2 °C overnight ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... A single colony of bacteria from each plate was cultured under anaerobic conditions (Anaerobic Hungate Culture Tubes with air displaced by CO2, VWR, Radnor, PA, USA), shaking at 35°C for 3 nights and was then retained at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 200 µL aliquots were then transferred to the wells of a NUNC flat-bottomed 96-well microtiter plate (VWR International GMBH, Darmstadt, Germany). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... cells were passaged 1:10 to a new 96-well Matrigel-coated plate for gDNA extraction with 20 μL of Quick Extract gDNA lysis solution (Catalog: QE09050, Bio-Search Technologies, VWR, Radnor, PA, USA). The remaining cells were overlaid with 50 μL of 20% mTeSR1 ...
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bioRxiv - Cell Biology 2024Quote: All types of cells were plated at a density of 250 cells/mm2 and cultivated in tissue culture plates (VWR, Radnor, PA, USA) or glass-bottom dishes (Greiner Bio-One ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells from all SynOMICS deletion and integration experiments were plated on agar plates in 14.5 cm diameter Greiner Bio-One Petri dishes (VWR Catalog No. 82050-600). Following colony formation ...
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bioRxiv - Synthetic Biology 2024Quote: ... cDNA were then diluted and added to a qPCR compatible 96-well plate (Fisher, 4346907) together with qPCR master mix: SENSIMIX SYBR HI-ROX (VWR, 490017-866 / 868) and primers targeting human or mouse Rai1 gene ...
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bioRxiv - Synthetic Biology 2024Quote: Transformed colonies were scraped from the agar plate of the sample group into 10 mL of LB medium supplemented with 50 µg/mL kanamycin (VWR, Cat# 0408-10G) in a sterile 50 mL polypropylene conical tube (Corning ...
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bioRxiv - Microbiology 2020Quote: ... The plates were incubated for four hours at 37 °C under shaking conditions (175 rpm) using a benchtop incubator (VWR® incubator orbital shaker). The 96-well plate was kept inside an airtight plastic container with several wet paper towels to maintain high humidity attached to the shaker ...
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bioRxiv - Microbiology 2020Quote: ... co-culture experiments were performed using membrane-separated 12-well tissue plate cultures containing 0.1 µm pore PET membranes (VWR® Cat. No. 10769-226). Briefly ...
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bioRxiv - Immunology 2022Quote: Splenic T-lymphocytes were counted and plated at an optimal seeding density of 8×104 cells per 100 µL in 96 well Microtitration ImageLock plates (VWR, #4379, Radnor, PA, USA). The cells were cultured with anti-CD3 and anti-CD28 activation beads and imaged over the course of 96hrs in the Essen Bioscience IncuCyte S3 Live-Cell imager and analysis system.
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bioRxiv - Synthetic Biology 2022Quote: ... 1 μL volumes are taken from this tray using a multipipettor and used to inoculate wells of a 96-well plate (VWR, catalog number 10062-900) with 200μL volumes of H media ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Viable embryos at the 2-cell stage (0.75 hours post fertilization (hpf)) were sorted into 4 replicates of 10 embryos within 24-well plates (VWR international, LLC Radnor, PA, USA). Replicates were exposed to 1 mL of vehicle (0.1% DMSO ...
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bioRxiv - Microbiology 2024Quote: ... Four sets of dilutions (100 to 10-3) of the filtered lysates were created in standard 96 well plates (VWR, cat. no. 25381-056), then arrayed in quadrants of a single 384-well plate ...